ERS025256 SAMEA1027589 1427524 mixed sample Protocols: Derivatives of Pichia pastoris strain GS115 were grown to steady state in chemostat cultures (1L volume in 2L fermenter; 30oC; pH5; 750 rpm stirring; air flow 1L/min) in a defined carbon-limited medium based on sorbitol (20 g/L). Dilution rates at steady state were 0.004-0.015 L/h, and all other non-carbon nutritional requirements were in excess at constant residual concentrations. Cells were lysed mechanically in the presence of trizol using a mikrodismembranator. After partitioning with chloroform, RNA was precipitated from the aqueous phase using isopropanol. RNA was washed with 70% ethanol, redissolved in water then subjected to a lithium chloride precipitation. 10 microg aliquots of the final RNA solution were depleted for rRNA using the Ribominus Eukaryote kit for RNA seq (Invitrogen), and the rRNA-depleted sample used for sequencing library construction. ENA-FIRST-PUBLIC 2011-03-15T17:00:17Z ENA-LAST-UPDATE 2018-03-09T09:53:17Z External Id SAMEA1027589 INSDC center name EASIH INSDC first public 2011-03-15T17:00:17Z INSDC last update 2018-03-09T09:53:17Z INSDC status public StrainOrLine GS115 Submitter Id E-MTAB-562:HuLy_SS_SORB broker name ArrayExpress genotype pPIC9-HuLy sample name E-MTAB-562:HuLy_SS_SORB scientific_name Komagataella pastoris component_organism Komagataella pastoris component_organism Komagataella pastoris ERS025255 SAMEA1027592 1427524 mixed sample Protocols: Derivatives of Pichia pastoris strain GS115 were grown to steady state in chemostat cultures (1L volume in 2L fermenter; 30oC; pH5; 750 rpm stirring; air flow 1L/min) in a defined carbon-limited medium based on sorbitol (20 g/L). Dilution rates at steady state were 0.004-0.015 L/h, and all other non-carbon nutritional requirements were in excess at constant residual concentrations. Heterologous protein synthesis was induced in the strains by changing the carbon source composition of the medium to sorbitol (11.3 g/L) plus methanol (9 g/L), allowing the cultures to again reach steady state before resampling. Cells were lysed mechanically in the presence of trizol using a mikrodismembranator. After partitioning with chloroform, RNA was precipitated from the aqueous phase using isopropanol. RNA was washed with 70% ethanol, redissolved in water then subjected to a lithium chloride precipitation. 10 microg aliquots of the final RNA solution were depleted for rRNA using the Ribominus Eukaryote kit for RNA seq (Invitrogen), and the rRNA-depleted sample used for sequencing library construction. ENA-FIRST-PUBLIC 2011-03-15T17:00:17Z ENA-LAST-UPDATE 2018-03-09T09:53:17Z External Id SAMEA1027592 INSDC center name EASIH INSDC first public 2011-03-15T17:00:17Z INSDC last update 2018-03-09T09:53:17Z INSDC status public StrainOrLine GS115 Submitter Id E-MTAB-562:T70N_StM broker name ArrayExpress genotype pPIC9-HuLy_T70N sample name E-MTAB-562:T70N_StM scientific_name Komagataella pastoris component_organism Komagataella pastoris component_organism Komagataella pastoris ERS025254 SAMEA1027594 1427524 mixed sample Protocols: Derivatives of Pichia pastoris strain GS115 were grown to steady state in chemostat cultures (1L volume in 2L fermenter; 30oC; pH5; 750 rpm stirring; air flow 1L/min) in a defined carbon-limited medium based on sorbitol (20 g/L). Dilution rates at steady state were 0.004-0.015 L/h, and all other non-carbon nutritional requirements were in excess at constant residual concentrations. Heterologous protein synthesis was induced in the strains by changing the carbon source composition of the medium to sorbitol (11.3 g/L) plus methanol (9 g/L), allowing the cultures to again reach steady state before resampling. Cells were lysed mechanically in the presence of trizol using a mikrodismembranator. After partitioning with chloroform, RNA was precipitated from the aqueous phase using isopropanol. RNA was washed with 70% ethanol, redissolved in water then subjected to a lithium chloride precipitation. 