PRJDB2323 DNA extraction from individual soil sample (0.10 g dry weight per extraction) by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00010068 HESHY4K01__AACCAACC__ITS AMPLICON METAGENOMIC PCR pubmed 22808280 First PCR: PCR-amplified with the fungus-specific high-coverage primer ITS1-F_KYO2 (5'-TAG AGG AAG TAA AAG TCG TAA-3'; Toju et al. 2012) and the universal primer LR3 (5'-CCG TGT TTC AAG ACG GG-3'; Vilgalys and Gonzalez 1990). The first PCR was performed with the buffer system of Ampdirect Plus (Shimadzu) and BIOTAQ HS DNA Polymerase (Bioline). The PCR condition for the first step was as follows: 95 degrees C for 10 m, followed by 30 cycles of 94 degrees C for 30s, 53 degrees C for 30 s and 72 degrees C for 90 s, and a final extension at 72 degrees C for 7 m. Second PCR: Targeting a 0.5-kb fragment of ITS2 and partial LSU regions with the forward primer ITS3_KYO2 (Toju et al. 2012) fused with the 454 Adaptor A sequence and a multiplex identifier (MID) tag sequence (Hamady et al. 2008) to identify source soil samples (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG [Adaptor A] ?xxxxxxxx [MID] -GAT GAA GAA CGY AGY RAA-3' [ITS3_KYO2]), and the reverse primer LR_KYO1b fused with the 454 Adaptor B sequence (5'-CCT ATC CCC TGT GTG CCT TGG CAG TCT CAG [Adaptor B] -MGC WGC ATT CCC AAA CWA-3' [LR_KYO1b]). The second PCR was conducted with the buffer system of Taq DNA Polymerase with Standard Taq Buffer (New England BioLabs) under a temperature profile of 95 degrees C for 2 m, followed by 40 cycles of 94 degrees C for 30 s, 50 degrees C for 30 s and 72 degrees C for 60 s, and final extension step at 72 degrees C for 7 m. 0 Technical Read Adapter TCAG 1 barcode_tag Technical Read BarCode AACCAACC 2 Technical Read Primer GATGAAGAACGYAGYRAA 3 Application Read Forward 31 454 GS Junior 1 NIL PRJDB2323 DNA extraction from individual soil sample (0.10 g dry weight per extraction) by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00010065 HESHY4K01__AACCAAGG__ITS AMPLICON METAGENOMIC PCR pubmed 22808280 First PCR: PCR-amplified with the fungus-specific high-coverage primer ITS1-F_KYO2 (5'-TAG AGG AAG TAA AAG TCG TAA-3'; Toju et al. 2012) and the universal primer LR3 (5'-CCG TGT TTC AAG ACG GG-3'; Vilgalys and Gonzalez 1990). The first PCR was performed with the buffer system of Ampdirect Plus (Shimadzu) and BIOTAQ HS DNA Polymerase (Bioline). The PCR condition for the first step was as follows: 95 degrees C for 10 m, followed by 30 cycles of 94 degrees C for 30s, 53 degrees C for 30 s and 72 degrees C for 90 s, and a final extension at 72 degrees C for 7 m. Second PCR: Targeting a 0.5-kb fragment of ITS2 and partial LSU regions with the forward primer ITS3_KYO2 (Toju et al. 2012) fused with the 454 Adaptor A sequence and a multiplex identifier (MID) tag sequence (Hamady et al. 2008) to identify source soil samples (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG [Adaptor A] ?xxxxxxxx [MID] -GAT GAA GAA CGY AGY RAA-3' [ITS3_KYO2]), and the reverse primer LR_KYO1b fused with the 454 Adaptor B sequence (5'-CCT ATC CCC TGT GTG CCT TGG CAG TCT CAG [Adaptor B] -MGC WGC ATT CCC AAA CWA-3' [LR_KYO1b]). The second PCR was conducted with the buffer system of Taq DNA Polymerase with Standard Taq Buffer (New England BioLabs) under a temperature profile of 95 degrees C for 2 m, followed by 40 cycles of 94 degrees C for 30 s, 50 degrees C for 30 s and 72 degrees C for 60 s, and final extension step at 72 degrees C for 7 m. 0 Technical Read Adapter TCAG 1 barcode_tag Technical Read BarCode AACCAAGG 2 Technical Read Primer GATGAAGAACGYAGYRAA 3 Application Read Forward 31 454 GS Junior 1 NIL PRJDB2323 DNA extraction from individual soil sample (0.10 g dry weight per extraction) by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00010073 HESHY4K01__AACCATCG__ITS AMPLICON METAGENOMIC PCR pubmed 22808280 First PCR: PCR-amplified with the fungus-specific high-coverage primer ITS1-F_KYO2 (5'-TAG AGG AAG TAA AAG TCG TAA-3'; Toju et al. 2012) and the universal primer LR3 (5'-CCG TGT TTC AAG ACG GG-3'; Vilgalys and Gonzalez 1990). The first PCR was performed with the buffer system of Ampdirect Plus (Shimadzu) and BIOTAQ HS DNA Polymerase (Bioline). The PCR condition for the first step was as follows: 95 degrees C for 10 m, followed by 30 cycles of 94 degrees C for 30s, 53 degrees C for 30 s and 72 degrees C for 90 s, and a final extension at 72 degrees C for 7 m. Second PCR: Targeting a 0.5-kb fragment of ITS2 and partial LSU regions with the forward primer ITS3_KYO2 (Toju et al. 2012) fused with the 454 Adaptor A sequence and a multiplex identifier (MID) tag sequence (Hamady et al. 2008) to identify source soil samples (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG [Adaptor A] ?xxxxxxxx [MID] -GAT GAA GAA CGY AGY RAA-3' [ITS3_KYO2]), and the reverse primer LR_KYO1b fused with the 454 Adaptor B sequence (5'-CCT ATC CCC TGT GTG CCT TGG CAG TCT CAG [Adaptor B] -MGC WGC ATT CCC AAA CWA-3' [LR_KYO1b]). The second PCR was conducted with the buffer system of Taq DNA Polymerase with Standard Taq Buffer (New England BioLabs) under a temperature profile of 95 degrees C for 2 m, followed by 40 cycles of 94 degrees C for 30 s, 50 degrees C for 30 s and 72 degrees C for 60 s, and final extension step at 72 degrees C for 7 m. 0 Technical Read Adapter TCAG 1 barcode_tag Technical Read BarCode AACCATCG 2 Technical Read Primer GATGAAGAACGYAGYRAA 3 Application Read Forward 31 454 GS Junior 1 NIL PRJDB2323 DNA extraction from individual soil sample (0.10 g dry weight per extraction) by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00010069 HESHY4K01__AACCATGC__ITS AMPLICON METAGENOMIC PCR pubmed 22808280 First PCR: PCR-amplified with the fungus-specific high-coverage primer ITS1-F_KYO2 (5'-TAG AGG AAG TAA AAG TCG TAA-3'; Toju et al. 2012) and the universal primer LR3 (5'-CCG TGT TTC AAG ACG GG-3'; Vilgalys and Gonzalez 1990). The first PCR was performed with the buffer system of Ampdirect Plus (Shimadzu) and BIOTAQ HS DNA Polymerase (Bioline). The PCR condition for the first step was as follows: 95 degrees C for 10 m, followed by 30 cycles of 94 degrees C for 30s, 53 degrees C for 30 s and 72 degrees C for 90 s, and a final extension at 72 degrees C for 7 m. Second PCR: Targeting a 0.5-kb fragment of ITS2 and partial LSU regions with the forward primer ITS3_KYO2 (Toju et al. 2012) fused with the 454 Adaptor A sequence and a multiplex identifier (MID) tag sequence (Hamady et al. 2008) to identify source soil samples (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG [Adaptor A] ?xxxxxxxx [MID] -GAT GAA GAA CGY AGY RAA-3' [ITS3_KYO2]), and the reverse primer LR_KYO1b fused with the 454 Adaptor B sequence (5'-CCT ATC CCC TGT GTG CCT TGG CAG TCT CAG [Adaptor B] -MGC WGC ATT CCC AAA CWA-3' [LR_KYO1b]). The second PCR was conducted with the buffer system of Taq DNA Polymerase with Standard Taq Buffer (New England BioLabs) under a temperature profile of 95 degrees C for 2 m, followed by 40 cycles of 94 degrees C for 30 s, 50 degrees C for 30 s and 72 degrees C for 60 s, and final extension step at 72 degrees C for 7 m. 0 Technical Read Adapter TCAG 1 barcode_tag Technical Read BarCode AACCATGC 2 Technical Read Primer GATGAAGAACGYAGYRAA 3 Application Read Forward 31 454 GS Junior 1 NIL PRJDB2323 DNA extraction from individual soil sample (0.10 g dry weight per extraction) by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00010028 HESHY4K01__AACCGCAT__ITS AMPLICON METAGENOMIC PCR pubmed 22808280 First PCR: PCR-amplified with the fungus-specific high-coverage primer ITS1-F_KYO2 (5'-TAG AGG AAG TAA AAG TCG TAA-3'; Toju et al. 2012) and the universal primer LR3 (5'-CCG TGT TTC AAG ACG GG-3'; Vilgalys and Gonzalez 1990). The first PCR was performed with the buffer system of Ampdirect Plus (Shimadzu) and BIOTAQ HS DNA Polymerase (Bioline). The PCR condition for the first step was as follows: 95 degrees C for 10 m, followed by 30 cycles of 94 degrees C for 30s, 53 degrees C for 30 s and 72 degrees C for 90 s, and a final extension at 72 degrees C for 7 m. Second PCR: Targeting a 0.5-kb fragment of ITS2 and partial LSU regions with the forward primer ITS3_KYO2 (Toju et al. 2012) fused with the 454 Adaptor A sequence and a multiplex identifier (MID) tag sequence (Hamady et al. 2008) to identify source soil samples (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG [Adaptor A] ?xxxxxxxx [MID] -GAT GAA GAA CGY AGY RAA-3' [ITS3_KYO2]), and the reverse primer LR_KYO1b fused with the 454 Adaptor B sequence (5'-CCT ATC CCC TGT GTG CCT TGG CAG TCT CAG [Adaptor B] -MGC WGC ATT CCC AAA CWA-3' [LR_KYO1b]). The second PCR was conducted with the buffer system of Taq DNA Polymerase with Standard Taq Buffer (New England BioLabs) under a temperature profile of 95 degrees C for 2 m, followed by 40 cycles of 94 degrees C for 30 s, 50 degrees C for 30 s and 72 degrees C for 60 s, and final extension step at 72 degrees C for 7 m. 0 Technical Read Adapter TCAG 1 barcode_tag Technical Read BarCode AACCGCAT 2 Technical Read Primer GATGAAGAACGYAGYRAA 3 Application Read Forward 31 454 GS Junior 1 NIL PRJDB2323 DNA extraction from individual soil sample (0.10 g dry weight per extraction) by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00010074 HESHY4K01__AACCGCTA__ITS AMPLICON METAGENOMIC PCR pubmed 22808280 First PCR: PCR-amplified with the fungus-specific high-coverage primer ITS1-F_KYO2 (5'-TAG AGG AAG TAA AAG TCG TAA-3'; Toju et al. 2012) and the universal primer LR3 (5'-CCG TGT TTC AAG ACG GG-3'; Vilgalys and Gonzalez 1990). The first PCR was performed with the buffer system of Ampdirect Plus (Shimadzu) and BIOTAQ HS DNA Polymerase (Bioline). The PCR condition for the first step was as follows: 95 degrees C for 10 m, followed by 30 cycles of 94 degrees C for 30s, 53 degrees C for 30 s and 72 degrees C for 90 s, and a final extension at 72 degrees C for 7 m. Second PCR: Targeting a 0.5-kb fragment of ITS2 and partial LSU regions with the forward primer ITS3_KYO2 (Toju et al. 2012) fused with the 454 Adaptor A sequence and a multiplex identifier (MID) tag sequence (Hamady et al. 2008) to identify source soil samples (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG [Adaptor A] ?xxxxxxxx [MID] -GAT GAA GAA CGY AGY RAA-3' [ITS3_KYO2]), and the reverse primer LR_KYO1b fused with the 454 Adaptor B sequence (5'-CCT ATC CCC TGT GTG CCT TGG CAG TCT CAG [Adaptor B] -MGC WGC ATT CCC AAA CWA-3' [LR_KYO1b]). The second PCR was conducted with the buffer system of Taq DNA Polymerase with Standard Taq Buffer (New England BioLabs) under a temperature profile of 95 degrees C for 2 m, followed by 40 cycles of 94 degrees C for 30 s, 50 degrees C for 30 s and 72 degrees C for 60 s, and final extension step at 72 degrees C for 7 m. 0 Technical Read Adapter TCAG 1 barcode_tag Technical Read BarCode AACCGCTA 2 Technical Read Primer GATGAAGAACGYAGYRAA 3 Application Read Forward 31 454 GS Junior 1 NIL