RNA-Seq of medaka digestive tract in freshwater, replicate 1

SAMD00013904 RNA-Seq of medaka digestive tract in freshwater, replicate 1 10090 Mus musculus Medaka were bred and growth in a freshwater (FW) recirculating aquarium system in the Atmosphere and Ocean Research Institute, the University of Tokyo. The aquarium system was controlled at 26 ?C with a photoperiod of 14h/10h light/dark cycle. Fish was fed daily with freshly hatched brine shrimp. Individuals (1.0 - 1.2 g body weight) were randomly divided into nine experimental tanks. In sampling, the experimental fish was anaesthetized in 0.1% tricaine methanesulfonate (MS-222) and further sacrificed by spinal transection. Intestine was immediately dissected, snap-frozen in liquid nitrogen, and stored at -80 ?C until use. Total RNA was extracted from the intestine samples using Isogen (Nippon Gene, Toyama) according to the manufacturer protocols. Poly(A) RNA was isolated from the total RNA according to the manufacturer protocols. Strain C57BL/6J SAMD00013907 RNA-Seq of medaka digestive tract in freshwater, replicate 2 10090 Mus musculus Medaka were bred and growth in a freshwater (FW) recirculating aquarium system in the Atmosphere and Ocean Research Institute, the University of Tokyo. The aquarium system was controlled at 26 ?C with a photoperiod of 14h/10h light/dark cycle. Fish was fed daily with freshly hatched brine shrimp. Individuals (1.0 - 1.2 g body weight) were randomly divided into nine experimental tanks. In sampling, the experimental fish was anaesthetized in 0.1% tricaine methanesulfonate (MS-222) and further sacrificed by spinal transection. Intestine was immediately dissected, snap-frozen in liquid nitrogen, and stored at -80 ?C until use. Total RNA was extracted from the intestine samples using Isogen (Nippon Gene, Toyama) according to the manufacturer protocols. Poly(A) RNA was isolated from the total RNA according to the manufacturer protocols. Strain C57BL/6J SAMD00013890 RNA-Seq of medaka digestive tract in freshwater, replicate 3 10090 Mus musculus Medaka were bred and growth in a freshwater (FW) recirculating aquarium system in the Atmosphere and Ocean Research Institute, the University of Tokyo. The aquarium system was controlled at 26 ?C with a photoperiod of 14h/10h light/dark cycle. Fish was fed daily with freshly hatched brine shrimp. Individuals (1.0 - 1.2 g body weight) were randomly divided into nine experimental tanks. In sampling, the experimental fish was anaesthetized in 0.1% tricaine methanesulfonate (MS-222) and further sacrificed by spinal transection. Intestine was immediately dissected, snap-frozen in liquid nitrogen, and stored at -80 ?C until use. Total RNA was extracted from the intestine samples using Isogen (Nippon Gene, Toyama) according to the manufacturer protocols. Poly(A) RNA was isolated from the total RNA according to the manufacturer protocols. Strain C57BL/6J SAMD00013905 RNA-Seq of medaka digestive tract in freshwater, replicate 4 10090 Mus musculus Medaka were bred and growth in a freshwater (FW) recirculating aquarium system in the Atmosphere and Ocean Research Institute, the University of Tokyo. The aquarium system was controlled at 26 ?C with a photoperiod of 14h/10h light/dark cycle. Fish was fed daily with freshly hatched brine shrimp. Individuals (1.0 - 1.2 g body weight) were randomly divided into nine experimental tanks. In sampling, the experimental fish was anaesthetized in 0.1% tricaine methanesulfonate (MS-222) and further sacrificed by spinal transection. Intestine was immediately dissected, snap-frozen in liquid nitrogen, and stored at -80 ?C until use. Total RNA was extracted from the intestine samples using Isogen (Nippon Gene, Toyama) according to the manufacturer protocols. Poly(A) RNA was isolated from the total RNA according to the manufacturer protocols. Strain C57BL/6J SAMD00013902 RNA-Seq of medaka digestive tract in freshwater, replicate 5 10090 Mus musculus Medaka were bred and growth in a freshwater (FW) recirculating aquarium system in the Atmosphere and Ocean Research Institute, the University of Tokyo. The aquarium system was controlled at 26 ?C with a photoperiod of 14h/10h light/dark cycle. Fish was