10 microg aliquots of the final RNA solution were depleted for rRNA using the Ribominus Eukaryote kit for RNA seq (Invitrogen), and the rRNA-depleted sample used for sequencing library construction. ENA-FIRST-PUBLIC 2011-03-15T17:00:17Z ENA-LAST-UPDATE 2018-03-09T09:53:17Z External Id SAMEA1027594 INSDC center name EASIH INSDC first public 2011-03-15T17:00:17Z INSDC last update 2018-03-09T09:53:17Z INSDC status public StrainOrLine GS115 Submitter Id E-MTAB-562:HuLy_SS_StM broker name ArrayExpress genotype pPIC9-HuLy sample name E-MTAB-562:HuLy_SS_StM scientific_name Komagataella pastoris component_organism Komagataella pastoris component_organism Komagataella pastoris ERS025253 SAMEA1027591 1427524 mixed sample Protocols: Derivatives of Pichia pastoris strain GS115 were grown to steady state in chemostat cultures (1L volume in 2L fermenter; 30oC; pH5; 750 rpm stirring; air flow 1L/min) in a defined carbon-limited medium based on sorbitol (20 g/L). Dilution rates at steady state were 0.004-0.015 L/h, and all other non-carbon nutritional requirements were in excess at constant residual concentrations. Cells were lysed mechanically in the presence of trizol using a mikrodismembranator. After partitioning with chloroform, RNA was precipitated from the aqueous phase using isopropanol. RNA was washed with 70% ethanol, redissolved in water then subjected to a lithium chloride precipitation. 10 microg aliquots of the final RNA solution were depleted for rRNA using the Ribominus Eukaryote kit for RNA seq (Invitrogen), and the rRNA-depleted sample used for sequencing library construction. ENA-FIRST-PUBLIC 2011-03-15T17:00:17Z ENA-LAST-UPDATE 2018-03-09T09:53:17Z External Id SAMEA1027591 INSDC center name EASIH INSDC first public 2011-03-15T17:00:17Z INSDC last update 2018-03-09T09:53:17Z INSDC status public StrainOrLine GS115 Submitter Id E-MTAB-562:T70N_SORB broker name ArrayExpress genotype pPIC9-HuLy_T70N sample name E-MTAB-562:T70N_SORB scientific_name Komagataella pastoris component_organism Komagataella pastoris component_organism Komagataella pastoris ERS025252 SAMEA1027590 1427524 mixed sample Protocols: Derivatives of Pichia pastoris strain GS115 were grown to steady state in chemostat cultures (1L volume in 2L fermenter; 30oC; pH5; 750 rpm stirring; air flow 1L/min) in a defined carbon-limited medium based on sorbitol (20 g/L). Dilution rates at steady state were 0.004-0.015 L/h, and all other non-carbon nutritional requirements were in excess at constant residual concentrations. Heterologous protein synthesis was induced in the strains by changing the carbon source composition of the medium to sorbitol (11.3 g/L) plus methanol (9 g/L), allowing the cultures to again reach steady state before resampling. Cells were lysed mechanically in the presence of trizol using a mikrodismembranator. After partitioning with chloroform, RNA was precipitated from the aqueous phase using isopropanol. RNA was washed with 70% ethanol, redissolved in water then subjected to a lithium chloride precipitation. 10 microg aliquots of the final RNA solution were depleted for rRNA using the Ribominus Eukaryote kit for RNA seq (Invitrogen), and the rRNA-depleted sample used for sequencing library construction. ENA-FIRST-PUBLIC 2011-03-15T17:00:17Z ENA-LAST-UPDATE 2018-03-09T09:53:17Z External Id SAMEA1027590 INSDC center name EASIH INSDC first public 2011-03-15T17:00:17Z INSDC last update 2018-03-09T09:53:17Z INSDC status public StrainOrLine GS115 Submitter Id E-MTAB-562:PPIC9_StM broker name ArrayExpress genotype pPIC9 vector only sample name E-MTAB-562:PPIC9_StM scientific_name Komagataella pastoris component_organism Komagataella pastoris component_organism Komagataella pastoris ERS025251 SAMEA1027596 1427524 mixed sample Protocols: Derivatives of Pichia pastoris strain GS115 were grown to steady state in chemostat cultures (1L volume in 2L fermenter; 30oC; pH5; 750 rpm stirring; air flow 1L/min) in a defined carbon-limited medium based on sorbitol (20 g/L). Dilution rates at steady state were 0.004-0.015 L/h, and all other non-carbon nutritional requirements were in excess at constant residual concentrations. Heterologous protein synthesis was induced in the strains by changing the carbon source composition of the medium to sorbitol (11.3 g/L) plus methanol (9 g/L), allowing the cultures to again reach steady state before resampling. Cells were lysed mechanically in the presence of trizol using a mikrodismembranator. After partitioning with chloroform, RNA was precipitated from the aqueous phase using isopropanol. RNA was washed with 70% ethanol, redissolved in water then subjected to a lithium chloride precipitation. 