ERX8902497 SSA_ROSLIN_AF_WP1_RNA_01_3 ERP136505 PRJEB51855 Stranded PE150 RNA-Seq experiment using polyA RNA selection and seqencing on a Novaseq 6000 ERS11209608 SAMEA9835754 D1300103 RNA-Seq TRANSCRIPTOMIC other NEBNext Ultra Directional RNA Library Prep Kit for Illumina E7420 manual Illumina NovaSeq 6000 project FAANG secondary project AQUA-FAANG assay type RNA-seq of coding RNA sample storage TRIzol, frozen sample storage processing fresh sampling to preparation interval 10.0 months experimental protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_RNAseq_protocol_20210527.pdf extraction protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_RNAseq_protocol_20210527.pdf library preparation location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road library preparation location longitude 0.145393 decimal degrees library preparation location latitude 52.231532 decimal degrees library preparation date 2020-10-10 YYYY-MM-DD sequencing location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road sequencing location longitude 0.145393 decimal degrees sequencing location latitude 52.231532 decimal degrees sequencing date 2020-03-13 YYYY-MM-DD experiment target rna rna preparation 3 adapter ligation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD rna preparation 5 adapter ligation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD library generation pcr product isolation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD preparation reverse transcription protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD library generation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD read strand mate 2 sense rna purity 260-230 ratio 1.39 rna integrity number 7.8 ERX8902498 SSA_ROSLIN_AF_WP1_RNA_02_1 ERP136505 PRJEB51855 Stranded PE150 RNA-Seq experiment using polyA RNA selection and seqencing on a Novaseq 6000 ERS11209589 SAMEA9835700 D1300201 RNA-Seq TRANSCRIPTOMIC other NEBNext Ultra Directional RNA Library Prep Kit for Illumina E7420 manual Illumina NovaSeq 6000 project FAANG secondary project AQUA-FAANG assay type RNA-seq of coding RNA sample storage TRIzol, frozen sample storage processing fresh sampling to preparation interval 10.0 months experimental protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_RNAseq_protocol_20210527.pdf extraction protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_RNAseq_protocol_20210527.pdf library preparation location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road library preparation location longitude 0.145393 decimal degrees library preparation location latitude 52.231532 decimal degrees library preparation date 2020-10-10 YYYY-MM-DD sequencing location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road sequencing location longitude 0.145393 decimal degrees sequencing location latitude 52.231532 decimal degrees sequencing date 2020-03-13 YYYY-MM-DD experiment target rna rna preparation 3 adapter ligation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD rna preparation 5 adapter ligation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD library generation pcr product isolation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD preparation reverse transcription protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD library generation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD read strand mate 2 sense rna purity 260-230 ratio 1.37 rna integrity number 8.8 ERX8902499 SSA_ROSLIN_AF_WP1_RNA_02_2 ERP136505 PRJEB51855 Stranded PE150 RNA-Seq experiment using polyA RNA selection and seqencing on a Novaseq 6000 ERS11209647 SAMEA9835728 D1300202 RNA-Seq TRANSCRIPTOMIC other NEBNext Ultra Directional RNA Library Prep Kit for Illumina E7420 manual Illumina NovaSeq 6000 project FAANG secondary project AQUA-FAANG assay type RNA-seq of coding RNA sample storage TRIzol, frozen sample storage processing fresh sampling to preparation interval 10.0 months experimental protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_RNAseq_protocol_20210527.pdf extraction protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_RNAseq_protocol_20210527.pdf library preparation location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road library preparation location longitude 0.145393 decimal degrees library preparation location latitude 52.231532 decimal degrees library preparation date 2020-10-10 YYYY-MM-DD sequencing location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road sequencing location longitude 0.145393 decimal degrees sequencing location latitude 52.231532 decimal degrees sequencing date 2020-03-13 YYYY-MM-DD experiment target rna rna preparation 3 adapter ligation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD rna preparation 5 adapter ligation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD library generation pcr product isolation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD preparation reverse transcription protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD library generation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD read strand mate 2 sense rna purity 260-230 ratio 1.3 rna integrity number 8.5 ERX8902500 SSA_ROSLIN_AF_WP1_RNA_02_3 ERP136505 PRJEB51855 Stranded PE150 RNA-Seq experiment using polyA RNA selection and seqencing on a Novaseq 6000 ERS11209611 SAMEA9835756 D1300203 RNA-Seq TRANSCRIPTOMIC other NEBNext Ultra Directional RNA Library Prep Kit for Illumina E7420 manual Illumina NovaSeq 6000 project FAANG secondary project AQUA-FAANG assay type RNA-seq of coding RNA sample storage TRIzol, frozen sample storage processing fresh sampling to preparation interval 10.0 months experimental protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_RNAseq_protocol_20210527.pdf extraction protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_RNAseq_protocol_20210527.pdf library preparation location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road library preparation location longitude 0.145393 decimal degrees library preparation location latitude 52.231532 decimal degrees library preparation date 2020-10-10 YYYY-MM-DD sequencing location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road sequencing location longitude 0.145393 decimal degrees sequencing location latitude 52.231532 decimal degrees sequencing date 2020-03-13 YYYY-MM-DD experiment target rna rna preparation 3 adapter ligation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD rna preparation 5 adapter ligation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD library generation pcr product isolation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD preparation reverse transcription protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD library generation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD read strand mate 2 sense rna purity 260-230 ratio 1.41 rna integrity number 8.2 ERX8902501 SSA_ROSLIN_AF_WP1_RNA_03_1 ERP136505 PRJEB51855 Stranded PE150 RNA-Seq experiment using polyA RNA selection and seqencing on a Novaseq 6000 ERS11209592 SAMEA9835702 D1300301 RNA-Seq TRANSCRIPTOMIC other NEBNext Ultra Directional RNA Library Prep Kit for Illumina E7420 manual Illumina NovaSeq 6000 project FAANG secondary project AQUA-FAANG assay type RNA-seq of coding RNA sample storage TRIzol, frozen sample storage processing fresh sampling to preparation interval 10.0 months experimental protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_RNAseq_protocol_20210527.pdf extraction protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_RNAseq_protocol_20210527.pdf library preparation location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road library preparation location longitude 0.145393 decimal degrees library preparation location latitude 52.231532 decimal degrees library preparation date 2020-10-10 YYYY-MM-DD sequencing location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road sequencing location longitude 0.145393 decimal degrees sequencing location latitude 52.231532 decimal degrees sequencing date 2020-03-13 YYYY-MM-DD experiment target rna rna preparation 3 adapter ligation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD rna preparation 5 adapter ligation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD library generation pcr product isolation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD preparation reverse transcription protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD library generation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD read strand mate 2 sense rna purity 260-230 ratio 1.89 rna integrity number 9.4 ERX8902503 SSA_ROSLIN_AF_WP1_RNA_03_3 ERP136505 PRJEB51855 Stranded PE150 RNA-Seq experiment using polyA RNA selection and seqencing on a Novaseq 6000 ERS11209613 SAMEA9835758 D1300303 RNA-Seq TRANSCRIPTOMIC other NEBNext Ultra Directional RNA Library Prep Kit for Illumina E7420 manual Illumina NovaSeq 6000 project FAANG secondary project AQUA-FAANG assay type RNA-seq of coding RNA sample storage TRIzol, frozen sample storage processing fresh sampling to preparation interval 10.0 months experimental protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_RNAseq_protocol_20210527.pdf extraction protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_RNAseq_protocol_20210527.pdf library preparation location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road library preparation location longitude 0.145393 decimal degrees library preparation location latitude 52.231532 decimal degrees library preparation date 2020-10-10 YYYY-MM-DD sequencing location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road sequencing location longitude 0.145393 decimal degrees sequencing location latitude 52.231532 decimal degrees sequencing date 2020-03-13 YYYY-MM-DD experiment target rna rna preparation 3 adapter ligation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD rna preparation 5 adapter ligation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD library generation pcr product isolation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD preparation reverse transcription protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD library generation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD read strand mate 2 sense rna purity 260-230 ratio 1.77 rna integrity number 9.7 ERX8902504 SSA_ROSLIN_AF_WP1_RNA_04_1 ERP136505 PRJEB51855 Stranded PE150 RNA-Seq experiment using polyA RNA selection and seqencing on a Novaseq 6000 ERS11209595 SAMEA9835704 D1300401 RNA-Seq TRANSCRIPTOMIC other NEBNext Ultra Directional RNA Library Prep Kit for Illumina E7420 manual Illumina NovaSeq 6000 project FAANG secondary project AQUA-FAANG assay type RNA-seq of coding RNA sample storage TRIzol, frozen sample storage processing fresh sampling to preparation interval 10.0 months experimental protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_RNAseq_protocol_20210527.pdf extraction protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_RNAseq_protocol_20210527.pdf library preparation location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road library preparation location longitude 0.145393 decimal degrees library preparation location latitude 52.231532 decimal degrees library preparation date 2020-10-10 YYYY-MM-DD sequencing location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road sequencing location longitude 0.145393 decimal degrees sequencing location latitude 52.231532 decimal degrees sequencing date 2020-03-13 YYYY-MM-DD experiment target rna rna preparation 3 adapter ligation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD rna preparation 5 adapter ligation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD library generation pcr product isolation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD preparation reverse transcription protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD library generation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD read strand mate 2 sense rna purity 260-230 ratio 1.8 rna integrity number 9.4 ERX8902506 SSA_ROSLIN_AF_WP1_RNA_04_3 ERP136505 PRJEB51855 Stranded PE150 RNA-Seq experiment using polyA RNA selection and seqencing on a Novaseq 6000 ERS11209631 SAMEA9835760 D1300403 RNA-Seq TRANSCRIPTOMIC other NEBNext Ultra Directional RNA Library Prep Kit for Illumina E7420 manual Illumina NovaSeq 6000 project FAANG secondary project AQUA-FAANG assay type RNA-seq of coding RNA sample storage TRIzol, frozen sample storage processing fresh sampling to preparation interval 10.0 months experimental protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_RNAseq_protocol_20210527.pdf extraction protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_RNAseq_protocol_20210527.pdf library preparation location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road library