PRJDB3186 SAMD00078265 WGS GENOMIC RANDOM We obtained 301bp x 2 pair-end reads. The pair-end reads were imported into CLC genomics workbench, a commercial WGS data analysis software (QIAGEN), During the imported process, the 2 fastq data files of pair end reads were merged into one files. The reads were trimmed based on the Qv values (Qv20,) and the length (reads in the length in less than 16bp were omitted). After trimming, the reads data were exported as a single fastq file. Thus we registered the fastq data as single read fastq. 287 0 Application Read Forward 1 Illumina MiSeq PRJDB3186 SAMD00078266 WGS GENOMIC RANDOM We obtained 301bp x 2 pair-end reads. The pair-end reads were imported into CLC genomics workbench, a commercial WGS data analysis software (QIAGEN), During the imported process, the 2 fastq data files of pair end reads were merged into one files. The reads were trimmed based on the Qv values (Qv20,) and the length (reads in the length in less than 16bp were omitted). After trimming, the reads data were exported as a single fastq file. Thus we registered the fastq data as single read fastq. 287 0 Application Read Forward 1 Illumina MiSeq PRJDB3186 SAMD00078267 WGS GENOMIC RANDOM We obtained 301bp x 2 pair-end reads. The pair-end reads were imported into CLC genomics workbench, a commercial WGS data analysis software (QIAGEN), During the imported process, the 2 fastq data files of pair end reads were merged into one files. The reads were trimmed based on the Qv values (Qv20,) and the length (reads in the length in less than 16bp were omitted). After trimming, the reads data were exported as a single fastq file. Thus we registered the fastq data as single read fastq. 287 0 Application Read Forward 1 Illumina MiSeq PRJDB3186 SAMD00078268 WGS GENOMIC RANDOM We obtained 301bp x 2 pair-end reads. The pair-end reads were imported into CLC genomics workbench, a commercial WGS data analysis software (QIAGEN), During the imported process, the 2 fastq data files of pair end reads were merged into one files. The reads were trimmed based on the Qv values (Qv20,) and the length (reads in the length in less than 16bp were omitted). After trimming, the reads data were exported as a single fastq file. Thus we registered the fastq data as single read fastq. 287 0 Application Read Forward 1 Illumina MiSeq PRJDB3186 SAMD00078269 WGS GENOMIC RANDOM We obtained 301bp x 2 pair-end reads. The pair-end reads were imported into CLC genomics workbench, a commercial WGS data analysis software (QIAGEN), During the imported process, the 2 fastq data files of pair end reads were merged into one files. The reads were trimmed based on the Qv values (Qv20,) and the length (reads in the length in less than 16bp were omitted). After trimming, the reads data were exported as a single fastq file. Thus we registered the fastq data as single read fastq. 287 0 Application Read Forward 1 Illumina MiSeq PRJDB3186 SAMD00078270 WGS GENOMIC RANDOM We obtained 301bp x 2 pair-end reads. The pair-end reads were imported into CLC genomics workbench, a commercial WGS data analysis software (QIAGEN), During the imported process, the 2 fastq data files of pair end reads were merged into one files. The reads were trimmed based on the Qv values (Qv20,) and the length (reads in the length in less than 16bp were omitted). After trimming, the reads data were exported as a single fastq file. Thus we registered the fastq data as single read fastq. 287 0 Application Read Forward 1 Illumina MiSeq PRJDB3186 SAMD00078271 WGS GENOMIC RANDOM We obtained 301bp x 2 pair-end reads. The pair-end reads were imported into CLC genomics workbench, a commercial WGS data analysis software (QIAGEN), During the imported process, the 2 fastq data files of pair end reads were merged into one files. The reads were trimmed based on the Qv values (Qv20,) and the length (reads in the length in less than 16bp were omitted). After trimming, the reads data were exported as a single fastq file. Thus we registered the fastq data as single read fastq. 287 0 Application Read Forward 1 Illumina MiSeq PRJDB3186 SAMD00078272 WGS GENOMIC RANDOM We obtained 301bp x 2 pair-end reads. The pair-end reads were imported into CLC genomics workbench, a commercial WGS data analysis software (QIAGEN), During the imported process, the 2 fastq data files of pair end reads were merged into one files. The reads were trimmed based on the Qv values (Qv20,) and the length (reads in the length in less than 16bp were omitted). After trimming, the reads data were exported as a single fastq file. Thus we registered the fastq data as single read fastq. 