PRJDB5471 SAMD00071628 S3-Acu-1 AMPLICON METAGENOMIC PCR PCR using barcoded Illumina primers was performed by following the Earth Microbiome protocols (Caporaso et al. 2012), with the following differences: HF Phusion polymerase mix (New England BioLabs, Ipswich, MA) was used and 3% dimethylsulfoxide (DMSO) was added to the reaction mixtures before cycling at 98????????????????????????????????C for 45 s, 50????????????????????????????????C for 60 s, and 72????????????????????????????????C for 90 s. Amplifications for each sample were performed in triplicate and pooled befor normalization based on Quant-iT PicoGreen dsDNA Assay Kit DNA quantification. 500 0 Application Read Forward 1 1 Application Read Reverse 251 Illumina MiSeq PRJDB5471 SAMD00071628 S3-Acu-2 AMPLICON METAGENOMIC PCR PCR using barcoded Illumina primers was performed by following the Earth Microbiome protocols (Caporaso et al. 2012), with the following differences: HF Phusion polymerase mix (New England BioLabs, Ipswich, MA) was used and 3% dimethylsulfoxide (DMSO) was added to the reaction mixtures before cycling at 98????????????????????????????????C for 45 s, 50????????????????????????????????C for 60 s, and 72????????????????????????????????C for 90 s. Amplifications for each sample were performed in triplicate and pooled befor normalization based on Quant-iT PicoGreen dsDNA Assay Kit DNA quantification. 500 0 Application Read Forward 1 1 Application Read Reverse 251 Illumina MiSeq PRJDB5471 SAMD00071628 S3-Acu-3 AMPLICON METAGENOMIC PCR PCR using barcoded Illumina primers was performed by following the Earth Microbiome protocols (Caporaso et al. 2012), with the following differences: HF Phusion polymerase mix (New England BioLabs, Ipswich, MA) was used and 3% dimethylsulfoxide (DMSO) was added to the reaction mixtures before cycling at 98????????????????????????????????C for 45 s, 50????????????????????????????????C for 60 s, and 72????????????????????????????????C for 90 s. Amplifications for each sample were performed in triplicate and pooled befor normalization based on Quant-iT PicoGreen dsDNA Assay Kit DNA quantification. 500 0 Application Read Forward 1 1 Application Read Reverse 251 Illumina MiSeq PRJDB5471 SAMD00071628 S3-Acu-4 AMPLICON METAGENOMIC PCR PCR using barcoded Illumina primers was performed by following the Earth Microbiome protocols (Caporaso et al. 2012), with the following differences: HF Phusion polymerase mix (New England BioLabs, Ipswich, MA) was used and 3% dimethylsulfoxide (DMSO) was added to the reaction mixtures before cycling at 98????????????????????????????????C for 45 s, 50????????????????????????????????C for 60 s, and 72????????????????????????????????C for 90 s. Amplifications for each sample were performed in triplicate and pooled befor normalization based on Quant-iT PicoGreen dsDNA Assay Kit DNA quantification. 500 0 Application Read Forward 1 1 Application Read Reverse 251 Illumina MiSeq PRJDB5471 SAMD00071628 S3-Acu-5 AMPLICON METAGENOMIC PCR PCR using barcoded Illumina primers was performed by following the Earth Microbiome protocols (Caporaso et al. 2012), with the following differences: HF Phusion polymerase mix (New England BioLabs, Ipswich, MA) was used and 3% dimethylsulfoxide (DMSO) was added to the reaction mixtures before cycling at 98????????????????????????????????C for 45 s, 50????????????????????????????????C for 60 s, and 72????????????????????????????????C for 90 s. Amplifications for each sample were performed in triplicate and pooled befor normalization based on Quant-iT PicoGreen dsDNA Assay Kit DNA quantification. 500 0 Application Read Forward 1 1 Application Read Reverse 251 Illumina MiSeq PRJDB5471 SAMD00071629 S3-Sub-1 AMPLICON METAGENOMIC PCR PCR using barcoded Illumina primers was performed by following the Earth Microbiome protocols (Caporaso et al. 2012), with the following differences: HF Phusion polymerase mix (New England BioLabs, Ipswich, MA) was used and 3% dimethylsulfoxide (DMSO) was added to the reaction mixtures before cycling at 98????????????????????????????????C for 45 s, 50????????????????????????????????C for 60 s, and 72????????????????????????????????C for 90 s. Amplifications for each sample were performed in triplicate and pooled befor normalization based on Quant-iT PicoGreen dsDNA Assay Kit DNA quantification. 500 0 Application Read Forward 1 1 Application Read Reverse 251 Illumina MiSeq PRJDB5471 SAMD00071629 S3-Sub-2 AMPLICON METAGENOMIC PCR PCR using barcoded Illumina primers was performed by following the Earth Microbiome protocols (Caporaso et al. 2012), with the following differences: HF Phusion polymerase mix (New England BioLabs, Ipswich, MA) was used and 3% dimethylsulfoxide (DMSO) was added to the reaction mixtures before cycling at 98????????????????????????????????C for 45 s, 50????????????????????????????????C for 60 s, and 72????????????????????????????????C for 90 s. Amplifications for each sample were performed in triplicate and pooled befor normalization based on Quant-iT PicoGreen dsDNA Assay Kit DNA quantification. 500 0 Application Read Forward 1 1 Application Read Reverse 251 Illumina MiSeq PRJDB5471 SAMD00071629 S3-Sub-3 AMPLICON METAGENOMIC PCR PCR using barcoded Illumina primers was performed by following the Earth Microbiome protocols (Caporaso et al. 2012), with the following differences: HF Phusion polymerase mix (New England BioLabs, Ipswich, MA) was used and 3% dimethylsulfoxide (DMSO) was added to the reaction mixtures before cycling at 98????????????????????????????????C for 45 s, 50????????????????????????????????C for 60 s, and 72????????????????????????????????C for 90 s. Amplifications for each sample were performed in triplicate and pooled befor normalization based on Quant-iT PicoGreen dsDNA Assay Kit DNA quantification. 500 0 Application Read Forward 1 1 Application Read Reverse 251 Illumina MiSeq PRJDB5471 SAMD00071629 S3-Sub-4 AMPLICON METAGENOMIC PCR PCR using barcoded Illumina primers was performed by following the Earth Microbiome protocols (Caporaso et al. 2012), with the following differences: HF Phusion polymerase mix (New England BioLabs, Ipswich, MA) was used and 3% dimethylsulfoxide (DMSO) was added to the reaction mixtures before cycling at 98????????????????????????????????C for 45 s, 50????????????????????????????????C for 60 s, and 72????????????????????????????????C for 90 s. Amplifications for each sample were performed in triplicate and pooled befor normalization based on Quant-iT PicoGreen dsDNA Assay Kit DNA quantification. 500 0 Application Read Forward 1 1 Application Read Reverse 251 Illumina MiSeq PRJDB5471 SAMD00071629 S3-Sub-5 AMPLICON METAGENOMIC PCR PCR using barcoded Illumina primers was performed by following the Earth Microbiome protocols (Caporaso et al. 2012), with the following differences: HF Phusion polymerase mix (New England BioLabs, Ipswich, MA) was used and 3% dimethylsulfoxide (DMSO) was added to the reaction mixtures before cycling at 98????????????????????????????????C for 45 s, 50????????????????????????????????C for 60 s, and 72????????????????????????????????C for 90 s. Amplifications for each sample were performed in triplicate and pooled befor normalization based on Quant-iT PicoGreen dsDNA Assay Kit DNA quantification. 500 0 Application Read Forward 1 1 Application Read Reverse 251 Illumina MiSeq PRJDB5471 SAMD00071624 S1-Acu-1 AMPLICON METAGENOMIC PCR PCR using barcoded Illumina primers was performed by following the Earth Microbiome protocols (Caporaso et al. 2012), with the following differences: HF Phusion polymerase mix (New England BioLabs, Ipswich, MA) was used and 3% dimethylsulfoxide (DMSO) was added to the reaction mixtures before cycling at 98????????????????????????????????C for 45 s, 50????????????????????????????????C for 60 s, and 72????????????????????????????????C for 90 s. Amplifications for each sample were performed in triplicate and pooled befor normalization based on Quant-iT PicoGreen dsDNA Assay Kit DNA quantification. 