preparation location longitude 0.145393 decimal degrees library preparation location latitude 52.231532 decimal degrees library preparation date 2020-10-10 YYYY-MM-DD sequencing location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road sequencing location longitude 0.145393 decimal degrees sequencing location latitude 52.231532 decimal degrees sequencing date 2020-03-13 YYYY-MM-DD experiment target rna rna preparation 3 adapter ligation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD rna preparation 5 adapter ligation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD library generation pcr product isolation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD preparation reverse transcription protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD library generation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD read strand mate 2 sense rna purity 260-230 ratio 1.82 rna integrity number 8.4 ERX8902507 SSA_ROSLIN_AF_WP1_RNA_05_1 ERP136505 PRJEB51855 Stranded PE150 RNA-Seq experiment using polyA RNA selection and seqencing on a Novaseq 6000 ERS11209621 SAMEA9835706 D1300501 RNA-Seq TRANSCRIPTOMIC other NEBNext Ultra Directional RNA Library Prep Kit for Illumina E7420 manual Illumina NovaSeq 6000 project FAANG secondary project AQUA-FAANG assay type RNA-seq of coding RNA sample storage TRIzol, frozen sample storage processing fresh sampling to preparation interval 10.0 months experimental protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_RNAseq_protocol_20210527.pdf extraction protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_RNAseq_protocol_20210527.pdf library preparation location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road library preparation location longitude 0.145393 decimal degrees library preparation location latitude 52.231532 decimal degrees library preparation date 2020-10-10 YYYY-MM-DD sequencing location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road sequencing location longitude 0.145393 decimal degrees sequencing location latitude 52.231532 decimal degrees sequencing date 2020-03-13 YYYY-MM-DD experiment target rna rna preparation 3 adapter ligation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD rna preparation 5 adapter ligation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD library generation pcr product isolation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD preparation reverse transcription protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD library generation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD read strand mate 2 sense rna purity 260-230 ratio 2.33 rna integrity number 9.8 ERX8902509 SSA_ROSLIN_AF_WP1_RNA_05_3 ERP136505 PRJEB51855 Stranded PE150 RNA-Seq experiment using polyA RNA selection and seqencing on a Novaseq 6000 ERS11209632 SAMEA9835762 D1300503 RNA-Seq TRANSCRIPTOMIC other NEBNext Ultra Directional RNA Library Prep Kit for Illumina E7420 manual Illumina NovaSeq 6000 project FAANG secondary project AQUA-FAANG assay type RNA-seq of coding RNA sample storage TRIzol, frozen sample storage processing fresh sampling to preparation interval 10.0 months experimental protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_RNAseq_protocol_20210527.pdf extraction protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_RNAseq_protocol_20210527.pdf library preparation location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road library preparation location longitude 0.145393 decimal degrees library preparation location latitude 52.231532 decimal degrees library preparation date 2020-10-10 YYYY-MM-DD sequencing location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road sequencing location longitude 0.145393 decimal degrees sequencing location latitude 52.231532 decimal degrees sequencing date 2020-03-13 YYYY-MM-DD experiment target rna rna preparation 3 adapter ligation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD rna preparation 5 adapter ligation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD library generation pcr product isolation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD preparation reverse transcription protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD library generation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD read strand mate 2 sense rna purity 260-230 ratio 2.21 rna integrity number 8.4 ERX8902510 SSA_ROSLIN_AF_WP1_RNA_06_1 ERP136505 PRJEB51855 Stranded PE150 RNA-Seq experiment using polyA RNA selection and seqencing on a Novaseq 6000 ERS11209623 SAMEA9835708 D1300601 RNA-Seq TRANSCRIPTOMIC other NEBNext Ultra Directional RNA Library Prep Kit for Illumina E7420 manual Illumina NovaSeq 6000 project FAANG secondary project AQUA-FAANG assay type RNA-seq of coding RNA sample storage TRIzol, frozen sample storage processing fresh sampling to preparation interval 10.0 months experimental protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_RNAseq_protocol_20210527.pdf extraction protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_RNAseq_protocol_20210527.pdf library preparation location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road library preparation location longitude 0.145393 decimal degrees library preparation location latitude 52.231532 decimal degrees library preparation date 2020-10-10 YYYY-MM-DD sequencing location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road sequencing location longitude 0.145393 decimal degrees sequencing location latitude 52.231532 decimal degrees sequencing date 2020-03-13 YYYY-MM-DD experiment target rna rna preparation 3 adapter ligation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD rna preparation 5 adapter ligation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD library generation pcr product isolation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD preparation reverse transcription protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD library generation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD read strand mate 2 sense rna purity 260-230 ratio 2.29 rna integrity number 7.6 ERX8902512 SSA_ROSLIN_AF_WP1_RNA_06_3 ERP136505 PRJEB51855 Stranded PE150 RNA-Seq experiment using polyA RNA selection and seqencing on a Novaseq 6000 ERS11209625 SAMEA9835764 D1300603 RNA-Seq TRANSCRIPTOMIC other NEBNext Ultra Directional RNA Library Prep Kit for Illumina E7420 manual Illumina NovaSeq 6000 project FAANG secondary project AQUA-FAANG assay type RNA-seq of coding RNA sample storage TRIzol, frozen sample storage processing fresh sampling to preparation interval 10.0 months experimental protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_RNAseq_protocol_20210527.pdf extraction protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_RNAseq_protocol_20210527.pdf library preparation location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road library preparation location longitude 0.145393 decimal degrees library preparation location latitude 52.231532 decimal degrees library preparation date 2020-10-10 YYYY-MM-DD sequencing location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road sequencing location longitude 0.145393 decimal degrees sequencing location latitude 52.231532 decimal degrees sequencing date 2020-03-13 YYYY-MM-DD experiment target rna rna preparation 3 adapter ligation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD rna preparation 5 adapter ligation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD library generation pcr product isolation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD preparation reverse transcription protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD library generation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD read strand mate 2 sense rna purity 260-230 ratio 1.72 rna integrity number 6.6 ERX8902513 SSA_ROSLIN_AF_WP1_RNA_07_1 ERP136505 PRJEB51855 Stranded PE150 RNA-Seq experiment using polyA RNA selection and seqencing on a Novaseq 6000 ERS11209646 SAMEA9835695 D1300701 RNA-Seq TRANSCRIPTOMIC other NEBNext Ultra Directional RNA Library Prep Kit for Illumina E7420 manual Illumina NovaSeq 6000 project FAANG secondary project AQUA-FAANG assay type RNA-seq of coding RNA sample storage TRIzol, frozen sample storage processing fresh sampling to preparation interval 10.0 months experimental protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_RNAseq_protocol_20210527.pdf extraction protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_RNAseq_protocol_20210527.pdf library preparation location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road library preparation location longitude 0.145393 decimal degrees library preparation location latitude 52.231532 decimal degrees library preparation date 2020-10-10 YYYY-MM-DD sequencing location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road sequencing location longitude 0.145393 decimal degrees sequencing location latitude 52.231532 decimal degrees sequencing date 2020-03-13 YYYY-MM-DD experiment target rna rna preparation 3 adapter ligation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD rna preparation 5 adapter ligation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD library generation pcr product isolation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD preparation reverse transcription protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD library generation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD read strand mate 2 sense rna purity 260-230 ratio 1.13 ERX8902514 SSA_ROSLIN_AF_WP1_RNA_07_2 ERP136505 PRJEB51855 Stranded PE150 RNA-Seq experiment using polyA RNA selection and seqencing on a Novaseq 6000 ERS11209628 SAMEA9835723 D1300702 RNA-Seq TRANSCRIPTOMIC other NEBNext Ultra Directional RNA Library Prep Kit for Illumina E7420 manual Illumina NovaSeq 6000 project FAANG secondary project AQUA-FAANG assay type RNA-seq of coding RNA sample storage TRIzol, frozen sample storage processing fresh sampling to preparation interval 10.0 months experimental protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_RNAseq_protocol_20210527.pdf extraction protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_RNAseq_protocol_20210527.pdf library preparation location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road library preparation location longitude 0.145393 decimal degrees library preparation location latitude 52.231532 decimal degrees library preparation date 2020-10-10 YYYY-MM-DD sequencing location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road sequencing location longitude 0.145393 decimal degrees sequencing location latitude 52.231532 decimal degrees sequencing date 2020-03-13 YYYY-MM-DD experiment target rna rna preparation 3 adapter ligation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD rna preparation 5 adapter ligation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD library generation pcr product isolation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD preparation reverse transcription protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD library generation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD read strand mate 2 sense rna purity 260-230 ratio 1.01 ERX8902516 SSA_ROSLIN_AF_WP1_RNA_08_1 ERP136505 PRJEB51855 Stranded PE150 RNA-Seq experiment using polyA RNA selection and seqencing on a Novaseq 6000 ERS11209645 SAMEA9835696 D1300801 RNA-Seq TRANSCRIPTOMIC other NEBNext Ultra Directional RNA Library Prep Kit for Illumina E7420 manual Illumina NovaSeq 6000 project FAANG secondary project AQUA-FAANG assay type RNA-seq of coding RNA sample storage TRIzol, frozen sample storage processing fresh sampling to preparation interval 10.0 months experimental protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_RNAseq_protocol_20210527.pdf extraction protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_RNAseq_protocol_20210527.pdf library preparation location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road library preparation location longitude 0.145393 decimal degrees library preparation location latitude 52.231532 decimal degrees library preparation date 2020-10-10 YYYY-MM-DD sequencing location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road sequencing location longitude 0.145393 decimal degrees sequencing location latitude 52.231532 decimal degrees sequencing date 2020-03-13 YYYY-MM-DD experiment target rna rna preparation 3 adapter ligation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD rna preparation 5 adapter ligation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD library generation pcr product isolation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD preparation reverse transcription protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD library generation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD