PRJDB4576 SAMD00047149 mir17AF1 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from 10 female abdomens of D. miranda. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy and further confirmed by PCR with Y chromosome specific primers after the extraction of genomic DNA from same individuals. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047149 mir17AF2 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from 10 female abdomens of D. miranda. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy and further confirmed by PCR with Y chromosome specific primers after the extraction of genomic DNA from same individuals. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047150 mir17AM1 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from 10 male abdomens of D. miranda. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy and further confirmed by PCR with Y chromosome specific primers after the extraction of genomic DNA from same individuals. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047150 mir17AM2 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from 10 male abdomens of D. miranda. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy and further confirmed by PCR with Y chromosome specific primers after the extraction of genomic DNA from same individuals. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047151 mir17AcM1 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from ~20 male accessory glands of D. miranda. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy and further confirmed by PCR with Y chromosome specific primers after the extraction of genomic DNA from same individuals. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047151 mir17AcM2 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from ~20 male accessory glands of D. miranda. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy and further confirmed by PCR with Y chromosome specific primers after the extraction of genomic DNA from same individuals. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047152 mir17AgF1 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from 10 female abdomens of D. miranda after removing gonads. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy and further confirmed by PCR with Y chromosome specific primers after the extraction of genomic DNA from same individuals. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047152 mir17AgF2 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from 10 female abdomens of D. miranda after removing gonads. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy and further confirmed by PCR with Y chromosome specific primers after the extraction of genomic DNA from same individuals. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047153 mir17AgM1 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from 10 male abdomens of D. miranda after removing gonads. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy and further confirmed by PCR with Y chromosome specific primers after the extraction of genomic DNA from same individuals. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047153 mir17AgM2 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from 10 male abdomens of D. miranda after removing gonads. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy and further confirmed by PCR with Y chromosome specific primers after the extraction of genomic DNA from same individuals. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047154 mir17DF1 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from ~20 female imaginal discs (except for genital imaginal disc) of D. miranda. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy and further confirmed by PCR with Y chromosome specific primers after the extraction of genomic DNA from same individuals. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047154 mir17DF2 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from ~20 female imaginal discs (except for genital imaginal disc) of D. miranda. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy and further confirmed by PCR with Y chromosome specific primers after the extraction of genomic DNA from same individuals. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047155 mir17DM1 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from ~20 male imaginal discs (except for genital imaginal disc) of D. miranda. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy and further confirmed by PCR with Y chromosome specific primers after the extraction of genomic DNA from same individuals. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047155 mir17DM2 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from ~20 male imaginal discs (except for genital imaginal disc) of D. miranda. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy and further confirmed by PCR with Y chromosome specific primers after the extraction of genomic DNA from same individuals. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047156 mir17HF1 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from ~20 female heads of D. miranda. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy and further confirmed by PCR with Y chromosome specific primers after the extraction of genomic DNA from same individuals. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047156 mir17HF2 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from ~20 female heads of D. miranda. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy and further confirmed by PCR with Y chromosome specific primers after the extraction of genomic DNA from same individuals. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047157 mir17HM1 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from ~20 male heads of D. miranda. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy and further confirmed by PCR with Y chromosome specific primers after the extraction of genomic DNA from same individuals. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047157 mir17HM2 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from ~20 male heads of D. miranda. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy and further confirmed by PCR with Y chromosome specific primers after the extraction of genomic DNA from same individuals. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047158 mir17LF1 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from 5 female 3rd instar larvae of D. miranda. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy and further confirmed by PCR with Y chromosome specific primers after the extraction of genomic DNA from same individuals. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047158 mir17LF2 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from 5 female 3rd instar larvae of D. miranda. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy and further confirmed by PCR with Y chromosome specific primers after the extraction of genomic DNA from same individuals. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047159 mir17LM1 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from 5 male 3rd instar larvae of D. miranda. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy and further confirmed by PCR with Y chromosome specific primers after the extraction of genomic DNA from same individuals. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047159 mir17LM2 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from 5 male 3rd instar larvae of D. miranda. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy and further confirmed by PCR with Y chromosome specific primers after the extraction of genomic DNA from same individuals. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047160 mir17LdF1 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from 5 female 3rd instar larvae of D. miranda after removing imaginal discs but containing genital imaginal disc. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy and further confirmed by PCR with Y chromosome specific primers after the extraction of genomic DNA from same individuals. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047160 mir17LdF2 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from 5 female 3rd instar larvae of D. miranda after removing imaginal discs but containing genital imaginal disc. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy and further confirmed by PCR with Y chromosome specific primers after the extraction of genomic DNA from same individuals. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047161 mir17LdM1 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from 5 male 3rd instar larvae of D. miranda after removing imaginal discs but containing genital imaginal disc. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy and further confirmed by PCR with Y chromosome specific primers after the extraction of genomic DNA from same individuals. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047161 mir17LdM2 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from 5 male 3rd instar larvae of D. miranda after removing imaginal discs but containing genital imaginal disc. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy and further confirmed by PCR with Y chromosome specific primers after the extraction of genomic DNA from same individuals. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047162 mir17OF1 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from ~20 female ovaries of D. miranda. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy and further confirmed by PCR with Y chromosome specific primers after the extraction of genomic DNA from same individuals. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047162 mir17OF2 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from ~20 female ovaries of D. miranda. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy and further confirmed by PCR with Y chromosome specific primers after the extraction of genomic DNA from same individuals. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047163 mir17PF1 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from 5 female pupae of D. miranda. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy and further confirmed by PCR with Y chromosome specific primers after the extraction of genomic DNA from same individuals. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047163 mir17PF2 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from 5 female pupae of D. miranda. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy and further confirmed by PCR with Y chromosome specific primers after the extraction of genomic DNA from same individuals. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047164 mir17PM1 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from 5 male pupae of D. miranda. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy and further confirmed by PCR with Y chromosome specific primers after the extraction of genomic DNA from same individuals. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047164 mir17PM2 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from 5 male pupae of D. miranda. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy and further confirmed by PCR with Y chromosome specific primers after the extraction of genomic DNA from same individuals. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047165 mir17TF1 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from 10 female thoraxes of D. miranda. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy and further confirmed by PCR with Y chromosome specific primers after the extraction of genomic DNA from same individuals. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047165 mir17TF2 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from 10 female thoraxes of D. miranda. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy and further confirmed by PCR with Y chromosome specific primers after the extraction of genomic DNA from same individuals. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047166 mir17TM1 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from 10 male thoraxes of D. miranda. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy and further confirmed by PCR with Y chromosome specific primers after the extraction of genomic DNA from same individuals. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047166 mir17TM2 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from 10 male thoraxes of D. miranda. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy and further confirmed by PCR with Y chromosome specific primers after the extraction of genomic DNA from same individuals. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047167 mir17TeM1 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from ~20 male testes of D. miranda. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy and further confirmed by PCR with Y chromosome specific primers after the extraction of genomic DNA from same individuals. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047167 mir17TeM2 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from ~20 male testes of D. miranda. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy and further confirmed by PCR with Y chromosome specific primers after the extraction of genomic DNA from same individuals. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047168 mir17WF1 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from 5 female adults of D. miranda. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy and further confirmed by PCR with Y chromosome specific primers after the extraction of genomic DNA from same individuals. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047168 mir17WF2 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from 5 female adults of D. miranda. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy and further confirmed by PCR with Y chromosome specific primers after the extraction of genomic DNA from same individuals. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047169 mir17WM1 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from 5 male adults of D. miranda. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy and further confirmed by PCR with Y chromosome specific primers after the extraction of genomic DNA from same individuals. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047169 mir17WM2 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from 5 male adults of D. miranda. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy and further confirmed by PCR with Y chromosome specific primers after the extraction of genomic DNA from same individuals. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047170 obsAF1 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from 10 female abdomens of D. obscura. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047170 obsAF2 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from 10 female abdomens of D. obscura. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047171 obsAM1 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from 10 male abdomens of D. obscura. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047171 obsAM2 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from 10 male abdomens of D. obscura. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047172 obsAcM1 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from ~20 male accessory glands of D. obscura. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047172 obsAcM2 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from ~20 male accessory glands of D. obscura. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047173 obsAgF1 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from 10 female abdomens of D. obscura after removing gonads. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047173 obsAgF2 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from 10 female abdomens of D. obscura after removing gonads. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047174 obsAgM1 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from 10 male abdomens of D. obscura after removing gonads. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047174 obsAgM2 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from 10 male abdomens of D. obscura after removing gonads. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047175 obsHF1 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from ~20 female heads of D. obscura. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047175 obsHF2 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from ~20 female heads of D. obscura. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047176 obsHM1 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from ~20 male heads of D. obscura. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047176 obsHM2 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from ~20 male heads of D. obscura. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047177 obsLF1 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from 5 female 3rd instar larvae of D. obscura. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047177 obsLF2 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from 5 female 3rd instar larvae of D. obscura. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047178 obsLM1 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from 5 male 3rd instar larvae of D. obscura. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047178 obsLM2 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from 5 male 3rd instar larvae of D. obscura. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047179 obsOF1 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from ~20 female ovaries of D. obscura. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047179 obsOF2 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from ~20 female ovaries of D. obscura. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047180 obsPF1 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from 5 female pupae of D. obscura. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047180 obsPF2 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from 5 female pupae of D. obscura. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047181 obsPM1 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from 5 male pupae of D. obscura. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047181 obsPM2 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from 5 male pupae of D. obscura. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047182 obsTF1 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from 10 female thoraxes of D. obscura. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047182 obsTF2 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from 10 female thoraxes of D. obscura. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047183 obsTM1 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from 10 male thoraxes of D. obscura. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047183 obsTM2 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from 10 male thoraxes of D. obscura. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047184 obsTeM1 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from ~20 male testes of D. obscura. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047184 obsTeM2 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from ~20 male testes of D. obscura. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047185 obsWF1 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from 5 female adults of D. obscura. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047185 obsWF2 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from 5 female adults of D. obscura. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047186 obsWM1 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from 5 male adults of D. obscura. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047186 obsWM2 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from 5 male adults of D. obscura. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047187 pseAF1 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from 10 female abdomens of D. pseudoobscura. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy and further confirmed by PCR with Y chromosome specific primers after the extraction of genomic DNA from same individuals. 200 0 Application Read Forward 1 1 Application Read Reverse 101 Illumina HiSeq 2000 PRJDB4576 SAMD00047187 pseAF2 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from 10 female abdomens of D. pseudoobscura. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy and further confirmed by PCR with Y chromosome specific primers after the extraction of genomic DNA from same individuals. 200 0 Application Read Forward 1 1 Application Read Reverse 101 Illumina HiSeq 2000 PRJDB4576 SAMD00047188 pseAM1 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from 10 male abdomens of D. pseudoobscura. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy and further confirmed by PCR with Y chromosome specific primers after the extraction of genomic DNA from same individuals. 200 0 Application Read Forward 1 1 Application Read Reverse 101 Illumina HiSeq 2000 PRJDB4576 SAMD00047188 pseAM2 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from 10 male abdomens of D. pseudoobscura. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy and further confirmed by PCR with Y chromosome specific primers after the extraction of genomic DNA from same individuals. 