SAMD00000810 100573 Ficus septica Saplings of F. septica naturally grown in a forest at the University of the Ryukyus (26.25 N 127.77 E) were transplanted into 30-L pots (one sapling per pot). The saplings were maintained under ambient conditions and irrigated 2-3 times per week before use. Only healthy, mature leaves of F. septica were selected for analysis. Variability of isoprene emission and photosynthesis of F. septica in response to temperature was examined using a leaf chamber, a real time isoprene analyser, and a portable photosynthesis analyser in a phytotron, as described previously (Oku et al., Chemosphere 95, 268-273, 2014). Light (300 ?mol?m-2?s-1 of PPFD) and dark cycle was maintained at 12-h:12-h (L:D). The temperature regime to which the saplings were exposed was 30-24-18-12-30-30 ?C for over six days (i.e. temperature was lowered from 30 ?C to 12 ?C in 6 ?C daily increments over the first four days and then elevated again to 30 ?C in the latter two days). Hereafter, the first four days and latter two days are referred to as Day -4 to -1 and Day 1 to 2, respectively. Temperatures were changed when switching from the dark to light phase. Gene expression was analysed using a 5500xl SOLiD sequencer (Life Technologies, CA, USA). For this analysis, a leaf from one particular sapling was harvested at each sample time point. Samples were collected during the day at time points of 0.5 h and 6 h on Days 1 and 2, for a total of four time points. SAMD00000808 100573 Ficus septica Saplings of F. septica naturally grown in a forest at the University of the Ryukyus (26.25 N 127.77 E) were transplanted into 30-L pots (one sapling per pot). The saplings were maintained under ambient conditions and irrigated 2-3 times per week before use. Only healthy, mature leaves of F. septica were selected for analysis. Variability of isoprene emission and photosynthesis of F. septica in response to temperature was examined using a leaf chamber, a real time isoprene analyser, and a portable photosynthesis analyser in a phytotron, as described previously (Oku et al., Chemosphere 95, 268-273, 2014). Light (300 ?mol?m-2?s-1 of PPFD) and dark cycle was maintained at 12-h:12-h (L:D). The temperature regime to which the saplings were exposed was 30-24-18-12-30-30 ?C for over six days (i.e. temperature was lowered from 30 ?C to 12 ?C in 6 ?C daily increments over the first four days and then elevated again to 30 ?C in the latter two days). Hereafter, the first four days and latter two days are referred to as Day -4 to -1 and Day 1 to 2, respectively. Temperatures were changed when switching from the dark to light phase. Gene expression was analysed using a 5500xl SOLiD sequencer (Life Technologies, CA, USA). For this analysis, a leaf from one particular sapling was harvested at each sample time point. Samples were collected during the day at time points of 0.5 h and 6 h on Days 1 and 2, for a total of four time points. SAMD00000807 100573 Ficus septica Saplings of F. septica naturally grown in a forest at the University of the Ryukyus (26.25 N 127.77 E) were transplanted into 30-L pots (one sapling per pot). The saplings were maintained under ambient conditions and irrigated 2-3 times per week before use. Only healthy, mature leaves of F. septica were selected for analysis. Variability of isoprene emission and photosynthesis of F. septica in response to temperature was examined using a leaf chamber, a real time isoprene analyser, and a portable photosynthesis analyser in a phytotron, as described previously (Oku et al., Chemosphere 95, 268-273, 2014). Light (300 ?mol?m-2?s-1 of PPFD) and dark cycle was maintained at 12-h:12-h (L:D). The temperature regime to which the saplings were exposed was 30-24-18-12-30-30 ?C for over six days (i.e. temperature was lowered from 30 ?C to 12 ?C in 6 ?C daily increments over the first four days and then elevated again to 30 ?C in the latter two days). Hereafter, the first four days and latter two days are referred to as Day -4 to -1 and Day 1 to 2, respectively. Temperatures were changed when switching from the dark to light phase. Gene expression was analysed using a 5500xl SOLiD sequencer (Life Technologies, CA, USA). For this analysis, a leaf from one particular sapling was harvested at each sample time point. Samples were collected during the day at time points of 0.5 h and 6 h on Days 1 and 2, for a total of four time points. SAMD00000809 100573 Ficus septica Saplings of F. septica naturally grown in a forest at the University of the Ryukyus (26.25 N 127.77 E) were transplanted into 30-L pots (one sapling per pot). The saplings were maintained under ambient conditions and irrigated 2-3 times per week before use. Only healthy, mature leaves of F. septica were selected for analysis. Variability of isoprene emission and photosynthesis of F. septica in response to temperature was examined using a leaf chamber, a real time isoprene analyser, and a portable photosynthesis analyser in a phytotron, as described previously (Oku et al., Chemosphere 95, 268-273, 2014). Light (300 ?mol?m-2?s-1 of PPFD) and dark cycle was maintained at 12-h:12-h (L:D). The temperature regime to which the saplings were exposed was 30-24-18-12-30-30 ?C for over six days (i.e. temperature was lowered from 30 ?C to 12 ?C in 6 ?C daily increments over the first four days and then elevated again to 30 ?C in the latter two days). Hereafter, the first four days and latter two days are referred to as Day -4 to -1 and Day 1 to 2, respectively. Temperatures were changed when switching from the dark to light phase. Gene expression was analysed using a 5500xl SOLiD sequencer (Life Technologies, CA, USA). For this analysis, a leaf from one particular sapling was harvested at each sample time point. Samples were collected during the day at time points of 0.5 h and 6 h on Days 1 and 2, for a total of four time points. SAMD00000811 100573 Ficus septica Saplings of Ficus septica naturally grown in a forest at the University of the Ryukyus (26.25 N 127.77 E) were transplanted into 30-L pots (one sapling per pot). Leaves sampled from a saplings of F. septica at various times of day and pooled to construct a cDNA library.