PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002948 HEUG2XR01__AACCAACC__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode AACCAACC 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002874 HEUG2XR01__AACCAAGG__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode AACCAAGG 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002852 HEUG2XR01__AACCATCG__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode AACCATCG 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002995 HEUG2XR01__AACCATGC__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode AACCATGC 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002991 HEUG2XR01__AACCGCAT__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode AACCGCAT 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002942 HEUG2XR01__AACCGCTA__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode AACCGCTA 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002853 HEUG2XR01__AACCGGTT__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode AACCGGTT 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002981 HEUG2XR01__AACCTACG__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode AACCTACG 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002862 HEUG2XR01__AACCTAGC__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode AACCTAGC 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002904 HEUG2XR01__AACCTTCC__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode AACCTTCC 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002971 HEUG2XR01__AACCTTGG__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode AACCTTGG 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002973 HEUG2XR01__AACGAACG__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode AACGAACG 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002996 HEUG2XR01__AACGAAGC__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode AACGAAGC 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002923 HEUG2XR01__AACGATCC__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode AACGATCC 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002987 HEUG2XR01__AACGATGG__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode AACGATGG 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002989 HEUG2XR01__AACGCCAT__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode AACGCCAT 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002937 HEUG2XR01__AACGCCTA__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode AACGCCTA 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002843 HEUG2XR01__AACGCGTT__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode AACGCGTT 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002902 HEUG2XR01__AACGGCTT__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode AACGGCTT 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00003000 HEUG2XR01__AACGTACC__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode AACGTACC 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002918 HEUG2XR01__AACGTAGG__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode AACGTAGG 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002967 HEUG2XR01__AACGTTCG__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode AACGTTCG 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002940 HEUG2XR01__AACGTTGC__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode AACGTTGC 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002868 HEUG2XR01__AAGCAACG__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode AAGCAACG 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002905 HEUG2XR01__AAGCAAGC__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode AAGCAAGC 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002892 HEUG2XR01__AAGCATCC__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode AAGCATCC 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002889 HEUG2XR01__AAGCATGG__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode AAGCATGG 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002849 HEUG2XR01__AAGCGCTT__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode AAGCGCTT 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002836 HEUG2XR01__AAGCGGAT__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode AAGCGGAT 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002978 HEUG2XR01__AAGCGGTA__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode AAGCGGTA 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002895 HEUG2XR01__AAGCTACC__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode AAGCTACC 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002916 HEUG2XR01__AAGCTAGG__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode AAGCTAGG 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002867 HEUG2XR01__AAGCTTCG__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode AAGCTTCG 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002938 HEUG2XR01__AAGCTTGC__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode AAGCTTGC 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002969 HEUG2XR01__AAGGAACC__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode AAGGAACC 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002893 HEUG2XR01__AAGGAAGG__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode AAGGAAGG 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002965 HEUG2XR01__AAGGATCG__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode AAGGATCG 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002932 HEUG2XR01__AAGGATGC__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode AAGGATGC 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002975 HEUG2XR01__AAGGCCTT__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode AAGGCCTT 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002917 HEUG2XR01__AAGGCGAT__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode AAGGCGAT 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002890 HEUG2XR01__AAGGCGTA__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode AAGGCGTA 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002951 HEUG2XR01__AAGGTACG__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode AAGGTACG 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002872 HEUG2XR01__AAGGTTCC__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode AAGGTTCC 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002861 HEUG2XR01__AAGGTTGG__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode AAGGTTGG 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002943 HEUG2XR01__AATACGCC__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode AATACGCC 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002873 HEUG2XR01__AATAGCGG__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode AATAGCGG 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002983 HEUG2XR01__AATAGGCG__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode AATAGGCG 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002887 HEUG2XR01__AATTCGCG__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode AATTCGCG 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002856 HEUG2XR01__AATTCGGC__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode AATTCGGC 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002992 HEUG2XR01__AATTGCCG__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode AATTGCCG 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002919 HEUG2XR01__AATTGCGC__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode AATTGCGC 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002838 HEUG2XR01__AATTGGCC__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode AATTGGCC 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002945 HEUG2XR01__ACACACAC__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACACACAC 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002847 HEUG2XR01__ACACACTG__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACACACTG 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002913 HEUG2XR01__ACACAGAG__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACACAGAG 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002914 HEUG2XR01__ACACAGTC__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACACAGTC 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002924 HEUG2XR01__ACACCACA__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACACCACA 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002906 HEUG2XR01__ACACCAGT__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACACCAGT 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002865 HEUG2XR01__ACACCTCT__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACACCTCT 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002999 