SAMD00019994 511145 Escherichia coli str. K-12 substr. MG1655 description The E. coli strain used was BHY173, a MG1655 derivative with mutations in restriction enzyme genes (genotype: MG1655 delta(mrr-hsdRMS-mcrB) deltamcrA). BHY187: BHY173(pACYC184deltatet). This strain was grown in Lennox LB up to mid-exponential phase (O.D.600 =0.5) in two different batches. Then cells were harvested for RNA extraction. Total RNA was extracted from cell pellets using PureLink RNA mini kit (Ambion). rRNA was depleted using Ribo-Zero rRNA Removal kit (Gram-negative bacteria)(Epicentre). cDNA library for mRNA-seq was constructed using TruSeq RNA Sample Prep kit (Illumina). The indexed cDNA libraries were pooled and sequenced in HiSeq 2000 platform (Illumina). bioproject_id PRJDB3140 sample_name BHY187-1 strain BHY173 sample_title BHY187-1 genotype MG1655 delta(mrr-hsdRMS-mcrB) deltamcrA SAMD00019995 511145 Escherichia coli str. K-12 substr. MG1655 description The E. coli strain used was BHY173, a MG1655 derivative with mutations in restriction enzyme genes (genotype: MG1655 delta(mrr-hsdRMS-mcrB) deltamcrA). BHY188: BHY173(pACYC184deltatet::dcm). This strain was grown in Lennox LB up to mid-exponential phase (O.D.600 =0.5) in two different batches. Then cells were harvested for RNA extraction. Total RNA was extracted from cell pellets using PureLink RNA mini kit (Ambion). rRNA was depleted using Ribo-Zero rRNA Removal kit (Gram-negative bacteria)(Epicentre). cDNA library for mRNA-seq was constructed using TruSeq RNA Sample Prep kit (Illumina). The indexed cDNA libraries were pooled and sequenced in HiSeq 2000 platform (Illumina). bioproject_id PRJDB3140 sample_name BHY188-1 strain BHY173 sample_title BHY188-1 genotype MG1655 delta(mrr-hsdRMS-mcrB) deltamcrA SAMD00019996 511145 Escherichia coli str. K-12 substr. MG1655 description The E. coli strain used was BHY173, a MG1655 derivative with mutations in restriction enzyme genes (genotype: MG1655 delta(mrr-hsdRMS-mcrB) deltamcrA). BHY189: BHY173(pACYC184deltatet::ecoRIM). This strain was grown in Lennox LB up to mid-exponential phase (O.D.600 =0.5) in two different batches. Then cells were harvested for RNA extraction. Total RNA was extracted from cell pellets using PureLink RNA mini kit (Ambion). rRNA was depleted using Ribo-Zero rRNA Removal kit (Gram-negative bacteria)(Epicentre). cDNA library for mRNA-seq was constructed using TruSeq RNA Sample Prep kit (Illumina). The indexed cDNA libraries were pooled and sequenced in HiSeq 2000 platform (Illumina). bioproject_id PRJDB3140 sample_name BHY189-1 strain BHY173 sample_title BHY189-1 genotype MG1655 delta(mrr-hsdRMS-mcrB) deltamcrA SAMD00019997 511145 Escherichia coli str. K-12 substr. MG1655 description The E. coli strain used was BHY173, a MG1655 derivative with mutations in restriction enzyme genes (genotype: MG1655 delta(mrr-hsdRMS-mcrB) deltamcrA). BHY193: BHY173(pACYC184deltatet::ecoP15Imod). This strain was grown in Lennox LB up to mid-exponential phase (O.D.600 =0.5) in two different batches. Then cells were harvested for RNA extraction. Total RNA was extracted from cell pellets using PureLink RNA mini kit (Ambion). rRNA was depleted using Ribo-Zero rRNA Removal kit (Gram-negative bacteria)(Epicentre). cDNA library for mRNA-seq was constructed using TruSeq RNA Sample Prep kit (Illumina). The indexed cDNA libraries were pooled and sequenced in HiSeq 2000 platform (Illumina). bioproject_id PRJDB3140 sample_name BHY193-1 strain BHY173 sample_title BHY193-1 genotype MG1655 delta(mrr-hsdRMS-mcrB) deltamcrA SAMD00019998 511145 Escherichia coli str. K-12 substr. MG1655 description The E. coli strain used was BHY173, a MG1655 derivative with mutations in restriction enzyme genes (genotype: MG1655 delta(mrr-hsdRMS-mcrB) deltamcrA). BHY212: BHY173(pACYC184deltatet::ecoP1Imod). This strain was grown in Lennox LB up to mid-exponential phase (O.D.600 =0.5) in two different batches. Then cells were harvested for RNA extraction. Total RNA was extracted from cell pellets using PureLink RNA mini kit (Ambion). rRNA was depleted using Ribo-Zero rRNA Removal kit (Gram-negative bacteria)(Epicentre). cDNA library for mRNA-seq was constructed using TruSeq RNA Sample Prep kit (Illumina). The indexed cDNA libraries were pooled and sequenced in HiSeq 2000 platform (Illumina). bioproject_id PRJDB3140 sample_name BHY212-1 strain BHY173 sample_title BHY212-1 genotype MG1655 delta(mrr-hsdRMS-mcrB) deltamcrA