read strand mate 2 sense rna purity 260-230 ratio 1.28 rna integrity number 7.1 ERX8902517 SSA_ROSLIN_AF_WP1_RNA_08_2 ERP136505 PRJEB51855 Stranded PE150 RNA-Seq experiment using polyA RNA selection and seqencing on a Novaseq 6000 ERS11209627 SAMEA9835724 D1300802 RNA-Seq TRANSCRIPTOMIC other NEBNext Ultra Directional RNA Library Prep Kit for Illumina E7420 manual Illumina NovaSeq 6000 project FAANG secondary project AQUA-FAANG assay type RNA-seq of coding RNA sample storage TRIzol, frozen sample storage processing fresh sampling to preparation interval 10.0 months experimental protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_RNAseq_protocol_20210527.pdf extraction protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_RNAseq_protocol_20210527.pdf library preparation location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road library preparation location longitude 0.145393 decimal degrees library preparation location latitude 52.231532 decimal degrees library preparation date 2020-10-10 YYYY-MM-DD sequencing location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road sequencing location longitude 0.145393 decimal degrees sequencing location latitude 52.231532 decimal degrees sequencing date 2020-03-13 YYYY-MM-DD experiment target rna rna preparation 3 adapter ligation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD rna preparation 5 adapter ligation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD library generation pcr product isolation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD preparation reverse transcription protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD library generation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD read strand mate 2 sense rna purity 260-230 ratio 0.96 rna integrity number 8.2 ERX8902520 SSA_ROSLIN_AF_WP1_RNA_09_2 ERP136505 PRJEB51855 Stranded PE150 RNA-Seq experiment using polyA RNA selection and seqencing on a Novaseq 6000 ERS11209630 SAMEA9835725 D1300902 RNA-Seq TRANSCRIPTOMIC other NEBNext Ultra Directional RNA Library Prep Kit for Illumina E7420 manual Illumina NovaSeq 6000 project FAANG secondary project AQUA-FAANG assay type RNA-seq of coding RNA sample storage TRIzol, frozen sample storage processing fresh sampling to preparation interval 10.0 months experimental protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_RNAseq_protocol_20210527.pdf extraction protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_RNAseq_protocol_20210527.pdf library preparation location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road library preparation location longitude 0.145393 decimal degrees library preparation location latitude 52.231532 decimal degrees library preparation date 2020-10-10 YYYY-MM-DD sequencing location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road sequencing location longitude 0.145393 decimal degrees sequencing location latitude 52.231532 decimal degrees sequencing date 2020-03-13 YYYY-MM-DD experiment target rna rna preparation 3 adapter ligation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD rna preparation 5 adapter ligation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD library generation pcr product isolation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD preparation reverse transcription protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD library generation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD read strand mate 2 sense rna purity 260-230 ratio 1.58 rna integrity number 8.3 ERX8902521 SSA_ROSLIN_AF_WP1_RNA_09_3 ERP136505 PRJEB51855 Stranded PE150 RNA-Seq experiment using polyA RNA selection and seqencing on a Novaseq 6000 ERS11209602 SAMEA9835753 D1300903 RNA-Seq TRANSCRIPTOMIC other NEBNext Ultra Directional RNA Library Prep Kit for Illumina E7420 manual Illumina NovaSeq 6000 project FAANG secondary project AQUA-FAANG assay type RNA-seq of coding RNA sample storage TRIzol, frozen sample storage processing fresh sampling to preparation interval 10.0 months experimental protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_RNAseq_protocol_20210527.pdf extraction protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_RNAseq_protocol_20210527.pdf library preparation location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road library preparation location longitude 0.145393 decimal degrees library preparation location latitude 52.231532 decimal degrees library preparation date 2020-10-10 YYYY-MM-DD sequencing location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road sequencing location longitude 0.145393 decimal degrees sequencing location latitude 52.231532 decimal degrees sequencing date 2020-03-13 YYYY-MM-DD experiment target rna rna preparation 3 adapter ligation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD rna preparation 5 adapter ligation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD library generation pcr product isolation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD preparation reverse transcription protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD library generation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD read strand mate 2 sense rna purity 260-230 ratio 1.32 rna integrity number 8.5 ERX8902523 SSA_ROSLIN_AF_WP1_RNA_10_2 ERP136505 PRJEB51855 Stranded PE150 RNA-Seq experiment using polyA RNA selection and seqencing on a Novaseq 6000 ERS11209648 SAMEA9835727 D1301002 RNA-Seq TRANSCRIPTOMIC other NEBNext Ultra Directional RNA Library Prep Kit for Illumina E7420 manual Illumina NovaSeq 6000 project FAANG secondary project AQUA-FAANG assay type RNA-seq of coding RNA sample storage TRIzol, frozen sample storage processing fresh sampling to preparation interval 10.0 months experimental protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_RNAseq_protocol_20210527.pdf extraction protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_RNAseq_protocol_20210527.pdf library preparation location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road library preparation location longitude 0.145393 decimal degrees library preparation location latitude 52.231532 decimal degrees library preparation date 2020-10-10 YYYY-MM-DD sequencing location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road sequencing location longitude 0.145393 decimal degrees sequencing location latitude 52.231532 decimal degrees sequencing date 2020-03-13 YYYY-MM-DD experiment target rna rna preparation 3 adapter ligation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD rna preparation 5 adapter ligation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD library generation pcr product isolation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD preparation reverse transcription protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD library generation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD read strand mate 2 sense rna purity 260-230 ratio 1.67 rna integrity number 9.3 ERX8902524 SSA_ROSLIN_AF_WP1_RNA_10_3 ERP136505 PRJEB51855 Stranded PE150 RNA-Seq experiment using polyA RNA selection and seqencing on a Novaseq 6000 ERS11209606 SAMEA9835755 D1301003 RNA-Seq TRANSCRIPTOMIC other NEBNext Ultra Directional RNA Library Prep Kit for Illumina E7420 manual Illumina NovaSeq 6000 project FAANG secondary project AQUA-FAANG assay type RNA-seq of coding RNA sample storage TRIzol, frozen sample storage processing fresh sampling to preparation interval 10.0 months experimental protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_RNAseq_protocol_20210527.pdf extraction protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_RNAseq_protocol_20210527.pdf library preparation location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road library preparation location longitude 0.145393 decimal degrees library preparation location latitude 52.231532 decimal degrees library preparation date 2020-10-10 YYYY-MM-DD sequencing location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road sequencing location longitude 0.145393 decimal degrees sequencing location latitude 52.231532 decimal degrees sequencing date 2020-03-13 YYYY-MM-DD experiment target rna rna preparation 3 adapter ligation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD rna preparation 5 adapter ligation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD library generation pcr product isolation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD preparation reverse transcription protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD library generation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD read strand mate 2 sense rna purity 260-230 ratio 1.21 rna integrity number 8.8 ERX8902525 SSA_ROSLIN_AF_WP1_RNA_11_1 ERP136505 PRJEB51855 Stranded PE150 RNA-Seq experiment using polyA RNA selection and seqencing on a Novaseq 6000 ERS11209593 SAMEA9835701 D1301101 RNA-Seq TRANSCRIPTOMIC other NEBNext Ultra Directional RNA Library Prep Kit for Illumina E7420 manual Illumina NovaSeq 6000 project FAANG secondary project AQUA-FAANG assay type RNA-seq of coding RNA sample storage TRIzol, frozen sample storage processing fresh sampling to preparation interval 10.0 months experimental protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_RNAseq_protocol_20210527.pdf extraction protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_RNAseq_protocol_20210527.pdf library preparation location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road library preparation location longitude 0.145393 decimal degrees library preparation location latitude 52.231532 decimal degrees library preparation date 2020-10-10 YYYY-MM-DD sequencing location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road sequencing location longitude 0.145393 decimal degrees sequencing location latitude 52.231532 decimal degrees sequencing date 2020-03-13 YYYY-MM-DD experiment target rna rna preparation 3 adapter ligation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD rna preparation 5 adapter ligation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD library generation pcr product isolation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD preparation reverse transcription protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD library generation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD read strand mate 2 sense rna purity 260-230 ratio 1.63 rna integrity number 8.2 ERX8902526 SSA_ROSLIN_AF_WP1_RNA_11_2 ERP136505 PRJEB51855 Stranded PE150 RNA-Seq experiment using polyA RNA selection and seqencing on a Novaseq 6000 ERS11209649 SAMEA9835729 D1301102 RNA-Seq TRANSCRIPTOMIC other NEBNext Ultra Directional RNA Library Prep Kit for Illumina E7420 manual Illumina NovaSeq 6000 project FAANG secondary project AQUA-FAANG assay type RNA-seq of coding RNA sample storage TRIzol, frozen sample storage processing fresh sampling to preparation interval 10.0 months experimental protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_RNAseq_protocol_20210527.pdf extraction protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_RNAseq_protocol_20210527.pdf library preparation location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road library preparation location longitude 0.145393 decimal degrees library preparation location latitude 52.231532 decimal degrees library preparation date 2020-10-10 YYYY-MM-DD sequencing location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road sequencing location longitude 0.145393 decimal degrees sequencing location latitude 52.231532 decimal degrees sequencing date 2020-03-13 YYYY-MM-DD experiment target rna rna preparation 3 adapter ligation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD rna preparation 5 adapter ligation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD library generation pcr product isolation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD preparation reverse transcription protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD library generation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD read strand mate 2 sense rna purity 260-230 ratio 1.26 rna integrity number 8.3 ERX8902527 SSA_ROSLIN_AF_WP1_RNA_11_3 ERP136505 PRJEB51855 Stranded PE150 RNA-Seq experiment using polyA RNA selection and seqencing on a Novaseq 6000 ERS11209610 SAMEA9835757 D1301103 RNA-Seq TRANSCRIPTOMIC other NEBNext Ultra Directional RNA Library Prep Kit for Illumina E7420 manual Illumina NovaSeq 6000 project FAANG secondary project AQUA-FAANG assay type RNA-seq of coding RNA sample storage TRIzol, frozen sample storage processing fresh sampling to preparation interval 10.0 months experimental protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_RNAseq_protocol_20210527.pdf extraction protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_RNAseq_protocol_20210527.pdf