fed daily with freshly hatched brine shrimp. Individuals (1.0 - 1.2 g body weight) were randomly divided into nine experimental tanks. In sampling, the experimental fish was anaesthetized in 0.1% tricaine methanesulfonate (MS-222) and further sacrificed by spinal transection. Intestine was immediately dissected, snap-frozen in liquid nitrogen, and stored at -80 ?C until use. Total RNA was extracted from the intestine samples using Isogen (Nippon Gene, Toyama) according to the manufacturer protocols. Poly(A) RNA was isolated from the total RNA according to the manufacturer protocols. Strain C57BL/6J SAMD00013903 RNA-Seq of medaka digestive tract 1 hour after transfer from freshwater to seawater, replicate 1 10090 Mus musculus Medaka were bred and growth in a freshwater recirculating aquarium system in the Atmosphere and Ocean Research Institute, the University of Tokyo. The aquarium system was controlled at 26 ?C with a photoperiod of 14h/10h light/dark cycle. Fish was fed daily with freshly hatched brine shrimp. Individuals (1.0 - 1.2 g body weight) were randomly divided into nine experimental tanks. Medaka were transferred to 50% seawater. In sampling, the experimental fish was anaesthetized in 0.1% tricaine methanesulfonate (MS-222) and further sacrificed by spinal transection. Intestine was immediately dissected, snap-frozen in liquid nitrogen, and stored at -80 ?C until use. Total RNA was extracted from the intestine samples using Isogen (Nippon Gene, Toyama) according to the manufacturer protocols. Poly(A) RNA was isolated from the total RNA according to the manufacturer protocols. Strain C57BL/6J SAMD00013891 RNA-Seq of medaka digestive tract 1 hour after transfer from freshwater to seawater, replicate 2 10090 Mus musculus Medaka were bred and growth in a freshwater recirculating aquarium system in the Atmosphere and Ocean Research Institute, the University of Tokyo. The aquarium system was controlled at 26 ?C with a photoperiod of 14h/10h light/dark cycle. Fish was fed daily with freshly hatched brine shrimp. Individuals (1.0 - 1.2 g body weight) were randomly divided into nine experimental tanks. Medaka were transferred to 50% seawater. In sampling, the experimental fish was anaesthetized in 0.1% tricaine methanesulfonate (MS-222) and further sacrificed by spinal transection. Intestine was immediately dissected, snap-frozen in liquid nitrogen, and stored at -80 ?C until use. Total RNA was extracted from the intestine samples using Isogen (Nippon Gene, Toyama) according to the manufacturer protocols. Poly(A) RNA was isolated from the total RNA according to the manufacturer protocols. Strain C57BL/6J SAMD00013892 RNA-Seq of medaka digestive tract 1 hour after transfer from freshwater to seawater, replicate 3 10090 Mus musculus Medaka were bred and growth in a freshwater recirculating aquarium system in the Atmosphere and Ocean Research Institute, the University of Tokyo. The aquarium system was controlled at 26 ?C with a photoperiod of 14h/10h light/dark cycle. Fish was fed daily with freshly hatched brine shrimp. Individuals (1.0 - 1.2 g body weight) were randomly divided into nine experimental tanks. Medaka were transferred to 50% seawater. In sampling, the experimental fish was anaesthetized in 0.1% tricaine methanesulfonate (MS-222) and further sacrificed by spinal transection. Intestine was immediately dissected, snap-frozen in liquid nitrogen, and stored at -80 ?C until use. Total RNA was extracted from the intestine samples using Isogen (Nippon Gene, Toyama) according to the manufacturer protocols. Poly(A) RNA was isolated from the total RNA according to the manufacturer protocols. Strain C57BL/6J SAMD00013893 RNA-Seq of medaka digestive tract 1 hour after transfer from freshwater to seawater, replicate 4 10090 Mus musculus Medaka were bred and growth in a freshwater recirculating aquarium system in the Atmosphere and Ocean Research Institute, the University of Tokyo. The aquarium system was controlled at 26 ?C with a photoperiod of 14h/10h light/dark cycle. Fish was fed daily with freshly hatched brine shrimp. Individuals (1.0 - 1.2 g body weight) were randomly divided into nine experimental tanks. Medaka were transferred