PRJDB7993 The purpose of this experiment is analyzing functions of lncRNAs in the FANTOM6 project. SAMD00215736 RDhi10530_CTTGTA RNA-Seq TRANSCRIPTOMIC Inverse rRNA The libraries are prepared in accordance with the manufacturer's protocol using Illumina TruSeq Stranded Total RNA Library Prep Kit. The first step involves the removal of ribosomal RNA (rRNA) from total RNA using biotinylated, target-specific oligos combined with Ribo-Zero rRNA removal beads. The Ribo-Zero Human/Mouse/Rat kit depletes samples of cytoplasmic rRNA. Following purification, the RNA is fragmented into small pieces using divalent cations under elevated temperature. The cleaved RNA fragments are copied into first strand cDNA using reverse transcriptase and random primers, followed by second strand cDNA synthesis using DNA Polymerase I and RNase H. These cDNA fragments then have the addition of a single 'A' base and subsequent ligation of the adapter. The products are purified and enriched with PCR to create the final cDNA library. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 1 NIL PRJDB7993 The purpose of this experiment is analyzing functions of lncRNAs in the FANTOM6 project. SAMD00215735 RDhi10530_GCCAAT RNA-Seq TRANSCRIPTOMIC Inverse rRNA The libraries are prepared in accordance with the manufacturer's protocol using Illumina TruSeq Stranded Total RNA Library Prep Kit. The first step involves the removal of ribosomal RNA (rRNA) from total RNA using biotinylated, target-specific oligos combined with Ribo-Zero rRNA removal beads. The Ribo-Zero Human/Mouse/Rat kit depletes samples of cytoplasmic rRNA. Following purification, the RNA is fragmented into small pieces using divalent cations under elevated temperature. The cleaved RNA fragments are copied into first strand cDNA using reverse transcriptase and random primers, followed by second strand cDNA synthesis using DNA Polymerase I and RNase H. These cDNA fragments then have the addition of a single 'A' base and subsequent ligation of the adapter. The products are purified and enriched with PCR to create the final cDNA library. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 1 NIL PRJDB7993 The purpose of this experiment is analyzing functions of lncRNAs in the FANTOM6 project. SAMD00215759 RDhi10531_ACAGTG RNA-Seq TRANSCRIPTOMIC Inverse rRNA The libraries are prepared in accordance with the manufacturer's protocol using Illumina TruSeq Stranded Total RNA Library Prep Kit. The first step involves the removal of ribosomal RNA (rRNA) from total RNA using biotinylated, target-specific oligos combined with Ribo-Zero rRNA removal beads. The Ribo-Zero Human/Mouse/Rat kit depletes samples of cytoplasmic rRNA. Following purification, the RNA is fragmented into small pieces using divalent cations under elevated temperature. The cleaved RNA fragments are copied into first strand cDNA using reverse transcriptase and random primers, followed by second strand cDNA synthesis using DNA Polymerase I and RNase H. These cDNA fragments then have the addition of a single 'A' base and subsequent ligation of the adapter. The products are purified and enriched with PCR to create the final cDNA library. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 1 NIL PRJDB7993 The purpose of this experiment is analyzing functions of lncRNAs in the FANTOM6 project. SAMD00215760 RDhi10531_GTGAAA RNA-Seq TRANSCRIPTOMIC Inverse rRNA The libraries are prepared in accordance with the manufacturer's protocol using Illumina TruSeq Stranded Total RNA Library Prep Kit. The first step involves the removal of ribosomal RNA (rRNA) from total RNA using biotinylated, target-specific oligos combined with Ribo-Zero rRNA removal beads. The Ribo-Zero Human/Mouse/Rat kit depletes samples of cytoplasmic rRNA. Following purification, the RNA is fragmented into small pieces using divalent cations under elevated temperature. The cleaved RNA fragments are copied into first strand cDNA using reverse transcriptase and random primers, followed by second strand cDNA synthesis using DNA Polymerase I and RNase H. These cDNA fragments then have the addition of a single 'A' base and subsequent ligation of the adapter. The products are purified and enriched with PCR to create the final cDNA library. 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000 1 NIL