SAMD00022899 9606 Homo sapiens description Cells from one 10 cm dish (~1 x 107) of HEK293T cells grown to 70-80% confluence were used for each immunoprecipitation. The cells were cross-linked with 2 mM DSG Crosslinker in PBS for 30 min and then 1% formaldehyde in PBS for 20 min at room temperature. Then the cells were resuspended and lysed in lysis buffer (0.5% SDS, 10mM EDTA, 150 mM NaCl, 50 mM Tris-HCl pH 8.0), and they were sonicated with a Bioruptor Sonicator (Diagenode, Denville, NJ) for 30 times for 30 seconds at the maximum power setting to generate DNA fragments of ~150-500 bps. Sonicated chromatin was incubated at 4C overnight with 10 ug of anti- ICE1 (raised against bacterially expressed, His-tagged recombinant proteins of ICE1 1865-2266 aa). Crosslinking was reversed by overnight incubation at 65C. Immunoprecipitated DNA were treated with RNase A and Proteinase K by incubation at 45C. DNA was purified using the QIAquick MinElute PCR purification kit (28006, Qiagen). bioproject_id PRJDB3302 cell_line HEK293T sample_name KIAA0947 sample_title HEK293T_ICE1_ChIPseq SAMD00022900 9606 Homo sapiens description Cells from one 10 cm dish (~1 x 107) of HEK293T cells treated with MED26 siRNA (s18074, Ambion/Life Technologies) were used for each immunoprecipitation. The cells were cross-linked with 2 mM DSG Crosslinker in PBS for 30 min and then 1% formaldehyde in PBS for 20 min at room temperature. Then the cells were resuspended and lysed in lysis buffer (0.5% SDS, 10mM EDTA, 150 mM NaCl, 50 mM Tris-HCl pH 8.0), and they were sonicated with a Bioruptor Sonicator (Diagenode, Denville, NJ) for 30 times for 30 seconds at the maximum power setting to generate DNA fragments of ~150-500 bps. Sonicated chromatin was incubated at 4C overnight with 10 ug of anti- ICE1 (raised against bacterially expressed, His-tagged recombinant proteins of ICE1 1865-2266 aa). Crosslinking was reversed by overnight incubation at 65C. Immunoprecipitated DNA were treated with RNase A and Proteinase K by incubation at 45C. DNA was purified using the QIAquick MinElute PCR purification kit (28006, Qiagen). bioproject_id PRJDB3302 cell_line HEK293T sample_name MED26 KD_ICE sample_title HEK293T_MED26_KD_ICE1_ChIPseq SAMD00022901 9606 Homo sapiens description Cells from one 10 cm dish (~1 x 107) of HEK293T cells treated with MED26 siRNA (s18074, Ambion/Life Technologies) were used for each immunoprecipitation. The cells were cross-linked with 2 mM DSG Crosslinker in PBS for 30 min and then 1% formaldehyde in PBS for 20 min at room temperature. Then the cells were resuspended and lysed in lysis buffer (0.5% SDS, 10mM EDTA, 150 mM NaCl, 50 mM Tris-HCl pH 8.0), and they were sonicated with a Bioruptor Sonicator (Diagenode, Denville, NJ) for 30 times for 30 seconds at the maximum power setting to generate DNA fragments of ~150-500 bps. Crosslinking was reversed by overnight incubation at 65C. Input DNA were treated with RNase A and Proteinase K by incubation at 45C. DNA was purified using the QIAquick PCR purification kit (28106, Qiagen). bioproject_id PRJDB3302 cell_line HEK293T sample_name MED26 KD_Input sample_title HEK293T_MED26_KD_input_ChIPseq SAMD00022902 9606 Homo sapiens description Cells from one 10 cm dish (~1 x 107) of HEK293T cells treated with non-targeting siRNA (D-001206-14, Dharmacon) were used for each immunoprecipitation. The cells were cross-linked with 2 mM DSG Crosslinker in PBS for 30 min and then 1% formaldehyde in PBS for 20 min at room temperature. Then the cells were resuspended and lysed in lysis buffer (0.5% SDS, 