500 0 Application Read Forward 1 1 Application Read Reverse 251 Illumina MiSeq PRJDB5471 SAMD00071624 S1-Acu-2 AMPLICON METAGENOMIC PCR PCR using barcoded Illumina primers was performed by following the Earth Microbiome protocols (Caporaso et al. 2012), with the following differences: HF Phusion polymerase mix (New England BioLabs, Ipswich, MA) was used and 3% dimethylsulfoxide (DMSO) was added to the reaction mixtures before cycling at 98????????????????????????????????C for 45 s, 50????????????????????????????????C for 60 s, and 72????????????????????????????????C for 90 s. Amplifications for each sample were performed in triplicate and pooled befor normalization based on Quant-iT PicoGreen dsDNA Assay Kit DNA quantification. 500 0 Application Read Forward 1 1 Application Read Reverse 251 Illumina MiSeq PRJDB5471 SAMD00071624 S1-Acu-3 AMPLICON METAGENOMIC PCR PCR using barcoded Illumina primers was performed by following the Earth Microbiome protocols (Caporaso et al. 2012), with the following differences: HF Phusion polymerase mix (New England BioLabs, Ipswich, MA) was used and 3% dimethylsulfoxide (DMSO) was added to the reaction mixtures before cycling at 98????????????????????????????????C for 45 s, 50????????????????????????????????C for 60 s, and 72????????????????????????????????C for 90 s. Amplifications for each sample were performed in triplicate and pooled befor normalization based on Quant-iT PicoGreen dsDNA Assay Kit DNA quantification. 500 0 Application Read Forward 1 1 Application Read Reverse 251 Illumina MiSeq PRJDB5471 SAMD00071624 S1-Acu-4 AMPLICON METAGENOMIC PCR PCR using barcoded Illumina primers was performed by following the Earth Microbiome protocols (Caporaso et al. 2012), with the following differences: HF Phusion polymerase mix (New England BioLabs, Ipswich, MA) was used and 3% dimethylsulfoxide (DMSO) was added to the reaction mixtures before cycling at 98????????????????????????????????C for 45 s, 50????????????????????????????????C for 60 s, and 72????????????????????????????????C for 90 s. Amplifications for each sample were performed in triplicate and pooled befor normalization based on Quant-iT PicoGreen dsDNA Assay Kit DNA quantification. 500 0 Application Read Forward 1 1 Application Read Reverse 251 Illumina MiSeq PRJDB5471 SAMD00071624 S1-Acu-5 AMPLICON METAGENOMIC PCR PCR using barcoded Illumina primers was performed by following the Earth Microbiome protocols (Caporaso et al. 2012), with the following differences: HF Phusion polymerase mix (New England BioLabs, Ipswich, MA) was used and 3% dimethylsulfoxide (DMSO) was added to the reaction mixtures before cycling at 98????????????????????????????????C for 45 s, 50????????????????????????????????C for 60 s, and 72????????????????????????????????C for 90 s. Amplifications for each sample were performed in triplicate and pooled befor normalization based on Quant-iT PicoGreen dsDNA Assay Kit DNA quantification. 500 0 Application Read Forward 1 1 Application Read Reverse 251 Illumina MiSeq PRJDB5471 SAMD00071624 S1-Acu-6 AMPLICON METAGENOMIC PCR PCR using barcoded Illumina primers was performed by following the Earth Microbiome protocols (Caporaso et al. 2012), with the following differences: HF Phusion polymerase mix (New England BioLabs, Ipswich, MA) was used and 3% dimethylsulfoxide (DMSO) was added to the reaction mixtures before cycling at 98????????????????????????????????C for 45 s, 50????????????????????????????????C for 60 s, and 72????????????????????????????????C for 90 s. Amplifications for each sample were performed in triplicate and pooled befor normalization based on Quant-iT PicoGreen dsDNA Assay Kit DNA quantification. 500 0 Application Read Forward 1 1 Application Read Reverse 251 Illumina MiSeq PRJDB5471 SAMD00071625 S1-Sub-1 AMPLICON METAGENOMIC PCR PCR using barcoded Illumina primers was performed by following the Earth Microbiome protocols (Caporaso et al. 2012), with the following differences: HF Phusion polymerase mix (New England BioLabs, Ipswich, MA) was used and 3% dimethylsulfoxide (DMSO) was added to the reaction mixtures before cycling at 98????????????????????????????????C for 45 s, 50????????????????????????????????C for 60 s, and 72????????????????????????????????C for 90 s. Amplifications for each sample were performed in triplicate and pooled befor normalization based on Quant-iT PicoGreen dsDNA Assay Kit DNA quantification. 500 0 Application Read Forward 1 1 Application Read Reverse 251 Illumina MiSeq PRJDB5471 SAMD00071625 S1-Sub-2 AMPLICON METAGENOMIC PCR PCR using barcoded Illumina primers was performed by following the Earth Microbiome protocols (Caporaso et al. 2012), with the following differences: HF Phusion polymerase mix (New England BioLabs, Ipswich, MA) was used and 3% dimethylsulfoxide (DMSO) was added to the reaction mixtures before cycling at 98????????????????????????????????C for 45 s, 50????????????????????????????????C for 60 s, and 72????????????????????????????????C for 90 s. Amplifications for each sample were performed in triplicate and pooled befor normalization based on Quant-iT PicoGreen dsDNA Assay Kit DNA quantification. 500 0 Application Read Forward 1 1 Application Read Reverse 251 Illumina MiSeq PRJDB5471 SAMD00071625 S1-Sub-3 AMPLICON METAGENOMIC PCR PCR using barcoded Illumina primers was performed by following the Earth Microbiome protocols (Caporaso et al. 2012), with the following differences: HF Phusion polymerase mix (New England BioLabs, Ipswich, MA) was used and 3% dimethylsulfoxide (DMSO) was added to the reaction mixtures before cycling at 98????????????????????????????????C for 45 s, 50????????????????????????????????C for 60 s, and 72????????????????????????????????C for 90 s. Amplifications for each sample were performed in triplicate and pooled befor normalization based on Quant-iT PicoGreen dsDNA Assay Kit DNA quantification. 500 0 Application Read Forward 1 1 Application Read Reverse 251 Illumina MiSeq PRJDB5471 SAMD00071625 S1-Sub-4 AMPLICON METAGENOMIC PCR PCR using barcoded Illumina primers was performed by following the Earth Microbiome protocols (Caporaso et al. 2012), with the following differences: HF Phusion polymerase mix (New England BioLabs, Ipswich, MA) was used and 3% dimethylsulfoxide (DMSO) was added to the reaction mixtures before cycling at 98????????????????????????????????C for 45 s, 50????????????????????????????????C for 60 s, and 72????????????????????????????????C for 90 s. Amplifications for each sample were performed in triplicate and pooled befor normalization based on Quant-iT PicoGreen dsDNA Assay Kit DNA quantification. 500 0 Application Read Forward 1 1 Application Read Reverse 251 Illumina MiSeq PRJDB5471 SAMD00071625 S1-Sub-5 AMPLICON METAGENOMIC PCR PCR using barcoded Illumina primers was performed by following the Earth Microbiome protocols (Caporaso et al. 2012), with the following differences: HF Phusion polymerase mix (New England BioLabs, Ipswich, MA) was used and 3% dimethylsulfoxide (DMSO) was added to the reaction mixtures before cycling at 98????????????????????????????????C for 45 s, 50????????????????????????????????C for 60 s, and 72????????????????????????????????C for 90 s. Amplifications for each sample were performed in triplicate and pooled befor normalization based on Quant-iT PicoGreen dsDNA Assay Kit DNA quantification. 