PRJDB3092 SAMD00019672 TSS-lib_Mfa_zoox_1st EST TRANSCRIPTOMIC CAGE Total RNA was treated with 2.5 U of BAP (TaKaRa) at 37 ?C for 1 h and 40 U of TAP (Ambion) at 37 ?C for 1 h. BAP-TAP-treated RNAs were ligated with the RNA oligonucleotide. After DNAse treatment, poly(A)-containing RNA was selected and the first strand cDNA was synthesized with random hexamer primer. The PCR fragments were size-fractionated. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB3092 SAMD00019672 TSS-lib_Mfa_zoox_2nd EST TRANSCRIPTOMIC CAGE Total RNA was treated with 2.5 U of BAP (TaKaRa) at 37 ?C for 1 h and 40 U of TAP (Ambion) at 37 ?C for 1 h. BAP-TAP-treated RNAs were ligated with the RNA oligonucleotide. After DNAse treatment, poly(A)-containing RNA was selected and the first strand cDNA was synthesized with random hexamer primer. The PCR fragments were size-fractionated. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000