PRJDB12770 SAMD00436841 HEC1B_nc_r1 RNA-Seq TRANSCRIPTOMIC cDNA_oligo_dT "Magnetic beads with Oligo (dT) were used to isolate mRNA. After mixing with the fragmentation buffer, the mRNA was fragmented into short fragments. Then cDNA was synthesized using the mRNA fragments as templates. Short DNA fragments were purified and resolved with elution buffer for end reparation and single nucleotide A (adenine) addition. Subsequently, the short DNA fragments were ligated with adapters. The suitable fragments are selected for the PCR amplification as templates. During the QC steps, Agilent 2100 Bioanalyzer and ABI StepOnePlus Real-Time PCR System were used in the quantification and qualification of the sample library." 100 0 Application Read Forward 1 1 Application Read Reverse 51 BGISEQ-500 PRJDB12770 SAMD00436842 HEC1B_nc_r2 RNA-Seq TRANSCRIPTOMIC cDNA_oligo_dT "Magnetic beads with Oligo (dT) were used to isolate mRNA. After mixing with the fragmentation buffer, the mRNA was fragmented into short fragments. Then cDNA was synthesized using the mRNA fragments as templates. Short DNA fragments were purified and resolved with elution buffer for end reparation and single nucleotide A (adenine) addition. Subsequently, the short DNA fragments were ligated with adapters. The suitable fragments are selected for the PCR amplification as templates. During the QC steps, Agilent 2100 Bioanalyzer and ABI StepOnePlus Real-Time PCR System were used in the quantification and qualification of the sample library." 100 0 Application Read Forward 1 1 Application Read Reverse 51 BGISEQ-500 PRJDB12770 SAMD00436843 HEC1B_nc_r3 RNA-Seq TRANSCRIPTOMIC cDNA_oligo_dT "Magnetic beads with Oligo (dT) were used to isolate mRNA. After mixing with the fragmentation buffer, the mRNA was fragmented into short fragments. Then cDNA was synthesized using the mRNA fragments as templates. Short DNA fragments were purified and resolved with elution buffer for end reparation and single nucleotide A (adenine) addition. Subsequently, the short DNA fragments were ligated with adapters. The suitable fragments are selected for the PCR amplification as templates. During the QC steps, Agilent 2100 Bioanalyzer and ABI StepOnePlus Real-Time PCR System were used in the quantification and qualification of the sample library." 100 0 Application Read Forward 1 1 Application Read Reverse 51 BGISEQ-500 PRJDB12770 SAMD00436844 HEC1B_SETD8KD_r1 RNA-Seq TRANSCRIPTOMIC cDNA_oligo_dT "Magnetic beads with Oligo (dT) were used to isolate mRNA. After mixing with the fragmentation buffer, the mRNA was fragmented into short fragments. Then cDNA was synthesized using the mRNA fragments as templates. Short DNA fragments were purified and resolved with elution buffer for end reparation and single nucleotide A (adenine) addition. Subsequently, the short DNA fragments were ligated with adapters. The suitable fragments are selected for the PCR amplification as templates. During the QC steps, Agilent 2100 Bioanalyzer and ABI StepOnePlus Real-Time PCR System were used in the quantification and qualification of the sample library." 100 0 Application Read Forward 1 1 Application Read Reverse 51 BGISEQ-500 PRJDB12770 SAMD00436845 HEC1B_SETD8KD_r2 RNA-Seq TRANSCRIPTOMIC cDNA_oligo_dT "Magnetic beads with Oligo (dT) were used to isolate mRNA. After mixing with the fragmentation buffer, the mRNA was fragmented into short fragments. Then cDNA was synthesized using the mRNA fragments as templates. Short DNA fragments were purified and resolved with elution buffer for end reparation and single nucleotide A (adenine) addition. Subsequently, the short DNA fragments were ligated with adapters. The suitable fragments are selected for the PCR amplification as templates. During the QC steps, Agilent 2100 Bioanalyzer and ABI StepOnePlus Real-Time PCR System were used in the quantification and qualification of the sample library." 