500 0 Application Read Forward 1 1 Application Read Reverse 251 Illumina MiSeq PRJDB5471 SAMD00071626 S2-Acu-1 AMPLICON METAGENOMIC PCR PCR using barcoded Illumina primers was performed by following the Earth Microbiome protocols (Caporaso et al. 2012), with the following differences: HF Phusion polymerase mix (New England BioLabs, Ipswich, MA) was used and 3% dimethylsulfoxide (DMSO) was added to the reaction mixtures before cycling at 98????????????????????????????????C for 45 s, 50????????????????????????????????C for 60 s, and 72????????????????????????????????C for 90 s. Amplifications for each sample were performed in triplicate and pooled befor normalization based on Quant-iT PicoGreen dsDNA Assay Kit DNA quantification. 500 0 Application Read Forward 1 1 Application Read Reverse 251 Illumina MiSeq PRJDB5471 SAMD00071626 S2-Acu-2 AMPLICON METAGENOMIC PCR PCR using barcoded Illumina primers was performed by following the Earth Microbiome protocols (Caporaso et al. 2012), with the following differences: HF Phusion polymerase mix (New England BioLabs, Ipswich, MA) was used and 3% dimethylsulfoxide (DMSO) was added to the reaction mixtures before cycling at 98????????????????????????????????C for 45 s, 50????????????????????????????????C for 60 s, and 72????????????????????????????????C for 90 s. Amplifications for each sample were performed in triplicate and pooled befor normalization based on Quant-iT PicoGreen dsDNA Assay Kit DNA quantification. 500 0 Application Read Forward 1 1 Application Read Reverse 251 Illumina MiSeq PRJDB5471 SAMD00071626 S2-Acu-3 AMPLICON METAGENOMIC PCR PCR using barcoded Illumina primers was performed by following the Earth Microbiome protocols (Caporaso et al. 2012), with the following differences: HF Phusion polymerase mix (New England BioLabs, Ipswich, MA) was used and 3% dimethylsulfoxide (DMSO) was added to the reaction mixtures before cycling at 98????????????????????????????????C for 45 s, 50????????????????????????????????C for 60 s, and 72????????????????????????????????C for 90 s. Amplifications for each sample were performed in triplicate and pooled befor normalization based on Quant-iT PicoGreen dsDNA Assay Kit DNA quantification. 500 0 Application Read Forward 1 1 Application Read Reverse 251 Illumina MiSeq PRJDB5471 SAMD00071626 S2-Acu-4 AMPLICON METAGENOMIC PCR PCR using barcoded Illumina primers was performed by following the Earth Microbiome protocols (Caporaso et al. 2012), with the following differences: HF Phusion polymerase mix (New England BioLabs, Ipswich, MA) was used and 3% dimethylsulfoxide (DMSO) was added to the reaction mixtures before cycling at 98????????????????????????????????C for 45 s, 50????????????????????????????????C for 60 s, and 72????????????????????????????????C for 90 s. Amplifications for each sample were performed in triplicate and pooled befor normalization based on Quant-iT PicoGreen dsDNA Assay Kit DNA quantification. 500 0 Application Read Forward 1 1 Application Read Reverse 251 Illumina MiSeq PRJDB5471 SAMD00071626 S2-Acu-5 AMPLICON METAGENOMIC PCR PCR using barcoded Illumina primers was performed by following the Earth Microbiome protocols (Caporaso et al. 2012), with the following differences: HF Phusion polymerase mix (New England BioLabs, Ipswich, MA) was used and 3% dimethylsulfoxide (DMSO) was added to the reaction mixtures before cycling at 98????????????????????????????????C for 45 s, 50????????????????????????????????C for 60 s, and 72????????????????????????????????C for 90 s. Amplifications for each sample were performed in triplicate and pooled befor normalization based on Quant-iT PicoGreen dsDNA Assay Kit DNA quantification. 500 0 Application Read Forward 1 1 Application Read Reverse 251 Illumina MiSeq PRJDB5471 SAMD00071626 S2-Acu-6 AMPLICON METAGENOMIC PCR PCR using barcoded Illumina primers was performed by following the Earth Microbiome protocols (Caporaso et al. 2012), with the following differences: HF Phusion polymerase mix (New England BioLabs, Ipswich, MA) was used and 3% dimethylsulfoxide (DMSO) was added to the reaction mixtures before cycling at 98????????????????????????????????C for 45 s, 50????????????????????????????????C for 60 s, and 72????????????????????????????????C for 90 s. Amplifications for each sample were performed in triplicate and pooled befor normalization based on Quant-iT PicoGreen dsDNA Assay Kit DNA quantification. 500 0 Application Read Forward 1 1 Application Read Reverse 251 Illumina MiSeq PRJDB5471 SAMD00071627 S2-Sub-1 AMPLICON METAGENOMIC PCR PCR using barcoded Illumina primers was performed by following the Earth Microbiome protocols (Caporaso et al. 2012), with the following differences: HF Phusion polymerase mix (New England BioLabs, Ipswich, MA) was used and 3% dimethylsulfoxide (DMSO) was added to the reaction mixtures before cycling at 98????????????????????????????????C for 45 s, 50????????????????????????????????C for 60 s, and 72????????????????????????????????C for 90 s. Amplifications for each sample were performed in triplicate and pooled befor normalization based on Quant-iT PicoGreen dsDNA Assay Kit DNA quantification. 500 0 Application Read Forward 1 1 Application Read Reverse 251 Illumina MiSeq PRJDB5471 SAMD00071627 S2-Sub-2 AMPLICON METAGENOMIC PCR PCR using barcoded Illumina primers was performed by following the Earth Microbiome protocols (Caporaso et al. 2012), with the following differences: HF Phusion polymerase mix (New England BioLabs, Ipswich, MA) was used and 3% dimethylsulfoxide (DMSO) was added to the reaction mixtures before cycling at 98????????????????????????????????C for 45 s, 50????????????????????????????????C for 60 s, and 72????????????????????????????????C for 90 s. Amplifications for each sample were performed in triplicate and pooled befor normalization based on Quant-iT PicoGreen dsDNA Assay Kit DNA quantification. 500 0 Application Read Forward 1 1 Application Read Reverse 251 Illumina MiSeq PRJDB5471 SAMD00071627 S2-Sub-3 AMPLICON METAGENOMIC PCR PCR using barcoded Illumina primers was performed by following the Earth Microbiome protocols (Caporaso et al. 2012), with the following differences: HF Phusion polymerase mix (New England BioLabs, Ipswich, MA) was used and 3% dimethylsulfoxide (DMSO) was added to the reaction mixtures before cycling at 98????????????????????????????????C for 45 s, 50????????????????????????????????C for 60 s, and 72????????????????????????????????C for 90 s. Amplifications for each sample were performed in triplicate and pooled befor normalization based on Quant-iT PicoGreen dsDNA Assay Kit DNA quantification. 500 0 Application Read Forward 1 1 Application Read Reverse 251 Illumina MiSeq PRJDB5471 SAMD00071627 S2-Sub-4 AMPLICON METAGENOMIC PCR PCR using barcoded Illumina primers was performed by following the Earth Microbiome protocols (Caporaso et al. 2012), with the following differences: HF Phusion polymerase mix (New England BioLabs, Ipswich, MA) was used and 3% dimethylsulfoxide (DMSO) was added to the reaction mixtures before cycling at 98????????????????????????????????C for 45 s, 50????????????????????????????????C for 60 s, and 72????????????????????????????????C for 90 s. Amplifications for each sample were performed in triplicate and pooled befor normalization based on Quant-iT PicoGreen dsDNA Assay Kit DNA quantification. 500 0 Application Read Forward 1 1 Application Read Reverse 251 Illumina MiSeq PRJDB5471 SAMD00071627 S2-Sub-5 AMPLICON METAGENOMIC PCR PCR using barcoded Illumina primers was performed by following the Earth Microbiome protocols (Caporaso et al. 2012), with the following differences: HF Phusion polymerase mix (New England BioLabs, Ipswich, MA) was used and 3% dimethylsulfoxide (DMSO) was added to the reaction mixtures before cycling at 98????????????????????????????????C for 45 s, 50????????????????????????????????C for 60 s, and 72????????????????????????????????C for 90 s. Amplifications for each sample were performed in triplicate and pooled befor normalization based on Quant-iT PicoGreen dsDNA Assay Kit DNA quantification. 