SAMD00021017 6239 Caenorhabditis elegans description The worm strain used was KH1683. This strain is mutant worm. The genotype is asd-2(yb1540) I. Total RNAs were extracted from synchronized L1 worms by using RNeasy Midi kit (Qiagen). Poly (A)+ RNAs were repeatedly purified with FastTrack MAG (Invitrogen), fragmented in 1~ Fragmentation buffer (Ambion) and reverse transcribed using random hexamers and Primescript II (Takara). Second strand cDNAs were synthesized by using RNase H (Invitrogen) and DNA polymerase I (Invitrogen). Deep sequencing was performed by utilizing Genome Analyzer II (Illumina) following standard protocols. bioproject_id PRJDB2743 dev_stage L1 sample_name KH1683 L1 strain KH1683 sample_title asd-2 L1 [RNAseq] genotype asd-2(yb1540) I