PRJDB5228 SAMD00065457 AMPLICON METAGENOMIC PCR The marine sediment PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooled library was subjected to an Illumina Miseq run with the 2 x 300 cycle sequencing kit (version 3). The remaining forward and reverse sequencing reads were then fused with each other by the program PEAR v0.9.6 (Zhang et al.,2014) with a stringent threshold for merging (p = 0.0001). Among the paired-end reads obtained, we discareded those less than 150 bp in length and those for which 10% or more of nucleotides had low (less than 30) quality values. 600 0 Application Read Forward 1 Illumina MiSeq PRJDB5228 SAMD00065458 AMPLICON METAGENOMIC PCR The marine sediment PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooled library was subjected to an Illumina Miseq run with the 2 x 300 cycle sequencing kit (version 3). The remaining forward and reverse sequencing reads were then fused with each other by the program PEAR v0.9.6 (Zhang et al.,2014) with a stringent threshold for merging (p = 0.0001). Among the paired-end reads obtained, we discareded those less than 150 bp in length and those for which 10% or more of nucleotides had low (less than 30) quality values. 600 0 Application Read Forward 1 Illumina MiSeq PRJDB5228 SAMD00065459 AMPLICON METAGENOMIC PCR The marine sediment PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooled library was subjected to an Illumina Miseq run with the 2 x 300 cycle sequencing kit (version 3). The remaining forward and reverse sequencing reads were then fused with each other by the program PEAR v0.9.6 (Zhang et al.,2014) with a stringent threshold for merging (p = 0.0001). Among the paired-end reads obtained, we discareded those less than 150 bp in length and those for which 10% or more of nucleotides had low (less than 30) quality values. 600 0 Application Read Forward 1 Illumina MiSeq PRJDB5228 SAMD00065460 AMPLICON METAGENOMIC PCR The marine sediment PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooled library was subjected to an Illumina Miseq run with the 2 x 300 cycle sequencing kit (version 3). The remaining forward and reverse sequencing reads were then fused with each other by the program PEAR v0.9.6 (Zhang et al.,2014) with a stringent threshold for merging (p = 0.0001). Among the paired-end reads obtained, we discareded those less than 150 bp in length and those for which 10% or more of nucleotides had low (less than 30) quality values. 600 0 Application Read Forward 1 Illumina MiSeq PRJDB5228 SAMD00065461 AMPLICON METAGENOMIC PCR The marine sediment PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooled library was subjected to an Illumina Miseq run with the 2 x 300 cycle sequencing kit (version 3). The remaining forward and reverse sequencing reads were then fused with each other by the program PEAR v0.9.6 (Zhang et al.,2014) with a stringent threshold for merging (p = 0.0001). Among the paired-end reads obtained, we discareded those less than 150 bp in length and those for which 10% or more of nucleotides had low (less than 30) quality values. 600 0 Application Read Forward 1 Illumina MiSeq PRJDB5228 SAMD00065462 AMPLICON METAGENOMIC PCR The marine sediment PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooled library was subjected to an Illumina Miseq run with the 2 x 300 cycle sequencing kit (version 3). The remaining forward and reverse sequencing reads were then fused with each other by the program PEAR v0.9.6 (Zhang et al.,2014) with a stringent threshold for merging (p = 0.0001). Among the paired-end reads obtained, we discareded those less than 150 bp in length and those for which 10% or more of nucleotides had low (less than 30) quality values. 600 0 Application Read Forward 1 Illumina MiSeq PRJDB5228 SAMD00065463 AMPLICON METAGENOMIC PCR The marine sediment PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooled library was subjected to an Illumina Miseq run with the 2 x 300 cycle sequencing kit (version 3). The remaining forward and reverse sequencing reads were then fused with each other by the program PEAR v0.9.6 (Zhang et al.,2014) with a stringent threshold for merging (p = 0.0001). Among the paired-end reads obtained, we discareded those less than 150 bp in length and those for which 10% or more of nucleotides had low (less than 30) quality values. 600 0 Application Read Forward 1 Illumina MiSeq PRJDB5228 SAMD00065464 AMPLICON METAGENOMIC PCR The marine sediment PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooled library was subjected to an Illumina Miseq run with the 2 x 300 cycle sequencing kit (version 3). The remaining forward and reverse sequencing reads were then fused with each other by the program PEAR v0.9.6 (Zhang et al.,2014) with a stringent threshold for merging (p = 0.0001). Among the paired-end reads obtained, we discareded those less than 150 bp in length and those for which 10% or more of nucleotides had low (less than 30) quality values. 