500 0 Application Read Forward 1 1 Application Read Reverse 251 Illumina MiSeq PRJDB5471 SAMD00071630 S4-Ram-1 AMPLICON METAGENOMIC PCR PCR using barcoded Illumina primers was performed by following the Earth Microbiome protocols (Caporaso et al. 2012), with the following differences: HF Phusion polymerase mix (New England BioLabs, Ipswich, MA) was used and 3% dimethylsulfoxide (DMSO) was added to the reaction mixtures before cycling at 98????????????????????????????????C for 45 s, 50????????????????????????????????C for 60 s, and 72????????????????????????????????C for 90 s. Amplifications for each sample were performed in triplicate and pooled befor normalization based on Quant-iT PicoGreen dsDNA Assay Kit DNA quantification. 500 0 Application Read Forward 1 1 Application Read Reverse 251 Illumina MiSeq PRJDB5471 SAMD00071630 S4-Ram-2 AMPLICON METAGENOMIC PCR PCR using barcoded Illumina primers was performed by following the Earth Microbiome protocols (Caporaso et al. 2012), with the following differences: HF Phusion polymerase mix (New England BioLabs, Ipswich, MA) was used and 3% dimethylsulfoxide (DMSO) was added to the reaction mixtures before cycling at 98????????????????????????????????C for 45 s, 50????????????????????????????????C for 60 s, and 72????????????????????????????????C for 90 s. Amplifications for each sample were performed in triplicate and pooled befor normalization based on Quant-iT PicoGreen dsDNA Assay Kit DNA quantification. 500 0 Application Read Forward 1 1 Application Read Reverse 251 Illumina MiSeq PRJDB5471 SAMD00071630 S4-Ram-3 AMPLICON METAGENOMIC PCR PCR using barcoded Illumina primers was performed by following the Earth Microbiome protocols (Caporaso et al. 2012), with the following differences: HF Phusion polymerase mix (New England BioLabs, Ipswich, MA) was used and 3% dimethylsulfoxide (DMSO) was added to the reaction mixtures before cycling at 98????????????????????????????????C for 45 s, 50????????????????????????????????C for 60 s, and 72????????????????????????????????C for 90 s. Amplifications for each sample were performed in triplicate and pooled befor normalization based on Quant-iT PicoGreen dsDNA Assay Kit DNA quantification. 500 0 Application Read Forward 1 1 Application Read Reverse 251 Illumina MiSeq PRJDB5471 SAMD00071630 S4-Ram-4 AMPLICON METAGENOMIC PCR PCR using barcoded Illumina primers was performed by following the Earth Microbiome protocols (Caporaso et al. 2012), with the following differences: HF Phusion polymerase mix (New England BioLabs, Ipswich, MA) was used and 3% dimethylsulfoxide (DMSO) was added to the reaction mixtures before cycling at 98????????????????????????????????C for 45 s, 50????????????????????????????????C for 60 s, and 72????????????????????????????????C for 90 s. Amplifications for each sample were performed in triplicate and pooled befor normalization based on Quant-iT PicoGreen dsDNA Assay Kit DNA quantification. 500 0 Application Read Forward 1 1 Application Read Reverse 251 Illumina MiSeq PRJDB5471 SAMD00071630 S4-Ram-5 AMPLICON METAGENOMIC PCR PCR using barcoded Illumina primers was performed by following the Earth Microbiome protocols (Caporaso et al. 2012), with the following differences: HF Phusion polymerase mix (New England BioLabs, Ipswich, MA) was used and 3% dimethylsulfoxide (DMSO) was added to the reaction mixtures before cycling at 98????????????????????????????????C for 45 s, 50????????????????????????????????C for 60 s, and 72????????????????????????????????C for 90 s. Amplifications for each sample were performed in triplicate and pooled befor normalization based on Quant-iT PicoGreen dsDNA Assay Kit DNA quantification. 500 0 Application Read Forward 1 1 Application Read Reverse 251 Illumina MiSeq PRJDB5471 SAMD00071632 S4-Sub-1 AMPLICON METAGENOMIC PCR PCR using barcoded Illumina primers was performed by following the Earth Microbiome protocols (Caporaso et al. 2012), with the following differences: HF Phusion polymerase mix (New England BioLabs, Ipswich, MA) was used and 3% dimethylsulfoxide (DMSO) was added to the reaction mixtures before cycling at 98????????????????????????????????C for 45 s, 50????????????????????????????????C for 60 s, and 72????????????????????????????????C for 90 s. Amplifications for each sample were performed in triplicate and pooled befor normalization based on Quant-iT PicoGreen dsDNA Assay Kit DNA quantification. 500 0 Application Read Forward 1 1 Application Read Reverse 251 Illumina MiSeq PRJDB5471 SAMD00071632 S4-Sub-2 AMPLICON METAGENOMIC PCR PCR using barcoded Illumina primers was performed by following the Earth Microbiome protocols (Caporaso et al. 2012), with the following differences: HF Phusion polymerase mix (New England BioLabs, Ipswich, MA) was used and 3% dimethylsulfoxide (DMSO) was added to the reaction mixtures before cycling at 98????????????????????????????????C for 45 s, 50????????????????????????????????C for 60 s, and 72????????????????????????????????C for 90 s. Amplifications for each sample were performed in triplicate and pooled befor normalization based on Quant-iT PicoGreen dsDNA Assay Kit DNA quantification. 500 0 Application Read Forward 1 1 Application Read Reverse 251 Illumina MiSeq PRJDB5471 SAMD00071632 S4-Sub-3 AMPLICON METAGENOMIC PCR PCR using barcoded Illumina primers was performed by following the Earth Microbiome protocols (Caporaso et al. 2012), with the following differences: HF Phusion polymerase mix (New England BioLabs, Ipswich, MA) was used and 3% dimethylsulfoxide (DMSO) was added to the reaction mixtures before cycling at 98????????????????????????????????C for 45 s, 50????????????????????????????????C for 60 s, and 72????????????????????????????????C for 90 s. Amplifications for each sample were performed in triplicate and pooled befor normalization based on Quant-iT PicoGreen dsDNA Assay Kit DNA quantification. 500 0 Application Read Forward 1 1 Application Read Reverse 251 Illumina MiSeq PRJDB5471 SAMD00071632 S4-Sub-4 AMPLICON METAGENOMIC PCR PCR using barcoded Illumina primers was performed by following the Earth Microbiome protocols (Caporaso et al. 2012), with the following differences: HF Phusion polymerase mix (New England BioLabs, Ipswich, MA) was used and 3% dimethylsulfoxide (DMSO) was added to the reaction mixtures before cycling at 98????????????????????????????????C for 45 s, 50????????????????????????????????C for 60 s, and 72????????????????????????????????C for 90 s. Amplifications for each sample were performed in triplicate and pooled befor normalization based on Quant-iT PicoGreen dsDNA Assay Kit DNA quantification. 500 0 Application Read Forward 1 1 Application Read Reverse 251 Illumina MiSeq PRJDB5471 SAMD00071632 S4-Sub-5 AMPLICON METAGENOMIC PCR PCR using barcoded Illumina primers was performed by following the Earth Microbiome protocols (Caporaso et al. 2012), with the following differences: HF Phusion polymerase mix (New England BioLabs, Ipswich, MA) was used and 3% dimethylsulfoxide (DMSO) was added to the reaction mixtures before cycling at 98????????????????????????????????C for 45 s, 50????????????????????????????????C for 60 s, and 72????????????????????????????????C for 90 s. Amplifications for each sample were performed in triplicate and pooled befor normalization based on Quant-iT PicoGreen dsDNA Assay Kit DNA quantification. 