PRJDB2323 DNA extraction from individual soil sample (0.10 g dry weight per extraction) by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00010064 HESHY4K01__AACCGGAA__ITS AMPLICON METAGENOMIC PCR pubmed 22808280 First PCR: PCR-amplified with the fungus-specific high-coverage primer ITS1-F_KYO2 (5'-TAG AGG AAG TAA AAG TCG TAA-3'; Toju et al. 2012) and the universal primer LR3 (5'-CCG TGT TTC AAG ACG GG-3'; Vilgalys and Gonzalez 1990). The first PCR was performed with the buffer system of Ampdirect Plus (Shimadzu) and BIOTAQ HS DNA Polymerase (Bioline). The PCR condition for the first step was as follows: 95 degrees C for 10 m, followed by 30 cycles of 94 degrees C for 30s, 53 degrees C for 30 s and 72 degrees C for 90 s, and a final extension at 72 degrees C for 7 m. Second PCR: Targeting a 0.5-kb fragment of ITS2 and partial LSU regions with the forward primer ITS3_KYO2 (Toju et al. 2012) fused with the 454 Adaptor A sequence and a multiplex identifier (MID) tag sequence (Hamady et al. 2008) to identify source soil samples (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG [Adaptor A] ?xxxxxxxx [MID] -GAT GAA GAA CGY AGY RAA-3' [ITS3_KYO2]), and the reverse primer LR_KYO1b fused with the 454 Adaptor B sequence (5'-CCT ATC CCC TGT GTG CCT TGG CAG TCT CAG [Adaptor B] -MGC WGC ATT CCC AAA CWA-3' [LR_KYO1b]). The second PCR was conducted with the buffer system of Taq DNA Polymerase with Standard Taq Buffer (New England BioLabs) under a temperature profile of 95 degrees C for 2 m, followed by 40 cycles of 94 degrees C for 30 s, 50 degrees C for 30 s and 72 degrees C for 60 s, and final extension step at 72 degrees C for 7 m. 0 Technical Read Adapter TCAG 1 barcode_tag Technical Read BarCode AACCGGAA 2 Technical Read Primer GATGAAGAACGYAGYRAA 3 Application Read Forward 31 454 GS Junior 1 NIL PRJDB2323 DNA extraction from individual soil sample (0.10 g dry weight per extraction) by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00010059 HESHY4K01__AACCGGTT__ITS AMPLICON METAGENOMIC PCR pubmed 22808280 First PCR: PCR-amplified with the fungus-specific high-coverage primer ITS1-F_KYO2 (5'-TAG AGG AAG TAA AAG TCG TAA-3'; Toju et al. 2012) and the universal primer LR3 (5'-CCG TGT TTC AAG ACG GG-3'; Vilgalys and Gonzalez 1990). The first PCR was performed with the buffer system of Ampdirect Plus (Shimadzu) and BIOTAQ HS DNA Polymerase (Bioline). The PCR condition for the first step was as follows: 95 degrees C for 10 m, followed by 30 cycles of 94 degrees C for 30s, 53 degrees C for 30 s and 72 degrees C for 90 s, and a final extension at 72 degrees C for 7 m. Second PCR: Targeting a 0.5-kb fragment of ITS2 and partial LSU regions with the forward primer ITS3_KYO2 (Toju et al. 2012) fused with the 454 Adaptor A sequence and a multiplex identifier (MID) tag sequence (Hamady et al. 2008) to identify source soil samples (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG [Adaptor A] ?xxxxxxxx [MID] -GAT GAA GAA CGY AGY RAA-3' [ITS3_KYO2]), and the reverse primer LR_KYO1b fused with the 454 Adaptor B sequence (5'-CCT ATC CCC TGT GTG CCT TGG CAG TCT CAG [Adaptor B] -MGC WGC ATT CCC AAA CWA-3' [LR_KYO1b]). The second PCR was conducted with the buffer system of Taq DNA Polymerase with Standard Taq Buffer (New England BioLabs) under a temperature profile of 95 degrees C for 2 m, followed by 40 cycles of 94 degrees C for 30 s, 50 degrees C for 30 s and 72 degrees C for 60 s, and final extension step at 72 degrees C for 7 m. 0 Technical Read Adapter TCAG 1 barcode_tag Technical Read BarCode AACCGGTT 2 Technical Read Primer GATGAAGAACGYAGYRAA 3 Application Read Forward 31 454 GS Junior 1 NIL PRJDB2323 DNA extraction from individual soil sample (0.10 g dry weight per extraction) by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00010058 HESHY4K01__AACCTACG__ITS AMPLICON METAGENOMIC PCR pubmed 22808280 First PCR: PCR-amplified with the fungus-specific high-coverage primer ITS1-F_KYO2 (5'-TAG AGG AAG TAA AAG TCG TAA-3'; Toju et al. 2012) and the universal primer LR3 (5'-CCG TGT TTC AAG ACG GG-3'; Vilgalys and Gonzalez 1990). The first PCR was performed with the buffer system of Ampdirect Plus (Shimadzu) and BIOTAQ HS DNA Polymerase (Bioline). The PCR condition for the first step was as follows: 95 degrees C for 10 m, followed by 30 cycles of 94 degrees C for 30s, 53 degrees C for 30 s and 72 degrees C for 90 s, and a final extension at 72 degrees C for 7 m. Second PCR: Targeting a 0.5-kb fragment of ITS2 and partial LSU regions with the forward primer ITS3_KYO2 (Toju et al. 2012) fused with the 454 Adaptor A sequence and a multiplex identifier (MID) tag sequence (Hamady et al. 2008) to identify source soil samples (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG [Adaptor A] ?xxxxxxxx [MID] -GAT GAA GAA CGY AGY RAA-3' [ITS3_KYO2]), and the reverse primer LR_KYO1b fused with the 454 Adaptor B sequence (5'-CCT ATC CCC TGT GTG CCT TGG CAG TCT CAG [Adaptor B] -MGC WGC ATT CCC AAA CWA-3' [LR_KYO1b]). The second PCR was conducted with the buffer system of Taq DNA Polymerase with Standard Taq Buffer (New England BioLabs) under a temperature profile of 95 degrees C for 2 m, followed by 40 cycles of 94 degrees C for 30 s, 50 degrees C for 30 s and 72 degrees C for 60 s, and final extension step at 72 degrees C for 7 m. 0 Technical Read Adapter TCAG 1 barcode_tag Technical Read BarCode AACCTACG 2 Technical Read Primer GATGAAGAACGYAGYRAA 3 Application Read Forward 31 454 GS Junior 1 NIL PRJDB2323 DNA extraction from individual soil sample (0.10 g dry weight per extraction) by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00010066 HESHY4K01__AACCTAGC__ITS AMPLICON METAGENOMIC PCR pubmed 22808280 First PCR: PCR-amplified with the fungus-specific high-coverage primer ITS1-F_KYO2 (5'-TAG AGG AAG TAA AAG TCG TAA-3'; Toju et al. 2012) and the universal primer LR3 (5'-CCG TGT TTC AAG ACG GG-3'; Vilgalys and Gonzalez 1990). The first PCR was performed with the buffer system of Ampdirect Plus (Shimadzu) and BIOTAQ HS DNA Polymerase (Bioline). The PCR condition for the first step was as follows: 95 degrees C for 10 m, followed by 30 cycles of 94 degrees C for 30s, 53 degrees C for 30 s and 72 degrees C for 90 s, and a final extension at 72 degrees C for 7 m. Second PCR: Targeting a 0.5-kb fragment of ITS2 and partial LSU regions with the forward primer ITS3_KYO2 (Toju et al. 2012) fused with the 454 Adaptor A sequence and a multiplex identifier (MID) tag sequence (Hamady et al. 2008) to identify source soil samples (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG [Adaptor A] ?xxxxxxxx [MID] -GAT GAA GAA CGY AGY RAA-3' [ITS3_KYO2]), and the reverse primer LR_KYO1b fused with the 454 Adaptor B sequence (5'-CCT ATC CCC TGT GTG CCT TGG CAG TCT CAG [Adaptor B] -MGC WGC ATT CCC AAA CWA-3' [LR_KYO1b]). The second PCR was conducted with the buffer system of Taq DNA Polymerase with Standard Taq Buffer (New England BioLabs) under a temperature profile of 95 degrees C for 2 m, followed by 40 cycles of 94 degrees C for 30 s, 50 degrees C for 30 s and 72 degrees C for 60 s, and final extension step at 72 degrees C for 7 m. 0 Technical Read Adapter TCAG 1 barcode_tag Technical Read BarCode AACCTAGC 2 Technical Read Primer GATGAAGAACGYAGYRAA 3 Application Read Forward 31 454 GS Junior 1 NIL PRJDB2323 DNA extraction from individual soil sample (0.10 g dry weight per extraction) by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00010070 HESHY4K01__AACCTTCC__ITS AMPLICON METAGENOMIC PCR pubmed 22808280 First PCR: PCR-amplified with the fungus-specific high-coverage primer ITS1-F_KYO2 (5'-TAG AGG AAG TAA AAG TCG TAA-3'; Toju et al. 2012) and the universal primer LR3 (5'-CCG TGT TTC AAG ACG GG-3'; Vilgalys and Gonzalez 1990). The first PCR was performed with the buffer system of Ampdirect Plus (Shimadzu) and BIOTAQ HS DNA Polymerase (Bioline). The PCR condition for the first step was as follows: 95 degrees C for 10 m, followed by 30 cycles of 94 degrees C for 30s, 53 degrees C for 30 s and 72 degrees C for 90 s, and a final extension at 72 degrees C for 7 m. Second PCR: Targeting a 0.5-kb fragment of ITS2 and partial LSU regions with the forward primer ITS3_KYO2 (Toju et al. 2012) fused with the 454 Adaptor A sequence and a multiplex identifier (MID) tag sequence (Hamady et al. 2008) to identify source soil samples (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG [Adaptor A] ?xxxxxxxx [MID] -GAT GAA GAA CGY AGY RAA-3' [ITS3_KYO2]), and the reverse primer LR_KYO1b fused with the 454 Adaptor B sequence (5'-CCT ATC CCC TGT GTG CCT TGG CAG TCT CAG [Adaptor B] -MGC WGC ATT CCC AAA CWA-3' [LR_KYO1b]). The second PCR was conducted with the buffer system of Taq DNA Polymerase with Standard Taq Buffer (New England BioLabs) under a temperature profile of 95 degrees C for 2 m, followed by 40 cycles of 94 degrees C for 30 s, 50 degrees C for 30 s and 72 degrees C for 60 s, and final extension step at 72 degrees C for 7 m. 0 Technical Read Adapter TCAG 1 barcode_tag Technical Read BarCode AACCTTCC 2 Technical Read Primer GATGAAGAACGYAGYRAA 3 Application Read Forward 31 454 GS Junior 1 NIL PRJDB2323 DNA extraction from individual soil sample (0.10 g dry weight per extraction) by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00010061 HESHY4K01__AACCTTGG__ITS AMPLICON METAGENOMIC PCR pubmed 22808280 First PCR: PCR-amplified with the fungus-specific high-coverage primer ITS1-F_KYO2 (5'-TAG AGG AAG TAA AAG TCG TAA-3'; Toju et al. 2012) and the universal primer LR3 (5'-CCG TGT TTC AAG ACG GG-3'; Vilgalys and Gonzalez 1990). The first PCR was performed with the buffer system of Ampdirect Plus (Shimadzu) and BIOTAQ HS DNA Polymerase (Bioline). The PCR condition for the first step was as follows: 95 degrees C for 10 m, followed by 30 cycles of 94 degrees C for 30s, 53 degrees C for 30 s and 72 degrees C for 90 s, and a final extension at 72 degrees C for 7 m. Second PCR: Targeting a 0.5-kb fragment of ITS2 and partial LSU regions with the forward primer ITS3_KYO2 (Toju et al. 2012) fused with the 454 Adaptor A sequence and a multiplex identifier (MID) tag sequence (Hamady et al. 2008) to identify source soil samples (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG [Adaptor A] ?xxxxxxxx [MID] -GAT GAA GAA CGY AGY RAA-3' [ITS3_KYO2]), and the reverse primer LR_KYO1b fused with the 454 Adaptor B sequence (5'-CCT ATC CCC TGT GTG CCT TGG CAG TCT CAG [Adaptor B] -MGC WGC ATT CCC AAA CWA-3' [LR_KYO1b]). The second PCR was conducted with the buffer system of Taq DNA Polymerase with Standard Taq Buffer (New England BioLabs) under a temperature profile of 95 degrees C for 2 m, followed by 40 cycles of 94 degrees C for 30 s, 50 degrees C for 30 s and 72 degrees C for 60 s, and final extension step at 72 degrees C for 7 m. 0 Technical Read Adapter TCAG 1 barcode_tag Technical Read BarCode AACCTTGG 2 Technical Read Primer GATGAAGAACGYAGYRAA 3 Application Read Forward 31 454 GS Junior 1 NIL