SAMD00012669 10090 Mus musculus 24 nanoCAGE/CAGEscan libraries were prepared from total RNAs of Fluorescence-activated cell sorting (FACS) sorted parvalbumin-positive neurons ranging from 30-127 ng, where four treatments including two HDACi (TSA and VPA) with their vehicle controls, two time points after each treatment (2h and 2d) were compared. Three biological replicates for each combination of treatment and time point were prepared. In order to sequence multiplex libraries at a time, a six nucleotide barcode system was introduced. Briefly, the barcodes were introduced inside the template-switching primer and incorporated at the 5?-end of the nanoCAGE libraries through reverse transcription. After cDNA synthesis, 24 libraries were pooled into single library and adapted for sequencing with an Illumina Genome analyzer IIx. memberName 1.VPA_2h_01 hori-0027_Sample_0002 readGroupTag 5'SOLX_96nCAGE_02 hori-0027_Sample_0002 proportion hori-0027_Sample_0002 memberName 2.VPA_2h_02 hori-0027_Sample_0003 readGroupTag 5'SOLX_96nCAGE_14 hori-0027_Sample_0003 proportion hori-0027_Sample_0003 memberName 3.VPA_2h_03 hori-0027_Sample_0004 readGroupTag 5'SOLX_96nCAGE_26 hori-0027_Sample_0004 proportion hori-0027_Sample_0004 memberName 4.VEH/vpa_2h_01 hori-0027_Sample_0005 readGroupTag 5'SOLX_96nCAGE_38 hori-0027_Sample_0005 proportion hori-0027_Sample_0005 memberName 5.VEH/vpa_2h_02 hori-0027_Sample_0006 readGroupTag 5'SOLX_96nCAGE_50 hori-0027_Sample_0006 proportion hori-0027_Sample_0006 memberName 6.VEH/vpa_2h_03 hori-0027_Sample_0007 readGroupTag 5'SOLX_96nCAGE_62 hori-0027_Sample_0007 proportion hori-0027_Sample_0007 memberName 7.VPA_2d_01 hori-0027_Sample_0008 readGroupTag 5'SOLX_96nCAGE_74 hori-0027_Sample_0008 proportion hori-0027_Sample_0008 memberName 8.VPA_2d_02 hori-0027_Sample_0009 readGroupTag 5'SOLX_96nCAGE_86 hori-0027_Sample_0009 proportion hori-0027_Sample_0009 memberName 9.VPA_2d_03 hori-0027_Sample_0010 readGroupTag 5'SOLX_96nCAGE_06 hori-0027_Sample_0010 proportion hori-0027_Sample_0010 memberName 10.VEH/vpa_2d_01 hori-0027_Sample_0011 readGroupTag 5'SOLX_96nCAGE_18 hori-0027_Sample_0011 proportion hori-0027_Sample_0011 memberName 11.VEH/vpa_2d_02 hori-0027_Sample_0012 readGroupTag 5'SOLX_96nCAGE_30 hori-0027_Sample_0012 proportion hori-0027_Sample_0012 memberName 12.VEH/vpa_2d_03 hori-0027_Sample_0013 readGroupTag 5'SOLX_96nCAGE_42 hori-0027_Sample_0013 proportion hori-0027_Sample_0013 memberName 13.TSA_2h_01 hori-0027_Sample_0014 readGroupTag 5'SOLX_96nCAGE_54 hori-0027_Sample_0014 proportion hori-0027_Sample_0014 memberName 14.TSA_2h_02 hori-0027_Sample_0015 readGroupTag 5'SOLX_96nCAGE_66 hori-0027_Sample_0015 proportion hori-0027_Sample_0015 memberName 15.TSA_2h_04 hori-0027_Sample_0016 readGroupTag 5'SOLX_96nCAGE_78 hori-0027_Sample_0016 proportion hori-0027_Sample_0016 memberName 16.VEH/tsa_2h_02 hori-0027_Sample_0017 readGroupTag 5'SOLX_96nCAGE_90 hori-0027_Sample_0017 proportion hori-0027_Sample_0017 memberName 17.VEH/tsa_2h_03 hori-0027_Sample_0018 readGroupTag 5'SOLX_96nCAGE_07 hori-0027_Sample_0018 proportion hori-0027_Sample_0018 memberName 18.VEH/tsa_2h_04 hori-0027_Sample_0019 readGroupTag 5'SOLX_96nCAGE_19 hori-0027_Sample_0019 proportion hori-0027_Sample_0019 memberName 19.TSA_2d_01 hori-0027_Sample_0020 readGroupTag 5'SOLX_96nCAGE_31 hori-0027_Sample_0020 proportion hori-0027_Sample_0020 memberName 20.TSA_2d_02 hori-0027_Sample_0021 readGroupTag 5'SOLX_96nCAGE_43 hori-0027_Sample_0021 proportion hori-0027_Sample_0021 memberName 21.TSA_2d_03 hori-0027_Sample_0022 readGroupTag 5'SOLX_96nCAGE_55 hori-0027_Sample_0022 proportion hori-0027_Sample_0022 memberName 22.VEH/tsa_2d_01 hori-0027_Sample_0023 readGroupTag 5'SOLX_96nCAGE_67 hori-0027_Sample_0023 proportion hori-0027_Sample_0023 memberName 23.VEH/tsa_2d_02 hori-0027_Sample_0024 readGroupTag 5'SOLX_96nCAGE_79 hori-0027_Sample_0024 proportion hori-0027_Sample_0024 memberName 24.VEH/tsa_2d_03 hori-0027_Sample_0025 readGroupTag 5'SOLX_96nCAGE_91 hori-0027_Sample_0025 proportion hori-0027_Sample_0025