ERX2234762 SUS_RI_Duroc_3_BMDM ERP104852 PRJEB23119 RNA-Seq transcriptomic paired end experiment using polyA selection on an Illumina HiSeq 2500 ERS2227138 SAMEA103887279 DUR30HR RNA-Seq TRANSCRIPTOMIC Inverse rRNA TruSeq Stranded Total RNA Sample Preparation Guide Revision E Illumina HiSeq 2500 assay type transcription profiling by high throughput sequencing sample storage TRIzol, frozen sample storage processing cryopreservation, other sampling to preparation interval 9 days experimental protocol https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedmrna/truseq-stranded-mrna-sample-prep-guide-15031047-e.pdf extraction protocol ftp://ftp.faang.ebi.ac.uk/ftp/protocols/assays/ROSLIN_SOP_Isolation_of_Total_RNA_from_Cells_With_DNase_Treatment_20161116.pdf library preparation location Edinburgh Genomics, The University of Edinburgh, EH9 3FL, Edinburgh, Scotland library preparation location longitude -3.173114 decimal degrees library preparation location latitude 55.923953 decimal degrees library preparation date 2016-07 YYYY-MM sequencing location Edinburgh Genomics, The University of Edinburgh, EH9 3FL, Edinburgh, Scotland sequencing location longitude -3.173114 decimal degrees sequencing location latitude 55.923953 decimal degrees sequencing date not applicable experiment target total RNA rna preparation 3' adapter ligation protocol https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedtotalrna/truseq-stranded-total-rna-sample-prep-guide-15031048-e.pdf rna preparation 5' adapter ligation protocol https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedtotalrna/truseq-stranded-total-rna-sample-prep-guide-15031048-e.pdf library generation pcr product isolation protocol https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedtotalrna/truseq-stranded-total-rna-sample-prep-guide-15031048-e.pdf preparation reverse transcription protocol https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedtotalrna/truseq-stranded-total-rna-sample-prep-guide-15031048-e.pdf library generation protocol https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedtotalrna/truseq-stranded-total-rna-sample-prep-guide-15031048-e.pdf read strand mate 2 sense rna purity - 260:280 ratio 2.07 rna purity - 260:230 ratio 1.17 rna integrity number 9.9 ERX2234763 SUS_RI_Duroc_3_BMDM_7h ERP104852 PRJEB23119 RNA-Seq transcriptomic paired end experiment using polyA selection on an Illumina HiSeq 2500 ERS2227140 SAMEA103887272 DUR37HR RNA-Seq TRANSCRIPTOMIC Inverse rRNA TruSeq Stranded Total RNA Sample Preparation Guide Revision E Illumina HiSeq 2500 assay type transcription profiling by high throughput sequencing sample storage TRIzol, frozen sample storage processing cryopreservation, other sampling to preparation interval 9 days experimental protocol https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedmrna/truseq-stranded-mrna-sample-prep-guide-15031047-e.pdf extraction protocol ftp://ftp.faang.ebi.ac.uk/ftp/protocols/assays/ROSLIN_SOP_Isolation_of_Total_RNA_from_Cells_With_DNase_Treatment_20161116.pdf library preparation location Edinburgh Genomics, The University of Edinburgh, EH9 3FL, Edinburgh, Scotland library preparation location longitude -2.173114 decimal degrees library preparation location latitude 56.923953 decimal degrees library preparation date 2016-07 YYYY-MM sequencing location Edinburgh Genomics, The University of Edinburgh, EH9 3FL, Edinburgh, Scotland sequencing location longitude -3.173114 decimal degrees sequencing location latitude 55.923953 decimal degrees sequencing date not applicable experiment target total RNA rna preparation 3' adapter ligation protocol https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedtotalrna/truseq-stranded-total-rna-sample-prep-guide-15031048-e.pdf rna preparation 5' adapter ligation protocol https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedtotalrna/truseq-stranded-total-rna-sample-prep-guide-15031048-e.pdf library generation pcr product isolation protocol https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedtotalrna/truseq-stranded-total-rna-sample-prep-guide-15031048-e.pdf preparation reverse transcription protocol https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedtotalrna/truseq-stranded-total-rna-sample-prep-guide-15031048-e.pdf library generation