PRJDB2367 After adaptor trimming with the cutadapt program (http://code.google.com/p/cutadapt/) and discarding reads containing N bases, filter-passed sequence reads from the founder RRLs were divided into 3 groups by their 5???-terminal sequences (both-end HaeIII, both-end MboI, and others). Sequence reads within a group were clustered by the clustering program ???SEED??? (http://manuals.bioinformatics.ucr.edu/home/seed) and consensus sequences of 300 bp (read-pair) were generated. These consisted of forward and reverse 101-bp reads with internal 98 bases of N. Consensus sequences with depth ???10 were used as reference sequences for mapping of read-pairs from each founder. 0 NIL SEED 1.5.1