287 0 Application Read Forward 1 Illumina MiSeq PRJDB3186 SAMD00078273 WGS GENOMIC RANDOM We obtained 301bp x 2 pair-end reads. The pair-end reads were imported into CLC genomics workbench, a commercial WGS data analysis software (QIAGEN), During the imported process, the 2 fastq data files of pair end reads were merged into one files. The reads were trimmed based on the Qv values (Qv20,) and the length (reads in the length in less than 16bp were omitted). After trimming, the reads data were exported as a single fastq file. Thus we registered the fastq data as single read fastq. 287 0 Application Read Forward 1 Illumina MiSeq PRJDB3186 SAMD00078274 WGS GENOMIC RANDOM We obtained 301bp x 2 pair-end reads. The pair-end reads were imported into CLC genomics workbench, a commercial WGS data analysis software (QIAGEN), During the imported process, the 2 fastq data files of pair end reads were merged into one files. The reads were trimmed based on the Qv values (Qv20,) and the length (reads in the length in less than 16bp were omitted). After trimming, the reads data were exported as a single fastq file. Thus we registered the fastq data as single read fastq. 287 0 Application Read Forward 1 Illumina MiSeq PRJDB3186 SAMD00078275 WGS GENOMIC RANDOM We obtained 301bp x 2 pair-end reads. The pair-end reads were imported into CLC genomics workbench, a commercial WGS data analysis software (QIAGEN), During the imported process, the 2 fastq data files of pair end reads were merged into one files. The reads were trimmed based on the Qv values (Qv20,) and the length (reads in the length in less than 16bp were omitted). After trimming, the reads data were exported as a single fastq file. Thus we registered the fastq data as single read fastq. 287 0 Application Read Forward 1 Illumina MiSeq PRJDB3186 SAMD00078276 WGS GENOMIC RANDOM We obtained 301bp x 2 pair-end reads. The pair-end reads were imported into CLC genomics workbench, a commercial WGS data analysis software (QIAGEN), During the imported process, the 2 fastq data files of pair end reads were merged into one files. The reads were trimmed based on the Qv values (Qv20,) and the length (reads in the length in less than 16bp were omitted). After trimming, the reads data were exported as a single fastq file. Thus we registered the fastq data as single read fastq. 287 0 Application Read Forward 1 Illumina MiSeq PRJDB3186 SAMD00078277 WGS GENOMIC RANDOM We obtained 301bp x 2 pair-end reads. The pair-end reads were imported into CLC genomics workbench, a commercial WGS data analysis software (QIAGEN), During the imported process, the 2 fastq data files of pair end reads were merged into one files. The reads were trimmed based on the Qv values (Qv20,) and the length (reads in the length in less than 16bp were omitted). After trimming, the reads data were exported as a single fastq file. Thus we registered the fastq data as single read fastq. 287 0 Application Read Forward 1 Illumina MiSeq PRJDB3186 SAMD00078278 WGS GENOMIC RANDOM We obtained 301bp x 2 pair-end reads. The pair-end reads were imported into CLC genomics workbench, a commercial WGS data analysis software (QIAGEN), During the imported process, the 2 fastq data files of pair end reads were merged into one files. The reads were trimmed based on the Qv values (Qv20,) and the length (reads in the length in less than 16bp were omitted). After trimming, the reads data were exported as a single fastq file. Thus we registered the fastq data as single read fastq. 287 0 Application Read Forward 1 Illumina MiSeq PRJDB3186 SAMD00078279 WGS GENOMIC RANDOM We obtained 301bp x 2 pair-end reads. The pair-end reads were imported into CLC genomics workbench, a commercial WGS data analysis software (QIAGEN), During the imported process, the 2 fastq data files of pair end reads were merged into one files. The reads were trimmed based on the Qv values (Qv20,) and the length (reads in the length in less than 16bp were omitted). After trimming, the reads data were exported as a single fastq file. Thus we registered the fastq data as single read fastq. 