library preparation location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road library preparation location longitude 0.145393 decimal degrees library preparation location latitude 52.231532 decimal degrees library preparation date 2020-10-10 YYYY-MM-DD sequencing location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road sequencing location longitude 0.145393 decimal degrees sequencing location latitude 52.231532 decimal degrees sequencing date 2020-03-13 YYYY-MM-DD experiment target rna rna preparation 3 adapter ligation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD rna preparation 5 adapter ligation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD library generation pcr product isolation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD preparation reverse transcription protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD library generation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD read strand mate 2 sense rna purity 260-230 ratio 1.23 rna integrity number 8.7 ERX8902528 SSA_ROSLIN_AF_WP1_RNA_12_1 ERP136505 PRJEB51855 Stranded PE150 RNA-Seq experiment using polyA RNA selection and seqencing on a Novaseq 6000 ERS11209596 SAMEA9835703 D1301201 RNA-Seq TRANSCRIPTOMIC other NEBNext Ultra Directional RNA Library Prep Kit for Illumina E7420 manual Illumina NovaSeq 6000 project FAANG secondary project AQUA-FAANG assay type RNA-seq of coding RNA sample storage TRIzol, frozen sample storage processing fresh sampling to preparation interval 10.0 months experimental protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_RNAseq_protocol_20210527.pdf extraction protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_RNAseq_protocol_20210527.pdf library preparation location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road library preparation location longitude 0.145393 decimal degrees library preparation location latitude 52.231532 decimal degrees library preparation date 2020-10-10 YYYY-MM-DD sequencing location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road sequencing location longitude 0.145393 decimal degrees sequencing location latitude 52.231532 decimal degrees sequencing date 2020-03-13 YYYY-MM-DD experiment target rna rna preparation 3 adapter ligation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD rna preparation 5 adapter ligation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD library generation pcr product isolation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD preparation reverse transcription protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD library generation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD read strand mate 2 sense rna purity 260-230 ratio 2.36 rna integrity number 9.9 ERX8902529 SSA_ROSLIN_AF_WP1_RNA_12_2 ERP136505 PRJEB51855 Stranded PE150 RNA-Seq experiment using polyA RNA selection and seqencing on a Novaseq 6000 ERS11209635 SAMEA9835731 D1301202 RNA-Seq TRANSCRIPTOMIC other NEBNext Ultra Directional RNA Library Prep Kit for Illumina E7420 manual Illumina NovaSeq 6000 project FAANG secondary project AQUA-FAANG assay type RNA-seq of coding RNA sample storage TRIzol, frozen sample storage processing fresh sampling to preparation interval 10.0 months experimental protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_RNAseq_protocol_20210527.pdf extraction protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_RNAseq_protocol_20210527.pdf library preparation location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road library preparation location longitude 0.145393 decimal degrees library preparation location latitude 52.231532 decimal degrees library preparation date 2020-10-10 YYYY-MM-DD sequencing location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road sequencing location longitude 0.145393 decimal degrees sequencing location latitude 52.231532 decimal degrees sequencing date 2020-03-13 YYYY-MM-DD experiment target rna rna preparation 3 adapter ligation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD rna preparation 5 adapter ligation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD library generation pcr product isolation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD preparation reverse transcription protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD library generation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD read strand mate 2 sense rna purity 260-230 ratio 2.07 rna integrity number 10.0 ERX8902531 SSA_ROSLIN_AF_WP1_RNA_13_1 ERP136505 PRJEB51855 Stranded PE150 RNA-Seq experiment using polyA RNA selection and seqencing on a Novaseq 6000 ERS11209622 SAMEA9835705 D1301301 RNA-Seq TRANSCRIPTOMIC other NEBNext Ultra Directional RNA Library Prep Kit for Illumina E7420 manual Illumina NovaSeq 6000 project FAANG secondary project AQUA-FAANG assay type RNA-seq of coding RNA sample storage TRIzol, frozen sample storage processing fresh sampling to preparation interval 10.0 months experimental protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_RNAseq_protocol_20210527.pdf extraction protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_RNAseq_protocol_20210527.pdf library preparation location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road library preparation location longitude 0.145393 decimal degrees library preparation location latitude 52.231532 decimal degrees library preparation date 2020-10-10 YYYY-MM-DD sequencing location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road sequencing location longitude 0.145393 decimal degrees sequencing location latitude 52.231532 decimal degrees sequencing date 2020-03-13 YYYY-MM-DD experiment target rna rna preparation 3 adapter ligation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD rna preparation 5 adapter ligation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD library generation pcr product isolation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD preparation reverse transcription protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD library generation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD read strand mate 2 sense rna purity 260-230 ratio 0.53 rna integrity number 9.3 ERX8902532 SSA_ROSLIN_AF_WP1_RNA_13_2 ERP136505 PRJEB51855 Stranded PE150 RNA-Seq experiment using polyA RNA selection and seqencing on a Novaseq 6000 ERS11209638 SAMEA9835733 D1301302 RNA-Seq TRANSCRIPTOMIC other NEBNext Ultra Directional RNA Library Prep Kit for Illumina E7420 manual Illumina NovaSeq 6000 project FAANG secondary project AQUA-FAANG assay type RNA-seq of coding RNA sample storage TRIzol, frozen sample storage processing fresh sampling to preparation interval 10.0 months experimental protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_RNAseq_protocol_20210527.pdf extraction protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_RNAseq_protocol_20210527.pdf library preparation location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road library preparation location longitude 0.145393 decimal degrees library preparation location latitude 52.231532 decimal degrees library preparation date 2020-10-10 YYYY-MM-DD sequencing location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road sequencing location longitude 0.145393 decimal degrees sequencing location latitude 52.231532 decimal degrees sequencing date 2020-03-13 YYYY-MM-DD experiment target rna rna preparation 3 adapter ligation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD rna preparation 5 adapter ligation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD library generation pcr product isolation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD preparation reverse transcription protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD library generation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD read strand mate 2 sense rna purity 260-230 ratio 0.53 rna integrity number 9.3 ERX8902535 SSA_ROSLIN_AF_WP1_RNA_14_2 ERP136505 PRJEB51855 Stranded PE150 RNA-Seq experiment using polyA RNA selection and seqencing on a Novaseq 6000 ERS11209641 SAMEA9835735 D1301402 RNA-Seq TRANSCRIPTOMIC other NEBNext Ultra Directional RNA Library Prep Kit for Illumina E7420 manual Illumina NovaSeq 6000 project FAANG secondary project AQUA-FAANG assay type RNA-seq of coding RNA sample storage TRIzol, frozen sample storage processing fresh sampling to preparation interval 10.0 months experimental protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_RNAseq_protocol_20210527.pdf extraction protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_RNAseq_protocol_20210527.pdf library preparation location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road library preparation location longitude 0.145393 decimal degrees library preparation location latitude 52.231532 decimal degrees library preparation date 2020-10-10 YYYY-MM-DD sequencing location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road sequencing location longitude 0.145393 decimal degrees sequencing location latitude 52.231532 decimal degrees sequencing date 2020-03-13 YYYY-MM-DD experiment target rna rna preparation 3 adapter ligation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD rna preparation 5 adapter ligation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD library generation pcr product isolation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD preparation reverse transcription protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD library generation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD read strand mate 2 sense rna purity 260-230 ratio 2.07 rna integrity number 9.9 ERX8902536 SSA_ROSLIN_AF_WP1_RNA_14_3 ERP136505 PRJEB51855 Stranded PE150 RNA-Seq experiment using polyA RNA selection and seqencing on a Novaseq 6000 ERS11209626 SAMEA9835763 D1301403 RNA-Seq TRANSCRIPTOMIC other NEBNext Ultra Directional RNA Library Prep Kit for Illumina E7420 manual Illumina NovaSeq 6000 project FAANG secondary project AQUA-FAANG assay type RNA-seq of coding RNA sample storage TRIzol, frozen sample storage processing fresh sampling to preparation interval 10.0 months experimental protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_RNAseq_protocol_20210527.pdf extraction protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_RNAseq_protocol_20210527.pdf library preparation location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road library preparation location longitude 0.145393 decimal degrees library preparation location latitude 52.231532 decimal degrees library preparation date 2020-10-10 YYYY-MM-DD sequencing location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road sequencing location longitude 0.145393 decimal degrees sequencing location latitude 52.231532 decimal degrees sequencing date 2020-03-13 YYYY-MM-DD experiment target rna rna preparation 3 adapter ligation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD rna preparation 5 adapter ligation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD library generation pcr product isolation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD preparation reverse transcription protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD library generation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD read strand mate 2 sense rna purity 260-230 ratio 2.26 rna integrity number 9.8 ERX8902537 SSA_ROSLIN_AF_WP1_ATAC_01_1 ERP136504 PRJEB51854 ATAC-seq genomic paired-end experiment using Illumina's IDT UDI multiplexing and sequencing on Novaseq 6000 with PE150 ERS11209642 SAMEA9835779 D1100101 ATAC-seq GENOMIC other https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_ATACseq_protocol_part2_20210527.pdf Illumina NovaSeq 6000 project FAANG secondary project AQUA-FAANG assay type ATAC-seq sample storage frozen, -70 freezer sample storage processing cryopreservation, other sampling to preparation interval 10.0 months experimental protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_ATACseq_protocol_part2_20210527.pdf extraction protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_ATACseq_protocol_part2_20210527.pdf library preparation location The University of Edinburgh, Easter Bush Campus, The Roslin Institute, Midlothian EH25 9RG library preparation location longitude -3.19917081077908 decimal degrees library preparation location latitude 55.8657492911024 decimal degrees library preparation date 2020-10-10 YYYY-MM-DD sequencing location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road sequencing location longitude 0.145393 decimal degrees sequencing location latitude 52.231532 decimal degrees sequencing date 2020-10-10 YYYY-MM-DD experiment target open_chromatin_region transposase protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_ATACseq_protocol_part2_20210527.pdf ERX8902538 