PRJDB2323 DNA extraction from individual soil sample (0.10 g dry weight per extraction) by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00010060 HESHY4K01__AACGAACG__ITS AMPLICON METAGENOMIC PCR pubmed 22808280 First PCR: PCR-amplified with the fungus-specific high-coverage primer ITS1-F_KYO2 (5'-TAG AGG AAG TAA AAG TCG TAA-3'; Toju et al. 2012) and the universal primer LR3 (5'-CCG TGT TTC AAG ACG GG-3'; Vilgalys and Gonzalez 1990). The first PCR was performed with the buffer system of Ampdirect Plus (Shimadzu) and BIOTAQ HS DNA Polymerase (Bioline). The PCR condition for the first step was as follows: 95 degrees C for 10 m, followed by 30 cycles of 94 degrees C for 30s, 53 degrees C for 30 s and 72 degrees C for 90 s, and a final extension at 72 degrees C for 7 m. Second PCR: Targeting a 0.5-kb fragment of ITS2 and partial LSU regions with the forward primer ITS3_KYO2 (Toju et al. 2012) fused with the 454 Adaptor A sequence and a multiplex identifier (MID) tag sequence (Hamady et al. 2008) to identify source soil samples (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG [Adaptor A] ?xxxxxxxx [MID] -GAT GAA GAA CGY AGY RAA-3' [ITS3_KYO2]), and the reverse primer LR_KYO1b fused with the 454 Adaptor B sequence (5'-CCT ATC CCC TGT GTG CCT TGG CAG TCT CAG [Adaptor B] -MGC WGC ATT CCC AAA CWA-3' [LR_KYO1b]). The second PCR was conducted with the buffer system of Taq DNA Polymerase with Standard Taq Buffer (New England BioLabs) under a temperature profile of 95 degrees C for 2 m, followed by 40 cycles of 94 degrees C for 30 s, 50 degrees C for 30 s and 72 degrees C for 60 s, and final extension step at 72 degrees C for 7 m. 0 Technical Read Adapter TCAG 1 barcode_tag Technical Read BarCode AACGAACG 2 Technical Read Primer GATGAAGAACGYAGYRAA 3 Application Read Forward 31 454 GS Junior 1 NIL PRJDB2323 DNA extraction from individual soil sample (0.10 g dry weight per extraction) by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00010046 HESHY4K01__AACGAAGC__ITS AMPLICON METAGENOMIC PCR pubmed 22808280 First PCR: PCR-amplified with the fungus-specific high-coverage primer ITS1-F_KYO2 (5'-TAG AGG AAG TAA AAG TCG TAA-3'; Toju et al. 2012) and the universal primer LR3 (5'-CCG TGT TTC AAG ACG GG-3'; Vilgalys and Gonzalez 1990). The first PCR was performed with the buffer system of Ampdirect Plus (Shimadzu) and BIOTAQ HS DNA Polymerase (Bioline). The PCR condition for the first step was as follows: 95 degrees C for 10 m, followed by 30 cycles of 94 degrees C for 30s, 53 degrees C for 30 s and 72 degrees C for 90 s, and a final extension at 72 degrees C for 7 m. Second PCR: Targeting a 0.5-kb fragment of ITS2 and partial LSU regions with the forward primer ITS3_KYO2 (Toju et al. 2012) fused with the 454 Adaptor A sequence and a multiplex identifier (MID) tag sequence (Hamady et al. 2008) to identify source soil samples (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG [Adaptor A] ?xxxxxxxx [MID] -GAT GAA GAA CGY AGY RAA-3' [ITS3_KYO2]), and the reverse primer LR_KYO1b fused with the 454 Adaptor B sequence (5'-CCT ATC CCC TGT GTG CCT TGG CAG TCT CAG [Adaptor B] -MGC WGC ATT CCC AAA CWA-3' [LR_KYO1b]). The second PCR was conducted with the buffer system of Taq DNA Polymerase with Standard Taq Buffer (New England BioLabs) under a temperature profile of 95 degrees C for 2 m, followed by 40 cycles of 94 degrees C for 30 s, 50 degrees C for 30 s and 72 degrees C for 60 s, and final extension step at 72 degrees C for 7 m. 0 Technical Read Adapter TCAG 1 barcode_tag Technical Read BarCode AACGAAGC 2 Technical Read Primer GATGAAGAACGYAGYRAA 3 Application Read Forward 31 454 GS Junior 1 NIL PRJDB2323 DNA extraction from individual soil sample (0.10 g dry weight per extraction) by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00010034 HESHY4K01__AACGATCC__ITS AMPLICON METAGENOMIC PCR pubmed 22808280 First PCR: PCR-amplified with the fungus-specific high-coverage primer ITS1-F_KYO2 (5'-TAG AGG AAG TAA AAG TCG TAA-3'; Toju et al. 2012) and the universal primer LR3 (5'-CCG TGT TTC AAG ACG GG-3'; Vilgalys and Gonzalez 1990). The first PCR was performed with the buffer system of Ampdirect Plus (Shimadzu) and BIOTAQ HS DNA Polymerase (Bioline). The PCR condition for the first step was as follows: 95 degrees C for 10 m, followed by 30 cycles of 94 degrees C for 30s, 53 degrees C for 30 s and 72 degrees C for 90 s, and a final extension at 72 degrees C for 7 m. Second PCR: Targeting a 0.5-kb fragment of ITS2 and partial LSU regions with the forward primer ITS3_KYO2 (Toju et al. 2012) fused with the 454 Adaptor A sequence and a multiplex identifier (MID) tag sequence (Hamady et al. 2008) to identify source soil samples (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG [Adaptor A] ?xxxxxxxx [MID] -GAT GAA GAA CGY AGY RAA-3' [ITS3_KYO2]), and the reverse primer LR_KYO1b fused with the 454 Adaptor B sequence (5'-CCT ATC CCC TGT GTG CCT TGG CAG TCT CAG [Adaptor B] -MGC WGC ATT CCC AAA CWA-3' [LR_KYO1b]). The second PCR was conducted with the buffer system of Taq DNA Polymerase with Standard Taq Buffer (New England BioLabs) under a temperature profile of 95 degrees C for 2 m, followed by 40 cycles of 94 degrees C for 30 s, 50 degrees C for 30 s and 72 degrees C for 60 s, and final extension step at 72 degrees C for 7 m. 0 Technical Read Adapter TCAG 1 barcode_tag Technical Read BarCode AACGATCC 2 Technical Read Primer GATGAAGAACGYAGYRAA 3 Application Read Forward 31 454 GS Junior 1 NIL PRJDB2323 DNA extraction from individual soil sample (0.10 g dry weight per extraction) by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00010075 HESHY4K01__AACGATGG__ITS AMPLICON METAGENOMIC PCR pubmed 22808280 First PCR: PCR-amplified with the fungus-specific high-coverage primer ITS1-F_KYO2 (5'-TAG AGG AAG TAA AAG TCG TAA-3'; Toju et al. 2012) and the universal primer LR3 (5'-CCG TGT TTC AAG ACG GG-3'; Vilgalys and Gonzalez 1990). The first PCR was performed with the buffer system of Ampdirect Plus (Shimadzu) and BIOTAQ HS DNA Polymerase (Bioline). The PCR condition for the first step was as follows: 95 degrees C for 10 m, followed by 30 cycles of 94 degrees C for 30s, 53 degrees C for 30 s and 72 degrees C for 90 s, and a final extension at 72 degrees C for 7 m. Second PCR: Targeting a 0.5-kb fragment of ITS2 and partial LSU regions with the forward primer ITS3_KYO2 (Toju et al. 2012) fused with the 454 Adaptor A sequence and a multiplex identifier (MID) tag sequence (Hamady et al. 2008) to identify source soil samples (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG [Adaptor A] ?xxxxxxxx [MID] -GAT GAA GAA CGY AGY RAA-3' [ITS3_KYO2]), and the reverse primer LR_KYO1b fused with the 454 Adaptor B sequence (5'-CCT ATC CCC TGT GTG CCT TGG CAG TCT CAG [Adaptor B] -MGC WGC ATT CCC AAA CWA-3' [LR_KYO1b]). The second PCR was conducted with the buffer system of Taq DNA Polymerase with Standard Taq Buffer (New England BioLabs) under a temperature profile of 95 degrees C for 2 m, followed by 40 cycles of 94 degrees C for 30 s, 50 degrees C for 30 s and 72 degrees C for 60 s, and final extension step at 72 degrees C for 7 m. 0 Technical Read Adapter TCAG 1 barcode_tag Technical Read BarCode AACGATGG 2 Technical Read Primer GATGAAGAACGYAGYRAA 3 Application Read Forward 31 454 GS Junior 1 NIL PRJDB2323 DNA extraction from individual soil sample (0.10 g dry weight per extraction) by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00010030 HESHY4K01__AACGCCAT__ITS AMPLICON METAGENOMIC PCR pubmed 22808280 First PCR: PCR-amplified with the fungus-specific high-coverage primer ITS1-F_KYO2 (5'-TAG AGG AAG TAA AAG TCG TAA-3'; Toju et al. 2012) and the universal primer LR3 (5'-CCG TGT TTC AAG ACG GG-3'; Vilgalys and Gonzalez 1990). The first PCR was performed with the buffer system of Ampdirect Plus (Shimadzu) and BIOTAQ HS DNA Polymerase (Bioline). The PCR condition for the first step was as follows: 95 degrees C for 10 m, followed by 30 cycles of 94 degrees C for 30s, 53 degrees C for 30 s and 72 degrees C for 90 s, and a final extension at 72 degrees C for 7 m. Second PCR: Targeting a 0.5-kb fragment of ITS2 and partial LSU regions with the forward primer ITS3_KYO2 (Toju et al. 2012) fused with the 454 Adaptor A sequence and a multiplex identifier (MID) tag sequence (Hamady et al. 2008) to identify source soil samples (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG [Adaptor A] ?xxxxxxxx [MID] -GAT GAA GAA CGY AGY RAA-3' [ITS3_KYO2]), and the reverse primer LR_KYO1b fused with the 454 Adaptor B sequence (5'-CCT ATC CCC TGT GTG CCT TGG CAG TCT CAG [Adaptor B] -MGC WGC ATT CCC AAA CWA-3' [LR_KYO1b]). The second PCR was conducted with the buffer system of Taq DNA Polymerase with Standard Taq Buffer (New England BioLabs) under a temperature profile of 95 degrees C for 2 m, followed by 40 cycles of 94 degrees C for 30 s, 50 degrees C for 30 s and 72 degrees C for 60 s, and final extension step at 72 degrees C for 7 m. 0 Technical Read Adapter TCAG 1 barcode_tag Technical Read BarCode AACGCCAT 2 Technical Read Primer GATGAAGAACGYAGYRAA 3 Application Read Forward 31 454 GS Junior 1 NIL PRJDB2323 DNA extraction from individual soil sample (0.10 g dry weight per extraction) by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00010042 HESHY4K01__AACGCCTA__ITS AMPLICON METAGENOMIC PCR pubmed 22808280 First PCR: PCR-amplified with the fungus-specific high-coverage primer ITS1-F_KYO2 (5'-TAG AGG AAG TAA AAG TCG TAA-3'; Toju et al. 2012) and the universal primer LR3 (5'-CCG TGT TTC AAG ACG GG-3'; Vilgalys and Gonzalez 1990). The first PCR was performed with the buffer system of Ampdirect Plus (Shimadzu) and BIOTAQ HS DNA Polymerase (Bioline). The PCR condition for the first step was as follows: 95 degrees C for 10 m, followed by 30 cycles of 94 degrees C for 30s, 53 degrees C for 30 s and 72 degrees C for 90 s, and a final extension at 72 degrees C for 7 m. Second PCR: Targeting a 0.5-kb fragment of ITS2 and partial LSU regions with the forward primer ITS3_KYO2 (Toju et al. 2012) fused with the 454 Adaptor A sequence and a multiplex identifier (MID) tag sequence (Hamady et al. 2008) to identify source soil samples (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG [Adaptor A] ?xxxxxxxx [MID] -GAT GAA GAA CGY AGY RAA-3' [ITS3_KYO2]), and the reverse primer LR_KYO1b fused with the 454 Adaptor B sequence (5'-CCT ATC CCC TGT GTG CCT TGG CAG TCT CAG [Adaptor B] -MGC WGC ATT CCC AAA CWA-3' [LR_KYO1b]). The second PCR was conducted with the buffer system of Taq DNA Polymerase with Standard Taq Buffer (New England BioLabs) under a temperature profile of 95 degrees C for 2 m, followed by 40 cycles of 94 degrees C for 30 s, 50 degrees C for 30 s and 72 degrees C for 60 s, and final extension step at 72 degrees C for 7 m. 0 Technical Read Adapter TCAG 1 barcode_tag Technical Read BarCode AACGCCTA 2 Technical Read Primer GATGAAGAACGYAGYRAA 3 Application Read Forward 31 454 GS Junior 1 NIL