HEUG2XR01__ACACCTGA__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACACCTGA 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002869 HEUG2XR01__ACACGACT__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACACGACT 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002929 HEUG2XR01__ACACGAGA__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACACGAGA 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002977 HEUG2XR01__ACACGTCA__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACACGTCA 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002939 HEUG2XR01__ACACGTGT__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACACGTGT 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002994 HEUG2XR01__ACACTCAG__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACACTCAG 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002956 HEUG2XR01__ACACTCTC__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACACTCTC 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002955 HEUG2XR01__ACACTGAC__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACACTGAC 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002968 HEUG2XR01__ACACTGTG__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACACTGTG 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002835 HEUG2XR01__ACAGACAG__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACAGACAG 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002909 HEUG2XR01__ACAGACTC__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACAGACTC 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002980 HEUG2XR01__ACAGAGAC__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACAGAGAC 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002888 HEUG2XR01__ACAGAGTG__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACAGAGTG 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002900 HEUG2XR01__ACAGCACT__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACAGCACT 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002876 HEUG2XR01__ACAGCAGA__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACAGCAGA 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002927 HEUG2XR01__ACAGCTCA__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACAGCTCA 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002880 HEUG2XR01__ACAGCTGT__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACAGCTGT 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002993 HEUG2XR01__ACAGGACA__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACAGGACA 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002870 HEUG2XR01__ACAGGAGT__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACAGGAGT 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002910 HEUG2XR01__ACAGGTCT__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACAGGTCT 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002846 HEUG2XR01__ACAGGTGA__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACAGGTGA 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002879 HEUG2XR01__ACAGTCAC__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACAGTCAC 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002898 HEUG2XR01__ACAGTCTG__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACAGTCTG 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002859 HEUG2XR01__ACAGTGAG__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACAGTGAG 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002855 HEUG2XR01__ACAGTGTC__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACAGTGTC 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002841 HEUG2XR01__ACCAACCA__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACCAACCA 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002866 HEUG2XR01__ACCAACGT__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACCAACGT 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002848 HEUG2XR01__ACCAAGCT__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACCAAGCT 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002840 HEUG2XR01__ACCAAGGA__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACCAAGGA 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002864 HEUG2XR01__ACCACAAC__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACCACAAC 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002921 HEUG2XR01__ACCACATG__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACCACATG 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002984 HEUG2XR01__ACCACTAG__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACCACTAG 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00003001 HEUG2XR01__ACCACTTC__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACCACTTC 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002886 HEUG2XR01__ACCAGAAG__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACCAGAAG 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002964 HEUG2XR01__ACCAGATC__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACCAGATC 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002854 HEUG2XR01__ACCAGTAC__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACCAGTAC 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002911 HEUG2XR01__ACCAGTTG__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACCAGTTG 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002842 HEUG2XR01__ACCATCCT__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACCATCCT 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002988 HEUG2XR01__ACCATCGA__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACCATCGA 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002986 HEUG2XR01__ACCATGCA__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACCATGCA 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002920 HEUG2XR01__ACCATGGT__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACCATGGT 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002963 HEUG2XR01__ACCTACCT__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACCTACCT 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002944 HEUG2XR01__ACCTACGA__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACCTACGA 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002952 HEUG2XR01__ACCTAGCA__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACCTAGCA 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002934 HEUG2XR01__ACCTAGGT__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACCTAGGT 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002933 HEUG2XR01__ACCTCAAG__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACCTCAAG 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002922 HEUG2XR01__ACCTCATC__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACCTCATC 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002998 HEUG2XR01__ACCTCTAC__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACCTCTAC 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002860 HEUG2XR01__ACCTCTTG__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACCTCTTG 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002863 HEUG2XR01__ACCTGAAC__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACCTGAAC 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002979 HEUG2XR01__ACCTGATG__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACCTGATG 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002882 HEUG2XR01__ACCTGTTC__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACCTGTTC 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002907 HEUG2XR01__ACCTTCCA__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACCTTCCA 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002903 HEUG2XR01__ACCTTCGT__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACCTTCGT 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002925 HEUG2XR01__ACCTTGCT__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACCTTGCT 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002875 HEUG2XR01__ACCTTGGA__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACCTTGGA 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002894 HEUG2XR01__ACGAACCT__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACGAACCT 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002883 HEUG2XR01__ACGAACGA__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACGAACGA 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002897 HEUG2XR01__ACGAAGCA__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACGAAGCA 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002953 HEUG2XR01__ACGAAGGT__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACGAAGGT 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002954 HEUG2XR01__ACGACAAG__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACGACAAG 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002857 