SAMD00009818 9690 Homo sapiens This sample is total RNA in Hela TO cells.HeLa TO cells (Clontech) were grown in Dulbecco's modified Eagle’s medium (DMEM), supplemented with10% fetal bovine serum (FBS) and antibiotics at 37C in a humidified incubator with 5% CO2. SAMD00009817 9690 Homo sapiens This sample is total RNA in the siCTRL-depleted Hela TO cells.HeLa TO cells (Clontech) were grown in Dulbecco's modified Eagle’s medium (DMEM), supplemented with10% fetal bovine serum (FBS) and antibiotics at 37C in a humidified incubator with 5% CO2.The siCTRL were transfected into cells using Lipofectamine RNAiMAX (Invitrogen) according to the manufacturer’s instructions. Briefly, siCTRL duplexes (final concentration 10 nM) and cells were harvested 48 hr posttransfection. SAMD00009822 9690 Homo sapiens This sample is total RNA in the siCTRL-depleted and poly I:C stimulated Hela TO cells.HeLa TO cells (Clontech) were grown in Dulbecco's modified Eagle’s medium (DMEM), supplemented with10% fetal bovine serum (FBS) and antibiotics at 37C in a humidified incubator with 5% CO2.According to the manufacturer’s protocol, cells were transfected with poly I:C using Lipofectamine 2000 (Invitrogen). For poly I:C stimulation, cells were seeded into 12-well cell culture plates followed by transfection using the indicated amounts of poly I:C and Lipofectamine 2000. The siCTRL were transfected into cells using Lipofectamine RNAiMAX (Invitrogen) according to the manufacturer’s instructions. Briefly, siCTRL duplexes (final concentration 10 nM) and cells were harvested 48 hr posttransfection. SAMD00009815 9690 Homo sapiens This sample is total RNA in the siNEAT1-depleted Hela TO cells.HeLa TO cells (Clontech) were grown in Dulbecco's modified Eagle’s medium (DMEM), supplemented with10% fetal bovine serum (FBS) and antibiotics at 37C in a humidified incubator with 5% CO2. The siNEAT1 were transfected into cells using Lipofectamine RNAiMAX (Invitrogen) according to the manufacturer’s instructions. Briefly, siNEAT1 duplexes (final concentration 10 nM) and cells were harvested 48 hr posttransfection. SAMD00009820 9690 Homo sapiens This sample is total RNA in the siNEAT1-depleted and poly I:C stimulated Hela TO cells.HeLa TO cells (Clontech) were grown in Dulbecco's modified Eagle’s medium (DMEM), supplemented with10% fetal bovine serum (FBS) and antibiotics at 37C in a humidified incubator with 5% CO2. According to the manufacturer’s protocol, cells were transfected with poly I:C using Lipofectamine 2000 (Invitrogen). For poly I:C stimulation, cells were seeded into 12-well cell culture plates followed by transfection using the indicated amounts of poly I:C and Lipofectamine 2000. The siNEAT1 were transfected into cells using Lipofectamine RNAiMAX (Invitrogen) according to the manufacturer’s instructions. Briefly, siNEAT1 duplexes (final concentration 10 nM) and cells were harvested 48 hr posttransfection. SAMD00009821 9690 Homo sapiens This sample is total RNA in the siCTRL-depleted Hela TO cells.HeLa TO cells (Clontech) were grown in Dulbecco's modified Eagle’s medium (DMEM), supplemented with 10% fetal bovine serum (FBS) and antibiotics at 37C in a humidified incubator with 5% CO2.The siCTRL were transfected into cells using Lipofectamine RNAiMAX (Invitrogen) according to the manufacturer’s instructions. Briefly, siCTRL duplexes (final concentration 10 nM) and cells were harvested 48 hr posttransfection. SAMD00009816 9690 Homo sapiens This sample is total RNA in the siPSF-3-depleted Hela TO cells.HeLa TO cells (Clontech) were grown in Dulbecco's modified Eagle’s medium (DMEM), supplemented with 10% fetal bovine serum (FBS) and antibiotics at 37C in a humidified incubator with 5% CO2.The siPSF-3 were transfected into cells using Lipofectamine RNAiMAX (Invitrogen) according to the manufacturer’s instructions. Briefly, siPSF-3 duplexes (final concentration 10 nM) and cells were harvested 48 hr posttransfection. SAMD00009819 9690 Homo sapiens This sample is total RNA in the siPSF-4-depleted Hela TO cells.HeLa TO cells (Clontech) were grown in Dulbecco's modified Eagle’s medium (DMEM), supplemented with 10% fetal bovine serum (FBS) and antibiotics at 37C in a humidified incubator with 5% CO2.The siPSF-4 were transfected into cells using Lipofectamine RNAiMAX (Invitrogen) according to the manufacturer’s instructions. Briefly, siPSF-4 duplexes (final concentration 10 nM) and cells were harvested 48 hr posttransfection.