PRJDB496 SAMD00015754 WGS GENOMIC RANDOM Genomic DNA of B. subtilis Miyagi-4 was isolated from Luria Broth culture according to a routine biochemical isolation procedure. RNase-treated genomic DNA was used for construction of a paired-end library with a Paired End Sample Prep kit (Illumina, San Diego, CA) according to the manufacturer?s instructions. The constructed libraries were sequenced (56 cycles, paired-end runs) using an Illumina genome analyzer IIx. 112 0 Application Read Forward 1 1 Application Read Reverse 56 Illumina Genome Analyzer IIx 1 NIL