to 50% seawater. In sampling, the experimental fish was anaesthetized in 0.1% tricaine methanesulfonate (MS-222) and further sacrificed by spinal transection. Intestine was immediately dissected, snap-frozen in liquid nitrogen, and stored at -80 ?C until use. Total RNA was extracted from the intestine samples using Isogen (Nippon Gene, Toyama) according to the manufacturer protocols. Poly(A) RNA was isolated from the total RNA according to the manufacturer protocols. Strain C57BL/6J SAMD00013900 RNA-Seq of medaka digestive tract 1 hour after transfer from freshwater to seawater, replicate 5 10090 Mus musculus Medaka were bred and growth in a freshwater recirculating aquarium system in the Atmosphere and Ocean Research Institute, the University of Tokyo. The aquarium system was controlled at 26 ?C with a photoperiod of 14h/10h light/dark cycle. Fish was fed daily with freshly hatched brine shrimp. Individuals (1.0 - 1.2 g body weight) were randomly divided into nine experimental tanks. Medaka were transferred to 50% seawater. In sampling, the experimental fish was anaesthetized in 0.1% tricaine methanesulfonate (MS-222) and further sacrificed by spinal transection. Intestine was immediately dissected, snap-frozen in liquid nitrogen, and stored at -80 ?C until use. Total RNA was extracted from the intestine samples using Isogen (Nippon Gene, Toyama) according to the manufacturer protocols. Poly(A) RNA was isolated from the total RNA according to the manufacturer protocols. Strain C57BL/6J SAMD00013888 RNA-Seq of medaka digestive tract 3 hour after transfer from freshwater to seawater, replicate 1 10090 Mus musculus Medaka were bred and growth in a freshwater recirculating aquarium system in the Atmosphere and Ocean Research Institute, the University of Tokyo. The aquarium system was controlled at 26 ?C with a photoperiod of 14h/10h light/dark cycle. Fish was fed daily with freshly hatched brine shrimp. Individuals (1.0 - 1.2 g body weight) were randomly divided into nine experimental tanks. Medaka were transferred to 50% seawater. In sampling, the experimental fish was anaesthetized in 0.1% tricaine methanesulfonate (MS-222) and further sacrificed by spinal transection. Intestine was immediately dissected, snap-frozen in liquid nitrogen, and stored at -80 ?C until use. Total RNA was extracted from the intestine samples using Isogen (Nippon Gene, Toyama) according to the manufacturer protocols. Poly(A) RNA was isolated from the total RNA according to the manufacturer protocols. Strain C57BL/6J SAMD00013896 RNA-Seq of medaka digestive tract 3 hour after transfer from freshwater to seawater, replicate 2 10090 Mus musculus Medaka were bred and growth in a freshwater recirculating aquarium system in the Atmosphere and Ocean Research Institute, the University of Tokyo. The aquarium system was controlled at 26 ?C with a photoperiod of 14h/10h light/dark cycle. Fish was fed daily with freshly hatched brine shrimp. Individuals (1.0 - 1.2 g body weight) were randomly divided into nine experimental tanks. Medaka were transferred to 50% seawater. In sampling, the experimental fish was anaesthetized in 0.1% tricaine methanesulfonate (MS-222) and further sacrificed by spinal transection. Intestine was immediately dissected, snap-frozen in liquid nitrogen, and stored at -80 ?C until use. Total RNA was extracted from the intestine samples using Isogen (Nippon Gene, Toyama) according to the manufacturer protocols. Poly(A) RNA was isolated from the total RNA according to the manufacturer protocols. Strain C57BL/6J SAMD00013895 RNA-Seq of medaka digestive tract 3 hour after transfer from freshwater to seawater, replicate 3 10090 Mus musculus Medaka were bred and growth in a freshwater recirculating aquarium system in the Atmosphere and Ocean Research Institute, the University of Tokyo. The aquarium system was controlled at 26 ?C with a photoperiod of 14h/10h light/dark cycle. Fish was fed daily with freshly hatched brine shrimp. Individuals (1.0 - 1.2 g body weight) were randomly divided into nine experimental tanks. Medaka were transferred to 50% seawater. In sampling, the experimental fish was anaesthetized in 0.1% tricaine methanesulfonate (MS-222) and