PRJDB5320 16S rRNA gene-based sequencing, microbial diversity with chilled foods. The aim of this study was to assess the optimized primer pair for food microbiological divergence with metagenomic analysis. Food samples are complex matrix containing plant DNA, which is developed specific amplification of bacterial DNA in samples rich in plant DNA. We experimentally evaluated primer efficiency by comparing taxonomic distribution of the 16S rRNA amplicons from directly metagenomes. 16S rRNA gene-based sequencing, microbial diversity with chilled foods. bioproject PRJDB5320 PRJDB5320 true The aim of this study was to assess the optimized primer pair for food microbiological divergence with metagenomic analysis. Food samples are complex matrix containing plant DNA, which is developed specific amplification of bacterial DNA in samples rich in plant DNA. We experimentally evaluated primer efficiency by comparing taxonomic distribution of the 16S rRNA amplicons from directly metagenomes.