PCR bias in nanoCAGE: influence of cycle number and reduction by fingerprinting.
PRJDB2775
1) Barcode (6 bases, read type "BarCode")
2) Fingerprint (8 bases, read type "Other")
3) Linker (7 bases, read type "Linker")
4) CAGE tag (remaining bases, read type "Forward")
SAMD00015951
NChi10019
OTHER
TRANSCRIPTOMIC
PCR
202
0
barcode_tag
Technical Read
BarCode
ACAGAT
ATCGTG
CACGAT
CACTGA
CTGACG
GAGTGA
GTATAC
TCGAGC
1
Technical Read
Other
7
2
Technical Read
Linker
15
3
Application Read
Forward
22
4
Application Read
Reverse
102
Illumina HiSeq 2000
1
NIL
Elimination of non-capped RNA molecules before nanoCAGE library preparation.
PRJDB2775
1) Barcode (6 bases, read type "BarCode")
2) Fingerprint (8 bases, read type "Other")
3) Linker (7 bases, read type "Linker")
4) CAGE tag (remaining bases, read type "Forward")
SAMD00015946
NChi10019
OTHER
TRANSCRIPTOMIC
PCR
202
0
barcode_tag
Technical Read
BarCode
ACAGAT
ATCGTG
CACGAT
CACTGA
CTGACG
GAGTGA
GTATAC
TCGAGC
1
Technical Read
Other
7
2
Technical Read
Linker
15
3
Application Read
Forward
22
4
Application Read
Reverse
102
Illumina HiSeq 2000
1
NIL