PRJDB1666 we attempted to distinguish RNA-seq reads of the parasite from those of the host by using transcript information of species close to host and parasite. For RNA-seq analysis, sequencing library of parasitic tissue was prepared using RNA obtained from parasitic site of Cuscuta japonica to its host, Impatiens balsamina. 1 NIL PRJDB1666 Cuscuta japonica, one of the parasitic plants, coils around the host stem and penetrates host tissue and acquires water and nutrients. In the parasitic sites, cells from both plants are tightly connected each other. Hence it is difficult to analyze only parasite in the parasitic tissue. In this study, to overcome this difficulty, we attempted to distinguish RNA-seq reads of the parasite from those of the host by using transcript information of species close to C. japonica. For RNA-seq analysis, sequencing library of parasitic tissue was prepared using RNA obtained from parasitic site of C. japonica to its host, Impatiens balsamina. To distinguish reads of C. japonica from those of its host, we used sequence information of RNA-seq reads of close relative, Cuscuta genus and sequence information of Convoluvulaceae resisted in NCBI nt database. Using this approach, we distinguished reads of C. japonica from its host, and finally assembled them into contigs. 1 NIL