500 0 Application Read Forward 1 1 Application Read Reverse 251 Illumina MiSeq PRJDB5471 SAMD00071631 S5-Ram-1 AMPLICON METAGENOMIC PCR PCR using barcoded Illumina primers was performed by following the Earth Microbiome protocols (Caporaso et al. 2012), with the following differences: HF Phusion polymerase mix (New England BioLabs, Ipswich, MA) was used and 3% dimethylsulfoxide (DMSO) was added to the reaction mixtures before cycling at 98????????????????????????????????C for 45 s, 50????????????????????????????????C for 60 s, and 72????????????????????????????????C for 90 s. Amplifications for each sample were performed in triplicate and pooled befor normalization based on Quant-iT PicoGreen dsDNA Assay Kit DNA quantification. 500 0 Application Read Forward 1 1 Application Read Reverse 251 Illumina MiSeq PRJDB5471 SAMD00071631 S5-Ram-2 AMPLICON METAGENOMIC PCR PCR using barcoded Illumina primers was performed by following the Earth Microbiome protocols (Caporaso et al. 2012), with the following differences: HF Phusion polymerase mix (New England BioLabs, Ipswich, MA) was used and 3% dimethylsulfoxide (DMSO) was added to the reaction mixtures before cycling at 98????????????????????????????????C for 45 s, 50????????????????????????????????C for 60 s, and 72????????????????????????????????C for 90 s. Amplifications for each sample were performed in triplicate and pooled befor normalization based on Quant-iT PicoGreen dsDNA Assay Kit DNA quantification. 500 0 Application Read Forward 1 1 Application Read Reverse 251 Illumina MiSeq PRJDB5471 SAMD00071631 S5-Ram-3 AMPLICON METAGENOMIC PCR PCR using barcoded Illumina primers was performed by following the Earth Microbiome protocols (Caporaso et al. 2012), with the following differences: HF Phusion polymerase mix (New England BioLabs, Ipswich, MA) was used and 3% dimethylsulfoxide (DMSO) was added to the reaction mixtures before cycling at 98????????????????????????????????C for 45 s, 50????????????????????????????????C for 60 s, and 72????????????????????????????????C for 90 s. Amplifications for each sample were performed in triplicate and pooled befor normalization based on Quant-iT PicoGreen dsDNA Assay Kit DNA quantification. 500 0 Application Read Forward 1 1 Application Read Reverse 251 Illumina MiSeq PRJDB5471 SAMD00071631 S5-Ram-4 AMPLICON METAGENOMIC PCR PCR using barcoded Illumina primers was performed by following the Earth Microbiome protocols (Caporaso et al. 2012), with the following differences: HF Phusion polymerase mix (New England BioLabs, Ipswich, MA) was used and 3% dimethylsulfoxide (DMSO) was added to the reaction mixtures before cycling at 98????????????????????????????????C for 45 s, 50????????????????????????????????C for 60 s, and 72????????????????????????????????C for 90 s. Amplifications for each sample were performed in triplicate and pooled befor normalization based on Quant-iT PicoGreen dsDNA Assay Kit DNA quantification. 500 0 Application Read Forward 1 1 Application Read Reverse 251 Illumina MiSeq PRJDB5471 SAMD00071631 S5-Ram-5 AMPLICON METAGENOMIC PCR PCR using barcoded Illumina primers was performed by following the Earth Microbiome protocols (Caporaso et al. 2012), with the following differences: HF Phusion polymerase mix (New England BioLabs, Ipswich, MA) was used and 3% dimethylsulfoxide (DMSO) was added to the reaction mixtures before cycling at 98????????????????????????????????C for 45 s, 50????????????????????????????????C for 60 s, and 72????????????????????????????????C for 90 s. Amplifications for each sample were performed in triplicate and pooled befor normalization based on Quant-iT PicoGreen dsDNA Assay Kit DNA quantification. 500 0 Application Read Forward 1 1 Application Read Reverse 251 Illumina MiSeq PRJDB5471 SAMD00071633 S5-Sub-1 AMPLICON METAGENOMIC PCR PCR using barcoded Illumina primers was performed by following the Earth Microbiome protocols (Caporaso et al. 2012), with the following differences: HF Phusion polymerase mix (New England BioLabs, Ipswich, MA) was used and 3% dimethylsulfoxide (DMSO) was added to the reaction mixtures before cycling at 98????????????????????????????????C for 45 s, 50????????????????????????????????C for 60 s, and 72????????????????????????????????C for 90 s. Amplifications for each sample were performed in triplicate and pooled befor normalization based on Quant-iT PicoGreen dsDNA Assay Kit DNA quantification. 500 0 Application Read Forward 1 1 Application Read Reverse 251 Illumina MiSeq PRJDB5471 SAMD00071633 S5-Sub-2 AMPLICON METAGENOMIC PCR PCR using barcoded Illumina primers was performed by following the Earth Microbiome protocols (Caporaso et al. 2012), with the following differences: HF Phusion polymerase mix (New England BioLabs, Ipswich, MA) was used and 3% dimethylsulfoxide (DMSO) was added to the reaction mixtures before cycling at 98????????????????????????????????C for 45 s, 50????????????????????????????????C for 60 s, and 72????????????????????????????????C for 90 s. Amplifications for each sample were performed in triplicate and pooled befor normalization based on Quant-iT PicoGreen dsDNA Assay Kit DNA quantification. 500 0 Application Read Forward 1 1 Application Read Reverse 251 Illumina MiSeq PRJDB5471 SAMD00071633 S5-Sub-3 AMPLICON METAGENOMIC PCR PCR using barcoded Illumina primers was performed by following the Earth Microbiome protocols (Caporaso et al. 2012), with the following differences: HF Phusion polymerase mix (New England BioLabs, Ipswich, MA) was used and 3% dimethylsulfoxide (DMSO) was added to the reaction mixtures before cycling at 98????????????????????????????????C for 45 s, 50????????????????????????????????C for 60 s, and 72????????????????????????????????C for 90 s. Amplifications for each sample were performed in triplicate and pooled befor normalization based on Quant-iT PicoGreen dsDNA Assay Kit DNA quantification. 500 0 Application Read Forward 1 1 Application Read Reverse 251 Illumina MiSeq PRJDB5471 SAMD00071633 S5-Sub-4 AMPLICON METAGENOMIC PCR PCR using barcoded Illumina primers was performed by following the Earth Microbiome protocols (Caporaso et al. 2012), with the following differences: HF Phusion polymerase mix (New England BioLabs, Ipswich, MA) was used and 3% dimethylsulfoxide (DMSO) was added to the reaction mixtures before cycling at 98????????????????????????????????C for 45 s, 50????????????????????????????????C for 60 s, and 72????????????????????????????????C for 90 s. Amplifications for each sample were performed in triplicate and pooled befor normalization based on Quant-iT PicoGreen dsDNA Assay Kit DNA quantification. 500 0 Application Read Forward 1 1 Application Read Reverse 251 Illumina MiSeq PRJDB5471 SAMD00071633 S5-Sub-5 AMPLICON METAGENOMIC PCR PCR using barcoded Illumina primers was performed by following the Earth Microbiome protocols (Caporaso et al. 2012), with the following differences: HF Phusion polymerase mix (New England BioLabs, Ipswich, MA) was used and 3% dimethylsulfoxide (DMSO) was added to the reaction mixtures before cycling at 98????????????????????????????????C for 45 s, 50????????????????????????????????C for 60 s, and 72????????????????????????????????C for 90 s. Amplifications for each sample were performed in triplicate and pooled befor normalization based on Quant-iT PicoGreen dsDNA Assay Kit DNA quantification. 500 0 Application Read Forward 1 1 Application Read Reverse 251 Illumina MiSeq PRJDB5471 SAMD00071652 S6-Mac-1 AMPLICON METAGENOMIC PCR PCR using barcoded Illumina primers was performed by following the Earth Microbiome protocols (Caporaso et al. 2012), with the following differences: HF Phusion polymerase mix (New England BioLabs, Ipswich, MA) was used and 3% dimethylsulfoxide (DMSO) was added to the reaction mixtures before cycling at 98????????????????????????????????C for 45 s, 50????????????????????????????????C for 60 s, and 72????????????????????????????????C for 90 s. Amplifications for each sample were performed in triplicate and pooled befor normalization based on Quant-iT PicoGreen dsDNA Assay Kit DNA quantification. 