ERX009729 SC_EXP_5187_7 <STUDY_REF accession="ERP000117" refname="Single Bacterial Cell Whole Genome Amplification-sc-2010-03-22" refcenter="SC"> <IDENTIFIERS> <PRIMARY_ID>ERP000117</PRIMARY_ID> <SUBMITTER_ID namespace="SC">Single Bacterial Cell Whole Genome Amplification-sc-2010-03-22</SUBMITTER_ID> </IDENTIFIERS> </STUDY_REF> <DESIGN> <DESIGN_DESCRIPTION>Illumina sequencing of barcoded sample library "WGAcomparison_2b" for the "Single Bacterial Cell Whole Genome Amplification" study.</DESIGN_DESCRIPTION> <SAMPLE_DESCRIPTOR accession="SRS024887"> <POOL> <DEFAULT_MEMBER> <IDENTIFIERS> <PRIMARY_ID>SRS024887</PRIMARY_ID> </IDENTIFIERS> </DEFAULT_MEMBER> <MEMBER accession="ERS013321" member_name="5187_7#7"> <IDENTIFIERS> <PRIMARY_ID>ERS013321</PRIMARY_ID> </IDENTIFIERS> <READ_LABEL read_group_tag="7">barcode_tag</READ_LABEL> </MEMBER> <MEMBER accession="ERS013322" member_name="5187_7#8"> <IDENTIFIERS> <PRIMARY_ID>ERS013322</PRIMARY_ID> </IDENTIFIERS> <READ_LABEL 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<LIBRARY_SELECTION>RANDOM</LIBRARY_SELECTION> <LIBRARY_LAYOUT> <PAIRED NOMINAL_LENGTH="287" NOMINAL_SDEV="89"/> </LIBRARY_LAYOUT> <LIBRARY_CONSTRUCTION_PROTOCOL>Standard</LIBRARY_CONSTRUCTION_PROTOCOL> </LIBRARY_DESCRIPTOR> <SPOT_DESCRIPTOR> <SPOT_DECODE_SPEC> <READ_SPEC> <READ_INDEX>0</READ_INDEX> <READ_CLASS>Application Read</READ_CLASS> <READ_TYPE>Forward</READ_TYPE> <BASE_COORD>1</BASE_COORD> </READ_SPEC> <READ_SPEC> <READ_INDEX>1</READ_INDEX> <READ_LABEL>barcode_tag</READ_LABEL> <READ_CLASS>Technical Read</READ_CLASS> <READ_TYPE>BarCode</READ_TYPE> <EXPECTED_BASECALL_TABLE> <BASECALL read_group_tag="7" min_match="7" max_mismatch="1" match_edge="end">CAGATCTG</BASECALL> <BASECALL read_group_tag="8" min_match="7" max_mismatch="1" match_edge="end">ACTTGATG</BASECALL> <BASECALL read_group_tag="9" min_match="7" max_mismatch="1" match_edge="end">GATCAGCG</BASECALL> <BASECALL read_group_tag="10" min_match="7" max_mismatch="1" match_edge="end">TAGCTTGT</BASECALL> <BASECALL read_group_tag="11" min_match="7" max_mismatch="1" match_edge="end">GGCTACAG</BASECALL> <BASECALL read_group_tag="12" min_match="7" max_mismatch="1" match_edge="end">CTTGTACT</BASECALL> </EXPECTED_BASECALL_TABLE> </READ_SPEC> <READ_SPEC> <READ_INDEX>2</READ_INDEX> <READ_CLASS>Application Read</READ_CLASS> <READ_TYPE>Reverse</READ_TYPE> <BASE_COORD>85</BASE_COORD> </READ_SPEC> </SPOT_DECODE_SPEC> </SPOT_DESCRIPTOR> </DESIGN> <PLATFORM> <ILLUMINA> <INSTRUMENT_MODEL>Illumina Genome Analyzer II</INSTRUMENT_MODEL> </ILLUMINA> </PLATFORM> <PROCESSING/> </EXPERIMENT> <EXPERIMENT alias="SC_EXP_5187_6" center_name="SC" accession="ERX009730"> <IDENTIFIERS> <PRIMARY_ID>ERX009730</PRIMARY_ID> <SUBMITTER_ID namespace="SC">SC_EXP_5187_6</SUBMITTER_ID> </IDENTIFIERS> <TITLE/> <STUDY_REF accession="ERP000117" refname="Single Bacterial Cell Whole Genome Amplification-sc-2010-03-22" refcenter="SC"> <IDENTIFIERS> <PRIMARY_ID>ERP000117</PRIMARY_ID> <SUBMITTER_ID namespace="SC">Single Bacterial Cell Whole Genome Amplification-sc-2010-03-22</SUBMITTER_ID> </IDENTIFIERS> </STUDY_REF> <DESIGN> <DESIGN_DESCRIPTION>Illumina sequencing of barcoded sample library "WGAcomparison_2a" for the "Single Bacterial Cell Whole Genome Amplification" study.