<?xml version="1.0" encoding="UTF-8" standalone="yes"?>
<EXPERIMENT_SET>
    <EXPERIMENT accession="DRX001906" center_name="FUKUOKA_ARC" alias="DRX001906">
        <TITLE>454 sequencing of cDNA fragment library from Ficus carica (fig) caprifig fruits at period II</TITLE>
        <STUDY_REF accession="DRP000661" refcenter="FUKUOKA_ARC" refname="DRP000661">
            <IDENTIFIERS>
                <PRIMARY_ID label="BioProject ID">PRJDB2258</PRIMARY_ID>
            </IDENTIFIERS>
        </STUDY_REF>
        <DESIGN>
            <DESIGN_DESCRIPTION></DESIGN_DESCRIPTION>
            <SAMPLE_DESCRIPTOR accession="DRS002062" refcenter="FUKUOKA_ARC" refname="DRS002062">
                <IDENTIFIERS>
                    <PRIMARY_ID label="BioSample ID">SAMD00009608</PRIMARY_ID>
                </IDENTIFIERS>
            </SAMPLE_DESCRIPTOR>
            <LIBRARY_DESCRIPTOR>
                <LIBRARY_NAME>FC_caprifig6085_fruits_p2</LIBRARY_NAME>
                <LIBRARY_STRATEGY>EST</LIBRARY_STRATEGY>
                <LIBRARY_SOURCE>TRANSCRIPTOMIC</LIBRARY_SOURCE>
                <LIBRARY_SELECTION>cDNA</LIBRARY_SELECTION>
                <LIBRARY_LAYOUT>
                    <SINGLE/>
                </LIBRARY_LAYOUT>
                <LIBRARY_CONSTRUCTION_PROTOCOL> Two harvested fruits for caprifig type were sliced vertically and preserved at ?80 ?C following snap-freezing with liquid nitrogen.The fruits were ground in liquid nitrogen, and subjected to total RNA extraction, combining Fruits-mate (TakaraBio, Inc., Shiga, Japan) and an RNeasy Maxi kit (Qiagen, Hilden Germany) as a substitute for the NucleoSpin RNA plant?(TakaraBio, Inc.). Purified poly(A)-RNA was then obtained from the total RNA using a MicroPoly(A)Puristkit (Ambion, Carlsbad, CA). We used a cDNA Synthesis System kit (Roche, Germany), Roche ?random primers? (Roche), and aGS FLX Titanium Rapid Library Preparation kit (Roche) to synthesize cDNA and prepare the library, which we then sequenced with aGS FLX Titanium Sequencer (Roche) following the manufacturer?s instructions.</LIBRARY_CONSTRUCTION_PROTOCOL>
            </LIBRARY_DESCRIPTOR>
            <SPOT_DESCRIPTOR>
                <SPOT_DECODE_SPEC>
                    <READ_SPEC>
                        <READ_INDEX>0</READ_INDEX>
                        <READ_LABEL> </READ_LABEL>
                        <READ_CLASS>Technical Read</READ_CLASS>
                        <READ_TYPE>Adapter</READ_TYPE>
                        <BASE_COORD>1</BASE_COORD>
                    </READ_SPEC>
                    <READ_SPEC>
                        <READ_INDEX>1</READ_INDEX>
                        <READ_CLASS>Application Read</READ_CLASS>
                        <READ_TYPE>Forward</READ_TYPE>
                        <BASE_COORD>5</BASE_COORD>
                    </READ_SPEC>
                </SPOT_DECODE_SPEC>
            </SPOT_DESCRIPTOR>
        </DESIGN>
        <PLATFORM>
            <LS454>
                <INSTRUMENT_MODEL>454 GS FLX Titanium</INSTRUMENT_MODEL>
            </LS454>
        </PLATFORM>
        <PROCESSING>
            <PIPELINE>
                <PIPE_SECTION section_name=" primary analysis">
                    <STEP_INDEX>1</STEP_INDEX>
                    <PREV_STEP_INDEX>NIL</PREV_STEP_INDEX>
                    <PROGRAM>454 Basecaller</PROGRAM>
                    <VERSION>1</VERSION>
                </PIPE_SECTION>
            </PIPELINE>
        </PROCESSING>