600 0 Application Read Forward 1 Illumina MiSeq PRJDB5228 SAMD00065465 AMPLICON METAGENOMIC PCR The marine sediment PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooled library was subjected to an Illumina Miseq run with the 2 x 300 cycle sequencing kit (version 3). The remaining forward and reverse sequencing reads were then fused with each other by the program PEAR v0.9.6 (Zhang et al.,2014) with a stringent threshold for merging (p = 0.0001). Among the paired-end reads obtained, we discareded those less than 150 bp in length and those for which 10% or more of nucleotides had low (less than 30) quality values. 600 0 Application Read Forward 1 Illumina MiSeq PRJDB5228 SAMD00065466 AMPLICON METAGENOMIC PCR The marine sediment PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooled library was subjected to an Illumina Miseq run with the 2 x 300 cycle sequencing kit (version 3). The remaining forward and reverse sequencing reads were then fused with each other by the program PEAR v0.9.6 (Zhang et al.,2014) with a stringent threshold for merging (p = 0.0001). Among the paired-end reads obtained, we discareded those less than 150 bp in length and those for which 10% or more of nucleotides had low (less than 30) quality values. 600 0 Application Read Forward 1 Illumina MiSeq PRJDB5228 SAMD00065467 AMPLICON METAGENOMIC PCR The marine sediment PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooled library was subjected to an Illumina Miseq run with the 2 x 300 cycle sequencing kit (version 3). The remaining forward and reverse sequencing reads were then fused with each other by the program PEAR v0.9.6 (Zhang et al.,2014) with a stringent threshold for merging (p = 0.0001). Among the paired-end reads obtained, we discareded those less than 150 bp in length and those for which 10% or more of nucleotides had low (less than 30) quality values. 600 0 Application Read Forward 1 Illumina MiSeq PRJDB5228 SAMD00065468 AMPLICON METAGENOMIC PCR The marine sediment PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooled library was subjected to an Illumina Miseq run with the 2 x 300 cycle sequencing kit (version 3). The remaining forward and reverse sequencing reads were then fused with each other by the program PEAR v0.9.6 (Zhang et al.,2014) with a stringent threshold for merging (p = 0.0001). Among the paired-end reads obtained, we discareded those less than 150 bp in length and those for which 10% or more of nucleotides had low (less than 30) quality values. 600 0 Application Read Forward 1 Illumina MiSeq PRJDB5228 SAMD00065469 AMPLICON METAGENOMIC PCR The marine sediment PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooled library was subjected to an Illumina Miseq run with the 2 x 300 cycle sequencing kit (version 3). The remaining forward and reverse sequencing reads were then fused with each other by the program PEAR v0.9.6 (Zhang et al.,2014) with a stringent threshold for merging (p = 0.0001). Among the paired-end reads obtained, we discareded those less than 150 bp in length and those for which 10% or more of nucleotides had low (less than 30) quality values. 600 0 Application Read Forward 1 Illumina MiSeq PRJDB5228 SAMD00065470 AMPLICON METAGENOMIC PCR The marine sediment PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooled library was subjected to an Illumina Miseq run with the 2 x 300 cycle sequencing kit (version 3). The remaining forward and reverse sequencing reads were then fused with each other by the program PEAR v0.9.6 (Zhang et al.,2014) with a stringent threshold for merging (p = 0.0001). Among the paired-end reads obtained, we discareded those less than 150 bp in length and those for which 10% or more of nucleotides had low (less than 30) quality values. 600 0 Application Read Forward 1 Illumina MiSeq PRJDB5228 SAMD00065471 AMPLICON METAGENOMIC PCR The marine sediment PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooled library was subjected to an Illumina Miseq run with the 2 x 300 cycle sequencing kit (version 3). The remaining forward and reverse sequencing reads were then fused with each other by the program PEAR v0.9.6 (Zhang et al.,2014) with a stringent threshold for merging (p = 0.0001). Among the paired-end reads obtained, we discareded those less than 150 bp in length and those for which 10% or more of nucleotides had low (less than 30) quality values. 600 0 Application Read Forward 1 Illumina MiSeq PRJDB5228 SAMD00065472 AMPLICON METAGENOMIC PCR The marine sediment PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooled library was subjected to an Illumina Miseq run with the 2 x 300 cycle sequencing kit (version 3). The remaining forward and reverse sequencing reads were then fused with each other by the program PEAR v0.9.6 (Zhang et al.,2014) with a stringent threshold for merging (p = 0.0001). Among the paired-end reads obtained, we discareded those less than 150 bp in length and those for which 10% or more of nucleotides had low (less than 30) quality values. 