ERP000757 PRJEB2594 SNP_Discovery_on_the_Zebrafish_MGH_cross_-sc-2011-06-28T11:14:29Z-667 SNP_Discovery_on_the_Zebrafish_MGH_cross_ Early resequencing study to identify SNPs within NspI fragments that are polymorphic between AB and India in the large MGH zebrafish meiotic cross (Knapik et al 1996). The DNAs were processed as they would be for the Affy SNP5 platform - NspI digest, adaptor ligation and PCR. By slightly changing the adaptor sequence, it recreated an NspI site, so that the PCR products could be cleaved and sequenced with Illumina GA technology. SNP_Discovery_on_the_Zebrafish_MGH_cross_ Early resequencing study to identify SNPs within NspI fragments that are polymorphic between AB and India in the large MGH zebrafish meiotic cross (Knapik et al 1996). The DNAs were processed as they would be for the Affy SNP5 platform - NspI digest, adaptor ligation and PCR. By slightly changing the adaptor sequence (ATTATGAGCACGACAGACGCCTGATCTRCATG and YAGATCAGGCGTCTGTCGTGCTCATAA), it recreated an NspI site, so that the PCR products could be cleaved and the genomic DNA sequenced with Illumina GA technology.. This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/