    </EXPERIMENT>
    <EXPERIMENT accession="DRX001907" center_name="FUKUOKA_ARC" alias="DRX001907">
        <TITLE>454 sequencing of cDNA fragment library from Ficus carica (fig) common fig fruits at period II</TITLE>
        <STUDY_REF accession="DRP000661" refcenter="FUKUOKA_ARC" refname="DRP000661">
            <IDENTIFIERS>
                <PRIMARY_ID label="BioProject ID">PRJDB2258</PRIMARY_ID>
            </IDENTIFIERS>
        </STUDY_REF>
        <DESIGN>
            <DESIGN_DESCRIPTION></DESIGN_DESCRIPTION>
            <SAMPLE_DESCRIPTOR accession="DRS002063" refcenter="FUKUOKA_ARC" refname="DRS002063">
                <IDENTIFIERS>
                    <PRIMARY_ID label="BioSample ID">SAMD00009609</PRIMARY_ID>
                </IDENTIFIERS>
            </SAMPLE_DESCRIPTOR>
            <LIBRARY_DESCRIPTOR>
                <LIBRARY_NAME>FC_Houraishi_fruits_p2</LIBRARY_NAME>
                <LIBRARY_STRATEGY>EST</LIBRARY_STRATEGY>
                <LIBRARY_SOURCE>TRANSCRIPTOMIC</LIBRARY_SOURCE>
                <LIBRARY_SELECTION>cDNA</LIBRARY_SELECTION>
                <LIBRARY_LAYOUT>
                    <SINGLE/>
                </LIBRARY_LAYOUT>
                <LIBRARY_CONSTRUCTION_PROTOCOL> Two harvested fruits for common fig type were sliced vertically and preserved at ?80 ?C following snap-freezing with liquid nitrogen.The fruits were ground in liquid nitrogen, and subjected to total RNA extraction, combining Fruits-mate (TakaraBio, Inc., Shiga, Japan) and an RNeasy Maxi kit (Qiagen, Hilden Germany) as a substitute for the NucleoSpin RNA plant?(TakaraBio, Inc.). Purified poly(A)-RNA was then obtained from the total RNA using a MicroPoly(A)Puristkit (Ambion, Carlsbad, CA). We used a cDNA Synthesis System kit (Roche, Germany), Roche ?random primers? (Roche), and aGS FLX Titanium Rapid Library Preparation kit (Roche) to synthesize cDNA and prepare the library, which we then sequenced with aGS FLX Titanium Sequencer (Roche) following the manufacturer?s instructions.</LIBRARY_CONSTRUCTION_PROTOCOL>
            </LIBRARY_DESCRIPTOR>
            <SPOT_DESCRIPTOR>
                <SPOT_DECODE_SPEC>
                    <READ_SPEC>
                        <READ_INDEX>0</READ_INDEX>
                        <READ_LABEL> </READ_LABEL>
                        <READ_CLASS>Technical Read</READ_CLASS>
                        <READ_TYPE>Adapter</READ_TYPE>
                        <BASE_COORD>1</BASE_COORD>
                    </READ_SPEC>
                    <READ_SPEC>
                        <READ_INDEX>1</READ_INDEX>
                        <READ_CLASS>Application Read</READ_CLASS>
                        <READ_TYPE>Forward</READ_TYPE>
                        <BASE_COORD>5</BASE_COORD>
                    </READ_SPEC>
                </SPOT_DECODE_SPEC>
            </SPOT_DESCRIPTOR>
        </DESIGN>
        <PLATFORM>
            <LS454>
                <INSTRUMENT_MODEL>454 GS FLX Titanium</INSTRUMENT_MODEL>
            </LS454>
        </PLATFORM>
        <PROCESSING>
            <PIPELINE>
                <PIPE_SECTION section_name="primary analysis ">
                    <STEP_INDEX>1</STEP_INDEX>
                    <PREV_STEP_INDEX>NIL</PREV_STEP_INDEX>
                    <PROGRAM>454 Basecaller</PROGRAM>
                    <VERSION>1</VERSION>
                </PIPE_SECTION>
            </PIPELINE>
        </PROCESSING>
    </EXPERIMENT>
</EXPERIMENT_SET>