PRJDB2323 DNA extraction from individual soil sample (0.10 g dry weight per extraction) by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00010035 HESHY4K01__AACGCGAA__ITS AMPLICON METAGENOMIC PCR pubmed 22808280 First PCR: PCR-amplified with the fungus-specific high-coverage primer ITS1-F_KYO2 (5'-TAG AGG AAG TAA AAG TCG TAA-3'; Toju et al. 2012) and the universal primer LR3 (5'-CCG TGT TTC AAG ACG GG-3'; Vilgalys and Gonzalez 1990). The first PCR was performed with the buffer system of Ampdirect Plus (Shimadzu) and BIOTAQ HS DNA Polymerase (Bioline). The PCR condition for the first step was as follows: 95 degrees C for 10 m, followed by 30 cycles of 94 degrees C for 30s, 53 degrees C for 30 s and 72 degrees C for 90 s, and a final extension at 72 degrees C for 7 m. Second PCR: Targeting a 0.5-kb fragment of ITS2 and partial LSU regions with the forward primer ITS3_KYO2 (Toju et al. 2012) fused with the 454 Adaptor A sequence and a multiplex identifier (MID) tag sequence (Hamady et al. 2008) to identify source soil samples (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG [Adaptor A] ?xxxxxxxx [MID] -GAT GAA GAA CGY AGY RAA-3' [ITS3_KYO2]), and the reverse primer LR_KYO1b fused with the 454 Adaptor B sequence (5'-CCT ATC CCC TGT GTG CCT TGG CAG TCT CAG [Adaptor B] -MGC WGC ATT CCC AAA CWA-3' [LR_KYO1b]). The second PCR was conducted with the buffer system of Taq DNA Polymerase with Standard Taq Buffer (New England BioLabs) under a temperature profile of 95 degrees C for 2 m, followed by 40 cycles of 94 degrees C for 30 s, 50 degrees C for 30 s and 72 degrees C for 60 s, and final extension step at 72 degrees C for 7 m. 0 Technical Read Adapter TCAG 1 barcode_tag Technical Read BarCode AACGCGAA 2 Technical Read Primer GATGAAGAACGYAGYRAA 3 Application Read Forward 31 454 GS Junior 1 NIL PRJDB2323 DNA extraction from individual soil sample (0.10 g dry weight per extraction) by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00010063 HESHY4K01__AACGCGTT__ITS AMPLICON METAGENOMIC PCR pubmed 22808280 First PCR: PCR-amplified with the fungus-specific high-coverage primer ITS1-F_KYO2 (5'-TAG AGG AAG TAA AAG TCG TAA-3'; Toju et al. 2012) and the universal primer LR3 (5'-CCG TGT TTC AAG ACG GG-3'; Vilgalys and Gonzalez 1990). The first PCR was performed with the buffer system of Ampdirect Plus (Shimadzu) and BIOTAQ HS DNA Polymerase (Bioline). The PCR condition for the first step was as follows: 95 degrees C for 10 m, followed by 30 cycles of 94 degrees C for 30s, 53 degrees C for 30 s and 72 degrees C for 90 s, and a final extension at 72 degrees C for 7 m. Second PCR: Targeting a 0.5-kb fragment of ITS2 and partial LSU regions with the forward primer ITS3_KYO2 (Toju et al. 2012) fused with the 454 Adaptor A sequence and a multiplex identifier (MID) tag sequence (Hamady et al. 2008) to identify source soil samples (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG [Adaptor A] ?xxxxxxxx [MID] -GAT GAA GAA CGY AGY RAA-3' [ITS3_KYO2]), and the reverse primer LR_KYO1b fused with the 454 Adaptor B sequence (5'-CCT ATC CCC TGT GTG CCT TGG CAG TCT CAG [Adaptor B] -MGC WGC ATT CCC AAA CWA-3' [LR_KYO1b]). The second PCR was conducted with the buffer system of Taq DNA Polymerase with Standard Taq Buffer (New England BioLabs) under a temperature profile of 95 degrees C for 2 m, followed by 40 cycles of 94 degrees C for 30 s, 50 degrees C for 30 s and 72 degrees C for 60 s, and final extension step at 72 degrees C for 7 m. 0 Technical Read Adapter TCAG 1 barcode_tag Technical Read BarCode AACGCGTT 2 Technical Read Primer GATGAAGAACGYAGYRAA 3 Application Read Forward 31 454 GS Junior 1 NIL PRJDB2323 DNA extraction from individual soil sample (0.10 g dry weight per extraction) by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00010053 HESHY4K01__AACGGCAA__ITS AMPLICON METAGENOMIC PCR pubmed 22808280 First PCR: PCR-amplified with the fungus-specific high-coverage primer ITS1-F_KYO2 (5'-TAG AGG AAG TAA AAG TCG TAA-3'; Toju et al. 2012) and the universal primer LR3 (5'-CCG TGT TTC AAG ACG GG-3'; Vilgalys and Gonzalez 1990). The first PCR was performed with the buffer system of Ampdirect Plus (Shimadzu) and BIOTAQ HS DNA Polymerase (Bioline). The PCR condition for the first step was as follows: 95 degrees C for 10 m, followed by 30 cycles of 94 degrees C for 30s, 53 degrees C for 30 s and 72 degrees C for 90 s, and a final extension at 72 degrees C for 7 m. Second PCR: Targeting a 0.5-kb fragment of ITS2 and partial LSU regions with the forward primer ITS3_KYO2 (Toju et al. 2012) fused with the 454 Adaptor A sequence and a multiplex identifier (MID) tag sequence (Hamady et al. 2008) to identify source soil samples (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG [Adaptor A] ?xxxxxxxx [MID] -GAT GAA GAA CGY AGY RAA-3' [ITS3_KYO2]), and the reverse primer LR_KYO1b fused with the 454 Adaptor B sequence (5'-CCT ATC CCC TGT GTG CCT TGG CAG TCT CAG [Adaptor B] -MGC WGC ATT CCC AAA CWA-3' [LR_KYO1b]). The second PCR was conducted with the buffer system of Taq DNA Polymerase with Standard Taq Buffer (New England BioLabs) under a temperature profile of 95 degrees C for 2 m, followed by 40 cycles of 94 degrees C for 30 s, 50 degrees C for 30 s and 72 degrees C for 60 s, and final extension step at 72 degrees C for 7 m. 0 Technical Read Adapter TCAG 1 barcode_tag Technical Read BarCode AACGGCAA 2 Technical Read Primer GATGAAGAACGYAGYRAA 3 Application Read Forward 31 454 GS Junior 1 NIL PRJDB2323 DNA extraction from individual soil sample (0.10 g dry weight per extraction) by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00010049 HESHY4K01__AACGGCTT__ITS AMPLICON METAGENOMIC PCR pubmed 22808280 First PCR: PCR-amplified with the fungus-specific high-coverage primer ITS1-F_KYO2 (5'-TAG AGG AAG TAA AAG TCG TAA-3'; Toju et al. 2012) and the universal primer LR3 (5'-CCG TGT TTC AAG ACG GG-3'; Vilgalys and Gonzalez 1990). The first PCR was performed with the buffer system of Ampdirect Plus (Shimadzu) and BIOTAQ HS DNA Polymerase (Bioline). The PCR condition for the first step was as follows: 95 degrees C for 10 m, followed by 30 cycles of 94 degrees C for 30s, 53 degrees C for 30 s and 72 degrees C for 90 s, and a final extension at 72 degrees C for 7 m. Second PCR: Targeting a 0.5-kb fragment of ITS2 and partial LSU regions with the forward primer ITS3_KYO2 (Toju et al. 2012) fused with the 454 Adaptor A sequence and a multiplex identifier (MID) tag sequence (Hamady et al. 2008) to identify source soil samples (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG [Adaptor A] ?xxxxxxxx [MID] -GAT GAA GAA CGY AGY RAA-3' [ITS3_KYO2]), and the reverse primer LR_KYO1b fused with the 454 Adaptor B sequence (5'-CCT ATC CCC TGT GTG CCT TGG CAG TCT CAG [Adaptor B] -MGC WGC ATT CCC AAA CWA-3' [LR_KYO1b]). The second PCR was conducted with the buffer system of Taq DNA Polymerase with Standard Taq Buffer (New England BioLabs) under a temperature profile of 95 degrees C for 2 m, followed by 40 cycles of 94 degrees C for 30 s, 50 degrees C for 30 s and 72 degrees C for 60 s, and final extension step at 72 degrees C for 7 m. 0 Technical Read Adapter TCAG 1 barcode_tag Technical Read BarCode AACGGCTT 2 Technical Read Primer GATGAAGAACGYAGYRAA 3 Application Read Forward 31 454 GS Junior 1 NIL PRJDB2323 DNA extraction from individual soil sample (0.10 g dry weight per extraction) by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00010048 HESHY4K01__AACGTACC__ITS AMPLICON METAGENOMIC PCR pubmed 22808280 First PCR: PCR-amplified with the fungus-specific high-coverage primer ITS1-F_KYO2 (5'-TAG AGG AAG TAA AAG TCG TAA-3'; Toju et al. 2012) and the universal primer LR3 (5'-CCG TGT TTC AAG ACG GG-3'; Vilgalys and Gonzalez 1990). The first PCR was performed with the buffer system of Ampdirect Plus (Shimadzu) and BIOTAQ HS DNA Polymerase (Bioline). The PCR condition for the first step was as follows: 95 degrees C for 10 m, followed by 30 cycles of 94 degrees C for 30s, 53 degrees C for 30 s and 72 degrees C for 90 s, and a final extension at 72 degrees C for 7 m. Second PCR: Targeting a 0.5-kb fragment of ITS2 and partial LSU regions with the forward primer ITS3_KYO2 (Toju et al. 2012) fused with the 454 Adaptor A sequence and a multiplex identifier (MID) tag sequence (Hamady et al. 2008) to identify source soil samples (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG [Adaptor A] ?xxxxxxxx [MID] -GAT GAA GAA CGY AGY RAA-3' [ITS3_KYO2]), and the reverse primer LR_KYO1b fused with the 454 Adaptor B sequence (5'-CCT ATC CCC TGT GTG CCT TGG CAG TCT CAG [Adaptor B] -MGC WGC ATT CCC AAA CWA-3' [LR_KYO1b]). The second PCR was conducted with the buffer system of Taq DNA Polymerase with Standard Taq Buffer (New England BioLabs) under a temperature profile of 95 degrees C for 2 m, followed by 40 cycles of 94 degrees C for 30 s, 50 degrees C for 30 s and 72 degrees C for 60 s, and final extension step at 72 degrees C for 7 m. 0 Technical Read Adapter TCAG 1 barcode_tag Technical Read BarCode AACGTACC 2 Technical Read Primer GATGAAGAACGYAGYRAA 3 Application Read Forward 31 454 GS Junior 1 NIL PRJDB2323 DNA extraction from individual soil sample (0.10 g dry weight per extraction) by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00010050 HESHY4K01__AACGTAGG__ITS AMPLICON METAGENOMIC PCR pubmed 22808280 First PCR: PCR-amplified with the fungus-specific high-coverage primer ITS1-F_KYO2 (5'-TAG AGG AAG TAA AAG TCG TAA-3'; Toju et al. 2012) and the universal primer LR3 (5'-CCG TGT TTC AAG ACG GG-3'; Vilgalys and Gonzalez 1990). The first PCR was performed with the buffer system of Ampdirect Plus (Shimadzu) and BIOTAQ HS DNA Polymerase (Bioline). The PCR condition for the first step was as follows: 95 degrees C for 10 m, followed by 30 cycles of 94 degrees C for 30s, 53 degrees C for 30 s and 72 degrees C for 90 s, and a final extension at 72 degrees C for 7 m. Second PCR: Targeting a 0.5-kb fragment of ITS2 and partial LSU regions with the forward primer ITS3_KYO2 (Toju et al. 2012) fused with the 454 Adaptor A sequence and a multiplex identifier (MID) tag sequence (Hamady et al. 2008) to identify source soil samples (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG [Adaptor A] ?xxxxxxxx [MID] -GAT GAA GAA CGY AGY RAA-3' [ITS3_KYO2]), and the reverse primer LR_KYO1b fused with the 454 Adaptor B sequence (5'-CCT ATC CCC TGT GTG CCT TGG CAG TCT CAG [Adaptor B] -MGC WGC ATT CCC AAA CWA-3' [LR_KYO1b]). The second PCR was conducted with the buffer system of Taq DNA Polymerase with Standard Taq Buffer (New England BioLabs) under a temperature profile of 95 degrees C for 2 m, followed by 40 cycles of 94 degrees C for 30 s, 50 degrees C for 30 s and 72 degrees C for 60 s, and final extension step at 72 degrees C for 7 m. 0 Technical Read Adapter TCAG 1 barcode_tag Technical Read BarCode AACGTAGG 2 Technical Read Primer GATGAAGAACGYAGYRAA 3 Application Read Forward 31 454 GS Junior 1 NIL