100 0 Application Read Forward 1 1 Application Read Reverse 51 BGISEQ-500 PRJDB12770 SAMD00436846 HEC1B_SETD8KD_r3 RNA-Seq TRANSCRIPTOMIC cDNA_oligo_dT "Magnetic beads with Oligo (dT) were used to isolate mRNA. After mixing with the fragmentation buffer, the mRNA was fragmented into short fragments. Then cDNA was synthesized using the mRNA fragments as templates. Short DNA fragments were purified and resolved with elution buffer for end reparation and single nucleotide A (adenine) addition. Subsequently, the short DNA fragments were ligated with adapters. The suitable fragments are selected for the PCR amplification as templates. During the QC steps, Agilent 2100 Bioanalyzer and ABI StepOnePlus Real-Time PCR System were used in the quantification and qualification of the sample library." 100 0 Application Read Forward 1 1 Application Read Reverse 51 BGISEQ-500 PRJDB12770 SAMD00436847 HEC1B_SETD8KD_r4 RNA-Seq TRANSCRIPTOMIC cDNA_oligo_dT "Magnetic beads with Oligo (dT) were used to isolate mRNA. After mixing with the fragmentation buffer, the mRNA was fragmented into short fragments. Then cDNA was synthesized using the mRNA fragments as templates. Short DNA fragments were purified and resolved with elution buffer for end reparation and single nucleotide A (adenine) addition. Subsequently, the short DNA fragments were ligated with adapters. The suitable fragments are selected for the PCR amplification as templates. During the QC steps, Agilent 2100 Bioanalyzer and ABI StepOnePlus Real-Time PCR System were used in the quantification and qualification of the sample library." 100 0 Application Read Forward 1 1 Application Read Reverse 51 BGISEQ-500 PRJDB12770 SAMD00436848 HEC50B_nc_r1 RNA-Seq TRANSCRIPTOMIC cDNA_oligo_dT "Magnetic beads with Oligo (dT) were used to isolate mRNA. After mixing with the fragmentation buffer, the mRNA was fragmented into short fragments. Then cDNA was synthesized using the mRNA fragments as templates. Short DNA fragments were purified and resolved with elution buffer for end reparation and single nucleotide A (adenine) addition. Subsequently, the short DNA fragments were ligated with adapters. The suitable fragments are selected for the PCR amplification as templates. During the QC steps, Agilent 2100 Bioanalyzer and ABI StepOnePlus Real-Time PCR System were used in the quantification and qualification of the sample library." 100 0 Application Read Forward 1 1 Application Read Reverse 51 BGISEQ-500 PRJDB12770 SAMD00436849 HEC50B_nc_r2 RNA-Seq TRANSCRIPTOMIC cDNA_oligo_dT "Magnetic beads with Oligo (dT) were used to isolate mRNA. After mixing with the fragmentation buffer, the mRNA was fragmented into short fragments. Then cDNA was synthesized using the mRNA fragments as templates. Short DNA fragments were purified and resolved with elution buffer for end reparation and single nucleotide A (adenine) addition. Subsequently, the short DNA fragments were ligated with adapters. The suitable fragments are selected for the PCR amplification as templates. During the QC steps, Agilent 2100 Bioanalyzer and ABI StepOnePlus Real-Time PCR System were used in the quantification and qualification of the sample library." 100 0 Application Read Forward 1 1 Application Read Reverse 51 BGISEQ-500 PRJDB12770 SAMD00436850 HEC50B_nc_r3 RNA-Seq TRANSCRIPTOMIC cDNA_oligo_dT "Magnetic beads with Oligo (dT) were used to isolate mRNA. After mixing with the fragmentation buffer, the mRNA was fragmented into short fragments. Then cDNA was synthesized using the mRNA fragments as templates. Short DNA fragments were purified and resolved with elution buffer for end reparation and single nucleotide A (adenine) addition. Subsequently, the short DNA fragments were ligated with adapters. The suitable fragments are selected for the PCR amplification as templates. During the QC steps, Agilent 2100 Bioanalyzer and ABI StepOnePlus Real-Time PCR System were used in the quantification and qualification of the sample library." 