PRJDB712 ChIP-seq of RNA polymerase II After fixation with 1% of formaldehyde and quenching with 125 mM glycine, cells were disrupted by shaking with glass beads (11079-105, BioSpec) in a Multi-Beads Shocker (MB400U, Yasui Kikai, Japan). A Bioruptor (UCD-250, Cosmo-bio, Japan) was used for DNA shearing. For immunoprecipitation, antibody against the C-terminal domain of Pol II (Novus Biologicals, 4H8) was used. QIAquick PCR Purification Kit (28106, QIAGEN) was applied to purify immunoprecipitated DNA. Sequencing was done at BGI (http://www.genomics.cn/en/).Accession of input DNA is DRP000890. ChIP-seq of RNA polymerase II (Dspt6) bioproject PRJDB712 PRJDB712 true pubmed 23851719