further sacrificed by spinal transection. Intestine was immediately dissected, snap-frozen in liquid nitrogen, and stored at -80 ?C until use. Total RNA was extracted from the intestine samples using Isogen (Nippon Gene, Toyama) according to the manufacturer protocols. Poly(A) RNA was isolated from the total RNA according to the manufacturer protocols. Strain C57BL/6J SAMD00013898 RNA-Seq of medaka digestive tract 3 hour after transfer from freshwater to seawater, replicate 4 10090 Mus musculus Medaka were bred and growth in a freshwater recirculating aquarium system in the Atmosphere and Ocean Research Institute, the University of Tokyo. The aquarium system was controlled at 26 ?C with a photoperiod of 14h/10h light/dark cycle. Fish was fed daily with freshly hatched brine shrimp. Individuals (1.0 - 1.2 g body weight) were randomly divided into nine experimental tanks. Medaka were transferred to 50% seawater. In sampling, the experimental fish was anaesthetized in 0.1% tricaine methanesulfonate (MS-222) and further sacrificed by spinal transection. Intestine was immediately dissected, snap-frozen in liquid nitrogen, and stored at -80 ?C until use. Total RNA was extracted from the intestine samples using Isogen (Nippon Gene, Toyama) according to the manufacturer protocols. Poly(A) RNA was isolated from the total RNA according to the manufacturer protocols. Strain C57BL/6J SAMD00013906 RNA-Seq of medaka digestive tract 3 hour after transfer from freshwater to seawater, replicate 5 10090 Mus musculus Medaka were bred and growth in a freshwater recirculating aquarium system in the Atmosphere and Ocean Research Institute, the University of Tokyo. The aquarium system was controlled at 26 ?C with a photoperiod of 14h/10h light/dark cycle. Fish was fed daily with freshly hatched brine shrimp. Individuals (1.0 - 1.2 g body weight) were randomly divided into nine experimental tanks. Medaka were transferred to 50% seawater. In sampling, the experimental fish was anaesthetized in 0.1% tricaine methanesulfonate (MS-222) and further sacrificed by spinal transection. Intestine was immediately dissected, snap-frozen in liquid nitrogen, and stored at -80 ?C until use. Total RNA was extracted from the intestine samples using Isogen (Nippon Gene, Toyama) according to the manufacturer protocols. Poly(A) RNA was isolated from the total RNA according to the manufacturer protocols. Strain C57BL/6J SAMD00013899 RNA-Seq of medaka digestive tract 1 day after transfer from freshwater to seawater, replicate 1 10090 Mus musculus Medaka were bred and growth in a freshwater recirculating aquarium system in the Atmosphere and Ocean Research Institute, the University of Tokyo. The aquarium system was controlled at 26 ?C with a photoperiod of 14h/10h light/dark cycle. Fish was fed daily with freshly hatched brine shrimp. Individuals (1.0 - 1.2 g body weight) were randomly divided into nine experimental tanks. Medaka were transferred to 50% seawater. In sampling, the experimental fish was anaesthetized in 0.1% tricaine methanesulfonate (MS-222) and further sacrificed by spinal transection. Intestine was immediately dissected, snap-frozen in liquid nitrogen, and stored at -80 ?C until use. Total RNA was extracted from the intestine samples using Isogen (Nippon Gene, Toyama) according to the manufacturer protocols. Poly(A) RNA was isolated from the total RNA according to the manufacturer protocols. Strain C57BL/6J SAMD00013886 RNA-Seq of medaka digestive tract 1 day after transfer from freshwater to seawater, replicate 2 10090 Mus musculus Medaka were bred and growth in a freshwater recirculating aquarium system in the Atmosphere and Ocean Research Institute, the University of Tokyo. The aquarium system was controlled at 26 ?C with a photoperiod of 14h/10h light/dark cycle. Fish was fed daily with freshly hatched brine shrimp. Individuals (1.0 - 1.2 g body weight) were randomly divided into nine experimental tanks. Medaka were transferred to 50% seawater. In sampling, the experimental fish was anaesthetized in 0.1% tricaine methanesulfonate (MS-222) and further sacrificed by spinal transection. Intestine was immediately dissected, snap-frozen in liquid nitrogen, and stored at -80 ?C until use. Total RNA was extracted from the intestine samples using Isogen (Nippon Gene, Toyama) according to the manufacturer protocols. Poly(A) RNA was isolated from the total RNA according to the manufacturer protocols. Strain C57BL/6J SAMD00013908 RNA-Seq of medaka digestive tract 1 day after transfer from freshwater to seawater, replicate 3 10090 Mus musculus Medaka were bred and growth in a freshwater recirculating aquarium system in the Atmosphere and Ocean Research Institute, the University of Tokyo. The aquarium system was controlled at 26 ?C with a photoperiod of 14h/10h light/dark cycle. Fish was fed daily with freshly hatched brine shrimp. Individuals (1.0 - 1.2 g body weight) were randomly divided into nine experimental tanks. Medaka were transferred to 50% seawater. In sampling, the experimental fish was anaesthetized in 0.1% tricaine methanesulfonate (MS-222) and further sacrificed by spinal transection. Intestine was immediately dissected, snap-frozen in liquid nitrogen, and stored at -80 ?C until use. Total RNA was extracted from the intestine samples using Isogen (Nippon Gene, Toyama) according to the manufacturer protocols. Poly(A) RNA was isolated from the total RNA according to the manufacturer protocols. Strain C57BL/6J SAMD00013897 RNA-Seq of medaka digestive tract 1 day after transfer from freshwater to seawater, replicate 4 10090 Mus musculus Medaka were bred and growth in a freshwater recirculating aquarium system in the Atmosphere and Ocean Research Institute, the University of Tokyo. The aquarium system was controlled at 26 ?C with a photoperiod of 14h/10h light/dark cycle. Fish was fed daily with freshly hatched brine shrimp. Individuals (1.0 - 1.2 g body weight) were randomly divided into nine experimental tanks. Medaka were transferred to 50% seawater. In sampling, the experimental fish was anaesthetized in 0.1% tricaine methanesulfonate (MS-222) and further sacrificed by spinal transection. Intestine was immediately dissected, snap-frozen in liquid nitrogen, and stored at -80 ?C until use. Total RNA was extracted from the intestine samples using Isogen (Nippon Gene, Toyama) according to the manufacturer protocols. Poly(A) RNA was isolated from the total RNA according to the manufacturer protocols. Strain C57BL/6J SAMD00013887 RNA-Seq of medaka digestive tract 1 day after transfer from freshwater to seawater, replicate 5 10090 Mus musculus Medaka were bred and growth in a freshwater recirculating aquarium system in the Atmosphere and Ocean Research Institute, the University of Tokyo. The aquarium system was controlled at 26 ?C with a photoperiod of 14h/10h light/dark cycle. Fish was fed daily with freshly hatched brine shrimp. Individuals (1.0 - 1.2 g body weight) were randomly divided into nine experimental tanks. Medaka were transferred to 50% seawater. In sampling, the experimental fish was anaesthetized in 0.1% tricaine methanesulfonate (MS-222) and further sacrificed by spinal transection. Intestine was immediately dissected, snap-frozen in liquid nitrogen, and stored at -80 ?C until use. Total RNA was extracted from the intestine samples using Isogen (Nippon Gene, Toyama) according to the manufacturer protocols. Poly(A) RNA was isolated from the total RNA according to the manufacturer protocols. Strain C57BL/6J SAMD00013909 RNA-Seq of medaka digestive tract 7 day after transfer from freshwater to seawater, replicate 1 10090 Mus musculus Medaka were bred and growth in a freshwater recirculating aquarium system in the Atmosphere and Ocean Research Institute, the University of Tokyo. The aquarium system was controlled at 26 ?C with a photoperiod of 14h/10h light/dark cycle. Fish was fed daily with freshly hatched brine shrimp. Individuals (1.0 - 1.2 g body weight) were randomly divided into nine experimental tanks. Medaka were transferred to 50% seawater. In sampling, the experimental fish was anaesthetized in 0.1% tricaine methanesulfonate (MS-222) and further sacrificed by spinal transection. Intestine was immediately dissected, snap-frozen in liquid nitrogen, and stored at -80 ?C until use. Total RNA was extracted from the intestine samples using Isogen (Nippon Gene, Toyama) according to the