<?xml version="1.0" encoding="UTF-8" standalone="yes"?>
<EXPERIMENT_SET>
    <EXPERIMENT accession="DRX001074" center_name="KYOTO_AG" alias="DRX001074">
        <TITLE>metagenomic analysis on 16S rDNA</TITLE>
        <STUDY_REF accession="DRP000487" refcenter="KYOTO_AG" refname="DRP000487">
            <IDENTIFIERS>
                <PRIMARY_ID label="BioProject ID">PRJDA73169</PRIMARY_ID>
            </IDENTIFIERS>
        </STUDY_REF>
        <DESIGN>
            <DESIGN_DESCRIPTION>none provided</DESIGN_DESCRIPTION>
            <SAMPLE_DESCRIPTOR accession="DRS001056" refcenter="KYOTO_AG" refname="DRS001056">
                <IDENTIFIERS>
                    <PRIMARY_ID label="BioSample ID">SAMD00009685</PRIMARY_ID>
                </IDENTIFIERS>
            </SAMPLE_DESCRIPTOR>
            <LIBRARY_DESCRIPTOR>
                <LIBRARY_NAME>16S rDNA PCR amplicons</LIBRARY_NAME>
                <LIBRARY_STRATEGY>AMPLICON</LIBRARY_STRATEGY>
                <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE>
                <LIBRARY_SELECTION>PCR</LIBRARY_SELECTION>
                <LIBRARY_LAYOUT>
                    <SINGLE/>
                </LIBRARY_LAYOUT>
                <TARGETED_LOCI>
                    <LOCUS locus_name="16S rRNA">
                        <PROBE_SET>
                            <DB></DB>
                            <ID></ID>
                        </PROBE_SET>
                    </LOCUS>
                </TARGETED_LOCI>
                <LIBRARY_CONSTRUCTION_PROTOCOL>tag_sequence sample
AACGCACGCTAG Lambir_ridge1
ACACTGTTCATG Lambir_ridge2
ACCAGACGATGC Lambir_ridge3
ACGCTCATGGAT Lambir_ridge4
ACTCACGGTATG Lambir_ridge5
AGACCGTCAGAC Lambir_valley1
AGCACGAGCCTA Lambir_valley2
ACAGACCACTCA Lambir_valley3
ACCAGCGACTAG Lambir_valley4
ACGGATCGTCAG Lambir_valley5
AGCTTGACAGCT Bakam_remnant1
AACTGTGCGTAC Bakam_remnant2
ACCGCAGAGTCA Bakam_remnant3
ACGGTGAGTGTC Bakam_remnant4
ACTCGATTCGAT Bakam_remnant5
AGACTGCGTACT Bakam_secondary1
AGCAGTCGCGAT Bakam_secondary2
AGGACGCACTGT Bakam_secondary3
AAGAGATGTCGA Bakam_secondary4
ACAGCAGTGGTC Bakam_secondary5
ACGTACTCAGTG Bakam_burned1
ACTCGCACAGGA Bakam_burned2
AGAGAGCAAGTG Bakam_burned3
AGCATATGAGAG Bakam_burned4
AGGCTACACGAC Bakam_burned5
AAGCTGCAGTCG Ashiu1
ACAGCTAGCTTG Ashiu2
ACCTGTCTCTCT Ashiu3
ACGTCTGTAGCA Ashiu4
AGAGCAAGAGCA Ashiu5</LIBRARY_CONSTRUCTION_PROTOCOL>
            </LIBRARY_DESCRIPTOR>
            <SPOT_DESCRIPTOR>
                <SPOT_DECODE_SPEC>
                    <SPOT_LENGTH>800</SPOT_LENGTH>
                    <READ_SPEC>
                        <READ_INDEX>0</READ_INDEX>
                        <READ_CLASS>Technical Read</READ_CLASS>
                        <READ_TYPE>Adapter</READ_TYPE>
                        <BASE_COORD>1</BASE_COORD>
                    </READ_SPEC>
                    <READ_SPEC>
                        <READ_INDEX>1</READ_INDEX>
                        <READ_LABEL>barcode_tag</READ_LABEL>
                        <READ_CLASS>Technical Read</READ_CLASS>
                        <READ_TYPE>BarCode</READ_TYPE>
                        <EXPECTED_BASECALL_TABLE>
                            <BASECALL match_edge="full" max_mismatch="2" read_group_tag="AACGCACGCTAG">AACGCACGCTAG</BASECALL>
                            <BASECALL match_edge="full" max_mismatch="2" read_group_tag="ACACTGTTCATG">ACACTGTTCATG</BASECALL>
                            <BASECALL match_edge="full" max_mismatch="2" read_group_tag="ACCAGACGATGC">ACCAGACGATGC</BASECALL>
                            <BASECALL match_edge="full" max_mismatch="2" read_group_tag="ACGCTCATGGAT">ACGCTCATGGAT</BASECALL>
                            <BASECALL match_edge="full" max_mismatch="2" read_group_tag="ACTCACGGTATG">ACTCACGGTATG</BASECALL>
                            <BASECALL match_edge="full" max_mismatch="2" read_group_tag="AGACCGTCAGAC">AGACCGTCAGAC</BASECALL>
                            <BASECALL match_edge="full" max_mismatch="2" read_group_tag="AGCACGAGCCTA">AGCACGAGCCTA</BASECALL>