SSA_ROSLIN_AF_WP1_ATAC_01_2 ERP136504 PRJEB51854 ATAC-seq genomic paired-end experiment using Illumina's IDT UDI multiplexing and sequencing on Novaseq 6000 with PE150 ERS11209587 SAMEA9835786 D1100102 ATAC-seq GENOMIC other https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_ATACseq_protocol_part2_20210527.pdf Illumina NovaSeq 6000 project FAANG secondary project AQUA-FAANG assay type ATAC-seq sample storage frozen, -70 freezer sample storage processing cryopreservation, other sampling to preparation interval 10.0 months experimental protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_ATACseq_protocol_part2_20210527.pdf extraction protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_ATACseq_protocol_part2_20210527.pdf library preparation location The University of Edinburgh, Easter Bush Campus, The Roslin Institute, Midlothian EH25 9RG library preparation location longitude -3.19917081077908 decimal degrees library preparation location latitude 55.8657492911024 decimal degrees library preparation date 2020-10-10 YYYY-MM-DD sequencing location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road sequencing location longitude 0.145393 decimal degrees sequencing location latitude 52.231532 decimal degrees sequencing date 2020-10-10 YYYY-MM-DD experiment target open_chromatin_region transposase protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_ATACseq_protocol_part2_20210527.pdf ERX8902539 SSA_ROSLIN_AF_WP1_ATAC_01_3 ERP136504 PRJEB51854 ATAC-seq genomic paired-end experiment using Illumina's IDT UDI multiplexing and sequencing on Novaseq 6000 with PE150 ERS11209616 SAMEA9835793 D1100103 ATAC-seq GENOMIC other https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_ATACseq_protocol_part2_20210527.pdf Illumina NovaSeq 6000 project FAANG secondary project AQUA-FAANG assay type ATAC-seq sample storage frozen, -70 freezer sample storage processing cryopreservation, other sampling to preparation interval 10.0 months experimental protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_ATACseq_protocol_part2_20210527.pdf extraction protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_ATACseq_protocol_part2_20210527.pdf library preparation location The University of Edinburgh, Easter Bush Campus, The Roslin Institute, Midlothian EH25 9RG library preparation location longitude -3.19917081077908 decimal degrees library preparation location latitude 55.8657492911024 decimal degrees library preparation date 2020-10-10 YYYY-MM-DD sequencing location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road sequencing location longitude 0.145393 decimal degrees sequencing location latitude 52.231532 decimal degrees sequencing date 2020-10-10 YYYY-MM-DD experiment target open_chromatin_region transposase protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_ATACseq_protocol_part2_20210527.pdf ERX8902540 SSA_ROSLIN_AF_WP1_ATAC_02_1 ERP136504 PRJEB51854 ATAC-seq genomic paired-end experiment using Illumina's IDT UDI multiplexing and sequencing on Novaseq 6000 with PE150 ERS11209597 SAMEA9835780 D1100201 ATAC-seq GENOMIC other https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_ATACseq_protocol_part2_20210527.pdf Illumina NovaSeq 6000 project FAANG secondary project AQUA-FAANG assay type ATAC-seq sample storage frozen, -70 freezer sample storage processing cryopreservation, other sampling to preparation interval 10.0 months experimental protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_ATACseq_protocol_part2_20210527.pdf extraction protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_ATACseq_protocol_part2_20210527.pdf library preparation location The University of Edinburgh, Easter Bush Campus, The Roslin Institute, Midlothian EH25 9RG library preparation location longitude -3.19917081077908 decimal degrees library preparation location latitude 55.8657492911024 decimal degrees library preparation date 2020-10-10 YYYY-MM-DD sequencing location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road sequencing location longitude 0.145393 decimal degrees sequencing location latitude 52.231532 decimal degrees sequencing date 2020-10-10 YYYY-MM-DD experiment target open_chromatin_region transposase protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_ATACseq_protocol_part2_20210527.pdf ERX8902541 SSA_ROSLIN_AF_WP1_ATAC_02_2 ERP136504 PRJEB51854 ATAC-seq genomic paired-end experiment using Illumina's IDT UDI multiplexing and sequencing on Novaseq 6000 with PE150 ERS11209591 SAMEA9835787 D1100202 ATAC-seq GENOMIC other https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_ATACseq_protocol_part2_20210527.pdf Illumina NovaSeq 6000 project FAANG secondary project AQUA-FAANG assay type ATAC-seq sample storage frozen, -70 freezer sample storage processing cryopreservation, other sampling to preparation interval 10.0 months experimental protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_ATACseq_protocol_part2_20210527.pdf extraction protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_ATACseq_protocol_part2_20210527.pdf library preparation location The University of Edinburgh, Easter Bush Campus, The Roslin Institute, Midlothian EH25 9RG library preparation location longitude -3.19917081077908 decimal degrees library preparation location latitude 55.8657492911024 decimal degrees library preparation date 2020-10-10 YYYY-MM-DD sequencing location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road sequencing location longitude 0.145393 decimal degrees sequencing location latitude 52.231532 decimal degrees sequencing date 2020-10-10 YYYY-MM-DD experiment target open_chromatin_region transposase protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_ATACseq_protocol_part2_20210527.pdf ERX8902542 SSA_ROSLIN_AF_WP1_ATAC_02_3 ERP136504 PRJEB51854 ATAC-seq genomic paired-end experiment using Illumina's IDT UDI multiplexing and sequencing on Novaseq 6000 with PE150 ERS11209620 SAMEA9835794 D1100203 ATAC-seq GENOMIC other https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_ATACseq_protocol_part2_20210527.pdf Illumina NovaSeq 6000 project FAANG secondary project AQUA-FAANG assay type ATAC-seq sample storage frozen, -70 freezer sample storage processing cryopreservation, other sampling to preparation interval 10.0 months experimental protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_ATACseq_protocol_part2_20210527.pdf extraction protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_ATACseq_protocol_part2_20210527.pdf library preparation location The University of Edinburgh, Easter Bush Campus, The Roslin Institute, Midlothian EH25 9RG library preparation location longitude -3.19917081077908 decimal degrees library preparation location latitude 55.8657492911024 decimal degrees library preparation date 2020-10-10 YYYY-MM-DD sequencing location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road sequencing location longitude 0.145393 decimal degrees sequencing location latitude 52.231532 decimal degrees sequencing date 2020-10-10 YYYY-MM-DD experiment target open_chromatin_region transposase protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_ATACseq_protocol_part2_20210527.pdf ERX8902544 SSA_ROSLIN_AF_WP1_ATAC_03_2 ERP136504 PRJEB51854 ATAC-seq genomic paired-end experiment using Illumina's IDT UDI multiplexing and sequencing on Novaseq 6000 with PE150 ERS11209590 SAMEA9835788 D1100302 ATAC-seq GENOMIC other https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_ATACseq_protocol_part2_20210527.pdf Illumina NovaSeq 6000 project FAANG secondary project AQUA-FAANG assay type ATAC-seq sample storage frozen, -70 freezer sample storage processing cryopreservation, other sampling to preparation interval 10.0 months experimental protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_ATACseq_protocol_part2_20210527.pdf extraction protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_ATACseq_protocol_part2_20210527.pdf library preparation location The University of Edinburgh, Easter Bush Campus, The Roslin Institute, Midlothian EH25 9RG library preparation location longitude -3.19917081077908 decimal degrees library preparation location latitude 55.8657492911024 decimal degrees library preparation date 2020-10-10 YYYY-MM-DD sequencing location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road sequencing location longitude 0.145393 decimal degrees sequencing location latitude 52.231532 decimal degrees sequencing date 2020-10-10 YYYY-MM-DD experiment target open_chromatin_region transposase protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_ATACseq_protocol_part2_20210527.pdf ERX8902545 SSA_ROSLIN_AF_WP1_ATAC_03_3 ERP136504 PRJEB51854 ATAC-seq genomic paired-end experiment using Illumina's IDT UDI multiplexing and sequencing on Novaseq 6000 with PE150 ERS11209619 SAMEA9835795 D1100303 ATAC-seq GENOMIC other https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_ATACseq_protocol_part2_20210527.pdf Illumina NovaSeq 6000 project FAANG secondary project AQUA-FAANG assay type ATAC-seq sample storage frozen, -70 freezer sample storage processing cryopreservation, other sampling to preparation interval 10.0 months experimental protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_ATACseq_protocol_part2_20210527.pdf extraction protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_ATACseq_protocol_part2_20210527.pdf library preparation location The University of Edinburgh, Easter Bush Campus, The Roslin Institute, Midlothian EH25 9RG library preparation location longitude -3.19917081077908 decimal degrees library preparation location latitude 55.8657492911024 decimal degrees library preparation date 2020-10-10 YYYY-MM-DD sequencing location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road sequencing location longitude 0.145393 decimal degrees sequencing location latitude 52.231532 decimal degrees sequencing date 2020-10-10 YYYY-MM-DD experiment target open_chromatin_region transposase protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_ATACseq_protocol_part2_20210527.pdf ERX8902547 SSA_ROSLIN_AF_WP1_ATAC_04_2 ERP136504 PRJEB51854 ATAC-seq genomic paired-end experiment using Illumina's IDT UDI multiplexing and sequencing on Novaseq 6000 with PE150 ERS11209594 SAMEA9835789 D1100402 ATAC-seq GENOMIC other https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_ATACseq_protocol_part2_20210527.pdf Illumina NovaSeq 6000 project FAANG secondary project AQUA-FAANG assay type ATAC-seq sample storage frozen, -70 freezer sample storage processing cryopreservation, other sampling to preparation interval 10.0 months experimental protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_ATACseq_protocol_part2_20210527.pdf extraction protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_ATACseq_protocol_part2_20210527.pdf library preparation location The University of Edinburgh, Easter Bush Campus, The Roslin Institute, Midlothian EH25 9RG library preparation location longitude -3.19917081077908 decimal degrees library preparation location latitude 55.8657492911024 decimal degrees library preparation date 2020-10-10 YYYY-MM-DD sequencing location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road sequencing location longitude 0.145393 decimal degrees sequencing location latitude 52.231532 decimal degrees sequencing date 2020-10-10 YYYY-MM-DD experiment target open_chromatin_region transposase protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_ATACseq_protocol_part2_20210527.pdf ERX8902550 SSA_ROSLIN_AF_WP1_ATAC_05_2 ERP136504 PRJEB51854 ATAC-seq genomic paired-end experiment using Illumina's IDT UDI multiplexing and sequencing on Novaseq 6000 with PE150 ERS11209615 SAMEA9835790 D1100502 ATAC-seq GENOMIC other https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_ATACseq_protocol_part2_20210527.pdf Illumina NovaSeq 6000 project FAANG secondary project AQUA-FAANG assay type ATAC-seq sample storage frozen, -70 freezer sample storage processing cryopreservation, other sampling to preparation interval 10.0 months experimental protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_ATACseq_protocol_part2_20210527.pdf extraction protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_ATACseq_protocol_part2_20210527.pdf library preparation location The University of Edinburgh, Easter Bush Campus, The Roslin Institute, Midlothian EH25 9RG library preparation location longitude -3.19917081077908 decimal degrees library preparation location latitude 55.8657492911024 decimal degrees library preparation date 2020-10-10 YYYY-MM-DD sequencing location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road sequencing location longitude 0.145393 decimal degrees sequencing location latitude 52.231532 decimal degrees sequencing date 2020-10-10 YYYY-MM-DD