manufacturer protocols. Poly(A) RNA was isolated from the total RNA according to the manufacturer protocols. Strain C57BL/6J SAMD00013894 RNA-Seq of medaka digestive tract 7 day after transfer from freshwater to seawater, replicate 2 10090 Mus musculus Medaka were bred and growth in a freshwater recirculating aquarium system in the Atmosphere and Ocean Research Institute, the University of Tokyo. The aquarium system was controlled at 26 ?C with a photoperiod of 14h/10h light/dark cycle. Fish was fed daily with freshly hatched brine shrimp. Individuals (1.0 - 1.2 g body weight) were randomly divided into nine experimental tanks. Medaka were transferred to 50% seawater. In sampling, the experimental fish was anaesthetized in 0.1% tricaine methanesulfonate (MS-222) and further sacrificed by spinal transection. Intestine was immediately dissected, snap-frozen in liquid nitrogen, and stored at -80 ?C until use. Total RNA was extracted from the intestine samples using Isogen (Nippon Gene, Toyama) according to the manufacturer protocols. Poly(A) RNA was isolated from the total RNA according to the manufacturer protocols. Strain C57BL/6J SAMD00013901 RNA-Seq of medaka digestive tract 7 day after transfer from freshwater to seawater, replicate 3 10090 Mus musculus Medaka were bred and growth in a freshwater recirculating aquarium system in the Atmosphere and Ocean Research Institute, the University of Tokyo. The aquarium system was controlled at 26 ?C with a photoperiod of 14h/10h light/dark cycle. Fish was fed daily with freshly hatched brine shrimp. Individuals (1.0 - 1.2 g body weight) were randomly divided into nine experimental tanks. Medaka were transferred to 50% seawater. In sampling, the experimental fish was anaesthetized in 0.1% tricaine methanesulfonate (MS-222) and further sacrificed by spinal transection. Intestine was immediately dissected, snap-frozen in liquid nitrogen, and stored at -80 ?C until use. Total RNA was extracted from the intestine samples using Isogen (Nippon Gene, Toyama) according to the manufacturer protocols. Poly(A) RNA was isolated from the total RNA according to the manufacturer protocols. Strain C57BL/6J SAMD00013910 RNA-Seq of medaka digestive tract 7 day after transfer from freshwater to seawater, replicate 4 10090 Mus musculus Medaka were bred and growth in a freshwater recirculating aquarium system in the Atmosphere and Ocean Research Institute, the University of Tokyo. The aquarium system was controlled at 26 ?C with a photoperiod of 14h/10h light/dark cycle. Fish was fed daily with freshly hatched brine shrimp. Individuals (1.0 - 1.2 g body weight) were randomly divided into nine experimental tanks. Medaka were transferred to 50% seawater. In sampling, the experimental fish was anaesthetized in 0.1% tricaine methanesulfonate (MS-222) and further sacrificed by spinal transection. Intestine was immediately dissected, snap-frozen in liquid nitrogen, and stored at -80 ?C until use. Total RNA was extracted from the intestine samples using Isogen (Nippon Gene, Toyama) according to the manufacturer protocols. Poly(A) RNA was isolated from the total RNA according to the manufacturer protocols. Strain C57BL/6J SAMD00013889 RNA-Seq of medaka digestive tract 7 day after transfer from freshwater to seawater, replicate 5 10090 Mus musculus Medaka were bred and growth in a freshwater recirculating aquarium system in the Atmosphere and Ocean Research Institute, the University of Tokyo. The aquarium system was controlled at 26 ?C with a photoperiod of 14h/10h light/dark cycle. Fish was fed daily with freshly hatched brine shrimp. Individuals (1.0 - 1.2 g body weight) were randomly divided into nine experimental tanks. Medaka were transferred to 50% seawater. In sampling, the experimental fish was anaesthetized in 0.1% tricaine methanesulfonate (MS-222) and further sacrificed by spinal transection. Intestine was immediately dissected, snap-frozen in liquid nitrogen, and stored at -80 ?C until use. Total RNA was extracted from the intestine samples using Isogen (Nippon Gene, Toyama) according to the manufacturer protocols. Poly(A) RNA was isolated from the total RNA according to the manufacturer protocols. Strain C57BL/6J