PRJDB13367 Identification of species-specific single nucleotide differences in bitterlings Transcriptomes of two closely related bitterling fishes Tanakia lanceolata and T. limbata were sequenced to identify species-specific single nucleotide differences between these species. Nuclear single nucleotide genetic markers to distinguish among T. lanceolata, T. limbata and their hybrids were developed within the protein coding regions of their brain transcriptome sequences. Identification of species-specific single nucleotide differences in bitterlings bioproject PRJDB13367 PRJDB13367 true Transcriptomes of two closely related bitterling fishes Tanakia lanceolata and T. limbata were sequenced to identify species-specific single nucleotide differences between these species. Nuclear single nucleotide genetic markers to distinguish among T. lanceolata, T. limbata and their hybrids were developed within the protein coding regions of their brain transcriptome sequences.