200 0 Application Read Forward 1 1 Application Read Reverse 101 Illumina HiSeq 2000 PRJDB4576 SAMD00047189 pseAcM1 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from ~20 male accessory glands of D. pseudoobscura. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy and further confirmed by PCR with Y chromosome specific primers after the extraction of genomic DNA from same individuals. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047189 pseAcM2 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from ~20 male accessory glands of D. pseudoobscura. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy and further confirmed by PCR with Y chromosome specific primers after the extraction of genomic DNA from same individuals. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047190 pseAgF1 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from 10 female abdomens of D. pseudoobscura after removing gonads. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy and further confirmed by PCR with Y chromosome specific primers after the extraction of genomic DNA from same individuals. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047190 pseAgF2 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from 10 female abdomens of D. pseudoobscura after removing gonads. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy and further confirmed by PCR with Y chromosome specific primers after the extraction of genomic DNA from same individuals. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047191 pseAgM1 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from 10 male abdomens of D. pseudoobscura after removing gonads. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy and further confirmed by PCR with Y chromosome specific primers after the extraction of genomic DNA from same individuals. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047191 pseAgM2 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from 10 male abdomens of D. pseudoobscura after removing gonads. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy and further confirmed by PCR with Y chromosome specific primers after the extraction of genomic DNA from same individuals. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047192 pseDF1 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from ~20 female imaginal discs (except for genital imaginal disc) of D. pseudoobscura. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy and further confirmed by PCR with Y chromosome specific primers after the extraction of genomic DNA from same individuals. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047192 pseDF2 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from ~20 female imaginal discs (except for genital imaginal disc) of D. pseudoobscura. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy and further confirmed by PCR with Y chromosome specific primers after the extraction of genomic DNA from same individuals. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047193 pseDM1 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from ~20 male imaginal discs (except for genital imaginal disc) of D. pseudoobscura. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy and further confirmed by PCR with Y chromosome specific primers after the extraction of genomic DNA from same individuals. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047193 pseDM2 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from ~20 male imaginal discs (except for genital imaginal disc) of D. pseudoobscura. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy and further confirmed by PCR with Y chromosome specific primers after the extraction of genomic DNA from same individuals. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047194 pseHF1 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from ~20 female heads of D. pseudoobscura. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy and further confirmed by PCR with Y chromosome specific primers after the extraction of genomic DNA from same individuals. 200 0 Application Read Forward 1 1 Application Read Reverse 101 Illumina HiSeq 2000 PRJDB4576 SAMD00047194 pseHF2 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from ~20 female heads of D. pseudoobscura. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy and further confirmed by PCR with Y chromosome specific primers after the extraction of genomic DNA from same individuals. 200 0 Application Read Forward 1 1 Application Read Reverse 101 Illumina HiSeq 2000 PRJDB4576 SAMD00047195 pseHM1 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from ~20 male heads of D. pseudoobscura. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy and further confirmed by PCR with Y chromosome specific primers after the extraction of genomic DNA from same individuals. 200 0 Application Read Forward 1 1 Application Read Reverse 101 Illumina HiSeq 2000 PRJDB4576 SAMD00047195 pseHM2 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from ~20 male heads of D. pseudoobscura. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy and further confirmed by PCR with Y chromosome specific primers after the extraction of genomic DNA from same individuals. 200 0 Application Read Forward 1 1 Application Read Reverse 101 Illumina HiSeq 2000 PRJDB4576 SAMD00047196 pseLF1 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from 5 female 3rd instar larvae of D. pseudoobscura. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy and further confirmed by PCR with Y chromosome specific primers after the extraction of genomic DNA from same individuals. 200 0 Application Read Forward 1 1 Application Read Reverse 101 Illumina HiSeq 2000 PRJDB4576 SAMD00047196 pseLF2 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from 5 female 3rd instar larvae of D. pseudoobscura. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy and further confirmed by PCR with Y chromosome specific primers after the extraction of genomic DNA from same individuals. 200 0 Application Read Forward 1 1 Application Read Reverse 101 Illumina HiSeq 2000 PRJDB4576 SAMD00047197 pseLM1 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from 5 male 3rd instar larvae of D. pseudoobscura. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy and further confirmed by PCR with Y chromosome specific primers after the extraction of genomic DNA from same individuals. 200 0 Application Read Forward 1 1 Application Read Reverse 101 Illumina HiSeq 2000 PRJDB4576 SAMD00047197 pseLM2 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from 5 male 3rd instar larvae of D. pseudoobscura. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy and further confirmed by PCR with Y chromosome specific primers after the extraction of genomic DNA from same individuals. 200 0 Application Read Forward 1 1 Application Read Reverse 101 Illumina HiSeq 2000 PRJDB4576 SAMD00047198 pseLdF1 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from 5 female 3rd instar larvae of D. pseudoobscura after removing imaginal discs but containing genital imaginal disc. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy and further confirmed by PCR with Y chromosome specific primers after the extraction of genomic DNA from same individuals. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047198 pseLdF2 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from 5 female 3rd instar larvae of D. pseudoobscura after removing imaginal discs but containing genital imaginal disc. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy and further confirmed by PCR with Y chromosome specific primers after the extraction of genomic DNA from same individuals. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047199 pseLdM1 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from 5 male 3rd instar larvae of D. pseudoobscura after removing imaginal discs but containing genital imaginal disc. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy and further confirmed by PCR with Y chromosome specific primers after the extraction of genomic DNA from same individuals. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047199 pseLdM2 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from 5 male 3rd instar larvae of D. pseudoobscura after removing imaginal discs but containing genital imaginal disc. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy and further confirmed by PCR with Y chromosome specific primers after the extraction of genomic DNA from same individuals. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047200 pseOF1 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from ~20 female ovaries of D. pseudoobscura. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy and further confirmed by PCR with Y chromosome specific primers after the extraction of genomic DNA from same individuals. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047200 pseOF2 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from ~20 female ovaries of D. pseudoobscura. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy and further confirmed by PCR with Y chromosome specific primers after the extraction of genomic DNA from same individuals. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047201 psePF1 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from 5 female pupae of D. pseudoobscura. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy and further confirmed by PCR with Y chromosome specific primers after the extraction of genomic DNA from same individuals. 200 0 Application Read Forward 1 1 Application Read Reverse 101 Illumina HiSeq 2000 PRJDB4576 SAMD00047201 psePF2 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from 5 female pupae of D. pseudoobscura. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy and further confirmed by PCR with Y chromosome specific primers after the extraction of genomic DNA from same individuals. 200 0 Application Read Forward 1 1 Application Read Reverse 101 Illumina HiSeq 2000 PRJDB4576 SAMD00047202 psePM1 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from 5 male pupae of D. pseudoobscura. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy and further confirmed by PCR with Y chromosome specific primers after the extraction of genomic DNA from same individuals. 200 0 Application Read Forward 1 1 Application Read Reverse 101 Illumina HiSeq 2000 PRJDB4576 SAMD00047202 psePM2 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from 5 male pupae of D. pseudoobscura. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy and further confirmed by PCR with Y chromosome specific primers after the extraction of genomic DNA from same individuals. 200 0 Application Read Forward 1 1 Application Read Reverse 101 Illumina HiSeq 2000 PRJDB4576 SAMD00047203 pseTF1 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from 10 female thoraxes of D. pseudoobscura. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy and further confirmed by PCR with Y chromosome specific primers after the extraction of genomic DNA from same individuals. 200 0 Application Read Forward 1 1 Application Read Reverse 101 Illumina HiSeq 2000 PRJDB4576 SAMD00047203 pseTF2 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from 10 female thoraxes of D. pseudoobscura. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy and further confirmed by PCR with Y chromosome specific primers after the extraction of genomic DNA from same individuals. 200 0 Application Read Forward 1 1 Application Read Reverse 101 Illumina HiSeq 2000 PRJDB4576 SAMD00047204 pseTM1 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from 10 male thoraxes of D. pseudoobscura. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy and further confirmed by PCR with Y chromosome specific primers after the extraction of genomic DNA from same individuals. 200 0 Application Read Forward 1 1 Application Read Reverse 101 Illumina HiSeq 2000 PRJDB4576 SAMD00047204 pseTM2 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from 10 male thoraxes of D. pseudoobscura. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy and further confirmed by PCR with Y chromosome specific primers after the extraction of genomic DNA from same individuals. 200 0 Application Read Forward 1 1 Application Read Reverse 101 Illumina HiSeq 2000 PRJDB4576 SAMD00047205 pseTeM1 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from ~20 male testes of D. pseudoobscura. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy and further confirmed by PCR with Y chromosome specific primers after the extraction of genomic DNA from same individuals. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047205 pseTeM2 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from ~20 male testes of D. pseudoobscura. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy and further confirmed by PCR with Y chromosome specific primers after the extraction of genomic DNA from same individuals. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047206 pseWF1 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from 5 female adults of D. pseudoobscura. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy and further confirmed by PCR with Y chromosome specific primers after the extraction of genomic DNA from same individuals. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047206 pseWF2 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from 5 female adults of D. pseudoobscura. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy and further confirmed by PCR with Y chromosome specific primers after the extraction of genomic DNA from same individuals. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047207 pseWM1 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from 5 male adults of D. pseudoobscura. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy and further confirmed by PCR with Y chromosome specific primers after the extraction of genomic DNA from same individuals. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 PRJDB4576 SAMD00047207 pseWM2 RNA-Seq TRANSCRIPTOMIC RANDOM Total RNA was extracted from 5 male adults of D. pseudoobscura. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy and further confirmed by PCR with Y chromosome specific primers after the extraction of genomic DNA from same individuals. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000