287 0 Application Read Forward 1 Illumina MiSeq PRJDB3186 SAMD00078280 WGS GENOMIC RANDOM We obtained 301bp x 2 pair-end reads. The pair-end reads were imported into CLC genomics workbench, a commercial WGS data analysis software (QIAGEN), During the imported process, the 2 fastq data files of pair end reads were merged into one files. The reads were trimmed based on the Qv values (Qv20,) and the length (reads in the length in less than 16bp were omitted). After trimming, the reads data were exported as a single fastq file. Thus we registered the fastq data as single read fastq. 287 0 Application Read Forward 1 Illumina MiSeq PRJDB3186 SAMD00078281 WGS GENOMIC RANDOM We obtained 301bp x 2 pair-end reads. The pair-end reads were imported into CLC genomics workbench, a commercial WGS data analysis software (QIAGEN), During the imported process, the 2 fastq data files of pair end reads were merged into one files. The reads were trimmed based on the Qv values (Qv20,) and the length (reads in the length in less than 16bp were omitted). After trimming, the reads data were exported as a single fastq file. Thus we registered the fastq data as single read fastq. 287 0 Application Read Forward 1 Illumina MiSeq PRJDB3186 SAMD00078282 WGS GENOMIC RANDOM We obtained 301bp x 2 pair-end reads. The pair-end reads were imported into CLC genomics workbench, a commercial WGS data analysis software (QIAGEN), During the imported process, the 2 fastq data files of pair end reads were merged into one files. The reads were trimmed based on the Qv values (Qv20,) and the length (reads in the length in less than 16bp were omitted). After trimming, the reads data were exported as a single fastq file. Thus we registered the fastq data as single read fastq. 287 0 Application Read Forward 1 Illumina MiSeq PRJDB3186 SAMD00078283 WGS GENOMIC RANDOM We obtained 301bp x 2 pair-end reads. The pair-end reads were imported into CLC genomics workbench, a commercial WGS data analysis software (QIAGEN), During the imported process, the 2 fastq data files of pair end reads were merged into one files. The reads were trimmed based on the Qv values (Qv20,) and the length (reads in the length in less than 16bp were omitted). After trimming, the reads data were exported as a single fastq file. Thus we registered the fastq data as single read fastq. 287 0 Application Read Forward 1 Illumina MiSeq PRJDB3186 SAMD00078284 WGS GENOMIC RANDOM We obtained 301bp x 2 pair-end reads. The pair-end reads were imported into CLC genomics workbench, a commercial WGS data analysis software (QIAGEN), During the imported process, the 2 fastq data files of pair end reads were merged into one files. The reads were trimmed based on the Qv values (Qv20,) and the length (reads in the length in less than 16bp were omitted). After trimming, the reads data were exported as a single fastq file. Thus we registered the fastq data as single read fastq. 287 0 Application Read Forward 1 Illumina MiSeq PRJDB3186 SAMD00078285 WGS GENOMIC RANDOM We obtained 301bp x 2 pair-end reads. The pair-end reads were imported into CLC genomics workbench, a commercial WGS data analysis software (QIAGEN), During the imported process, the 2 fastq data files of pair end reads were merged into one files. The reads were trimmed based on the Qv values (Qv20,) and the length (reads in the length in less than 16bp were omitted). After trimming, the reads data were exported as a single fastq file. Thus we registered the fastq data as single read fastq. 287 0 Application Read Forward 1 Illumina MiSeq PRJDB3186 SAMD00078286 WGS GENOMIC RANDOM We obtained 301bp x 2 pair-end reads. The pair-end reads were imported into CLC genomics workbench, a commercial WGS data analysis software (QIAGEN), During the imported process, the 2 fastq data files of pair end reads were merged into one files. The reads were trimmed based on the Qv values (Qv20,) and the length (reads in the length in less than 16bp were omitted). After trimming, the reads data were exported as a single fastq file. Thus we registered the fastq data as single read fastq. 287 0 Application Read Forward 1 Illumina MiSeq PRJDB3186 SAMD00078287 WGS GENOMIC RANDOM We obtained 301bp x 2 pair-end reads. The pair-end reads were imported into CLC genomics workbench, a commercial WGS data analysis software (QIAGEN), During the imported process, the 2 fastq data files of pair end reads were merged into one files. The reads were trimmed based on the Qv values (Qv20,) and the length (reads in the length in less than 16bp were omitted). After trimming, the reads data were exported as a single fastq file. Thus we registered the fastq data as single read fastq. 