100 0 Application Read Forward 1 1 Application Read Reverse 51 BGISEQ-500 PRJDB12770 SAMD00436851 HEC50B_SETD8KD_r1 RNA-Seq TRANSCRIPTOMIC cDNA_oligo_dT "Magnetic beads with Oligo (dT) were used to isolate mRNA. After mixing with the fragmentation buffer, the mRNA was fragmented into short fragments. Then cDNA was synthesized using the mRNA fragments as templates. Short DNA fragments were purified and resolved with elution buffer for end reparation and single nucleotide A (adenine) addition. Subsequently, the short DNA fragments were ligated with adapters. The suitable fragments are selected for the PCR amplification as templates. During the QC steps, Agilent 2100 Bioanalyzer and ABI StepOnePlus Real-Time PCR System were used in the quantification and qualification of the sample library." 100 0 Application Read Forward 1 1 Application Read Reverse 51 BGISEQ-500 PRJDB12770 SAMD00436852 HEC50B_SETD8KD_r2 RNA-Seq TRANSCRIPTOMIC cDNA_oligo_dT "Magnetic beads with Oligo (dT) were used to isolate mRNA. After mixing with the fragmentation buffer, the mRNA was fragmented into short fragments. Then cDNA was synthesized using the mRNA fragments as templates. Short DNA fragments were purified and resolved with elution buffer for end reparation and single nucleotide A (adenine) addition. Subsequently, the short DNA fragments were ligated with adapters. The suitable fragments are selected for the PCR amplification as templates. During the QC steps, Agilent 2100 Bioanalyzer and ABI StepOnePlus Real-Time PCR System were used in the quantification and qualification of the sample library." 100 0 Application Read Forward 1 1 Application Read Reverse 51 BGISEQ-500 PRJDB12770 SAMD00436853 HEC50B_SETD8KD_r3 RNA-Seq TRANSCRIPTOMIC cDNA_oligo_dT "Magnetic beads with Oligo (dT) were used to isolate mRNA. After mixing with the fragmentation buffer, the mRNA was fragmented into short fragments. Then cDNA was synthesized using the mRNA fragments as templates. Short DNA fragments were purified and resolved with elution buffer for end reparation and single nucleotide A (adenine) addition. Subsequently, the short DNA fragments were ligated with adapters. The suitable fragments are selected for the PCR amplification as templates. During the QC steps, Agilent 2100 Bioanalyzer and ABI StepOnePlus Real-Time PCR System were used in the quantification and qualification of the sample library." 100 0 Application Read Forward 1 1 Application Read Reverse 51 BGISEQ-500 PRJDB12770 SAMD00436854 HEC50B_SETD8KD_r4 RNA-Seq TRANSCRIPTOMIC cDNA_oligo_dT "Magnetic beads with Oligo (dT) were used to isolate mRNA. After mixing with the fragmentation buffer, the mRNA was fragmented into short fragments. Then cDNA was synthesized using the mRNA fragments as templates. Short DNA fragments were purified and resolved with elution buffer for end reparation and single nucleotide A (adenine) addition. Subsequently, the short DNA fragments were ligated with adapters. The suitable fragments are selected for the PCR amplification as templates. During the QC steps, Agilent 2100 Bioanalyzer and ABI StepOnePlus Real-Time PCR System were used in the quantification and qualification of the sample library." 100 0 Application Read Forward 1 1 Application Read Reverse 51 BGISEQ-500 PRJDB12770 SAMD00436855 HEC50B_ChIP_input ChIP-Seq GENOMIC ChIP "QIAseq Ultralow Input Library Kit (QIAGEN, #180492)" 36 0 Application Read Forward 1 Illumina HiSeq 3000 PRJDB12770 SAMD00436856 HEC50B_nc_ChIP_H4K20me1 ChIP-Seq GENOMIC ChIP "QIAseq Ultralow Input Library Kit (QIAGEN, #180492)" 36 0 Application Read Forward 1 Illumina HiSeq 3000 PRJDB12770 SAMD00436857 HEC50B_KD1_ChIP_H4K20me1 ChIP-Seq GENOMIC ChIP "QIAseq Ultralow Input Library Kit (QIAGEN, #180492)" 36 0 Application Read Forward 1 Illumina HiSeq 3000 PRJDB12770 SAMD00436858 HEC50B_KD3_ChIP_H4K20me1 ChIP-Seq GENOMIC ChIP "QIAseq Ultralow Input Library Kit (QIAGEN, #180492)" 36 0 Application Read Forward 1 Illumina HiSeq 3000