PRJDB2323 DNA extraction from individual soil sample (0.10 g dry weight per extraction) by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00010031 HESHY4K01__AACGTTCG__ITS AMPLICON METAGENOMIC PCR pubmed 22808280 First PCR: PCR-amplified with the fungus-specific high-coverage primer ITS1-F_KYO2 (5'-TAG AGG AAG TAA AAG TCG TAA-3'; Toju et al. 2012) and the universal primer LR3 (5'-CCG TGT TTC AAG ACG GG-3'; Vilgalys and Gonzalez 1990). The first PCR was performed with the buffer system of Ampdirect Plus (Shimadzu) and BIOTAQ HS DNA Polymerase (Bioline). The PCR condition for the first step was as follows: 95 degrees C for 10 m, followed by 30 cycles of 94 degrees C for 30s, 53 degrees C for 30 s and 72 degrees C for 90 s, and a final extension at 72 degrees C for 7 m. Second PCR: Targeting a 0.5-kb fragment of ITS2 and partial LSU regions with the forward primer ITS3_KYO2 (Toju et al. 2012) fused with the 454 Adaptor A sequence and a multiplex identifier (MID) tag sequence (Hamady et al. 2008) to identify source soil samples (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG [Adaptor A] ?xxxxxxxx [MID] -GAT GAA GAA CGY AGY RAA-3' [ITS3_KYO2]), and the reverse primer LR_KYO1b fused with the 454 Adaptor B sequence (5'-CCT ATC CCC TGT GTG CCT TGG CAG TCT CAG [Adaptor B] -MGC WGC ATT CCC AAA CWA-3' [LR_KYO1b]). The second PCR was conducted with the buffer system of Taq DNA Polymerase with Standard Taq Buffer (New England BioLabs) under a temperature profile of 95 degrees C for 2 m, followed by 40 cycles of 94 degrees C for 30 s, 50 degrees C for 30 s and 72 degrees C for 60 s, and final extension step at 72 degrees C for 7 m. 0 Technical Read Adapter TCAG 1 barcode_tag Technical Read BarCode AACGTTCG 2 Technical Read Primer GATGAAGAACGYAGYRAA 3 Application Read Forward 31 454 GS Junior 1 NIL PRJDB2323 DNA extraction from individual soil sample (0.10 g dry weight per extraction) by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00010052 HESHY4K01__AACGTTGC__ITS AMPLICON METAGENOMIC PCR pubmed 22808280 First PCR: PCR-amplified with the fungus-specific high-coverage primer ITS1-F_KYO2 (5'-TAG AGG AAG TAA AAG TCG TAA-3'; Toju et al. 2012) and the universal primer LR3 (5'-CCG TGT TTC AAG ACG GG-3'; Vilgalys and Gonzalez 1990). The first PCR was performed with the buffer system of Ampdirect Plus (Shimadzu) and BIOTAQ HS DNA Polymerase (Bioline). The PCR condition for the first step was as follows: 95 degrees C for 10 m, followed by 30 cycles of 94 degrees C for 30s, 53 degrees C for 30 s and 72 degrees C for 90 s, and a final extension at 72 degrees C for 7 m. Second PCR: Targeting a 0.5-kb fragment of ITS2 and partial LSU regions with the forward primer ITS3_KYO2 (Toju et al. 2012) fused with the 454 Adaptor A sequence and a multiplex identifier (MID) tag sequence (Hamady et al. 2008) to identify source soil samples (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG [Adaptor A] ?xxxxxxxx [MID] -GAT GAA GAA CGY AGY RAA-3' [ITS3_KYO2]), and the reverse primer LR_KYO1b fused with the 454 Adaptor B sequence (5'-CCT ATC CCC TGT GTG CCT TGG CAG TCT CAG [Adaptor B] -MGC WGC ATT CCC AAA CWA-3' [LR_KYO1b]). The second PCR was conducted with the buffer system of Taq DNA Polymerase with Standard Taq Buffer (New England BioLabs) under a temperature profile of 95 degrees C for 2 m, followed by 40 cycles of 94 degrees C for 30 s, 50 degrees C for 30 s and 72 degrees C for 60 s, and final extension step at 72 degrees C for 7 m. 0 Technical Read Adapter TCAG 1 barcode_tag Technical Read BarCode AACGTTGC 2 Technical Read Primer GATGAAGAACGYAGYRAA 3 Application Read Forward 31 454 GS Junior 1 NIL PRJDB2323 DNA extraction from individual soil sample (0.10 g dry weight per extraction) by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00010051 HESHY4K01__AAGCAACG__ITS AMPLICON METAGENOMIC PCR pubmed 22808280 First PCR: PCR-amplified with the fungus-specific high-coverage primer ITS1-F_KYO2 (5'-TAG AGG AAG TAA AAG TCG TAA-3'; Toju et al. 2012) and the universal primer LR3 (5'-CCG TGT TTC AAG ACG GG-3'; Vilgalys and Gonzalez 1990). The first PCR was performed with the buffer system of Ampdirect Plus (Shimadzu) and BIOTAQ HS DNA Polymerase (Bioline). The PCR condition for the first step was as follows: 95 degrees C for 10 m, followed by 30 cycles of 94 degrees C for 30s, 53 degrees C for 30 s and 72 degrees C for 90 s, and a final extension at 72 degrees C for 7 m. Second PCR: Targeting a 0.5-kb fragment of ITS2 and partial LSU regions with the forward primer ITS3_KYO2 (Toju et al. 2012) fused with the 454 Adaptor A sequence and a multiplex identifier (MID) tag sequence (Hamady et al. 2008) to identify source soil samples (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG [Adaptor A] ?xxxxxxxx [MID] -GAT GAA GAA CGY AGY RAA-3' [ITS3_KYO2]), and the reverse primer LR_KYO1b fused with the 454 Adaptor B sequence (5'-CCT ATC CCC TGT GTG CCT TGG CAG TCT CAG [Adaptor B] -MGC WGC ATT CCC AAA CWA-3' [LR_KYO1b]). The second PCR was conducted with the buffer system of Taq DNA Polymerase with Standard Taq Buffer (New England BioLabs) under a temperature profile of 95 degrees C for 2 m, followed by 40 cycles of 94 degrees C for 30 s, 50 degrees C for 30 s and 72 degrees C for 60 s, and final extension step at 72 degrees C for 7 m. 0 Technical Read Adapter TCAG 1 barcode_tag Technical Read BarCode AAGCAACG 2 Technical Read Primer GATGAAGAACGYAGYRAA 3 Application Read Forward 31 454 GS Junior 1 NIL PRJDB2323 DNA extraction from individual soil sample (0.10 g dry weight per extraction) by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00010029 HESHY4K01__AAGCAAGC__ITS AMPLICON METAGENOMIC PCR pubmed 22808280 First PCR: PCR-amplified with the fungus-specific high-coverage primer ITS1-F_KYO2 (5'-TAG AGG AAG TAA AAG TCG TAA-3'; Toju et al. 2012) and the universal primer LR3 (5'-CCG TGT TTC AAG ACG GG-3'; Vilgalys and Gonzalez 1990). The first PCR was performed with the buffer system of Ampdirect Plus (Shimadzu) and BIOTAQ HS DNA Polymerase (Bioline). The PCR condition for the first step was as follows: 95 degrees C for 10 m, followed by 30 cycles of 94 degrees C for 30s, 53 degrees C for 30 s and 72 degrees C for 90 s, and a final extension at 72 degrees C for 7 m. Second PCR: Targeting a 0.5-kb fragment of ITS2 and partial LSU regions with the forward primer ITS3_KYO2 (Toju et al. 2012) fused with the 454 Adaptor A sequence and a multiplex identifier (MID) tag sequence (Hamady et al. 2008) to identify source soil samples (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG [Adaptor A] ?xxxxxxxx [MID] -GAT GAA GAA CGY AGY RAA-3' [ITS3_KYO2]), and the reverse primer LR_KYO1b fused with the 454 Adaptor B sequence (5'-CCT ATC CCC TGT GTG CCT TGG CAG TCT CAG [Adaptor B] -MGC WGC ATT CCC AAA CWA-3' [LR_KYO1b]). The second PCR was conducted with the buffer system of Taq DNA Polymerase with Standard Taq Buffer (New England BioLabs) under a temperature profile of 95 degrees C for 2 m, followed by 40 cycles of 94 degrees C for 30 s, 50 degrees C for 30 s and 72 degrees C for 60 s, and final extension step at 72 degrees C for 7 m. 0 Technical Read Adapter TCAG 1 barcode_tag Technical Read BarCode AAGCAAGC 2 Technical Read Primer GATGAAGAACGYAGYRAA 3 Application Read Forward 31 454 GS Junior 1 NIL PRJDB2323 DNA extraction from individual soil sample (0.10 g dry weight per extraction) by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00010038 HESHY4K01__AAGCATCC__ITS AMPLICON METAGENOMIC PCR pubmed 22808280 First PCR: PCR-amplified with the fungus-specific high-coverage primer ITS1-F_KYO2 (5'-TAG AGG AAG TAA AAG TCG TAA-3'; Toju et al. 2012) and the universal primer LR3 (5'-CCG TGT TTC AAG ACG GG-3'; Vilgalys and Gonzalez 1990). The first PCR was performed with the buffer system of Ampdirect Plus (Shimadzu) and BIOTAQ HS DNA Polymerase (Bioline). The PCR condition for the first step was as follows: 95 degrees C for 10 m, followed by 30 cycles of 94 degrees C for 30s, 53 degrees C for 30 s and 72 degrees C for 90 s, and a final extension at 72 degrees C for 7 m. Second PCR: Targeting a 0.5-kb fragment of ITS2 and partial LSU regions with the forward primer ITS3_KYO2 (Toju et al. 2012) fused with the 454 Adaptor A sequence and a multiplex identifier (MID) tag sequence (Hamady et al. 2008) to identify source soil samples (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG [Adaptor A] ?xxxxxxxx [MID] -GAT GAA GAA CGY AGY RAA-3' [ITS3_KYO2]), and the reverse primer LR_KYO1b fused with the 454 Adaptor B sequence (5'-CCT ATC CCC TGT GTG CCT TGG CAG TCT CAG [Adaptor B] -MGC WGC ATT CCC AAA CWA-3' [LR_KYO1b]). The second PCR was conducted with the buffer system of Taq DNA Polymerase with Standard Taq Buffer (New England BioLabs) under a temperature profile of 95 degrees C for 2 m, followed by 40 cycles of 94 degrees C for 30 s, 50 degrees C for 30 s and 72 degrees C for 60 s, and final extension step at 72 degrees C for 7 m. 0 Technical Read Adapter TCAG 1 barcode_tag Technical Read BarCode AAGCATCC 2 Technical Read Primer GATGAAGAACGYAGYRAA 3 Application Read Forward 31 454 GS Junior 1 NIL PRJDB2323 DNA extraction from individual soil sample (0.10 g dry weight per extraction) by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00010032 HESHY4K01__AAGCATGG__ITS AMPLICON METAGENOMIC PCR pubmed 22808280 First PCR: PCR-amplified with the fungus-specific high-coverage primer ITS1-F_KYO2 (5'-TAG AGG AAG TAA AAG TCG TAA-3'; Toju et al. 2012) and the universal primer LR3 (5'-CCG TGT TTC AAG ACG GG-3'; Vilgalys and Gonzalez 1990). The first PCR was performed with the buffer system of Ampdirect Plus (Shimadzu) and BIOTAQ HS DNA Polymerase (Bioline). The PCR condition for the first step was as follows: 95 degrees C for 10 m, followed by 30 cycles of 94 degrees C for 30s, 53 degrees C for 30 s and 72 degrees C for 90 s, and a final extension at 72 degrees C for 7 m. Second PCR: Targeting a 0.5-kb fragment of ITS2 and partial LSU regions with the forward primer ITS3_KYO2 (Toju et al. 2012) fused with the 454 Adaptor A sequence and a multiplex identifier (MID) tag sequence (Hamady et al. 2008) to identify source soil samples (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG [Adaptor A] ?xxxxxxxx [MID] -GAT GAA GAA CGY AGY RAA-3' [ITS3_KYO2]), and the reverse primer LR_KYO1b fused with the 454 Adaptor B sequence (5'-CCT ATC CCC TGT GTG CCT TGG CAG TCT CAG [Adaptor B] -MGC WGC ATT CCC AAA CWA-3' [LR_KYO1b]). The second PCR was conducted with the buffer system of Taq DNA Polymerase with Standard Taq Buffer (New England BioLabs) under a temperature profile of 95 degrees C for 2 m, followed by 40 cycles of 94 degrees C for 30 s, 50 degrees C for 30 s and 72 degrees C for 60 s, and final extension step at 72 degrees C for 7 m. 0 Technical Read Adapter TCAG 1 barcode_tag Technical Read BarCode AAGCATGG 2 Technical Read Primer GATGAAGAACGYAGYRAA 3 Application Read Forward 31 454 GS Junior 1 NIL