287 0 Application Read Forward 1 Illumina MiSeq PRJDB3186 SAMD00078288 WGS GENOMIC RANDOM We obtained 301bp x 2 pair-end reads. The pair-end reads were imported into CLC genomics workbench, a commercial WGS data analysis software (QIAGEN), During the imported process, the 2 fastq data files of pair end reads were merged into one files. The reads were trimmed based on the Qv values (Qv20,) and the length (reads in the length in less than 16bp were omitted). After trimming, the reads data were exported as a single fastq file. Thus we registered the fastq data as single read fastq. 287 0 Application Read Forward 1 Illumina MiSeq PRJDB3186 SAMD00078289 WGS GENOMIC RANDOM We obtained 301bp x 2 pair-end reads. The pair-end reads were imported into CLC genomics workbench, a commercial WGS data analysis software (QIAGEN), During the imported process, the 2 fastq data files of pair end reads were merged into one files. The reads were trimmed based on the Qv values (Qv20,) and the length (reads in the length in less than 16bp were omitted). After trimming, the reads data were exported as a single fastq file. Thus we registered the fastq data as single read fastq. 287 0 Application Read Forward 1 Illumina MiSeq PRJDB3186 SAMD00078290 WGS GENOMIC RANDOM We obtained 301bp x 2 pair-end reads. The pair-end reads were imported into CLC genomics workbench, a commercial WGS data analysis software (QIAGEN), During the imported process, the 2 fastq data files of pair end reads were merged into one files. The reads were trimmed based on the Qv values (Qv20,) and the length (reads in the length in less than 16bp were omitted). After trimming, the reads data were exported as a single fastq file. Thus we registered the fastq data as single read fastq. 287 0 Application Read Forward 1 Illumina MiSeq PRJDB3186 SAMD00078291 WGS GENOMIC RANDOM We obtained 301bp x 2 pair-end reads. The pair-end reads were imported into CLC genomics workbench, a commercial WGS data analysis software (QIAGEN), During the imported process, the 2 fastq data files of pair end reads were merged into one files. The reads were trimmed based on the Qv values (Qv20,) and the length (reads in the length in less than 16bp were omitted). After trimming, the reads data were exported as a single fastq file. Thus we registered the fastq data as single read fastq. 287 0 Application Read Forward 1 Illumina MiSeq PRJDB3186 SAMD00078292 WGS GENOMIC RANDOM We obtained 301bp x 2 pair-end reads. The pair-end reads were imported into CLC genomics workbench, a commercial WGS data analysis software (QIAGEN), During the imported process, the 2 fastq data files of pair end reads were merged into one files. The reads were trimmed based on the Qv values (Qv20,) and the length (reads in the length in less than 16bp were omitted). After trimming, the reads data were exported as a single fastq file. Thus we registered the fastq data as single read fastq. 287 0 Application Read Forward 1 Illumina MiSeq PRJDB3186 SAMD00078293 WGS GENOMIC RANDOM We obtained 301bp x 2 pair-end reads. The pair-end reads were imported into CLC genomics workbench, a commercial WGS data analysis software (QIAGEN), During the imported process, the 2 fastq data files of pair end reads were merged into one files. The reads were trimmed based on the Qv values (Qv20,) and the length (reads in the length in less than 16bp were omitted). After trimming, the reads data were exported as a single fastq file. Thus we registered the fastq data as single read fastq. 287 0 Application Read Forward 1 Illumina MiSeq PRJDB3186 SAMD00078294 WGS GENOMIC RANDOM We obtained 301bp x 2 pair-end reads. The pair-end reads were imported into CLC genomics workbench, a commercial WGS data analysis software (QIAGEN), During the imported process, the 2 fastq data files of pair end reads were merged into one files. The reads were trimmed based on the Qv values (Qv20,) and the length (reads in the length in less than 16bp were omitted). After trimming, the reads data were exported as a single fastq file. Thus we registered the fastq data as single read fastq. 287 0 Application Read Forward 1 Illumina MiSeq PRJDB3186 SAMD00078295 WGS GENOMIC RANDOM We obtained 301bp x 2 pair-end reads. The pair-end reads were imported into CLC genomics workbench, a commercial WGS data analysis software (QIAGEN), During the imported process, the 2 fastq data files of pair end reads were merged into one files. The reads were trimmed based on the Qv values (Qv20,) and the length (reads in the length in less than 16bp were omitted). After trimming, the reads data were exported as a single fastq file. Thus we registered the fastq data as single read fastq. 