PRJNA39577 Genome reconstruction of previously uncultivated lineages of thermophilic archaeotes A first composite genome sequence of "Caldiarchaeum subterraneus" that belongs to the previously uncultivated thermophilic creanrchaeotic lineage was successfully reconstructed. Subsurface mine microbial mat metagenome bioproject PRJNA39577 PRJNA39577 true genomeprj 39577 39577 false We selected putative archaeal genome fragments from the metagenomic library that was constructed from DNA assemblages isolated from a microbial mat at a geothermal water stream in a Japanese subsurface gold mine. Total of selected 132 fosmids were grouped into each 13 or 20 fosmid and each group was analyzed in a half plate of 454 GS20. 18 fosmids were analyzed by Sanger sequencing. A composite archaeal genome was reconstructed from 91 complete or partial fosmid sequences. Genome Sequences Analyzed by JAMSTEC http://www.jamstec.go.jp/gbrowser/cgi-bin/top.cgi pubmed 21169198