500 0 Application Read Forward 1 1 Application Read Reverse 251 Illumina MiSeq PRJDB5471 SAMD00071652 S6-Mac-2 AMPLICON METAGENOMIC PCR PCR using barcoded Illumina primers was performed by following the Earth Microbiome protocols (Caporaso et al. 2012), with the following differences: HF Phusion polymerase mix (New England BioLabs, Ipswich, MA) was used and 3% dimethylsulfoxide (DMSO) was added to the reaction mixtures before cycling at 98????????????????????????????????C for 45 s, 50????????????????????????????????C for 60 s, and 72????????????????????????????????C for 90 s. Amplifications for each sample were performed in triplicate and pooled befor normalization based on Quant-iT PicoGreen dsDNA Assay Kit DNA quantification. 500 0 Application Read Forward 1 1 Application Read Reverse 251 Illumina MiSeq PRJDB5471 SAMD00071652 S6-Mac-3 AMPLICON METAGENOMIC PCR PCR using barcoded Illumina primers was performed by following the Earth Microbiome protocols (Caporaso et al. 2012), with the following differences: HF Phusion polymerase mix (New England BioLabs, Ipswich, MA) was used and 3% dimethylsulfoxide (DMSO) was added to the reaction mixtures before cycling at 98????????????????????????????????C for 45 s, 50????????????????????????????????C for 60 s, and 72????????????????????????????????C for 90 s. Amplifications for each sample were performed in triplicate and pooled befor normalization based on Quant-iT PicoGreen dsDNA Assay Kit DNA quantification. 500 0 Application Read Forward 1 1 Application Read Reverse 251 Illumina MiSeq PRJDB5471 SAMD00071652 S6-Mac-4 AMPLICON METAGENOMIC PCR PCR using barcoded Illumina primers was performed by following the Earth Microbiome protocols (Caporaso et al. 2012), with the following differences: HF Phusion polymerase mix (New England BioLabs, Ipswich, MA) was used and 3% dimethylsulfoxide (DMSO) was added to the reaction mixtures before cycling at 98????????????????????????????????C for 45 s, 50????????????????????????????????C for 60 s, and 72????????????????????????????????C for 90 s. Amplifications for each sample were performed in triplicate and pooled befor normalization based on Quant-iT PicoGreen dsDNA Assay Kit DNA quantification. 500 0 Application Read Forward 1 1 Application Read Reverse 251 Illumina MiSeq PRJDB5471 SAMD00071652 S6-Mac-5 AMPLICON METAGENOMIC PCR PCR using barcoded Illumina primers was performed by following the Earth Microbiome protocols (Caporaso et al. 2012), with the following differences: HF Phusion polymerase mix (New England BioLabs, Ipswich, MA) was used and 3% dimethylsulfoxide (DMSO) was added to the reaction mixtures before cycling at 98????????????????????????????????C for 45 s, 50????????????????????????????????C for 60 s, and 72????????????????????????????????C for 90 s. Amplifications for each sample were performed in triplicate and pooled befor normalization based on Quant-iT PicoGreen dsDNA Assay Kit DNA quantification. 500 0 Application Read Forward 1 1 Application Read Reverse 251 Illumina MiSeq PRJDB5471 SAMD00071653 S6-Ric-1 AMPLICON METAGENOMIC PCR PCR using barcoded Illumina primers was performed by following the Earth Microbiome protocols (Caporaso et al. 2012), with the following differences: HF Phusion polymerase mix (New England BioLabs, Ipswich, MA) was used and 3% dimethylsulfoxide (DMSO) was added to the reaction mixtures before cycling at 98????????????????????????????????C for 45 s, 50????????????????????????????????C for 60 s, and 72????????????????????????????????C for 90 s. Amplifications for each sample were performed in triplicate and pooled befor normalization based on Quant-iT PicoGreen dsDNA Assay Kit DNA quantification. 500 0 Application Read Forward 1 1 Application Read Reverse 251 Illumina MiSeq PRJDB5471 SAMD00071653 S6-Ric-2 AMPLICON METAGENOMIC PCR PCR using barcoded Illumina primers was performed by following the Earth Microbiome protocols (Caporaso et al. 2012), with the following differences: HF Phusion polymerase mix (New England BioLabs, Ipswich, MA) was used and 3% dimethylsulfoxide (DMSO) was added to the reaction mixtures before cycling at 98????????????????????????????????C for 45 s, 50????????????????????????????????C for 60 s, and 72????????????????????????????????C for 90 s. Amplifications for each sample were performed in triplicate and pooled befor normalization based on Quant-iT PicoGreen dsDNA Assay Kit DNA quantification. 500 0 Application Read Forward 1 1 Application Read Reverse 251 Illumina MiSeq PRJDB5471 SAMD00071653 S6-Ric-3 AMPLICON METAGENOMIC PCR PCR using barcoded Illumina primers was performed by following the Earth Microbiome protocols (Caporaso et al. 2012), with the following differences: HF Phusion polymerase mix (New England BioLabs, Ipswich, MA) was used and 3% dimethylsulfoxide (DMSO) was added to the reaction mixtures before cycling at 98????????????????????????????????C for 45 s, 50????????????????????????????????C for 60 s, and 72????????????????????????????????C for 90 s. Amplifications for each sample were performed in triplicate and pooled befor normalization based on Quant-iT PicoGreen dsDNA Assay Kit DNA quantification. 500 0 Application Read Forward 1 1 Application Read Reverse 251 Illumina MiSeq PRJDB5471 SAMD00071653 S6-Ric-4 AMPLICON METAGENOMIC PCR PCR using barcoded Illumina primers was performed by following the Earth Microbiome protocols (Caporaso et al. 2012), with the following differences: HF Phusion polymerase mix (New England BioLabs, Ipswich, MA) was used and 3% dimethylsulfoxide (DMSO) was added to the reaction mixtures before cycling at 98????????????????????????????????C for 45 s, 50????????????????????????????????C for 60 s, and 72????????????????????????????????C for 90 s. Amplifications for each sample were performed in triplicate and pooled befor normalization based on Quant-iT PicoGreen dsDNA Assay Kit DNA quantification. 500 0 Application Read Forward 1 1 Application Read Reverse 251 Illumina MiSeq PRJDB5471 SAMD00071653 S6-Ric-5 AMPLICON METAGENOMIC PCR PCR using barcoded Illumina primers was performed by following the Earth Microbiome protocols (Caporaso et al. 2012), with the following differences: HF Phusion polymerase mix (New England BioLabs, Ipswich, MA) was used and 3% dimethylsulfoxide (DMSO) was added to the reaction mixtures before cycling at 98????????????????????????????????C for 45 s, 50????????????????????????????????C for 60 s, and 72????????????????????????????????C for 90 s. Amplifications for each sample were performed in triplicate and pooled befor normalization based on Quant-iT PicoGreen dsDNA Assay Kit DNA quantification. 500 0 Application Read Forward 1 1 Application Read Reverse 251 Illumina MiSeq PRJDB5471 SAMD00071654 S6-Sub-1 AMPLICON METAGENOMIC PCR PCR using barcoded Illumina primers was performed by following the Earth Microbiome protocols (Caporaso et al. 2012), with the following differences: HF Phusion polymerase mix (New England BioLabs, Ipswich, MA) was used and 3% dimethylsulfoxide (DMSO) was added to the reaction mixtures before cycling at 98????????????????????????????????C for 45 s, 50????????????????????????????????C for 60 s, and 72????????????????????????????????C for 90 s. Amplifications for each sample were performed in triplicate and pooled befor normalization based on Quant-iT PicoGreen dsDNA Assay Kit DNA quantification. 