experiment target open_chromatin_region transposase protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_ATACseq_protocol_part2_20210527.pdf ERX8902551 SSA_ROSLIN_AF_WP1_ATAC_05_3 ERP136504 PRJEB51854 ATAC-seq genomic paired-end experiment using Illumina's IDT UDI multiplexing and sequencing on Novaseq 6000 with PE150 ERS11209603 SAMEA9835797 D1100503 ATAC-seq GENOMIC other https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_ATACseq_protocol_part2_20210527.pdf Illumina NovaSeq 6000 project FAANG secondary project AQUA-FAANG assay type ATAC-seq sample storage frozen, -70 freezer sample storage processing cryopreservation, other sampling to preparation interval 10.0 months experimental protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_ATACseq_protocol_part2_20210527.pdf extraction protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_ATACseq_protocol_part2_20210527.pdf library preparation location The University of Edinburgh, Easter Bush Campus, The Roslin Institute, Midlothian EH25 9RG library preparation location longitude -3.19917081077908 decimal degrees library preparation location latitude 55.8657492911024 decimal degrees library preparation date 2020-10-10 YYYY-MM-DD sequencing location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road sequencing location longitude 0.145393 decimal degrees sequencing location latitude 52.231532 decimal degrees sequencing date 2020-10-10 YYYY-MM-DD experiment target open_chromatin_region transposase protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_ATACseq_protocol_part2_20210527.pdf ERX8902552 SSA_ROSLIN_AF_WP1_ATAC_06_1 ERP136504 PRJEB51854 ATAC-seq genomic paired-end experiment using Illumina's IDT UDI multiplexing and sequencing on Novaseq 6000 with PE150 ERS11209600 SAMEA9835784 D1100601 ATAC-seq GENOMIC other https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_ATACseq_protocol_part2_20210527.pdf Illumina NovaSeq 6000 project FAANG secondary project AQUA-FAANG assay type ATAC-seq sample storage frozen, -70 freezer sample storage processing cryopreservation, other sampling to preparation interval 10.0 months experimental protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_ATACseq_protocol_part2_20210527.pdf extraction protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_ATACseq_protocol_part2_20210527.pdf library preparation location The University of Edinburgh, Easter Bush Campus, The Roslin Institute, Midlothian EH25 9RG library preparation location longitude -3.19917081077908 decimal degrees library preparation location latitude 55.8657492911024 decimal degrees library preparation date 2020-10-10 YYYY-MM-DD sequencing location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road sequencing location longitude 0.145393 decimal degrees sequencing location latitude 52.231532 decimal degrees sequencing date 2020-10-10 YYYY-MM-DD experiment target open_chromatin_region transposase protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_ATACseq_protocol_part2_20210527.pdf ERX8902553 SSA_ROSLIN_AF_WP1_ATAC_06_2 ERP136504 PRJEB51854 ATAC-seq genomic paired-end experiment using Illumina's IDT UDI multiplexing and sequencing on Novaseq 6000 with PE150 ERS11209614 SAMEA9835791 D1100602 ATAC-seq GENOMIC other https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_ATACseq_protocol_part2_20210527.pdf Illumina NovaSeq 6000 project FAANG secondary project AQUA-FAANG assay type ATAC-seq sample storage frozen, -70 freezer sample storage processing cryopreservation, other sampling to preparation interval 10.0 months experimental protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_ATACseq_protocol_part2_20210527.pdf extraction protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_ATACseq_protocol_part2_20210527.pdf library preparation location The University of Edinburgh, Easter Bush Campus, The Roslin Institute, Midlothian EH25 9RG library preparation location longitude -3.19917081077908 decimal degrees library preparation location latitude 55.8657492911024 decimal degrees library preparation date 2020-10-10 YYYY-MM-DD sequencing location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road sequencing location longitude 0.145393 decimal degrees sequencing location latitude 52.231532 decimal degrees sequencing date 2020-10-10 YYYY-MM-DD experiment target open_chromatin_region transposase protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_ATACseq_protocol_part2_20210527.pdf ERX8902554 SSA_ROSLIN_AF_WP1_ATAC_06_3 ERP136504 PRJEB51854 ATAC-seq genomic paired-end experiment using Illumina's IDT UDI multiplexing and sequencing on Novaseq 6000 with PE150 ERS11209609 SAMEA9835798 D1100603 ATAC-seq GENOMIC other https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_ATACseq_protocol_part2_20210527.pdf Illumina NovaSeq 6000 project FAANG secondary project AQUA-FAANG assay type ATAC-seq sample storage frozen, -70 freezer sample storage processing cryopreservation, other sampling to preparation interval 10.0 months experimental protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_ATACseq_protocol_part2_20210527.pdf extraction protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_ATACseq_protocol_part2_20210527.pdf library preparation location The University of Edinburgh, Easter Bush Campus, The Roslin Institute, Midlothian EH25 9RG library preparation location longitude -3.19917081077908 decimal degrees library preparation location latitude 55.8657492911024 decimal degrees library preparation date 2020-10-10 YYYY-MM-DD sequencing location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road sequencing location longitude 0.145393 decimal degrees sequencing location latitude 52.231532 decimal degrees sequencing date 2020-10-10 YYYY-MM-DD experiment target open_chromatin_region transposase protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_ATACseq_protocol_part2_20210527.pdf ERX8902555 SSA_ROSLIN_AF_WP1_ATAC_07_1 ERP136504 PRJEB51854 ATAC-seq genomic paired-end experiment using Illumina's IDT UDI multiplexing and sequencing on Novaseq 6000 with PE150 ERS14678312 SAMEA112653335 D1100701 ATAC-seq GENOMIC other https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_ATACseq_protocol_part2_20210527.pdf Illumina NovaSeq 6000 project FAANG secondary project AQUA-FAANG assay type ATAC-seq sample storage frozen, -70 freezer sample storage processing cryopreservation, other sampling to preparation interval 10.0 months experimental protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_ATACseq_protocol_part2_20210527.pdf extraction protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_ATACseq_protocol_part2_20210527.pdf library preparation location The University of Edinburgh, Easter Bush Campus, The Roslin Institute, Midlothian EH25 9RG library preparation location longitude -3.19917081077908 decimal degrees library preparation location latitude 55.8657492911024 decimal degrees library preparation date 2020-10-10 YYYY-MM-DD sequencing location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road sequencing location longitude 0.145393 decimal degrees sequencing location latitude 52.231532 decimal degrees sequencing date 2020-10-10 YYYY-MM-DD experiment target open_chromatin_region transposase protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_ATACseq_protocol_part2_20210527.pdf ERX8902556 SSA_ROSLIN_AF_WP1_ATAC_07_2 ERP136504 PRJEB51854 ATAC-seq genomic paired-end experiment using Illumina's IDT UDI multiplexing and sequencing on Novaseq 6000 with PE150 ERS14678313 SAMEA112653336 D1100702 ATAC-seq GENOMIC other https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_ATACseq_protocol_part2_20210527.pdf Illumina NovaSeq 6000 project FAANG secondary project AQUA-FAANG assay type ATAC-seq sample storage frozen, -70 freezer sample storage processing cryopreservation, other sampling to preparation interval 10.0 months experimental protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_ATACseq_protocol_part2_20210527.pdf extraction protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_ATACseq_protocol_part2_20210527.pdf library preparation location The University of Edinburgh, Easter Bush Campus, The Roslin Institute, Midlothian EH25 9RG library preparation location longitude -3.19917081077908 decimal degrees library preparation location latitude 55.8657492911024 decimal degrees library preparation date 2020-10-10 YYYY-MM-DD sequencing location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road sequencing location longitude 0.145393 decimal degrees sequencing location latitude 52.231532 decimal degrees sequencing date 2020-10-10 YYYY-MM-DD experiment target open_chromatin_region transposase protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_ATACseq_protocol_part2_20210527.pdf ERX8902557 SSA_ROSLIN_AF_WP1_ATAC_07_3 ERP136504 PRJEB51854 ATAC-seq genomic paired-end experiment using Illumina's IDT UDI multiplexing and sequencing on Novaseq 6000 with PE150 ERS14678314 SAMEA112653337 D1100703 ATAC-seq GENOMIC other https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_ATACseq_protocol_part2_20210527.pdf Illumina NovaSeq 6000 project FAANG secondary project AQUA-FAANG assay type ATAC-seq sample storage frozen, -70 freezer sample storage processing cryopreservation, other sampling to preparation interval 10.0 months experimental protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_ATACseq_protocol_part2_20210527.pdf extraction protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_ATACseq_protocol_part2_20210527.pdf library preparation location The University of Edinburgh, Easter Bush Campus, The Roslin Institute, Midlothian EH25 9RG library preparation location longitude -3.19917081077908 decimal degrees library preparation location latitude 55.8657492911024 decimal degrees library preparation date 2020-10-10 YYYY-MM-DD sequencing location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road sequencing location longitude 0.145393 decimal degrees sequencing location latitude 52.231532 decimal degrees sequencing date 2020-10-10 YYYY-MM-DD experiment target open_chromatin_region transposase protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_ATACseq_protocol_part2_20210527.pdf ERX8902495 SSA_ROSLIN_AF_WP1_RNA_01_1 ERP136505 PRJEB51855 Stranded PE150 RNA-Seq experiment using polyA RNA selection and seqencing on a Novaseq 6000 ERS11209634 SAMEA9835698 D1300101 RNA-Seq TRANSCRIPTOMIC other NEBNext Ultra Directional RNA Library Prep Kit for Illumina E7420 manual Illumina NovaSeq 6000 project FAANG secondary project AQUA-FAANG assay type RNA-seq of coding RNA sample storage TRIzol, frozen sample storage processing fresh sampling to preparation interval 10.0 months experimental protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_RNAseq_protocol_20210527.pdf extraction protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_RNAseq_protocol_20210527.pdf library preparation location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road library preparation location longitude 0.145393 decimal degrees library preparation location latitude 52.231532 decimal degrees library preparation date 2020-10-10 YYYY-MM-DD sequencing location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road sequencing location longitude 0.145393 decimal degrees sequencing location latitude 52.231532 decimal degrees sequencing date 2020-03-13 YYYY-MM-DD experiment target rna rna preparation 3 adapter ligation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD rna preparation 5 adapter ligation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD library generation pcr product isolation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD preparation reverse transcription protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD library generation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD read strand mate 2 sense rna purity 260-230 ratio 1.45 rna integrity number 8.9 ERX8902496 SSA_ROSLIN_AF_WP1_RNA_01_2 ERP136505 PRJEB51855 Stranded PE150 RNA-Seq experiment using polyA RNA selection and seqencing on a Novaseq 6000 ERS11209629 SAMEA9835726 D1300102 RNA-Seq TRANSCRIPTOMIC other NEBNext Ultra Directional RNA Library Prep Kit for Illumina E7420 manual Illumina NovaSeq 6000 project FAANG secondary project AQUA-FAANG assay type RNA-seq of coding RNA sample storage TRIzol, frozen sample storage processing fresh sampling to preparation interval 10.0 months experimental protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_RNAseq_protocol_20210527.pdf extraction protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_RNAseq_protocol_20210527.pdf library preparation location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road library preparation location longitude 0.145393 decimal degrees library