PRJDA46243 Metatranscriptomic analysis for eukaryotic functional genes in forest soil To explore the eukaryotic functional genes, we demonstrated metatranscriptomic approach using polyadenylated mRNA extracted from an enriched forest soil sample. A total of 93,415,467 bp of cDNA using GS-FLX was sequenced. As a result of assembly, 56,084 of putative protein-coding sequence (CDS) fragments were identified. This metatranscriptomic approach allowed a first glimpse into the in situ activity of the much number of the novel functional genes from eukaryote. Metatranscriptomic analysis for eukaryotic functional genes in forest soil bioproject PRJDA46243 PRJDA46243 true genomeprj 46243 46243 false The aim of this study is the exploration of the novel eukaryotic functional genes from an environmental sample using metatranscriptomic approach. This method is widely applicable to the provision of novel eukaryotic enzymes and proteins of potential industrial or medical use. For the extraction of environmental RNA after enrichment with wheat bran, the sample was collected from the forest humic soil in the site of AIST. The extraction of total RNA and the purification of polyadenylated eukaryotic mRNA from the enriched soil sample were used to commercially available kit, respectively. The synthesized cDNA sample from the purified mRNA was outsourced to Dragon Genomics Center for 454 GS FLX sequencing.