600 0 Application Read Forward 1 Illumina MiSeq PRJDB5228 SAMD00065473 AMPLICON METAGENOMIC PCR The marine sediment PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooled library was subjected to an Illumina Miseq run with the 2 x 300 cycle sequencing kit (version 3). The remaining forward and reverse sequencing reads were then fused with each other by the program PEAR v0.9.6 (Zhang et al.,2014) with a stringent threshold for merging (p = 0.0001). Among the paired-end reads obtained, we discareded those less than 150 bp in length and those for which 10% or more of nucleotides had low (less than 30) quality values. 600 0 Application Read Forward 1 Illumina MiSeq PRJDB5228 SAMD00065474 AMPLICON METAGENOMIC PCR The marine sediment PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooled library was subjected to an Illumina Miseq run with the 2 x 300 cycle sequencing kit (version 3). The remaining forward and reverse sequencing reads were then fused with each other by the program PEAR v0.9.6 (Zhang et al.,2014) with a stringent threshold for merging (p = 0.0001). Among the paired-end reads obtained, we discareded those less than 150 bp in length and those for which 10% or more of nucleotides had low (less than 30) quality values. 600 0 Application Read Forward 1 Illumina MiSeq PRJDB5228 SAMD00065475 AMPLICON METAGENOMIC PCR The marine sediment PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooled library was subjected to an Illumina Miseq run with the 2 x 300 cycle sequencing kit (version 3). The remaining forward and reverse sequencing reads were then fused with each other by the program PEAR v0.9.6 (Zhang et al.,2014) with a stringent threshold for merging (p = 0.0001). Among the paired-end reads obtained, we discareded those less than 150 bp in length and those for which 10% or more of nucleotides had low (less than 30) quality values. 600 0 Application Read Forward 1 Illumina MiSeq PRJDB5228 SAMD00065476 AMPLICON METAGENOMIC PCR The marine sediment PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooled library was subjected to an Illumina Miseq run with the 2 x 300 cycle sequencing kit (version 3). The remaining forward and reverse sequencing reads were then fused with each other by the program PEAR v0.9.6 (Zhang et al.,2014) with a stringent threshold for merging (p = 0.0001). Among the paired-end reads obtained, we discareded those less than 150 bp in length and those for which 10% or more of nucleotides had low (less than 30) quality values. 600 0 Application Read Forward 1 Illumina MiSeq PRJDB5228 SAMD00065477 AMPLICON METAGENOMIC PCR The marine sediment PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooled library was subjected to an Illumina Miseq run with the 2 x 300 cycle sequencing kit (version 3). The remaining forward and reverse sequencing reads were then fused with each other by the program PEAR v0.9.6 (Zhang et al.,2014) with a stringent threshold for merging (p = 0.0001). Among the paired-end reads obtained, we discareded those less than 150 bp in length and those for which 10% or more of nucleotides had low (less than 30) quality values. 600 0 Application Read Forward 1 Illumina MiSeq PRJDB5228 SAMD00065478 AMPLICON METAGENOMIC PCR The marine sediment PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooled library was subjected to an Illumina Miseq run with the 2 x 300 cycle sequencing kit (version 3). The remaining forward and reverse sequencing reads were then fused with each other by the program PEAR v0.9.6 (Zhang et al.,2014) with a stringent threshold for merging (p = 0.0001). Among the paired-end reads obtained, we discareded those less than 150 bp in length and those for which 10% or more of nucleotides had low (less than 30) quality values. 600 0 Application Read Forward 1 Illumina MiSeq PRJDB5228 SAMD00065479 AMPLICON METAGENOMIC PCR The marine sediment PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooled library was subjected to an Illumina Miseq run with the 2 x 300 cycle sequencing kit (version 3). The remaining forward and reverse sequencing reads were then fused with each other by the program PEAR v0.9.6 (Zhang et al.,2014) with a stringent threshold for merging (p = 0.0001). Among the paired-end reads obtained, we discareded those less than 150 bp in length and those for which 10% or more of nucleotides had low (less than 30) quality values. 600 0 Application Read Forward 1 Illumina MiSeq PRJDB5228 SAMD00065480 AMPLICON METAGENOMIC PCR The marine sediment PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooled library was subjected to an Illumina Miseq run with the 2 x 300 cycle sequencing kit (version 3). The remaining forward and reverse sequencing reads were then fused with each other by the program PEAR v0.9.6 (Zhang et al.,2014) with a stringent threshold for merging (p = 0.0001). Among the paired-end reads obtained, we discareded those less than 150 bp in length and those for which 10% or more of nucleotides had low (less than 30) quality values. 600 0 Application Read Forward 1 Illumina MiSeq