287 0 Application Read Forward 1 Illumina MiSeq PRJDB3186 SAMD00078296 WGS GENOMIC RANDOM We obtained 301bp x 2 pair-end reads. The pair-end reads were imported into CLC genomics workbench, a commercial WGS data analysis software (QIAGEN), During the imported process, the 2 fastq data files of pair end reads were merged into one files. The reads were trimmed based on the Qv values (Qv20,) and the length (reads in the length in less than 16bp were omitted). After trimming, the reads data were exported as a single fastq file. Thus we registered the fastq data as single read fastq. 287 0 Application Read Forward 1 Illumina MiSeq PRJDB3186 SAMD00078297 WGS GENOMIC RANDOM We obtained 301bp x 2 pair-end reads. The pair-end reads were imported into CLC genomics workbench, a commercial WGS data analysis software (QIAGEN), During the imported process, the 2 fastq data files of pair end reads were merged into one files. The reads were trimmed based on the Qv values (Qv20,) and the length (reads in the length in less than 16bp were omitted). After trimming, the reads data were exported as a single fastq file. Thus we registered the fastq data as single read fastq. 287 0 Application Read Forward 1 Illumina MiSeq PRJDB3186 SAMD00078298 WGS GENOMIC RANDOM We obtained 301bp x 2 pair-end reads. The pair-end reads were imported into CLC genomics workbench, a commercial WGS data analysis software (QIAGEN), During the imported process, the 2 fastq data files of pair end reads were merged into one files. The reads were trimmed based on the Qv values (Qv20,) and the length (reads in the length in less than 16bp were omitted). After trimming, the reads data were exported as a single fastq file. Thus we registered the fastq data as single read fastq. 287 0 Application Read Forward 1 Illumina MiSeq PRJDB3186 SAMD00078299 WGS GENOMIC RANDOM We obtained 301bp x 2 pair-end reads. The pair-end reads were imported into CLC genomics workbench, a commercial WGS data analysis software (QIAGEN), During the imported process, the 2 fastq data files of pair end reads were merged into one files. The reads were trimmed based on the Qv values (Qv20,) and the length (reads in the length in less than 16bp were omitted). After trimming, the reads data were exported as a single fastq file. Thus we registered the fastq data as single read fastq. 287 0 Application Read Forward 1 Illumina MiSeq PRJDB3186 SAMD00078300 WGS GENOMIC RANDOM We obtained 301bp x 2 pair-end reads. The pair-end reads were imported into CLC genomics workbench, a commercial WGS data analysis software (QIAGEN), During the imported process, the 2 fastq data files of pair end reads were merged into one files. The reads were trimmed based on the Qv values (Qv20,) and the length (reads in the length in less than 16bp were omitted). After trimming, the reads data were exported as a single fastq file. Thus we registered the fastq data as single read fastq. 287 0 Application Read Forward 1 Illumina MiSeq PRJDB3186 SAMD00078301 WGS GENOMIC RANDOM We obtained 301bp x 2 pair-end reads. The pair-end reads were imported into CLC genomics workbench, a commercial WGS data analysis software (QIAGEN), During the imported process, the 2 fastq data files of pair end reads were merged into one files. The reads were trimmed based on the Qv values (Qv20,) and the length (reads in the length in less than 16bp were omitted). After trimming, the reads data were exported as a single fastq file. Thus we registered the fastq data as single read fastq. 287 0 Application Read Forward 1 Illumina MiSeq PRJDB3186 SAMD00078302 WGS GENOMIC RANDOM We obtained 301bp x 2 pair-end reads. The pair-end reads were imported into CLC genomics workbench, a commercial WGS data analysis software (QIAGEN), During the imported process, the 2 fastq data files of pair end reads were merged into one files. The reads were trimmed based on the Qv values (Qv20,) and the length (reads in the length in less than 16bp were omitted). After trimming, the reads data were exported as a single fastq file. Thus we registered the fastq data as single read fastq. 