PRJDB2323 DNA extraction from individual soil sample (0.10 g dry weight per extraction) by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00010047 HESHY4K01__AAGCCGAA__ITS AMPLICON METAGENOMIC PCR pubmed 22808280 First PCR: PCR-amplified with the fungus-specific high-coverage primer ITS1-F_KYO2 (5'-TAG AGG AAG TAA AAG TCG TAA-3'; Toju et al. 2012) and the universal primer LR3 (5'-CCG TGT TTC AAG ACG GG-3'; Vilgalys and Gonzalez 1990). The first PCR was performed with the buffer system of Ampdirect Plus (Shimadzu) and BIOTAQ HS DNA Polymerase (Bioline). The PCR condition for the first step was as follows: 95 degrees C for 10 m, followed by 30 cycles of 94 degrees C for 30s, 53 degrees C for 30 s and 72 degrees C for 90 s, and a final extension at 72 degrees C for 7 m. Second PCR: Targeting a 0.5-kb fragment of ITS2 and partial LSU regions with the forward primer ITS3_KYO2 (Toju et al. 2012) fused with the 454 Adaptor A sequence and a multiplex identifier (MID) tag sequence (Hamady et al. 2008) to identify source soil samples (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG [Adaptor A] ?xxxxxxxx [MID] -GAT GAA GAA CGY AGY RAA-3' [ITS3_KYO2]), and the reverse primer LR_KYO1b fused with the 454 Adaptor B sequence (5'-CCT ATC CCC TGT GTG CCT TGG CAG TCT CAG [Adaptor B] -MGC WGC ATT CCC AAA CWA-3' [LR_KYO1b]). The second PCR was conducted with the buffer system of Taq DNA Polymerase with Standard Taq Buffer (New England BioLabs) under a temperature profile of 95 degrees C for 2 m, followed by 40 cycles of 94 degrees C for 30 s, 50 degrees C for 30 s and 72 degrees C for 60 s, and final extension step at 72 degrees C for 7 m. 0 Technical Read Adapter TCAG 1 barcode_tag Technical Read BarCode AAGCCGAA 2 Technical Read Primer GATGAAGAACGYAGYRAA 3 Application Read Forward 31 454 GS Junior 1 NIL PRJDB2323 DNA extraction from individual soil sample (0.10 g dry weight per extraction) by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00010033 HESHY4K01__AAGCCGTT__ITS AMPLICON METAGENOMIC PCR pubmed 22808280 First PCR: PCR-amplified with the fungus-specific high-coverage primer ITS1-F_KYO2 (5'-TAG AGG AAG TAA AAG TCG TAA-3'; Toju et al. 2012) and the universal primer LR3 (5'-CCG TGT TTC AAG ACG GG-3'; Vilgalys and Gonzalez 1990). The first PCR was performed with the buffer system of Ampdirect Plus (Shimadzu) and BIOTAQ HS DNA Polymerase (Bioline). The PCR condition for the first step was as follows: 95 degrees C for 10 m, followed by 30 cycles of 94 degrees C for 30s, 53 degrees C for 30 s and 72 degrees C for 90 s, and a final extension at 72 degrees C for 7 m. Second PCR: Targeting a 0.5-kb fragment of ITS2 and partial LSU regions with the forward primer ITS3_KYO2 (Toju et al. 2012) fused with the 454 Adaptor A sequence and a multiplex identifier (MID) tag sequence (Hamady et al. 2008) to identify source soil samples (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG [Adaptor A] ?xxxxxxxx [MID] -GAT GAA GAA CGY AGY RAA-3' [ITS3_KYO2]), and the reverse primer LR_KYO1b fused with the 454 Adaptor B sequence (5'-CCT ATC CCC TGT GTG CCT TGG CAG TCT CAG [Adaptor B] -MGC WGC ATT CCC AAA CWA-3' [LR_KYO1b]). The second PCR was conducted with the buffer system of Taq DNA Polymerase with Standard Taq Buffer (New England BioLabs) under a temperature profile of 95 degrees C for 2 m, followed by 40 cycles of 94 degrees C for 30 s, 50 degrees C for 30 s and 72 degrees C for 60 s, and final extension step at 72 degrees C for 7 m. 0 Technical Read Adapter TCAG 1 barcode_tag Technical Read BarCode AAGCCGTT 2 Technical Read Primer GATGAAGAACGYAGYRAA 3 Application Read Forward 31 454 GS Junior 1 NIL PRJDB2323 DNA extraction from individual soil sample (0.10 g dry weight per extraction) by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00010037 HESHY4K01__AAGCGCAA__ITS AMPLICON METAGENOMIC PCR pubmed 22808280 First PCR: PCR-amplified with the fungus-specific high-coverage primer ITS1-F_KYO2 (5'-TAG AGG AAG TAA AAG TCG TAA-3'; Toju et al. 2012) and the universal primer LR3 (5'-CCG TGT TTC AAG ACG GG-3'; Vilgalys and Gonzalez 1990). The first PCR was performed with the buffer system of Ampdirect Plus (Shimadzu) and BIOTAQ HS DNA Polymerase (Bioline). The PCR condition for the first step was as follows: 95 degrees C for 10 m, followed by 30 cycles of 94 degrees C for 30s, 53 degrees C for 30 s and 72 degrees C for 90 s, and a final extension at 72 degrees C for 7 m. Second PCR: Targeting a 0.5-kb fragment of ITS2 and partial LSU regions with the forward primer ITS3_KYO2 (Toju et al. 2012) fused with the 454 Adaptor A sequence and a multiplex identifier (MID) tag sequence (Hamady et al. 2008) to identify source soil samples (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG [Adaptor A] ?xxxxxxxx [MID] -GAT GAA GAA CGY AGY RAA-3' [ITS3_KYO2]), and the reverse primer LR_KYO1b fused with the 454 Adaptor B sequence (5'-CCT ATC CCC TGT GTG CCT TGG CAG TCT CAG [Adaptor B] -MGC WGC ATT CCC AAA CWA-3' [LR_KYO1b]). The second PCR was conducted with the buffer system of Taq DNA Polymerase with Standard Taq Buffer (New England BioLabs) under a temperature profile of 95 degrees C for 2 m, followed by 40 cycles of 94 degrees C for 30 s, 50 degrees C for 30 s and 72 degrees C for 60 s, and final extension step at 72 degrees C for 7 m. 0 Technical Read Adapter TCAG 1 barcode_tag Technical Read BarCode AAGCGCAA 2 Technical Read Primer GATGAAGAACGYAGYRAA 3 Application Read Forward 31 454 GS Junior 1 NIL PRJDB2323 DNA extraction from individual soil sample (0.10 g dry weight per extraction) by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00010045 HESHY4K01__AAGCGCTT__ITS AMPLICON METAGENOMIC PCR pubmed 22808280 First PCR: PCR-amplified with the fungus-specific high-coverage primer ITS1-F_KYO2 (5'-TAG AGG AAG TAA AAG TCG TAA-3'; Toju et al. 2012) and the universal primer LR3 (5'-CCG TGT TTC AAG ACG GG-3'; Vilgalys and Gonzalez 1990). The first PCR was performed with the buffer system of Ampdirect Plus (Shimadzu) and BIOTAQ HS DNA Polymerase (Bioline). The PCR condition for the first step was as follows: 95 degrees C for 10 m, followed by 30 cycles of 94 degrees C for 30s, 53 degrees C for 30 s and 72 degrees C for 90 s, and a final extension at 72 degrees C for 7 m. Second PCR: Targeting a 0.5-kb fragment of ITS2 and partial LSU regions with the forward primer ITS3_KYO2 (Toju et al. 2012) fused with the 454 Adaptor A sequence and a multiplex identifier (MID) tag sequence (Hamady et al. 2008) to identify source soil samples (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG [Adaptor A] ?xxxxxxxx [MID] -GAT GAA GAA CGY AGY RAA-3' [ITS3_KYO2]), and the reverse primer LR_KYO1b fused with the 454 Adaptor B sequence (5'-CCT ATC CCC TGT GTG CCT TGG CAG TCT CAG [Adaptor B] -MGC WGC ATT CCC AAA CWA-3' [LR_KYO1b]). The second PCR was conducted with the buffer system of Taq DNA Polymerase with Standard Taq Buffer (New England BioLabs) under a temperature profile of 95 degrees C for 2 m, followed by 40 cycles of 94 degrees C for 30 s, 50 degrees C for 30 s and 72 degrees C for 60 s, and final extension step at 72 degrees C for 7 m. 0 Technical Read Adapter TCAG 1 barcode_tag Technical Read BarCode AAGCGCTT 2 Technical Read Primer GATGAAGAACGYAGYRAA 3 Application Read Forward 31 454 GS Junior 1 NIL PRJDB2323 DNA extraction from individual soil sample (0.10 g dry weight per extraction) by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00010041 HESHY4K01__AAGCGGAT__ITS AMPLICON METAGENOMIC PCR pubmed 22808280 First PCR: PCR-amplified with the fungus-specific high-coverage primer ITS1-F_KYO2 (5'-TAG AGG AAG TAA AAG TCG TAA-3'; Toju et al. 2012) and the universal primer LR3 (5'-CCG TGT TTC AAG ACG GG-3'; Vilgalys and Gonzalez 1990). The first PCR was performed with the buffer system of Ampdirect Plus (Shimadzu) and BIOTAQ HS DNA Polymerase (Bioline). The PCR condition for the first step was as follows: 95 degrees C for 10 m, followed by 30 cycles of 94 degrees C for 30s, 53 degrees C for 30 s and 72 degrees C for 90 s, and a final extension at 72 degrees C for 7 m. Second PCR: Targeting a 0.5-kb fragment of ITS2 and partial LSU regions with the forward primer ITS3_KYO2 (Toju et al. 2012) fused with the 454 Adaptor A sequence and a multiplex identifier (MID) tag sequence (Hamady et al. 2008) to identify source soil samples (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG [Adaptor A] ?xxxxxxxx [MID] -GAT GAA GAA CGY AGY RAA-3' [ITS3_KYO2]), and the reverse primer LR_KYO1b fused with the 454 Adaptor B sequence (5'-CCT ATC CCC TGT GTG CCT TGG CAG TCT CAG [Adaptor B] -MGC WGC ATT CCC AAA CWA-3' [LR_KYO1b]). The second PCR was conducted with the buffer system of Taq DNA Polymerase with Standard Taq Buffer (New England BioLabs) under a temperature profile of 95 degrees C for 2 m, followed by 40 cycles of 94 degrees C for 30 s, 50 degrees C for 30 s and 72 degrees C for 60 s, and final extension step at 72 degrees C for 7 m. 0 Technical Read Adapter TCAG 1 barcode_tag Technical Read BarCode AAGCGGAT 2 Technical Read Primer GATGAAGAACGYAGYRAA 3 Application Read Forward 31 454 GS Junior 1 NIL PRJDB2323 DNA extraction from individual soil sample (0.10 g dry weight per extraction) by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00010040 HESHY4K01__AAGCGGTA__ITS AMPLICON METAGENOMIC PCR pubmed 22808280 First PCR: PCR-amplified with the fungus-specific high-coverage primer ITS1-F_KYO2 (5'-TAG AGG AAG TAA AAG TCG TAA-3'; Toju et al. 2012) and the universal primer LR3 (5'-CCG TGT TTC AAG ACG GG-3'; Vilgalys and Gonzalez 1990). The first PCR was performed with the buffer system of Ampdirect Plus (Shimadzu) and BIOTAQ HS DNA Polymerase (Bioline). The PCR condition for the first step was as follows: 95 degrees C for 10 m, followed by 30 cycles of 94 degrees C for 30s, 53 degrees C for 30 s and 72 degrees C for 90 s, and a final extension at 72 degrees C for 7 m. Second PCR: Targeting a 0.5-kb fragment of ITS2 and partial LSU regions with the forward primer ITS3_KYO2 (Toju et al. 2012) fused with the 454 Adaptor A sequence and a multiplex identifier (MID) tag sequence (Hamady et al. 2008) to identify source soil samples (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG [Adaptor A] ?xxxxxxxx [MID] -GAT GAA GAA CGY AGY RAA-3' [ITS3_KYO2]), and the reverse primer LR_KYO1b fused with the 454 Adaptor B sequence (5'-CCT ATC CCC TGT GTG CCT TGG CAG TCT CAG [Adaptor B] -MGC WGC ATT CCC AAA CWA-3' [LR_KYO1b]). The second PCR was conducted with the buffer system of Taq DNA Polymerase with Standard Taq Buffer (New England BioLabs) under a temperature profile of 95 degrees C for 2 m, followed by 40 cycles of 94 degrees C for 30 s, 50 degrees C for 30 s and 72 degrees C for 60 s, and final extension step at 72 degrees C for 7 m. 0 Technical Read Adapter TCAG 1 barcode_tag Technical Read BarCode AAGCGGTA 2 Technical Read Primer GATGAAGAACGYAGYRAA 3 Application Read Forward 31 454 GS Junior 1 NIL