10 microg aliquots of the final RNA solution were depleted for rRNA using the Ribominus Eukaryote kit for RNA seq (Invitrogen), and the rRNA-depleted sample used for sequencing library construction. ENA-FIRST-PUBLIC 2011-03-15T17:00:17Z ENA-LAST-UPDATE 2018-03-09T09:53:17Z External Id SAMEA1027596 INSDC center name EASIH INSDC first public 2011-03-15T17:00:17Z INSDC last update 2018-03-09T09:53:17Z INSDC status public StrainOrLine GS115 Submitter Id E-MTAB-562:I56T_StM broker name ArrayExpress genotype pPIC9-HuLy_I56T sample name E-MTAB-562:I56T_StM scientific_name Komagataella pastoris component_organism Komagataella pastoris component_organism Komagataella pastoris ERS025250 SAMEA1027595 1427524 mixed sample Protocols: Derivatives of Pichia pastoris strain GS115 were grown to steady state in chemostat cultures (1L volume in 2L fermenter; 30oC; pH5; 750 rpm stirring; air flow 1L/min) in a defined carbon-limited medium based on sorbitol (20 g/L). Dilution rates at steady state were 0.004-0.015 L/h, and all other non-carbon nutritional requirements were in excess at constant residual concentrations. Cells were lysed mechanically in the presence of trizol using a mikrodismembranator. After partitioning with chloroform, RNA was precipitated from the aqueous phase using isopropanol. RNA was washed with 70% ethanol, redissolved in water then subjected to a lithium chloride precipitation. 10 microg aliquots of the final RNA solution were depleted for rRNA using the Ribominus Eukaryote kit for RNA seq (Invitrogen), and the rRNA-depleted sample used for sequencing library construction. ENA-FIRST-PUBLIC 2011-03-15T17:00:17Z ENA-LAST-UPDATE 2018-03-09T09:53:17Z External Id SAMEA1027595 INSDC center name EASIH INSDC first public 2011-03-15T17:00:17Z INSDC last update 2018-03-09T09:53:17Z INSDC status public StrainOrLine GS115 Submitter Id E-MTAB-562:PPIC9_F1_SORB broker name ArrayExpress genotype pPIC9 vector only sample name E-MTAB-562:PPIC9_F1_SORB scientific_name Komagataella pastoris component_organism Komagataella pastoris component_organism Komagataella pastoris ERS025249 SAMEA1027593 1427524 mixed sample Protocols: Derivatives of Pichia pastoris strain GS115 were grown to steady state in chemostat cultures (1L volume in 2L fermenter; 30oC; pH5; 750 rpm stirring; air flow 1L/min) in a defined carbon-limited medium based on sorbitol (20 g/L). Dilution rates at steady state were 0.004-0.015 L/h, and all other non-carbon nutritional requirements were in excess at constant residual concentrations. Cells were lysed mechanically in the presence of trizol using a mikrodismembranator. After partitioning with chloroform, RNA was precipitated from the aqueous phase using isopropanol. RNA was washed with 70% ethanol, redissolved in water then subjected to a lithium chloride precipitation. 10 microg aliquots of the final RNA solution were depleted for rRNA using the Ribominus Eukaryote kit for RNA seq (Invitrogen), and the rRNA-depleted sample used for sequencing library construction. ENA-FIRST-PUBLIC 2011-03-15T17:00:17Z ENA-LAST-UPDATE 2018-03-09T09:53:17Z External Id SAMEA1027593 INSDC center name EASIH INSDC first public 2011-03-15T17:00:17Z INSDC last update 2018-03-09T09:53:17Z INSDC status public StrainOrLine GS115 Submitter Id E-MTAB-562:I56T_F1_SORB broker name ArrayExpress genotype pPIC9-HuLy_I56T sample name E-MTAB-562:I56T_F1_SORB scientific_name Komagataella pastoris component_organism Komagataella pastoris component_organism Komagataella pastoris