PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066059 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 460 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066060 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 460 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066061 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 465 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066062 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 465 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066063 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 440 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066064 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 465 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066065 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 465 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066066 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 465 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066067 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 465 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066068 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 446 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066069 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 465 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066070 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 465 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066071 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 465 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066072 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 465 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066073 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 460 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066074 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 465 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066075 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 465 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066076 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 465 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066077 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 465 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066078 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 465 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066079 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 465 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066080 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 466 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066081 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 465 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066082 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 460 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066083 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 465 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066084 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 460 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066085 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 441 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066086 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 460 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066087 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 465 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066088 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 460 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066089 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 441 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066090 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 441 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066091 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 465 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066092 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 465 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066093 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 466 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066094 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 465 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066095 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 465 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066096 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 465 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066097 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 465 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066098 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 440 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066099 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 446 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066100 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 465 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066101 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 465 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066102 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 446 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066103 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 446 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066104 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 446 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066105 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 440 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066106 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 446 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066107 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 463 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066108 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 465 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066109 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 465 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066110 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 460 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066111 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 460 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066112 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 460 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066113 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 465 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066114 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 465 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066115 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 460 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066116 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 465 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066117 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 441 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066118 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 465 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066119 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 465 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066120 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 464 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066121 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 465 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066122 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 465 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066123 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 465 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066124 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 465 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066125 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 465 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066126 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 465 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066127 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 465 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066128 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 446 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066129 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 465 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066130 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 464 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066131 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 465 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066132 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 465 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066133 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 465 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066134 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 465 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066135 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 465 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066136 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 465 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066137 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 460 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066138 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 464 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066139 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 465 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066140 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 465 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066141 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 440 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066142 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 443 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066143 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 465 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066144 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 465 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066145 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 466 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066146 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 465 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066147 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 465 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066148 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 440 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066149 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 465 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066150 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 465 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066151 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 465 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066152 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 465 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066153 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 465 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066154 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 42 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066155 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 440 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066156 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 440 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066157 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 465 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066158 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 445 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066159 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 458 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066160 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 465 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066161 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 465 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066162 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 459 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066163 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 466 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066164 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 440 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066165 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 441 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066166 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 465 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066167 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 465 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066168 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 462 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066169 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 460 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066170 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 465 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066171 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 465 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066172 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 465 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066173 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 440 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066174 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 465 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066175 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 464 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066176 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 465 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066177 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 465 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066178 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 460 0 Application Read Forward 1 Illumina MiSeq PRJDB5278 Each of the 121 Samples was gut metagenome isolated from Litopenaeus Vannamei. Gut bacteria DNA was extracted using the FAST DNA Spin kit (MO BIO Laboratories, Carlsbad, CA, USA) according to the manufacturer???s protocols. SAMD00066179 AMPLICON METAGENOMIC PCR The obtained gut bacteria PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooledlibrary was subjected to an Illumina Miseq run with the 2 x 300 cyclesequencing kit (version 3). Triplicated polymerase chain reaction (PCR) amplicons were pooled together. Polymerase chain reaction amplicons were purified using a PCR fragment purification kit (Takara, Japan) and quantified using a Quant-It Pico Green kit (Invitrogen, USA) with a Qubit fluorometer (Life Technologies, USA). An equimolar amount of PCR amplicons were combined into one pooled sample and submitted to Novogene Beijing for Illumina paired-end (PE) library preparation, cluster generation and 250 bp PE sequencing on an Illumina MiSeq machine. Sequence data were filtered using QIIME (version 1.7.0) (Caporaso et al., 2010) and removed UCHIME using mothur software (version 1.31.2) (Schloss PD et al., 2009; Edgar RC et al., 2011). Raw FASTQ files were de-multiplexed with QIMME v1.7.0 (Caporaso et al., 2010), and the paired reads were joined with FLASH (fast length adjustment of short reads) using default setting (Magoc?? and Salzberg, 2011). The joined pairs were then quality filtered and analysed with QIMME. 465 0 Application Read Forward 1 Illumina MiSeq