500 0 Application Read Forward 1 1 Application Read Reverse 251 Illumina MiSeq PRJDB5471 SAMD00071654 S6-Sub-2 AMPLICON METAGENOMIC PCR PCR using barcoded Illumina primers was performed by following the Earth Microbiome protocols (Caporaso et al. 2012), with the following differences: HF Phusion polymerase mix (New England BioLabs, Ipswich, MA) was used and 3% dimethylsulfoxide (DMSO) was added to the reaction mixtures before cycling at 98????????????????????????????????C for 45 s, 50????????????????????????????????C for 60 s, and 72????????????????????????????????C for 90 s. Amplifications for each sample were performed in triplicate and pooled befor normalization based on Quant-iT PicoGreen dsDNA Assay Kit DNA quantification. 500 0 Application Read Forward 1 1 Application Read Reverse 251 Illumina MiSeq PRJDB5471 SAMD00071654 S6-Sub-3 AMPLICON METAGENOMIC PCR PCR using barcoded Illumina primers was performed by following the Earth Microbiome protocols (Caporaso et al. 2012), with the following differences: HF Phusion polymerase mix (New England BioLabs, Ipswich, MA) was used and 3% dimethylsulfoxide (DMSO) was added to the reaction mixtures before cycling at 98????????????????????????????????C for 45 s, 50????????????????????????????????C for 60 s, and 72????????????????????????????????C for 90 s. Amplifications for each sample were performed in triplicate and pooled befor normalization based on Quant-iT PicoGreen dsDNA Assay Kit DNA quantification. 500 0 Application Read Forward 1 1 Application Read Reverse 251 Illumina MiSeq PRJDB5471 SAMD00071654 S6-Sub-4 AMPLICON METAGENOMIC PCR PCR using barcoded Illumina primers was performed by following the Earth Microbiome protocols (Caporaso et al. 2012), with the following differences: HF Phusion polymerase mix (New England BioLabs, Ipswich, MA) was used and 3% dimethylsulfoxide (DMSO) was added to the reaction mixtures before cycling at 98????????????????????????????????C for 45 s, 50????????????????????????????????C for 60 s, and 72????????????????????????????????C for 90 s. Amplifications for each sample were performed in triplicate and pooled befor normalization based on Quant-iT PicoGreen dsDNA Assay Kit DNA quantification. 500 0 Application Read Forward 1 1 Application Read Reverse 251 Illumina MiSeq PRJDB5471 SAMD00071654 S6-Sub-5 AMPLICON METAGENOMIC PCR PCR using barcoded Illumina primers was performed by following the Earth Microbiome protocols (Caporaso et al. 2012), with the following differences: HF Phusion polymerase mix (New England BioLabs, Ipswich, MA) was used and 3% dimethylsulfoxide (DMSO) was added to the reaction mixtures before cycling at 98????????????????????????????????C for 45 s, 50????????????????????????????????C for 60 s, and 72????????????????????????????????C for 90 s. Amplifications for each sample were performed in triplicate and pooled befor normalization based on Quant-iT PicoGreen dsDNA Assay Kit DNA quantification. 500 0 Application Read Forward 1 1 Application Read Reverse 251 Illumina MiSeq PRJDB5471 SAMD00071655 S7-Ech-1 AMPLICON METAGENOMIC PCR PCR using barcoded Illumina primers was performed by following the Earth Microbiome protocols (Caporaso et al. 2012), with the following differences: HF Phusion polymerase mix (New England BioLabs, Ipswich, MA) was used and 3% dimethylsulfoxide (DMSO) was added to the reaction mixtures before cycling at 98????????????????????????????????C for 45 s, 50????????????????????????????????C for 60 s, and 72????????????????????????????????C for 90 s. Amplifications for each sample were performed in triplicate and pooled befor normalization based on Quant-iT PicoGreen dsDNA Assay Kit DNA quantification. 500 0 Application Read Forward 1 1 Application Read Reverse 251 Illumina MiSeq PRJDB5471 SAMD00071655 S7-Ech-2 AMPLICON METAGENOMIC PCR PCR using barcoded Illumina primers was performed by following the Earth Microbiome protocols (Caporaso et al. 2012), with the following differences: HF Phusion polymerase mix (New England BioLabs, Ipswich, MA) was used and 3% dimethylsulfoxide (DMSO) was added to the reaction mixtures before cycling at 98????????????????????????????????C for 45 s, 50????????????????????????????????C for 60 s, and 72????????????????????????????????C for 90 s. Amplifications for each sample were performed in triplicate and pooled befor normalization based on Quant-iT PicoGreen dsDNA Assay Kit DNA quantification. 500 0 Application Read Forward 1 1 Application Read Reverse 251 Illumina MiSeq PRJDB5471 SAMD00071655 S7-Ech-3 AMPLICON METAGENOMIC PCR PCR using barcoded Illumina primers was performed by following the Earth Microbiome protocols (Caporaso et al. 2012), with the following differences: HF Phusion polymerase mix (New England BioLabs, Ipswich, MA) was used and 3% dimethylsulfoxide (DMSO) was added to the reaction mixtures before cycling at 98????????????????????????????????C for 45 s, 50????????????????????????????????C for 60 s, and 72????????????????????????????????C for 90 s. Amplifications for each sample were performed in triplicate and pooled befor normalization based on Quant-iT PicoGreen dsDNA Assay Kit DNA quantification. 500 0 Application Read Forward 1 1 Application Read Reverse 251 Illumina MiSeq PRJDB5471 SAMD00071655 S7-Ech-4 AMPLICON METAGENOMIC PCR PCR using barcoded Illumina primers was performed by following the Earth Microbiome protocols (Caporaso et al. 2012), with the following differences: HF Phusion polymerase mix (New England BioLabs, Ipswich, MA) was used and 3% dimethylsulfoxide (DMSO) was added to the reaction mixtures before cycling at 98????????????????????????????????C for 45 s, 50????????????????????????????????C for 60 s, and 72????????????????????????????????C for 90 s. Amplifications for each sample were performed in triplicate and pooled befor normalization based on Quant-iT PicoGreen dsDNA Assay Kit DNA quantification. 500 0 Application Read Forward 1 1 Application Read Reverse 251 Illumina MiSeq PRJDB5471 SAMD00071655 S7-Ech-5 AMPLICON METAGENOMIC PCR PCR using barcoded Illumina primers was performed by following the Earth Microbiome protocols (Caporaso et al. 2012), with the following differences: HF Phusion polymerase mix (New England BioLabs, Ipswich, MA) was used and 3% dimethylsulfoxide (DMSO) was added to the reaction mixtures before cycling at 98????????????????????????????????C for 45 s, 50????????????????????????????????C for 60 s, and 72????????????????????????????????C for 90 s. Amplifications for each sample were performed in triplicate and pooled befor normalization based on Quant-iT PicoGreen dsDNA Assay Kit DNA quantification. 500 0 Application Read Forward 1 1 Application Read Reverse 251 Illumina MiSeq PRJDB5471 SAMD00071656 S7-Par-1 AMPLICON METAGENOMIC PCR PCR using barcoded Illumina primers was performed by following the Earth Microbiome protocols (Caporaso et al. 2012), with the following differences: HF Phusion polymerase mix (New England BioLabs, Ipswich, MA) was used and 3% dimethylsulfoxide (DMSO) was added to the reaction mixtures before cycling at 98????????????????????????????????C for 45 s, 50????????????????????????????????C for 60 s, and 72????????????????????????????????C for 90 s. Amplifications for each sample were performed in triplicate and pooled befor normalization based on Quant-iT PicoGreen dsDNA Assay Kit DNA quantification. 500 0 Application Read Forward 1 1 Application Read Reverse 251 Illumina MiSeq PRJDB5471 SAMD00071656 S7-Par-2 AMPLICON METAGENOMIC PCR PCR using barcoded Illumina primers was performed by following the Earth Microbiome protocols (Caporaso et al. 2012), with the following differences: HF Phusion polymerase mix (New England BioLabs, Ipswich, MA) was used and 3% dimethylsulfoxide (DMSO) was added to the reaction mixtures before cycling at 98????????????????????????????????C for 45 s, 50????????????????????????????????C for 60 s, and 72????????????????????????????????C for 90 s. Amplifications for each sample were performed in triplicate and pooled befor normalization based on Quant-iT PicoGreen dsDNA Assay Kit DNA quantification. 