PRJDB3376 SAMD00024373 siANGPTL2 RNA-Seq TRANSCRIPTOMIC RANDOM MDA-MB231 cells were cultured in Leibovitz's L15 (Wako, Osaka, Japan) medium supplemented with 10% FCS, under 100% air without CO2, and transfected with siANGPTL2 vectors using Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA) according to the manufacturer's protocol. The cells were lysed in Trizol reagent (Invitrogen) and total RNA was extracted according to the manufacturer's protocol. Sequencing sample libraries were prepared using a TruSeq RNA Sample Prep Kit (Illumina, San Diego, CA, USA). 38 0 Application Read Forward 1 Illumina Genome Analyzer IIx PRJDB3376 SAMD00024374 LacZ RNA-Seq TRANSCRIPTOMIC RANDOM MDA-MB231 cells were cultured in Leibovitz's L15 (Wako, Osaka, Japan) medium supplemented with 10% FCS, under 100% air without CO2, and transfected with LacZ (control) vectors using Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA) according to the manufacturer's protocol. The cells were lysed in Trizol reagent (Invitrogen) and total RNA was extracted according to the manufacturer's protocol. Sequencing sample libraries were prepared using a TruSeq RNA Sample Prep Kit (Illumina, San Diego, CA, USA). 38 0 Application Read Forward 1 Illumina Genome Analyzer IIx