</DESIGN_DESCRIPTION> <SAMPLE_DESCRIPTOR accession="SRS024887"> <POOL> <DEFAULT_MEMBER> <IDENTIFIERS> <PRIMARY_ID>SRS024887</PRIMARY_ID> </IDENTIFIERS> </DEFAULT_MEMBER> <MEMBER accession="ERS013315" member_name="5187_6#1"> <IDENTIFIERS> <PRIMARY_ID>ERS013315</PRIMARY_ID> </IDENTIFIERS> <READ_LABEL read_group_tag="1">barcode_tag</READ_LABEL> </MEMBER> <MEMBER accession="ERS013316" member_name="5187_6#2"> <IDENTIFIERS> <PRIMARY_ID>ERS013316</PRIMARY_ID> </IDENTIFIERS> <READ_LABEL read_group_tag="2">barcode_tag</READ_LABEL> </MEMBER> <MEMBER accession="ERS013317" member_name="5187_6#3"> <IDENTIFIERS> <PRIMARY_ID>ERS013317</PRIMARY_ID> </IDENTIFIERS> <READ_LABEL read_group_tag="3">barcode_tag</READ_LABEL> </MEMBER> <MEMBER accession="ERS013318" member_name="5187_6#4"> <IDENTIFIERS> <PRIMARY_ID>ERS013318</PRIMARY_ID> </IDENTIFIERS> <READ_LABEL read_group_tag="4">barcode_tag</READ_LABEL> </MEMBER> <MEMBER accession="ERS013319" member_name="5187_6#5"> <IDENTIFIERS> <PRIMARY_ID>ERS013319</PRIMARY_ID> </IDENTIFIERS> <READ_LABEL read_group_tag="5">barcode_tag</READ_LABEL> </MEMBER> <MEMBER accession="ERS013320" member_name="5187_6#6"> <IDENTIFIERS> <PRIMARY_ID>ERS013320</PRIMARY_ID> </IDENTIFIERS> <READ_LABEL read_group_tag="6">barcode_tag</READ_LABEL> </MEMBER> </POOL> </SAMPLE_DESCRIPTOR> <LIBRARY_DESCRIPTOR> <LIBRARY_NAME>WGAcomparison_2a</LIBRARY_NAME> <LIBRARY_STRATEGY>WGS</LIBRARY_STRATEGY> <LIBRARY_SOURCE>GENOMIC</LIBRARY_SOURCE> <LIBRARY_SELECTION>RANDOM</LIBRARY_SELECTION> <LIBRARY_LAYOUT> <PAIRED NOMINAL_LENGTH="301" NOMINAL_SDEV="99"/> </LIBRARY_LAYOUT> <LIBRARY_CONSTRUCTION_PROTOCOL>Standard</LIBRARY_CONSTRUCTION_PROTOCOL> </LIBRARY_DESCRIPTOR> <SPOT_DESCRIPTOR> <SPOT_DECODE_SPEC> <READ_SPEC> <READ_INDEX>0</READ_INDEX> <READ_CLASS>Application Read</READ_CLASS> <READ_TYPE>Forward</READ_TYPE> <BASE_COORD>1</BASE_COORD> </READ_SPEC> <READ_SPEC> <READ_INDEX>1</READ_INDEX> <READ_LABEL>barcode_tag</READ_LABEL> <READ_CLASS>Technical Read</READ_CLASS> <READ_TYPE>BarCode</READ_TYPE> <EXPECTED_BASECALL_TABLE> <BASECALL read_group_tag="1" min_match="6" max_mismatch="1" match_edge="end">ATCACGT</BASECALL> <BASECALL read_group_tag="2" min_match="6" max_mismatch="1" match_edge="end">CGATGTT</BASECALL> <BASECALL read_group_tag="3" min_match="6" max_mismatch="1" match_edge="end">TTAGGCA</BASECALL> <BASECALL read_group_tag="4" min_match="6" max_mismatch="1" match_edge="end">TGACCAC</BASECALL> <BASECALL read_group_tag="5" min_match="6" max_mismatch="1" match_edge="end">ACAGTGG</BASECALL> <BASECALL read_group_tag="6" min_match="6" max_mismatch="1" match_edge="end">GCCAATG</BASECALL> </EXPECTED_BASECALL_TABLE> </READ_SPEC> <READ_SPEC> <READ_INDEX>2</READ_INDEX> <READ_CLASS>Application Read</READ_CLASS> <READ_TYPE>Reverse</READ_TYPE> <BASE_COORD>85</BASE_COORD> </READ_SPEC> </SPOT_DECODE_SPEC> </SPOT_DESCRIPTOR> </DESIGN> <PLATFORM> <ILLUMINA> <INSTRUMENT_MODEL>Illumina Genome Analyzer II</INSTRUMENT_MODEL> </ILLUMINA> </PLATFORM> <PROCESSING/> </EXPERIMENT> </EXPERIMENT_SET>