                            <BASECALL match_edge="full" max_mismatch="2" read_group_tag="ACAGACCACTCA">ACAGACCACTCA</BASECALL>
                            <BASECALL match_edge="full" max_mismatch="2" read_group_tag="ACCAGCGACTAG">ACCAGCGACTAG</BASECALL>
                            <BASECALL match_edge="full" max_mismatch="2" read_group_tag="ACGGATCGTCAG">ACGGATCGTCAG</BASECALL>
                            <BASECALL match_edge="full" max_mismatch="2" read_group_tag="AGCTTGACAGCT">AGCTTGACAGCT</BASECALL>
                            <BASECALL match_edge="full" max_mismatch="2" read_group_tag="AACTGTGCGTAC">AACTGTGCGTAC</BASECALL>
                            <BASECALL match_edge="full" max_mismatch="2" read_group_tag="ACCGCAGAGTCA">ACCGCAGAGTCA</BASECALL>
                            <BASECALL match_edge="full" max_mismatch="2" read_group_tag="ACGGTGAGTGTC">ACGGTGAGTGTC</BASECALL>
                            <BASECALL match_edge="full" max_mismatch="2" read_group_tag="ACTCGATTCGAT">ACTCGATTCGAT</BASECALL>
                            <BASECALL match_edge="full" max_mismatch="2" read_group_tag="AGACTGCGTACT">AGACTGCGTACT</BASECALL>
                            <BASECALL match_edge="full" max_mismatch="2" read_group_tag="AGCAGTCGCGAT">AGCAGTCGCGAT</BASECALL>
                            <BASECALL match_edge="full" max_mismatch="2" read_group_tag="AGGACGCACTGT">AGGACGCACTGT</BASECALL>
                            <BASECALL match_edge="full" max_mismatch="2" read_group_tag="AAGAGATGTCGA">AAGAGATGTCGA</BASECALL>
                            <BASECALL match_edge="full" max_mismatch="2" read_group_tag="ACAGCAGTGGTC">ACAGCAGTGGTC</BASECALL>
                            <BASECALL match_edge="full" max_mismatch="2" read_group_tag="ACGTACTCAGTG">ACGTACTCAGTG</BASECALL>
                            <BASECALL match_edge="full" max_mismatch="2" read_group_tag="ACTCGCACAGGA">ACTCGCACAGGA</BASECALL>
                            <BASECALL match_edge="full" max_mismatch="2" read_group_tag="AGAGAGCAAGTG">AGAGAGCAAGTG</BASECALL>
                            <BASECALL match_edge="full" max_mismatch="2" read_group_tag="AGCATATGAGAG">AGCATATGAGAG</BASECALL>
                            <BASECALL match_edge="full" max_mismatch="2" read_group_tag="AGGCTACACGAC">AGGCTACACGAC</BASECALL>
                            <BASECALL match_edge="full" max_mismatch="2" read_group_tag="AAGCTGCAGTCG">AAGCTGCAGTCG</BASECALL>
                            <BASECALL match_edge="full" max_mismatch="2" read_group_tag="ACAGCTAGCTTG">ACAGCTAGCTTG</BASECALL>
                            <BASECALL match_edge="full" max_mismatch="2" read_group_tag="ACCTGTCTCTCT">ACCTGTCTCTCT</BASECALL>
                            <BASECALL match_edge="full" max_mismatch="2" read_group_tag="ACGTCTGTAGCA">ACGTCTGTAGCA</BASECALL>
                            <BASECALL match_edge="full" max_mismatch="2" read_group_tag="AGAGCAAGAGCA">AGAGCAAGAGCA</BASECALL>
                        </EXPECTED_BASECALL_TABLE>
                    </READ_SPEC>
                    <READ_SPEC>
                        <READ_INDEX>2</READ_INDEX>
                        <READ_CLASS>Application Read</READ_CLASS>
                        <READ_TYPE>Forward</READ_TYPE>
                        <RELATIVE_ORDER follows_read_index="1"/>
                    </READ_SPEC>
                </SPOT_DECODE_SPEC>
            </SPOT_DESCRIPTOR>
        </DESIGN>
        <PLATFORM>
            <LS454>
                <INSTRUMENT_MODEL>454 GS FLX Titanium</INSTRUMENT_MODEL>
            </LS454>
        </PLATFORM>
        <PROCESSING>
            <PIPELINE>
                <PIPE_SECTION>
                    <STEP_INDEX></STEP_INDEX>
                    <PREV_STEP_INDEX></PREV_STEP_INDEX>
                    <PROGRAM>454 Basecaller v2.3</PROGRAM>
                    <VERSION></VERSION>
                </PIPE_SECTION>
            </PIPELINE>
        </PROCESSING>
    </EXPERIMENT>
</EXPERIMENT_SET>