10mM EDTA, 150 mM NaCl, 50 mM Tris-HCl pH 8.0), and they were sonicated with a Bioruptor Sonicator (Diagenode, Denville, NJ) for 30 times for 30 seconds at the maximum power setting to generate DNA fragments of ~150-500 bps. Sonicated chromatin was incubated at 4C overnight with 10 ug of anti- ICE1 (raised against bacterially expressed, His-tagged recombinant proteins of ICE1 1865-2266 aa). Crosslinking was reversed by overnight incubation at 65C. Immunoprecipitated DNA were treated with RNase A and Proteinase K by incubation at 45C. DNA was purified using the QIAquick MinElute PCR purification kit (28006, Qiagen). bioproject_id PRJDB3302 cell_line HEK293T sample_name NT_ICE1 IP sample_title HEK293T_NT_ICE1_ChIPseq SAMD00022903 9606 Homo sapiens description Cells from one 10 cm dish (~1 x 107) of HEK293T cells treated with non-targeting siRNA (D-001206-14, Dharmacon) were used for each immunoprecipitation. The cells were cross-linked with 2 mM DSG Crosslinker in PBS for 30 min and then 1% formaldehyde in PBS for 20 min at room temperature. Then the cells were resuspended and lysed in lysis buffer (0.5% SDS, 10mM EDTA, 150 mM NaCl, 50 mM Tris-HCl pH 8.0), and they were sonicated with a Bioruptor Sonicator (Diagenode, Denville, NJ) for 30 times for 30 seconds at the maximum power setting to generate DNA fragments of ~150-500 bps. Crosslinking was reversed by overnight incubation at 65C. Input DNA were treated with RNase A and Proteinase K by incubation at 45C. DNA was purified using the QIAquick PCR purification kit (28106, Qiagen). bioproject_id PRJDB3302 cell_line HEK293T sample_name NT_Input sample_title HEK293T_NT_input_ChIPseq SAMD00022904 9606 Homo sapiens description Cells from one 10 cm dish (~1 x 107) of HEK293T cells grown to 70-80% confluence were used for each immunoprecipitation. The cells were cross-linked with 2 mM DSG Crosslinker in PBS for 30 min and then 1% formaldehyde in PBS for 20 min at room temperature. Then the cells were resuspended and lysed in lysis buffer (0.5% SDS, 10mM EDTA, 150 mM NaCl, 50 mM Tris-HCl pH 8.0), and they were sonicated with a Bioruptor Sonicator (Diagenode, Denville, NJ) for 30 times for 30 seconds at the maximum power setting to generate DNA fragments of ~150-500 bps. Sonicated chromatin was incubated at 4C overnight with 10 ug of anti-TAF7 (SQ-8, sc-101167, Santa cruz). Crosslinking was reversed by overnight incubation at 65C. Immunoprecipitated DNA were treated with RNase A and Proteinase K by incubation at 45C. DNA was purified using the QIAquick MinElute PCR purification kit (28006, Qiagen). bioproject_id PRJDB3302 cell_line HEK293T sample_name TAF7 sample_title HEK293T_TAF7_ChIPseq SAMD00022905 9606 Homo sapiens description Cells from one 10 cm dish (~1 x 107) of HEK293T cells grown to 70-80% confluence were used for each immunoprecipitation. The cells were cross-linked with 2 mM DSG Crosslinker in PBS for 30 min and then 1% formaldehyde in PBS for 20 min at room temperature. Then the cells were resuspended and lysed in lysis buffer (0.5% SDS, 10mM EDTA, 150 mM NaCl, 50 mM Tris-HCl pH 8.0), and they were sonicated with a Bioruptor Sonicator (Diagenode, Denville, NJ) for 30 times for 30 seconds at the maximum power setting to generate DNA fragments of ~150-500 bps. Crosslinking was reversed by overnight incubation at 65C. Input DNA were treated with RNase A and Proteinase K by incubation at 45C. DNA was purified using the QIAquick PCR purification kit (28106, Qiagen). bioproject_id PRJDB3302 cell_line HEK293T sample_name input sample_title HEK293T_input_ChIPseq