HEUG2XR01__ACGACATC__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACGACATC 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002930 HEUG2XR01__ACGACTAC__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACGACTAC 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002884 HEUG2XR01__ACGACTTG__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACGACTTG 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00003002 HEUG2XR01__ACGAGAAC__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACGAGAAC 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002908 HEUG2XR01__ACGAGATG__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACGAGATG 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002941 HEUG2XR01__ACGAGTAG__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACGAGTAG 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002926 HEUG2XR01__ACGAGTTC__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACGAGTTC 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002970 HEUG2XR01__ACGATCCA__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACGATCCA 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002972 HEUG2XR01__ACGATCGT__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACGATCGT 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002858 HEUG2XR01__ACGATGCT__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACGATGCT 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002936 HEUG2XR01__ACGATGGA__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACGATGGA 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002997 HEUG2XR01__ACGTACCA__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACGTACCA 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002832 HEUG2XR01__ACGTACGT__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACGTACGT 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002839 HEUG2XR01__ACGTAGCT__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACGTAGCT 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002974 HEUG2XR01__ACGTAGGA__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACGTAGGA 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00003004 HEUG2XR01__ACGTCAAC__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACGTCAAC 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002950 HEUG2XR01__ACGTCATG__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACGTCATG 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002990 HEUG2XR01__ACGTCTAG__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACGTCTAG 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002891 HEUG2XR01__ACGTGAAG__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACGTGAAG 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002982 HEUG2XR01__ACGTGATC__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACGTGATC 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002946 HEUG2XR01__ACGTGTAC__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACGTGTAC 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002935 HEUG2XR01__ACGTGTTG__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACGTGTTG 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002877 HEUG2XR01__ACGTTCCT__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACGTTCCT 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002896 HEUG2XR01__ACGTTCGA__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACGTTCGA 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002850 HEUG2XR01__ACGTTGCA__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACGTTGCA 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002931 HEUG2XR01__ACGTTGGT__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACGTTGGT 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002962 HEUG2XR01__ACTCACAG__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACTCACAG 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002915 HEUG2XR01__ACTCACTC__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACTCACTC 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002901 HEUG2XR01__ACTCAGAC__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACTCAGAC 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002976 HEUG2XR01__ACTCAGTG__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACTCAGTG 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002844 HEUG2XR01__ACTCCACT__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACTCCACT 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002960 HEUG2XR01__ACTCCAGA__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACTCCAGA 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002961 HEUG2XR01__ACTCCTCA__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACTCCTCA 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00003003 HEUG2XR01__ACTCCTGT__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACTCCTGT 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002912 HEUG2XR01__ACTCGACA__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACTCGACA 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002985 HEUG2XR01__ACTCGAGT__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACTCGAGT 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002881 HEUG2XR01__ACTCGTCT__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACTCGTCT 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002878 HEUG2XR01__ACTCGTGA__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACTCGTGA 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002834 HEUG2XR01__ACTCTCAC__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACTCTCAC 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002871 HEUG2XR01__ACTCTCTG__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACTCTCTG 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002958 HEUG2XR01__ACTCTGAG__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACTCTGAG 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002947 HEUG2XR01__ACTCTGTC__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACTCTGTC 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002837 HEUG2XR01__ACTGACAC__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACTGACAC 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002966 HEUG2XR01__ACTGACTG__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACTGACTG 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002949 HEUG2XR01__ACTGAGAG__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACTGAGAG 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002899 HEUG2XR01__ACTGAGTC__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACTGAGTC 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002851 HEUG2XR01__ACTGCACA__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACTGCACA 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002845 HEUG2XR01__ACTGCAGT__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACTGCAGT 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002957 HEUG2XR01__ACTGCTCT__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACTGCTCT 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002959 HEUG2XR01__ACTGCTGA__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACTGCTGA 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002885 HEUG2XR01__ACTGGACT__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACTGGACT 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002928 HEUG2XR01__ACTGGAGA__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACTGGAGA 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL PRJDB1856 DNA extraction from individual sample by a cetyl trimethyl ammonium bromide (CTAB) method. SAMD00002833 HEUG2XR01__ACTGGTCA__CYAALGF AMPLICON METAGENOMIC PCR For each sample, 16S rRNA gene were amplified using the primers CYA_ALG_F fused with the 454 pyrosequencing Adaptor A (5'-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG-3') and the 8-mer molecular ID (Hamady et al., 2008) and CYA_ALG_R with a buffer system of Ampdirect Plus (Shimadzu Corp., Kyoto, Japan) and BIOTAQ HS DNA Polymerase (Bioline, London, U.K.). Polymerase chain reaction (PCR) was conducted using a temperature profile of 95 deg C for 10 min, followed by 30 cycles at 94 deg C for 20 sec, 48 deg C for 30 sec, 72 deg C for 30 sec, and a final extension at 72 deg C for 7 min. DNA concentrations were measured using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and were diluted into 4ng/uL by Milli-Q water in each PCR sample. The amplicons were pooled and purified using ExoSAP-IT (GE Healthcare, Little Chalfont, Buckinghamshire, U.K.) and a QIAquick PCR Purification Kit (Qiagen). 0 Technical Read Adapter TCAG 1 Technical Read BarCode ACTGGTCA 2 Technical Read Primer AAACTCAAAGRAATTGACGG 3 Application Read Forward 33 454 GS Junior 1 NIL