preparation location latitude 52.231532 decimal degrees library preparation date 2020-10-10 YYYY-MM-DD sequencing location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road sequencing location longitude 0.145393 decimal degrees sequencing location latitude 52.231532 decimal degrees sequencing date 2020-03-13 YYYY-MM-DD experiment target rna rna preparation 3 adapter ligation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD rna preparation 5 adapter ligation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD library generation pcr product isolation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD preparation reverse transcription protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD library generation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD read strand mate 2 sense rna purity 260-230 ratio 1.3 rna integrity number 8.7 ERX8902502 SSA_ROSLIN_AF_WP1_RNA_03_2 ERP136505 PRJEB51855 Stranded PE150 RNA-Seq experiment using polyA RNA selection and seqencing on a Novaseq 6000 ERS11209636 SAMEA9835730 D1300302 RNA-Seq TRANSCRIPTOMIC other NEBNext Ultra Directional RNA Library Prep Kit for Illumina E7420 manual Illumina NovaSeq 6000 project FAANG secondary project AQUA-FAANG assay type RNA-seq of coding RNA sample storage TRIzol, frozen sample storage processing fresh sampling to preparation interval 10.0 months experimental protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_RNAseq_protocol_20210527.pdf extraction protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_RNAseq_protocol_20210527.pdf library preparation location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road library preparation location longitude 0.145393 decimal degrees library preparation location latitude 52.231532 decimal degrees library preparation date 2020-10-10 YYYY-MM-DD sequencing location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road sequencing location longitude 0.145393 decimal degrees sequencing location latitude 52.231532 decimal degrees sequencing date 2020-03-13 YYYY-MM-DD experiment target rna rna preparation 3 adapter ligation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD rna preparation 5 adapter ligation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD library generation pcr product isolation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD preparation reverse transcription protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD library generation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD read strand mate 2 sense rna purity 260-230 ratio 1.89 rna integrity number 9.7 ERX8902505 SSA_ROSLIN_AF_WP1_RNA_04_2 ERP136505 PRJEB51855 Stranded PE150 RNA-Seq experiment using polyA RNA selection and seqencing on a Novaseq 6000 ERS11209639 SAMEA9835732 D1300402 RNA-Seq TRANSCRIPTOMIC other NEBNext Ultra Directional RNA Library Prep Kit for Illumina E7420 manual Illumina NovaSeq 6000 project FAANG secondary project AQUA-FAANG assay type RNA-seq of coding RNA sample storage TRIzol, frozen sample storage processing fresh sampling to preparation interval 10.0 months experimental protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_RNAseq_protocol_20210527.pdf extraction protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_RNAseq_protocol_20210527.pdf library preparation location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road library preparation location longitude 0.145393 decimal degrees library preparation location latitude 52.231532 decimal degrees library preparation date 2020-10-10 YYYY-MM-DD sequencing location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road sequencing location longitude 0.145393 decimal degrees sequencing location latitude 52.231532 decimal degrees sequencing date 2020-03-13 YYYY-MM-DD experiment target rna rna preparation 3 adapter ligation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD rna preparation 5 adapter ligation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD library generation pcr product isolation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD preparation reverse transcription protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD library generation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD read strand mate 2 sense rna purity 260-230 ratio 1.34 rna integrity number 9.5 ERX8902508 SSA_ROSLIN_AF_WP1_RNA_05_2 ERP136505 PRJEB51855 Stranded PE150 RNA-Seq experiment using polyA RNA selection and seqencing on a Novaseq 6000 ERS11209643 SAMEA9835734 D1300502 RNA-Seq TRANSCRIPTOMIC other NEBNext Ultra Directional RNA Library Prep Kit for Illumina E7420 manual Illumina NovaSeq 6000 project FAANG secondary project AQUA-FAANG assay type RNA-seq of coding RNA sample storage TRIzol, frozen sample storage processing fresh sampling to preparation interval 10.0 months experimental protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_RNAseq_protocol_20210527.pdf extraction protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_RNAseq_protocol_20210527.pdf library preparation location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road library preparation location longitude 0.145393 decimal degrees library preparation location latitude 52.231532 decimal degrees library preparation date 2020-10-10 YYYY-MM-DD sequencing location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road sequencing location longitude 0.145393 decimal degrees sequencing location latitude 52.231532 decimal degrees sequencing date 2020-03-13 YYYY-MM-DD experiment target rna rna preparation 3 adapter ligation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD rna preparation 5 adapter ligation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD library generation pcr product isolation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD preparation reverse transcription protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD library generation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD read strand mate 2 sense rna purity 260-230 ratio 2.06 rna integrity number 9.8 ERX8902511 SSA_ROSLIN_AF_WP1_RNA_06_2 ERP136505 PRJEB51855 Stranded PE150 RNA-Seq experiment using polyA RNA selection and seqencing on a Novaseq 6000 ERS11209644 SAMEA9835736 D1300602 RNA-Seq TRANSCRIPTOMIC other NEBNext Ultra Directional RNA Library Prep Kit for Illumina E7420 manual Illumina NovaSeq 6000 project FAANG secondary project AQUA-FAANG assay type RNA-seq of coding RNA sample storage TRIzol, frozen sample storage processing fresh sampling to preparation interval 10.0 months experimental protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_RNAseq_protocol_20210527.pdf extraction protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_RNAseq_protocol_20210527.pdf library preparation location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road library preparation location longitude 0.145393 decimal degrees library preparation location latitude 52.231532 decimal degrees library preparation date 2020-10-10 YYYY-MM-DD sequencing location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road sequencing location longitude 0.145393 decimal degrees sequencing location latitude 52.231532 decimal degrees sequencing date 2020-03-13 YYYY-MM-DD experiment target rna rna preparation 3 adapter ligation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD rna preparation 5 adapter ligation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD library generation pcr product isolation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD preparation reverse transcription protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD library generation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD read strand mate 2 sense rna purity 260-230 ratio 2.33 rna integrity number 7.8 ERX8902515 SSA_ROSLIN_AF_WP1_RNA_07_3 ERP136505 PRJEB51855 Stranded PE150 RNA-Seq experiment using polyA RNA selection and seqencing on a Novaseq 6000 ERS11209618 SAMEA9835751 D1300703 RNA-Seq TRANSCRIPTOMIC other NEBNext Ultra Directional RNA Library Prep Kit for Illumina E7420 manual Illumina NovaSeq 6000 project FAANG secondary project AQUA-FAANG assay type RNA-seq of coding RNA sample storage TRIzol, frozen sample storage processing fresh sampling to preparation interval 10.0 months experimental protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_RNAseq_protocol_20210527.pdf extraction protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_RNAseq_protocol_20210527.pdf library preparation location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road library preparation location longitude 0.145393 decimal degrees library preparation location latitude 52.231532 decimal degrees library preparation date 2020-10-10 YYYY-MM-DD sequencing location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road sequencing location longitude 0.145393 decimal degrees sequencing location latitude 52.231532 decimal degrees sequencing date 2020-03-13 YYYY-MM-DD experiment target rna rna preparation 3 adapter ligation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD rna preparation 5 adapter ligation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD library generation pcr product isolation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD preparation reverse transcription protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD library generation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD read strand mate 2 sense rna purity 260-230 ratio 1.44 ERX8902519 SSA_ROSLIN_AF_WP1_RNA_09_1 ERP136505 PRJEB51855 Stranded PE150 RNA-Seq experiment using polyA RNA selection and seqencing on a Novaseq 6000 ERS11209637 SAMEA9835697 D1300901 RNA-Seq TRANSCRIPTOMIC other NEBNext Ultra Directional RNA Library Prep Kit for Illumina E7420 manual Illumina NovaSeq 6000 project FAANG secondary project AQUA-FAANG assay type RNA-seq of coding RNA sample storage TRIzol, frozen sample storage processing fresh sampling to preparation interval 10.0 months experimental protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_RNAseq_protocol_20210527.pdf extraction protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_RNAseq_protocol_20210527.pdf library preparation location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road library preparation location longitude 0.145393 decimal degrees library preparation location latitude 52.231532 decimal degrees library preparation date 2020-10-10 YYYY-MM-DD sequencing location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road sequencing location longitude 0.145393 decimal degrees sequencing location latitude 52.231532 decimal degrees sequencing date 2020-03-13 YYYY-MM-DD experiment target rna rna preparation 3 adapter ligation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD rna preparation 5 adapter ligation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD library generation pcr product isolation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD preparation reverse transcription protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD library generation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD read strand mate 2 sense rna purity 260-230 ratio 1.22 rna integrity number 8.3 ERX8902522 SSA_ROSLIN_AF_WP1_RNA_10_1 ERP136505 PRJEB51855 Stranded PE150 RNA-Seq experiment using polyA RNA selection and seqencing on a Novaseq 6000 ERS11209640 SAMEA9835699 D1301001 RNA-Seq TRANSCRIPTOMIC other NEBNext Ultra Directional RNA Library Prep Kit for Illumina E7420 manual Illumina NovaSeq 6000 project FAANG secondary project AQUA-FAANG assay type RNA-seq of coding RNA sample storage TRIzol, frozen sample storage processing fresh sampling to preparation interval 10.0 months experimental protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_RNAseq_protocol_20210527.pdf extraction protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_RNAseq_protocol_20210527.pdf library preparation location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road library preparation location longitude 0.145393 decimal degrees library preparation location latitude 52.231532 decimal degrees library preparation date 2020-10-10 YYYY-MM-DD sequencing location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road sequencing location longitude 0.145393 decimal degrees sequencing location latitude 52.231532 decimal degrees sequencing date 2020-03-13 YYYY-MM-DD experiment target rna rna preparation 3 adapter ligation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD rna preparation 5 adapter ligation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD library generation pcr product isolation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD preparation reverse transcription protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD library generation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD read strand