PRJDB2323 DNA extraction from individual soil sample (0.10 g dry weight per extraction) by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00010039 HESHY4K01__AAGCTACC__ITS AMPLICON METAGENOMIC PCR pubmed 22808280 First PCR: PCR-amplified with the fungus-specific high-coverage primer ITS1-F_KYO2 (5'-TAG AGG AAG TAA AAG TCG TAA-3'; Toju et al. 2012) and the universal primer LR3 (5'-CCG TGT TTC AAG ACG GG-3'; Vilgalys and Gonzalez 1990). The first PCR was performed with the buffer system of Ampdirect Plus (Shimadzu) and BIOTAQ HS DNA Polymerase (Bioline). The PCR condition for the first step was as follows: 95 degrees C for 10 m, followed by 30 cycles of 94 degrees C for 30s, 53 degrees C for 30 s and 72 degrees C for 90 s, and a final extension at 72 degrees C for 7 m. Second PCR: Targeting a 0.5-kb fragment of ITS2 and partial LSU regions with the forward primer ITS3_KYO2 (Toju et al. 2012) fused with the 454 Adaptor A sequence and a multiplex identifier (MID) tag sequence (Hamady et al. 2008) to identify source soil samples (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG [Adaptor A] ?xxxxxxxx [MID] -GAT GAA GAA CGY AGY RAA-3' [ITS3_KYO2]), and the reverse primer LR_KYO1b fused with the 454 Adaptor B sequence (5'-CCT ATC CCC TGT GTG CCT TGG CAG TCT CAG [Adaptor B] -MGC WGC ATT CCC AAA CWA-3' [LR_KYO1b]). The second PCR was conducted with the buffer system of Taq DNA Polymerase with Standard Taq Buffer (New England BioLabs) under a temperature profile of 95 degrees C for 2 m, followed by 40 cycles of 94 degrees C for 30 s, 50 degrees C for 30 s and 72 degrees C for 60 s, and final extension step at 72 degrees C for 7 m. 0 Technical Read Adapter TCAG 1 barcode_tag Technical Read BarCode AAGCTACC 2 Technical Read Primer GATGAAGAACGYAGYRAA 3 Application Read Forward 31 454 GS Junior 1 NIL PRJDB2323 DNA extraction from individual soil sample (0.10 g dry weight per extraction) by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00010043 HESHY4K01__AAGCTAGG__ITS AMPLICON METAGENOMIC PCR pubmed 22808280 First PCR: PCR-amplified with the fungus-specific high-coverage primer ITS1-F_KYO2 (5'-TAG AGG AAG TAA AAG TCG TAA-3'; Toju et al. 2012) and the universal primer LR3 (5'-CCG TGT TTC AAG ACG GG-3'; Vilgalys and Gonzalez 1990). The first PCR was performed with the buffer system of Ampdirect Plus (Shimadzu) and BIOTAQ HS DNA Polymerase (Bioline). The PCR condition for the first step was as follows: 95 degrees C for 10 m, followed by 30 cycles of 94 degrees C for 30s, 53 degrees C for 30 s and 72 degrees C for 90 s, and a final extension at 72 degrees C for 7 m. Second PCR: Targeting a 0.5-kb fragment of ITS2 and partial LSU regions with the forward primer ITS3_KYO2 (Toju et al. 2012) fused with the 454 Adaptor A sequence and a multiplex identifier (MID) tag sequence (Hamady et al. 2008) to identify source soil samples (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG [Adaptor A] ?xxxxxxxx [MID] -GAT GAA GAA CGY AGY RAA-3' [ITS3_KYO2]), and the reverse primer LR_KYO1b fused with the 454 Adaptor B sequence (5'-CCT ATC CCC TGT GTG CCT TGG CAG TCT CAG [Adaptor B] -MGC WGC ATT CCC AAA CWA-3' [LR_KYO1b]). The second PCR was conducted with the buffer system of Taq DNA Polymerase with Standard Taq Buffer (New England BioLabs) under a temperature profile of 95 degrees C for 2 m, followed by 40 cycles of 94 degrees C for 30 s, 50 degrees C for 30 s and 72 degrees C for 60 s, and final extension step at 72 degrees C for 7 m. 0 Technical Read Adapter TCAG 1 barcode_tag Technical Read BarCode AAGCTAGG 2 Technical Read Primer GATGAAGAACGYAGYRAA 3 Application Read Forward 31 454 GS Junior 1 NIL PRJDB2323 DNA extraction from individual soil sample (0.10 g dry weight per extraction) by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00010071 HESHY4K01__AAGCTTCG__ITS AMPLICON METAGENOMIC PCR pubmed 22808280 First PCR: PCR-amplified with the fungus-specific high-coverage primer ITS1-F_KYO2 (5'-TAG AGG AAG TAA AAG TCG TAA-3'; Toju et al. 2012) and the universal primer LR3 (5'-CCG TGT TTC AAG ACG GG-3'; Vilgalys and Gonzalez 1990). The first PCR was performed with the buffer system of Ampdirect Plus (Shimadzu) and BIOTAQ HS DNA Polymerase (Bioline). The PCR condition for the first step was as follows: 95 degrees C for 10 m, followed by 30 cycles of 94 degrees C for 30s, 53 degrees C for 30 s and 72 degrees C for 90 s, and a final extension at 72 degrees C for 7 m. Second PCR: Targeting a 0.5-kb fragment of ITS2 and partial LSU regions with the forward primer ITS3_KYO2 (Toju et al. 2012) fused with the 454 Adaptor A sequence and a multiplex identifier (MID) tag sequence (Hamady et al. 2008) to identify source soil samples (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG [Adaptor A] ?xxxxxxxx [MID] -GAT GAA GAA CGY AGY RAA-3' [ITS3_KYO2]), and the reverse primer LR_KYO1b fused with the 454 Adaptor B sequence (5'-CCT ATC CCC TGT GTG CCT TGG CAG TCT CAG [Adaptor B] -MGC WGC ATT CCC AAA CWA-3' [LR_KYO1b]). The second PCR was conducted with the buffer system of Taq DNA Polymerase with Standard Taq Buffer (New England BioLabs) under a temperature profile of 95 degrees C for 2 m, followed by 40 cycles of 94 degrees C for 30 s, 50 degrees C for 30 s and 72 degrees C for 60 s, and final extension step at 72 degrees C for 7 m. 0 Technical Read Adapter TCAG 1 barcode_tag Technical Read BarCode AAGCTTCG 2 Technical Read Primer GATGAAGAACGYAGYRAA 3 Application Read Forward 31 454 GS Junior 1 NIL PRJDB2323 DNA extraction from individual soil sample (0.10 g dry weight per extraction) by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00010062 HESHY4K01__AAGCTTGC__ITS AMPLICON METAGENOMIC PCR pubmed 22808280 First PCR: PCR-amplified with the fungus-specific high-coverage primer ITS1-F_KYO2 (5'-TAG AGG AAG TAA AAG TCG TAA-3'; Toju et al. 2012) and the universal primer LR3 (5'-CCG TGT TTC AAG ACG GG-3'; Vilgalys and Gonzalez 1990). The first PCR was performed with the buffer system of Ampdirect Plus (Shimadzu) and BIOTAQ HS DNA Polymerase (Bioline). The PCR condition for the first step was as follows: 95 degrees C for 10 m, followed by 30 cycles of 94 degrees C for 30s, 53 degrees C for 30 s and 72 degrees C for 90 s, and a final extension at 72 degrees C for 7 m. Second PCR: Targeting a 0.5-kb fragment of ITS2 and partial LSU regions with the forward primer ITS3_KYO2 (Toju et al. 2012) fused with the 454 Adaptor A sequence and a multiplex identifier (MID) tag sequence (Hamady et al. 2008) to identify source soil samples (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG [Adaptor A] ?xxxxxxxx [MID] -GAT GAA GAA CGY AGY RAA-3' [ITS3_KYO2]), and the reverse primer LR_KYO1b fused with the 454 Adaptor B sequence (5'-CCT ATC CCC TGT GTG CCT TGG CAG TCT CAG [Adaptor B] -MGC WGC ATT CCC AAA CWA-3' [LR_KYO1b]). The second PCR was conducted with the buffer system of Taq DNA Polymerase with Standard Taq Buffer (New England BioLabs) under a temperature profile of 95 degrees C for 2 m, followed by 40 cycles of 94 degrees C for 30 s, 50 degrees C for 30 s and 72 degrees C for 60 s, and final extension step at 72 degrees C for 7 m. 0 Technical Read Adapter TCAG 1 barcode_tag Technical Read BarCode AAGCTTGC 2 Technical Read Primer GATGAAGAACGYAGYRAA 3 Application Read Forward 31 454 GS Junior 1 NIL PRJDB2323 DNA extraction from individual soil sample (0.10 g dry weight per extraction) by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00010067 HESHY4K01__AAGGAACC__ITS AMPLICON METAGENOMIC PCR pubmed 22808280 First PCR: PCR-amplified with the fungus-specific high-coverage primer ITS1-F_KYO2 (5'-TAG AGG AAG TAA AAG TCG TAA-3'; Toju et al. 2012) and the universal primer LR3 (5'-CCG TGT TTC AAG ACG GG-3'; Vilgalys and Gonzalez 1990). The first PCR was performed with the buffer system of Ampdirect Plus (Shimadzu) and BIOTAQ HS DNA Polymerase (Bioline). The PCR condition for the first step was as follows: 95 degrees C for 10 m, followed by 30 cycles of 94 degrees C for 30s, 53 degrees C for 30 s and 72 degrees C for 90 s, and a final extension at 72 degrees C for 7 m. Second PCR: Targeting a 0.5-kb fragment of ITS2 and partial LSU regions with the forward primer ITS3_KYO2 (Toju et al. 2012) fused with the 454 Adaptor A sequence and a multiplex identifier (MID) tag sequence (Hamady et al. 2008) to identify source soil samples (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG [Adaptor A] ?xxxxxxxx [MID] -GAT GAA GAA CGY AGY RAA-3' [ITS3_KYO2]), and the reverse primer LR_KYO1b fused with the 454 Adaptor B sequence (5'-CCT ATC CCC TGT GTG CCT TGG CAG TCT CAG [Adaptor B] -MGC WGC ATT CCC AAA CWA-3' [LR_KYO1b]). The second PCR was conducted with the buffer system of Taq DNA Polymerase with Standard Taq Buffer (New England BioLabs) under a temperature profile of 95 degrees C for 2 m, followed by 40 cycles of 94 degrees C for 30 s, 50 degrees C for 30 s and 72 degrees C for 60 s, and final extension step at 72 degrees C for 7 m. 0 Technical Read Adapter TCAG 1 barcode_tag Technical Read BarCode AAGGAACC 2 Technical Read Primer GATGAAGAACGYAGYRAA 3 Application Read Forward 31 454 GS Junior 1 NIL PRJDB2323 DNA extraction from individual soil sample (0.10 g dry weight per extraction) by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00010044 HESHY4K01__AAGGAAGG__ITS AMPLICON METAGENOMIC PCR pubmed 22808280 First PCR: PCR-amplified with the fungus-specific high-coverage primer ITS1-F_KYO2 (5'-TAG AGG AAG TAA AAG TCG TAA-3'; Toju et al. 2012) and the universal primer LR3 (5'-CCG TGT TTC AAG ACG GG-3'; Vilgalys and Gonzalez 1990). The first PCR was performed with the buffer system of Ampdirect Plus (Shimadzu) and BIOTAQ HS DNA Polymerase (Bioline). The PCR condition for the first step was as follows: 95 degrees C for 10 m, followed by 30 cycles of 94 degrees C for 30s, 53 degrees C for 30 s and 72 degrees C for 90 s, and a final extension at 72 degrees C for 7 m. Second PCR: Targeting a 0.5-kb fragment of ITS2 and partial LSU regions with the forward primer ITS3_KYO2 (Toju et al. 2012) fused with the 454 Adaptor A sequence and a multiplex identifier (MID) tag sequence (Hamady et al. 2008) to identify source soil samples (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG [Adaptor A] ?xxxxxxxx [MID] -GAT GAA GAA CGY AGY RAA-3' [ITS3_KYO2]), and the reverse primer LR_KYO1b fused with the 454 Adaptor B sequence (5'-CCT ATC CCC TGT GTG CCT TGG CAG TCT CAG [Adaptor B] -MGC WGC ATT CCC AAA CWA-3' [LR_KYO1b]). The second PCR was conducted with the buffer system of Taq DNA Polymerase with Standard Taq Buffer (New England BioLabs) under a temperature profile of 95 degrees C for 2 m, followed by 40 cycles of 94 degrees C for 30 s, 50 degrees C for 30 s and 72 degrees C for 60 s, and final extension step at 72 degrees C for 7 m. 0 Technical Read Adapter TCAG 1 barcode_tag Technical Read BarCode AAGGAAGG 2 Technical Read Primer GATGAAGAACGYAGYRAA 3 Application Read Forward 31 454 GS Junior 1 NIL