PRJDB3275 LATS2 knockout causes PRC2 dysregulation and global H3K27me3 loss in HeLa-S3 We report that knockout of LATS2 gene caused up-regulation of genes which are repressed by PRC2 partially independent of canonical hippo-YAP/TAZ signaling cascade in HeLa-S3 using both microarray and RNA-sequencing. LATS2 KO dependent down-regulation of PRC2 caused global H3K27me3 reduction and dysregulation of whole epigenetic integrity. ChIP-seq analysis also revealed that LATS2 dependent H3K27me3-mark was enriched in differentiation processes, especially neurogenesis. LATS2 knockout causes PRC2 dysregulation and global H3K27me3 loss in HeLa-S3 bioproject PRJDB3275 PRJDB3275 true We report that knockout of LATS2 gene caused up-regulation of genes which are repressed by PRC2 partially independent of canonical hippo-YAP/TAZ signaling cascade in HeLa-S3 using both microarray and RNA-sequencing. LATS2 KO dependent down-regulation of PRC2 caused global H3K27me3 reduction and dysregulation of whole epigenetic integrity. ChIP-seq analysis also revealed that LATS2 dependent H3K27me3-mark was enriched in differentiation processes, especially neurogenesis.