mate 2 sense rna purity 260-230 ratio 1.37 rna integrity number 6.9 ERX8902530 SSA_ROSLIN_AF_WP1_RNA_12_3 ERP136505 PRJEB51855 Stranded PE150 RNA-Seq experiment using polyA RNA selection and seqencing on a Novaseq 6000 ERS11209612 SAMEA9835759 D1301203 RNA-Seq TRANSCRIPTOMIC other NEBNext Ultra Directional RNA Library Prep Kit for Illumina E7420 manual Illumina NovaSeq 6000 project FAANG secondary project AQUA-FAANG assay type RNA-seq of coding RNA sample storage TRIzol, frozen sample storage processing fresh sampling to preparation interval 10.0 months experimental protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_RNAseq_protocol_20210527.pdf extraction protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_RNAseq_protocol_20210527.pdf library preparation location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road library preparation location longitude 0.145393 decimal degrees library preparation location latitude 52.231532 decimal degrees library preparation date 2020-10-10 YYYY-MM-DD sequencing location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road sequencing location longitude 0.145393 decimal degrees sequencing location latitude 52.231532 decimal degrees sequencing date 2020-03-13 YYYY-MM-DD experiment target rna rna preparation 3 adapter ligation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD rna preparation 5 adapter ligation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD library generation pcr product isolation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD preparation reverse transcription protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD library generation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD read strand mate 2 sense rna purity 260-230 ratio 2.33 rna integrity number 10.0 ERX8902533 SSA_ROSLIN_AF_WP1_RNA_13_3 ERP136505 PRJEB51855 Stranded PE150 RNA-Seq experiment using polyA RNA selection and seqencing on a Novaseq 6000 ERS11209633 SAMEA9835761 D1301303 RNA-Seq TRANSCRIPTOMIC other NEBNext Ultra Directional RNA Library Prep Kit for Illumina E7420 manual Illumina NovaSeq 6000 project FAANG secondary project AQUA-FAANG assay type RNA-seq of coding RNA sample storage TRIzol, frozen sample storage processing fresh sampling to preparation interval 10.0 months experimental protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_RNAseq_protocol_20210527.pdf extraction protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_RNAseq_protocol_20210527.pdf library preparation location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road library preparation location longitude 0.145393 decimal degrees library preparation location latitude 52.231532 decimal degrees library preparation date 2020-10-10 YYYY-MM-DD sequencing location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road sequencing location longitude 0.145393 decimal degrees sequencing location latitude 52.231532 decimal degrees sequencing date 2020-03-13 YYYY-MM-DD experiment target rna rna preparation 3 adapter ligation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD rna preparation 5 adapter ligation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD library generation pcr product isolation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD preparation reverse transcription protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD library generation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD read strand mate 2 sense rna purity 260-230 ratio 1.98 rna integrity number 9.8 ERX8902534 SSA_ROSLIN_AF_WP1_RNA_14_1 ERP136505 PRJEB51855 Stranded PE150 RNA-Seq experiment using polyA RNA selection and seqencing on a Novaseq 6000 ERS11209624 SAMEA9835707 D1301401 RNA-Seq TRANSCRIPTOMIC other NEBNext Ultra Directional RNA Library Prep Kit for Illumina E7420 manual Illumina NovaSeq 6000 project FAANG secondary project AQUA-FAANG assay type RNA-seq of coding RNA sample storage TRIzol, frozen sample storage processing fresh sampling to preparation interval 10.0 months experimental protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_RNAseq_protocol_20210527.pdf extraction protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_RNAseq_protocol_20210527.pdf library preparation location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road library preparation location longitude 0.145393 decimal degrees library preparation location latitude 52.231532 decimal degrees library preparation date 2020-10-10 YYYY-MM-DD sequencing location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road sequencing location longitude 0.145393 decimal degrees sequencing location latitude 52.231532 decimal degrees sequencing date 2020-03-13 YYYY-MM-DD experiment target rna rna preparation 3 adapter ligation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD rna preparation 5 adapter ligation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD library generation pcr product isolation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD preparation reverse transcription protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD library generation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD read strand mate 2 sense rna purity 260-230 ratio 2.13 rna integrity number 9.5 ERX8902543 SSA_ROSLIN_AF_WP1_ATAC_03_1 ERP136504 PRJEB51854 ATAC-seq genomic paired-end experiment using Illumina's IDT UDI multiplexing and sequencing on Novaseq 6000 with PE150 ERS11209599 SAMEA9835781 D1100301 ATAC-seq GENOMIC other https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_ATACseq_protocol_part2_20210527.pdf Illumina NovaSeq 6000 project FAANG secondary project AQUA-FAANG assay type ATAC-seq sample storage frozen, -70 freezer sample storage processing cryopreservation, other sampling to preparation interval 10.0 months experimental protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_ATACseq_protocol_part2_20210527.pdf extraction protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_ATACseq_protocol_part2_20210527.pdf library preparation location The University of Edinburgh, Easter Bush Campus, The Roslin Institute, Midlothian EH25 9RG library preparation location longitude -3.19917081077908 decimal degrees library preparation location latitude 55.8657492911024 decimal degrees library preparation date 2020-10-10 YYYY-MM-DD sequencing location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road sequencing location longitude 0.145393 decimal degrees sequencing location latitude 52.231532 decimal degrees sequencing date 2020-10-10 YYYY-MM-DD experiment target open_chromatin_region transposase protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_ATACseq_protocol_part2_20210527.pdf ERX8902546 SSA_ROSLIN_AF_WP1_ATAC_04_1 ERP136504 PRJEB51854 ATAC-seq genomic paired-end experiment using Illumina's IDT UDI multiplexing and sequencing on Novaseq 6000 with PE150 ERS11209598 SAMEA9835782 D1100401 ATAC-seq GENOMIC other https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_ATACseq_protocol_part2_20210527.pdf Illumina NovaSeq 6000 project FAANG secondary project AQUA-FAANG assay type ATAC-seq sample storage frozen, -70 freezer sample storage processing cryopreservation, other sampling to preparation interval 10.0 months experimental protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_ATACseq_protocol_part2_20210527.pdf extraction protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_ATACseq_protocol_part2_20210527.pdf library preparation location The University of Edinburgh, Easter Bush Campus, The Roslin Institute, Midlothian EH25 9RG library preparation location longitude -3.19917081077908 decimal degrees library preparation location latitude 55.8657492911024 decimal degrees library preparation date 2020-10-10 YYYY-MM-DD sequencing location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road sequencing location longitude 0.145393 decimal degrees sequencing location latitude 52.231532 decimal degrees sequencing date 2020-10-10 YYYY-MM-DD experiment target open_chromatin_region transposase protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_ATACseq_protocol_part2_20210527.pdf ERX8902548 SSA_ROSLIN_AF_WP1_ATAC_04_3 ERP136504 PRJEB51854 ATAC-seq genomic paired-end experiment using Illumina's IDT UDI multiplexing and sequencing on Novaseq 6000 with PE150 ERS11209605 SAMEA9835796 D1100403 ATAC-seq GENOMIC other https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_ATACseq_protocol_part2_20210527.pdf Illumina NovaSeq 6000 project FAANG secondary project AQUA-FAANG assay type ATAC-seq sample storage frozen, -70 freezer sample storage processing cryopreservation, other sampling to preparation interval 10.0 months experimental protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_ATACseq_protocol_part2_20210527.pdf extraction protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_ATACseq_protocol_part2_20210527.pdf library preparation location The University of Edinburgh, Easter Bush Campus, The Roslin Institute, Midlothian EH25 9RG library preparation location longitude -3.19917081077908 decimal degrees library preparation location latitude 55.8657492911024 decimal degrees library preparation date 2020-10-10 YYYY-MM-DD sequencing location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road sequencing location longitude 0.145393 decimal degrees sequencing location latitude 52.231532 decimal degrees sequencing date 2020-10-10 YYYY-MM-DD experiment target open_chromatin_region transposase protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_ATACseq_protocol_part2_20210527.pdf ERX8902549 SSA_ROSLIN_AF_WP1_ATAC_05_1 ERP136504 PRJEB51854 ATAC-seq genomic paired-end experiment using Illumina's IDT UDI multiplexing and sequencing on Novaseq 6000 with PE150 ERS11209601 SAMEA9835783 D1100501 ATAC-seq GENOMIC other https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_ATACseq_protocol_part2_20210527.pdf Illumina NovaSeq 6000 project FAANG secondary project AQUA-FAANG assay type ATAC-seq sample storage frozen, -70 freezer sample storage processing cryopreservation, other sampling to preparation interval 10.0 months experimental protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_ATACseq_protocol_part2_20210527.pdf extraction protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_ATACseq_protocol_part2_20210527.pdf library preparation location The University of Edinburgh, Easter Bush Campus, The Roslin Institute, Midlothian EH25 9RG library preparation location longitude -3.19917081077908 decimal degrees library preparation location latitude 55.8657492911024 decimal degrees library preparation date 2020-10-10 YYYY-MM-DD sequencing location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road sequencing location longitude 0.145393 decimal degrees sequencing location latitude 52.231532 decimal degrees sequencing date 2020-10-10 YYYY-MM-DD experiment target open_chromatin_region transposase protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_ATACseq_protocol_part2_20210527.pdf ERX8902518 SSA_ROSLIN_AF_WP1_RNA_08_3 ERP136505 PRJEB51855 Stranded PE150 RNA-Seq experiment using polyA RNA selection and seqencing on a Novaseq 6000 ERS11209604 SAMEA9835752 D1300803 RNA-Seq TRANSCRIPTOMIC other NEBNext Ultra Directional RNA Library Prep Kit for Illumina E7420 manual Illumina NovaSeq 6000 project FAANG secondary project AQUA-FAANG assay type RNA-seq of coding RNA sample storage TRIzol, frozen sample storage processing fresh sampling to preparation interval 10.0 months experimental protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_RNAseq_protocol_20210527.pdf extraction protocol https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_RNAseq_protocol_20210527.pdf library preparation location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road library preparation location longitude 0.145393 decimal degrees library preparation location latitude 52.231532 decimal degrees library preparation date 2020-10-10 YYYY-MM-DD sequencing location Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road sequencing location longitude 0.145393 decimal degrees sequencing location latitude 52.231532 decimal degrees sequencing date 2020-03-13 YYYY-MM-DD experiment target rna rna preparation 3 adapter ligation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD rna preparation 5 adapter ligation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD library generation pcr product isolation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD preparation reverse transcription protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD library generation protocol https://www.neb.com/-/media/nebus/files/manuals/manuale7420.pdf?rev=7d8e30d1085b4fb1bfb581d5344aff94&hash=C5718EF3C14CF17CB05576F831C69ACD read strand mate 2 sense rna purity 260-230 ratio 1.14 rna integrity number 8.2