PRJDB2323 DNA extraction from individual soil sample (0.10 g dry weight per extraction) by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00010056 HESHY4K01__AAGGATCG__ITS AMPLICON METAGENOMIC PCR pubmed 22808280 First PCR: PCR-amplified with the fungus-specific high-coverage primer ITS1-F_KYO2 (5'-TAG AGG AAG TAA AAG TCG TAA-3'; Toju et al. 2012) and the universal primer LR3 (5'-CCG TGT TTC AAG ACG GG-3'; Vilgalys and Gonzalez 1990). The first PCR was performed with the buffer system of Ampdirect Plus (Shimadzu) and BIOTAQ HS DNA Polymerase (Bioline). The PCR condition for the first step was as follows: 95 degrees C for 10 m, followed by 30 cycles of 94 degrees C for 30s, 53 degrees C for 30 s and 72 degrees C for 90 s, and a final extension at 72 degrees C for 7 m. Second PCR: Targeting a 0.5-kb fragment of ITS2 and partial LSU regions with the forward primer ITS3_KYO2 (Toju et al. 2012) fused with the 454 Adaptor A sequence and a multiplex identifier (MID) tag sequence (Hamady et al. 2008) to identify source soil samples (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG [Adaptor A] ?xxxxxxxx [MID] -GAT GAA GAA CGY AGY RAA-3' [ITS3_KYO2]), and the reverse primer LR_KYO1b fused with the 454 Adaptor B sequence (5'-CCT ATC CCC TGT GTG CCT TGG CAG TCT CAG [Adaptor B] -MGC WGC ATT CCC AAA CWA-3' [LR_KYO1b]). The second PCR was conducted with the buffer system of Taq DNA Polymerase with Standard Taq Buffer (New England BioLabs) under a temperature profile of 95 degrees C for 2 m, followed by 40 cycles of 94 degrees C for 30 s, 50 degrees C for 30 s and 72 degrees C for 60 s, and final extension step at 72 degrees C for 7 m. 0 Technical Read Adapter TCAG 1 barcode_tag Technical Read BarCode AAGGATCG 2 Technical Read Primer GATGAAGAACGYAGYRAA 3 Application Read Forward 31 454 GS Junior 1 NIL PRJDB2323 DNA extraction from individual soil sample (0.10 g dry weight per extraction) by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00010057 HESHY4K01__AAGGATGC__ITS AMPLICON METAGENOMIC PCR pubmed 22808280 First PCR: PCR-amplified with the fungus-specific high-coverage primer ITS1-F_KYO2 (5'-TAG AGG AAG TAA AAG TCG TAA-3'; Toju et al. 2012) and the universal primer LR3 (5'-CCG TGT TTC AAG ACG GG-3'; Vilgalys and Gonzalez 1990). The first PCR was performed with the buffer system of Ampdirect Plus (Shimadzu) and BIOTAQ HS DNA Polymerase (Bioline). The PCR condition for the first step was as follows: 95 degrees C for 10 m, followed by 30 cycles of 94 degrees C for 30s, 53 degrees C for 30 s and 72 degrees C for 90 s, and a final extension at 72 degrees C for 7 m. Second PCR: Targeting a 0.5-kb fragment of ITS2 and partial LSU regions with the forward primer ITS3_KYO2 (Toju et al. 2012) fused with the 454 Adaptor A sequence and a multiplex identifier (MID) tag sequence (Hamady et al. 2008) to identify source soil samples (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG [Adaptor A] ?xxxxxxxx [MID] -GAT GAA GAA CGY AGY RAA-3' [ITS3_KYO2]), and the reverse primer LR_KYO1b fused with the 454 Adaptor B sequence (5'-CCT ATC CCC TGT GTG CCT TGG CAG TCT CAG [Adaptor B] -MGC WGC ATT CCC AAA CWA-3' [LR_KYO1b]). The second PCR was conducted with the buffer system of Taq DNA Polymerase with Standard Taq Buffer (New England BioLabs) under a temperature profile of 95 degrees C for 2 m, followed by 40 cycles of 94 degrees C for 30 s, 50 degrees C for 30 s and 72 degrees C for 60 s, and final extension step at 72 degrees C for 7 m. 0 Technical Read Adapter TCAG 1 barcode_tag Technical Read BarCode AAGGATGC 2 Technical Read Primer GATGAAGAACGYAGYRAA 3 Application Read Forward 31 454 GS Junior 1 NIL PRJDB2323 DNA extraction from individual soil sample (0.10 g dry weight per extraction) by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00010055 HESHY4K01__AAGGCCAA__ITS AMPLICON METAGENOMIC PCR pubmed 22808280 First PCR: PCR-amplified with the fungus-specific high-coverage primer ITS1-F_KYO2 (5'-TAG AGG AAG TAA AAG TCG TAA-3'; Toju et al. 2012) and the universal primer LR3 (5'-CCG TGT TTC AAG ACG GG-3'; Vilgalys and Gonzalez 1990). The first PCR was performed with the buffer system of Ampdirect Plus (Shimadzu) and BIOTAQ HS DNA Polymerase (Bioline). The PCR condition for the first step was as follows: 95 degrees C for 10 m, followed by 30 cycles of 94 degrees C for 30s, 53 degrees C for 30 s and 72 degrees C for 90 s, and a final extension at 72 degrees C for 7 m. Second PCR: Targeting a 0.5-kb fragment of ITS2 and partial LSU regions with the forward primer ITS3_KYO2 (Toju et al. 2012) fused with the 454 Adaptor A sequence and a multiplex identifier (MID) tag sequence (Hamady et al. 2008) to identify source soil samples (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG [Adaptor A] ?xxxxxxxx [MID] -GAT GAA GAA CGY AGY RAA-3' [ITS3_KYO2]), and the reverse primer LR_KYO1b fused with the 454 Adaptor B sequence (5'-CCT ATC CCC TGT GTG CCT TGG CAG TCT CAG [Adaptor B] -MGC WGC ATT CCC AAA CWA-3' [LR_KYO1b]). The second PCR was conducted with the buffer system of Taq DNA Polymerase with Standard Taq Buffer (New England BioLabs) under a temperature profile of 95 degrees C for 2 m, followed by 40 cycles of 94 degrees C for 30 s, 50 degrees C for 30 s and 72 degrees C for 60 s, and final extension step at 72 degrees C for 7 m. 0 Technical Read Adapter TCAG 1 barcode_tag Technical Read BarCode AAGGCCAA 2 Technical Read Primer GATGAAGAACGYAGYRAA 3 Application Read Forward 31 454 GS Junior 1 NIL PRJDB2323 DNA extraction from individual soil sample (0.10 g dry weight per extraction) by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00010036 HESHY4K01__AAGGCCTT__ITS AMPLICON METAGENOMIC PCR pubmed 22808280 First PCR: PCR-amplified with the fungus-specific high-coverage primer ITS1-F_KYO2 (5'-TAG AGG AAG TAA AAG TCG TAA-3'; Toju et al. 2012) and the universal primer LR3 (5'-CCG TGT TTC AAG ACG GG-3'; Vilgalys and Gonzalez 1990). The first PCR was performed with the buffer system of Ampdirect Plus (Shimadzu) and BIOTAQ HS DNA Polymerase (Bioline). The PCR condition for the first step was as follows: 95 degrees C for 10 m, followed by 30 cycles of 94 degrees C for 30s, 53 degrees C for 30 s and 72 degrees C for 90 s, and a final extension at 72 degrees C for 7 m. Second PCR: Targeting a 0.5-kb fragment of ITS2 and partial LSU regions with the forward primer ITS3_KYO2 (Toju et al. 2012) fused with the 454 Adaptor A sequence and a multiplex identifier (MID) tag sequence (Hamady et al. 2008) to identify source soil samples (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG [Adaptor A] ?xxxxxxxx [MID] -GAT GAA GAA CGY AGY RAA-3' [ITS3_KYO2]), and the reverse primer LR_KYO1b fused with the 454 Adaptor B sequence (5'-CCT ATC CCC TGT GTG CCT TGG CAG TCT CAG [Adaptor B] -MGC WGC ATT CCC AAA CWA-3' [LR_KYO1b]). The second PCR was conducted with the buffer system of Taq DNA Polymerase with Standard Taq Buffer (New England BioLabs) under a temperature profile of 95 degrees C for 2 m, followed by 40 cycles of 94 degrees C for 30 s, 50 degrees C for 30 s and 72 degrees C for 60 s, and final extension step at 72 degrees C for 7 m. 0 Technical Read Adapter TCAG 1 barcode_tag Technical Read BarCode AAGGCCTT 2 Technical Read Primer GATGAAGAACGYAGYRAA 3 Application Read Forward 31 454 GS Junior 1 NIL PRJDB2323 DNA extraction from individual soil sample (0.10 g dry weight per extraction) by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00010072 HESHY4K01__AAGGCGAT__ITS AMPLICON METAGENOMIC PCR pubmed 22808280 First PCR: PCR-amplified with the fungus-specific high-coverage primer ITS1-F_KYO2 (5'-TAG AGG AAG TAA AAG TCG TAA-3'; Toju et al. 2012) and the universal primer LR3 (5'-CCG TGT TTC AAG ACG GG-3'; Vilgalys and Gonzalez 1990). The first PCR was performed with the buffer system of Ampdirect Plus (Shimadzu) and BIOTAQ HS DNA Polymerase (Bioline). The PCR condition for the first step was as follows: 95 degrees C for 10 m, followed by 30 cycles of 94 degrees C for 30s, 53 degrees C for 30 s and 72 degrees C for 90 s, and a final extension at 72 degrees C for 7 m. Second PCR: Targeting a 0.5-kb fragment of ITS2 and partial LSU regions with the forward primer ITS3_KYO2 (Toju et al. 2012) fused with the 454 Adaptor A sequence and a multiplex identifier (MID) tag sequence (Hamady et al. 2008) to identify source soil samples (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG [Adaptor A] ?xxxxxxxx [MID] -GAT GAA GAA CGY AGY RAA-3' [ITS3_KYO2]), and the reverse primer LR_KYO1b fused with the 454 Adaptor B sequence (5'-CCT ATC CCC TGT GTG CCT TGG CAG TCT CAG [Adaptor B] -MGC WGC ATT CCC AAA CWA-3' [LR_KYO1b]). The second PCR was conducted with the buffer system of Taq DNA Polymerase with Standard Taq Buffer (New England BioLabs) under a temperature profile of 95 degrees C for 2 m, followed by 40 cycles of 94 degrees C for 30 s, 50 degrees C for 30 s and 72 degrees C for 60 s, and final extension step at 72 degrees C for 7 m. 0 Technical Read Adapter TCAG 1 barcode_tag Technical Read BarCode AAGGCGAT 2 Technical Read Primer GATGAAGAACGYAGYRAA 3 Application Read Forward 31 454 GS Junior 1 NIL PRJDB2323 DNA extraction from individual soil sample (0.10 g dry weight per extraction) by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00010054 HESHY4K01__AAGGCGTA__ITS AMPLICON METAGENOMIC PCR pubmed 22808280 First PCR: PCR-amplified with the fungus-specific high-coverage primer ITS1-F_KYO2 (5'-TAG AGG AAG TAA AAG TCG TAA-3'; Toju et al. 2012) and the universal primer LR3 (5'-CCG TGT TTC AAG ACG GG-3'; Vilgalys and Gonzalez 1990). The first PCR was performed with the buffer system of Ampdirect Plus (Shimadzu) and BIOTAQ HS DNA Polymerase (Bioline). The PCR condition for the first step was as follows: 95 degrees C for 10 m, followed by 30 cycles of 94 degrees C for 30s, 53 degrees C for 30 s and 72 degrees C for 90 s, and a final extension at 72 degrees C for 7 m. Second PCR: Targeting a 0.5-kb fragment of ITS2 and partial LSU regions with the forward primer ITS3_KYO2 (Toju et al. 2012) fused with the 454 Adaptor A sequence and a multiplex identifier (MID) tag sequence (Hamady et al. 2008) to identify source soil samples (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG [Adaptor A] ?xxxxxxxx [MID] -GAT GAA GAA CGY AGY RAA-3' [ITS3_KYO2]), and the reverse primer LR_KYO1b fused with the 454 Adaptor B sequence (5'-CCT ATC CCC TGT GTG CCT TGG CAG TCT CAG [Adaptor B] -MGC WGC ATT CCC AAA CWA-3' [LR_KYO1b]). The second PCR was conducted with the buffer system of Taq DNA Polymerase with Standard Taq Buffer (New England BioLabs) under a temperature profile of 95 degrees C for 2 m, followed by 40 cycles of 94 degrees C for 30 s, 50 degrees C for 30 s and 72 degrees C for 60 s, and final extension step at 72 degrees C for 7 m. 0 Technical Read Adapter TCAG 1 barcode_tag Technical Read BarCode AAGGCGTA 2 Technical Read Primer GATGAAGAACGYAGYRAA 3 Application Read Forward 31 454 GS Junior 1 NIL