PRJDB2031 A cDNA library was constructed from leaves, xylem, male and female floral tissues of two A. sachalinensis trees. After total RNA was extracted from each tissues using a CTAB-based method, each batch of RNA was mixed equal-molarly. cDNA synthesis was carried out using SMART cDNA construction kit (Clontech) and normalized using a cDNA Normalization Kit (Evrogen) by Hokkaido System Science Co., Ltd. , Japan. The library was pyrosequenced by Roch 454 GS FLX Titanium. SAMD00006074 Abies sachalinensis EST FL-cDNA TRANSCRIPTOMIC cDNA Total RNA was used for ds cDNA synthesis using SMART approach. SMART prepared amplified cDNA was then normalized using DSN normalization method. Next, the libraries were prepared 500ng cDNA using the "Titanium Rapid Library Preparation Kit". Single 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX 1 NIL