287 0 Application Read Forward 1 Illumina MiSeq PRJDB3186 SAMD00078303 WGS GENOMIC RANDOM We obtained 301bp x 2 pair-end reads. The pair-end reads were imported into CLC genomics workbench, a commercial WGS data analysis software (QIAGEN), During the imported process, the 2 fastq data files of pair end reads were merged into one files. The reads were trimmed based on the Qv values (Qv20,) and the length (reads in the length in less than 16bp were omitted). After trimming, the reads data were exported as a single fastq file. Thus we registered the fastq data as single read fastq. 287 0 Application Read Forward 1 Illumina MiSeq PRJDB3186 SAMD00078304 WGS GENOMIC RANDOM We obtained 301bp x 2 pair-end reads. The pair-end reads were imported into CLC genomics workbench, a commercial WGS data analysis software (QIAGEN), During the imported process, the 2 fastq data files of pair end reads were merged into one files. The reads were trimmed based on the Qv values (Qv20,) and the length (reads in the length in less than 16bp were omitted). After trimming, the reads data were exported as a single fastq file. Thus we registered the fastq data as single read fastq. 287 0 Application Read Forward 1 Illumina MiSeq PRJDB3186 SAMD00078305 WGS GENOMIC RANDOM We obtained 301bp x 2 pair-end reads. The pair-end reads were imported into CLC genomics workbench, a commercial WGS data analysis software (QIAGEN), During the imported process, the 2 fastq data files of pair end reads were merged into one files. The reads were trimmed based on the Qv values (Qv20,) and the length (reads in the length in less than 16bp were omitted). After trimming, the reads data were exported as a single fastq file. Thus we registered the fastq data as single read fastq. 287 0 Application Read Forward 1 Illumina MiSeq PRJDB3186 SAMD00078306 WGS GENOMIC RANDOM We obtained 301bp x 2 pair-end reads. The pair-end reads were imported into CLC genomics workbench, a commercial WGS data analysis software (QIAGEN), During the imported process, the 2 fastq data files of pair end reads were merged into one files. The reads were trimmed based on the Qv values (Qv20,) and the length (reads in the length in less than 16bp were omitted). After trimming, the reads data were exported as a single fastq file. Thus we registered the fastq data as single read fastq. 287 0 Application Read Forward 1 Illumina MiSeq PRJDB3186 SAMD00078307 WGS GENOMIC RANDOM We obtained 301bp x 2 pair-end reads. The pair-end reads were imported into CLC genomics workbench, a commercial WGS data analysis software (QIAGEN), During the imported process, the 2 fastq data files of pair end reads were merged into one files. The reads were trimmed based on the Qv values (Qv20,) and the length (reads in the length in less than 16bp were omitted). After trimming, the reads data were exported as a single fastq file. Thus we registered the fastq data as single read fastq. 287 0 Application Read Forward 1 Illumina MiSeq PRJDB3186 SAMD00078308 WGS GENOMIC RANDOM We obtained 301bp x 2 pair-end reads. The pair-end reads were imported into CLC genomics workbench, a commercial WGS data analysis software (QIAGEN), During the imported process, the 2 fastq data files of pair end reads were merged into one files. The reads were trimmed based on the Qv values (Qv20,) and the length (reads in the length in less than 16bp were omitted). After trimming, the reads data were exported as a single fastq file. Thus we registered the fastq data as single read fastq. 287 0 Application Read Forward 1 Illumina MiSeq PRJDB3186 SAMD00078309 WGS GENOMIC RANDOM We obtained 301bp x 2 pair-end reads. The pair-end reads were imported into CLC genomics workbench, a commercial WGS data analysis software (QIAGEN), During the imported process, the 2 fastq data files of pair end reads were merged into one files. The reads were trimmed based on the Qv values (Qv20,) and the length (reads in the length in less than 16bp were omitted). After trimming, the reads data were exported as a single fastq file. Thus we registered the fastq data as single read fastq. 287 0 Application Read Forward 1 Illumina MiSeq PRJDB3186 SAMD00078310 WGS GENOMIC RANDOM We obtained 301bp x 2 pair-end reads. The pair-end reads were imported into CLC genomics workbench, a commercial WGS data analysis software (QIAGEN), During the imported process, the 2 fastq data files of pair end reads were merged into one files. The reads were trimmed based on the Qv values (Qv20,) and the length (reads in the length in less than 16bp were omitted). After trimming, the reads data were exported as a single fastq file. Thus we registered the fastq data as single read fastq. 287 0 Application Read Forward 1 Illumina MiSeq