PCR amplification of 16S rRNA gene (V1-V2 region) sequence

PCR amplification of 16S rRNA gene (V1-V2 region) sequence PRJDB2570 SAMD00012647 AMPLICON METAGENOMIC PCR PCR amplification of the SSU rRNA gene V1-V2 region using forward primer (5?-CCATCTCATCCCTGCGTGTCTCCGACTCAGNNNNNNNNNNagrgtttg atymtggctcag-3?) containing the 454 primer A, a unique 10-bp barcode sequence for each sample (indicated in N), and reverse primer (5?-CCTATCCCCTGTGTGCCTTGGCAGTCTCAGtgctgcctcccgtaggagt-3?) containing the 454 primer B and reverse primer 338R (tgctgcctcccgtaggagt). 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium 1 NIL PCR amplification of 16S rRNA gene (V1-V2 region) sequence PRJDB2570 SAMD00012642 AMPLICON METAGENOMIC PCR PCR amplification of the SSU rRNA gene V1-V2 region using forward primer (5?-CCATCTCATCCCTGCGTGTCTCCGACTCAGNNNNNNNNNNagrgtttg atymtggctcag-3?) containing the 454 primer A, a unique 10-bp barcode sequence for each sample (indicated in N), and reverse primer (5?-CCTATCCCCTGTGTGCCTTGGCAGTCTCAGtgctgcctcccgtaggagt-3?) containing the 454 primer B and reverse primer 338R (tgctgcctcccgtaggagt). 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium 1 NIL PCR amplification of 16S rRNA gene (V1-V2 region) sequence PRJDB2570 SAMD00012643 AMPLICON METAGENOMIC PCR PCR amplification of the SSU rRNA gene V1-V2 region using forward primer (5?-CCATCTCATCCCTGCGTGTCTCCGACTCAGNNNNNNNNNNagrgtttg atymtggctcag-3?) containing the 454 primer A, a unique 10-bp barcode sequence for each sample (indicated in N), and reverse primer (5?-CCTATCCCCTGTGTGCCTTGGCAGTCTCAGtgctgcctcccgtaggagt-3?) containing the 454 primer B and reverse primer 338R (tgctgcctcccgtaggagt). 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium 1 NIL PCR amplification of 16S rRNA gene (V1-V2 region) sequence PRJDB2570 SAMD00012645 AMPLICON METAGENOMIC PCR PCR amplification of the SSU rRNA gene V1-V2 region using forward primer (5?-CCATCTCATCCCTGCGTGTCTCCGACTCAGNNNNNNNNNNagrgtttg atymtggctcag-3?) containing the 454 primer A, a unique 10-bp barcode sequence for each sample (indicated in N), and reverse primer (5?-CCTATCCCCTGTGTGCCTTGGCAGTCTCAGtgctgcctcccgtaggagt-3?) containing the 454 primer B and reverse primer 338R (tgctgcctcccgtaggagt). 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium 1 NIL PCR amplification of 16S rRNA gene (V1-V2 region) sequence PRJDB2570 SAMD00012646 AMPLICON METAGENOMIC PCR PCR amplification of the SSU rRNA gene V1-V2 region using forward primer (5?-CCATCTCATCCCTGCGTGTCTCCGACTCAGNNNNNNNNNNagrgtttg atymtggctcag-3?) containing the 454 primer A, a unique 10-bp barcode sequence for each sample (indicated in N), and reverse primer (5?-CCTATCCCCTGTGTGCCTTGGCAGTCTCAGtgctgcctcccgtaggagt-3?) containing the 454 primer B and reverse primer 338R (tgctgcctcccgtaggagt). 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium 1 NIL PCR amplification of 16S rRNA gene (V1-V2 region) sequence PRJDB2570 SAMD00012640 AMPLICON METAGENOMIC PCR PCR amplification of the SSU rRNA gene V1-V2 region using forward primer (5?-CCATCTCATCCCTGCGTGTCTCCGACTCAGNNNNNNNNNNagrgtttg atymtggctcag-3?) containing the 454 primer A, a unique 10-bp barcode sequence for each sample (indicated in N), and reverse primer (5?-CCTATCCCCTGTGTGCCTTGGCAGTCTCAGtgctgcctcccgtaggagt-3?) containing the 454 primer B and reverse primer 338R (tgctgcctcccgtaggagt). 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium 1 NIL PCR amplification of 16S rRNA gene (V1-V2 region) sequence PRJDB2570 SAMD00012644 AMPLICON METAGENOMIC PCR PCR amplification of the SSU rRNA gene V1-V2 region using forward primer (5?-CCATCTCATCCCTGCGTGTCTCCGACTCAGNNNNNNNNNNagrgtttg atymtggctcag-3?) containing the 454 primer A, a unique 10-bp barcode sequence for each sample (indicated in N), and reverse primer (5?-CCTATCCCCTGTGTGCCTTGGCAGTCTCAGtgctgcctcccgtaggagt-3?) containing the 454 primer B and reverse primer 338R (tgctgcctcccgtaggagt). 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium 1 NIL PCR amplification of 16S rRNA gene (V1-V2 region) sequence PRJDB2570 SAMD00012639 AMPLICON METAGENOMIC PCR PCR amplification of the SSU rRNA gene V1-V2 region using forward primer (5?-CCATCTCATCCCTGCGTGTCTCCGACTCAGNNNNNNNNNNagrgtttg atymtggctcag-3?) containing the 454 primer A, a unique 10-bp barcode sequence for each sample (indicated in N), and reverse primer (5?-CCTATCCCCTGTGTGCCTTGGCAGTCTCAGtgctgcctcccgtaggagt-3?) containing the 454 primer B and reverse primer 338R (tgctgcctcccgtaggagt). 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium 1 NIL PCR amplification of 16S rRNA gene (V1-V2 region) sequence PRJDB2570 SAMD00012641 AMPLICON METAGENOMIC PCR PCR amplification of the SSU rRNA gene V1-V2 region using forward primer (5?-CCATCTCATCCCTGCGTGTCTCCGACTCAGNNNNNNNNNNagrgtttg atymtggctcag-3?) containing the 454 primer A, a unique 10-bp barcode sequence for each sample (indicated in N), and reverse primer (5?-CCTATCCCCTGTGTGCCTTGGCAGTCTCAGtgctgcctcccgtaggagt-3?) containing the 454 primer B and reverse primer 338R (tgctgcctcccgtaggagt). 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium 1 NIL