500 0 Application Read Forward 1 1 Application Read Reverse 251 Illumina MiSeq PRJDB5471 SAMD00071656 S7-Par-3 AMPLICON METAGENOMIC PCR PCR using barcoded Illumina primers was performed by following the Earth Microbiome protocols (Caporaso et al. 2012), with the following differences: HF Phusion polymerase mix (New England BioLabs, Ipswich, MA) was used and 3% dimethylsulfoxide (DMSO) was added to the reaction mixtures before cycling at 98????????????????????????????????C for 45 s, 50????????????????????????????????C for 60 s, and 72????????????????????????????????C for 90 s. Amplifications for each sample were performed in triplicate and pooled befor normalization based on Quant-iT PicoGreen dsDNA Assay Kit DNA quantification. 500 0 Application Read Forward 1 1 Application Read Reverse 251 Illumina MiSeq PRJDB5471 SAMD00071656 S7-Par-4 AMPLICON METAGENOMIC PCR PCR using barcoded Illumina primers was performed by following the Earth Microbiome protocols (Caporaso et al. 2012), with the following differences: HF Phusion polymerase mix (New England BioLabs, Ipswich, MA) was used and 3% dimethylsulfoxide (DMSO) was added to the reaction mixtures before cycling at 98????????????????????????????????C for 45 s, 50????????????????????????????????C for 60 s, and 72????????????????????????????????C for 90 s. Amplifications for each sample were performed in triplicate and pooled befor normalization based on Quant-iT PicoGreen dsDNA Assay Kit DNA quantification. 500 0 Application Read Forward 1 1 Application Read Reverse 251 Illumina MiSeq PRJDB5471 SAMD00071656 S7-Par-5 AMPLICON METAGENOMIC PCR PCR using barcoded Illumina primers was performed by following the Earth Microbiome protocols (Caporaso et al. 2012), with the following differences: HF Phusion polymerase mix (New England BioLabs, Ipswich, MA) was used and 3% dimethylsulfoxide (DMSO) was added to the reaction mixtures before cycling at 98????????????????????????????????C for 45 s, 50????????????????????????????????C for 60 s, and 72????????????????????????????????C for 90 s. Amplifications for each sample were performed in triplicate and pooled befor normalization based on Quant-iT PicoGreen dsDNA Assay Kit DNA quantification. 500 0 Application Read Forward 1 1 Application Read Reverse 251 Illumina MiSeq PRJDB5471 SAMD00071657 S7-Sub-1 AMPLICON METAGENOMIC PCR PCR using barcoded Illumina primers was performed by following the Earth Microbiome protocols (Caporaso et al. 2012), with the following differences: HF Phusion polymerase mix (New England BioLabs, Ipswich, MA) was used and 3% dimethylsulfoxide (DMSO) was added to the reaction mixtures before cycling at 98????????????????????????????????C for 45 s, 50????????????????????????????????C for 60 s, and 72????????????????????????????????C for 90 s. Amplifications for each sample were performed in triplicate and pooled befor normalization based on Quant-iT PicoGreen dsDNA Assay Kit DNA quantification. 500 0 Application Read Forward 1 1 Application Read Reverse 251 Illumina MiSeq PRJDB5471 SAMD00071657 S7-Sub-2 AMPLICON METAGENOMIC PCR PCR using barcoded Illumina primers was performed by following the Earth Microbiome protocols (Caporaso et al. 2012), with the following differences: HF Phusion polymerase mix (New England BioLabs, Ipswich, MA) was used and 3% dimethylsulfoxide (DMSO) was added to the reaction mixtures before cycling at 98????????????????????????????????C for 45 s, 50????????????????????????????????C for 60 s, and 72????????????????????????????????C for 90 s. Amplifications for each sample were performed in triplicate and pooled befor normalization based on Quant-iT PicoGreen dsDNA Assay Kit DNA quantification. 500 0 Application Read Forward 1 1 Application Read Reverse 251 Illumina MiSeq PRJDB5471 SAMD00071657 S7-Sub-3 AMPLICON METAGENOMIC PCR PCR using barcoded Illumina primers was performed by following the Earth Microbiome protocols (Caporaso et al. 2012), with the following differences: HF Phusion polymerase mix (New England BioLabs, Ipswich, MA) was used and 3% dimethylsulfoxide (DMSO) was added to the reaction mixtures before cycling at 98????????????????????????????????C for 45 s, 50????????????????????????????????C for 60 s, and 72????????????????????????????????C for 90 s. Amplifications for each sample were performed in triplicate and pooled befor normalization based on Quant-iT PicoGreen dsDNA Assay Kit DNA quantification. 500 0 Application Read Forward 1 1 Application Read Reverse 251 Illumina MiSeq PRJDB5471 SAMD00071657 S7-Sub-4 AMPLICON METAGENOMIC PCR PCR using barcoded Illumina primers was performed by following the Earth Microbiome protocols (Caporaso et al. 2012), with the following differences: HF Phusion polymerase mix (New England BioLabs, Ipswich, MA) was used and 3% dimethylsulfoxide (DMSO) was added to the reaction mixtures before cycling at 98????????????????????????????????C for 45 s, 50????????????????????????????????C for 60 s, and 72????????????????????????????????C for 90 s. Amplifications for each sample were performed in triplicate and pooled befor normalization based on Quant-iT PicoGreen dsDNA Assay Kit DNA quantification. 500 0 Application Read Forward 1 1 Application Read Reverse 251 Illumina MiSeq PRJDB5471 SAMD00071657 S7-Sub-5 AMPLICON METAGENOMIC PCR PCR using barcoded Illumina primers was performed by following the Earth Microbiome protocols (Caporaso et al. 2012), with the following differences: HF Phusion polymerase mix (New England BioLabs, Ipswich, MA) was used and 3% dimethylsulfoxide (DMSO) was added to the reaction mixtures before cycling at 98????????????????????????????????C for 45 s, 50????????????????????????????????C for 60 s, and 72????????????????????????????????C for 90 s. Amplifications for each sample were performed in triplicate and pooled befor normalization based on Quant-iT PicoGreen dsDNA Assay Kit DNA quantification. 500 0 Application Read Forward 1 1 Application Read Reverse 251 Illumina MiSeq PRJDB5471 SAMD00071658 control-1 AMPLICON METAGENOMIC PCR PCR using barcoded Illumina primers was performed by following the Earth Microbiome protocols (Caporaso et al. 2012), with the following differences: HF Phusion polymerase mix (New England BioLabs, Ipswich, MA) was used and 3% dimethylsulfoxide (DMSO) was added to the reaction mixtures before cycling at 98????????????????????????????????C for 45 s, 50????????????????????????????????C for 60 s, and 72????????????????????????????????C for 90 s. Amplifications for each sample were performed in triplicate and pooled befor normalization based on Quant-iT PicoGreen dsDNA Assay Kit DNA quantification. 500 0 Application Read Forward 1 1 Application Read Reverse 251 Illumina MiSeq PRJDB5471 SAMD00071659 control-2 AMPLICON METAGENOMIC PCR PCR using barcoded Illumina primers was performed by following the Earth Microbiome protocols (Caporaso et al. 2012), with the following differences: HF Phusion polymerase mix (New England BioLabs, Ipswich, MA) was used and 3% dimethylsulfoxide (DMSO) was added to the reaction mixtures before cycling at 98????????????????????????????????C for 45 s, 50????????????????????????????????C for 60 s, and 72????????????????????????????????C for 90 s. Amplifications for each sample were performed in triplicate and pooled befor normalization based on Quant-iT PicoGreen dsDNA Assay Kit DNA quantification. 500 0 Application Read Forward 1 1 Application Read Reverse 251 Illumina MiSeq