protocol https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedtotalrna/truseq-stranded-total-rna-sample-prep-guide-15031048-e.pdf read strand mate 2 sense rna purity - 260:280 ratio 2.09 rna purity - 260:230 ratio 1.83 rna integrity number 10 ERX2234764 SUS_RI_Duroc_4_BMDM ERP104852 PRJEB23119 RNA-Seq transcriptomic paired end experiment using polyA selection on an Illumina HiSeq 2500 ERS2227136 SAMEA103887275 DUR40HR RNA-Seq TRANSCRIPTOMIC Inverse rRNA TruSeq Stranded Total RNA Sample Preparation Guide Revision E Illumina HiSeq 2500 assay type transcription profiling by high throughput sequencing sample storage TRIzol, frozen sample storage processing cryopreservation, other sampling to preparation interval 2 days experimental protocol https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedmrna/truseq-stranded-mrna-sample-prep-guide-15031047-e.pdf extraction protocol ftp://ftp.faang.ebi.ac.uk/ftp/protocols/assays/ROSLIN_SOP_Isolation_of_Total_RNA_from_Cells_With_DNase_Treatment_20161116.pdf library preparation location Edinburgh Genomics, The University of Edinburgh, EH9 3FL, Edinburgh, Scotland library preparation location longitude -1.173114 decimal degrees library preparation location latitude 57.923953 decimal degrees library preparation date 2016-07 YYYY-MM sequencing location Edinburgh Genomics, The University of Edinburgh, EH9 3FL, Edinburgh, Scotland sequencing location longitude -3.173114 decimal degrees sequencing location latitude 55.923953 decimal degrees sequencing date not applicable experiment target total RNA rna preparation 3' adapter ligation protocol https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedtotalrna/truseq-stranded-total-rna-sample-prep-guide-15031048-e.pdf rna preparation 5' adapter ligation protocol https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedtotalrna/truseq-stranded-total-rna-sample-prep-guide-15031048-e.pdf library generation pcr product isolation protocol https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedtotalrna/truseq-stranded-total-rna-sample-prep-guide-15031048-e.pdf preparation reverse transcription protocol https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedtotalrna/truseq-stranded-total-rna-sample-prep-guide-15031048-e.pdf library generation protocol https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedtotalrna/truseq-stranded-total-rna-sample-prep-guide-15031048-e.pdf read strand mate 2 sense rna purity - 260:280 ratio 2.02 rna purity - 260:230 ratio 2.1 rna integrity number 10 ERX2234765 SUS_RI_Duroc_4_BMDM_7h ERP104852 PRJEB23119 RNA-Seq transcriptomic paired end experiment using polyA selection on an Illumina HiSeq 2500 ERS2227139 SAMEA103887277 DUR47HR RNA-Seq TRANSCRIPTOMIC Inverse rRNA TruSeq Stranded Total RNA Sample Preparation Guide Revision E Illumina HiSeq 2500 assay type transcription profiling by high throughput sequencing sample storage TRIzol, frozen sample storage processing cryopreservation, other sampling to preparation interval 2 days experimental protocol https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedmrna/truseq-stranded-mrna-sample-prep-guide-15031047-e.pdf extraction protocol ftp://ftp.faang.ebi.ac.uk/ftp/protocols/assays/ROSLIN_SOP_Isolation_of_Total_RNA_from_Cells_With_DNase_Treatment_20161116.pdf library preparation location Edinburgh Genomics, The University of Edinburgh, EH9 3FL, Edinburgh, Scotland library preparation location longitude -0.173114 decimal degrees library preparation location latitude 58.923953 decimal degrees library preparation date 2016-07 YYYY-MM sequencing location Edinburgh Genomics, The University of Edinburgh, EH9 3FL, Edinburgh, Scotland sequencing location longitude -3.173114 decimal degrees sequencing location latitude 55.923953 decimal degrees sequencing date not applicable experiment target total RNA rna preparation 3' adapter ligation protocol https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedtotalrna/truseq-stranded-total-rna-sample-prep-guide-15031048-e.pdf rna preparation 5' adapter ligation protocol https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedtotalrna/truseq-stranded-total-rna-sample-prep-guide-15031048-e.pdf library generation pcr product isolation protocol https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedtotalrna/truseq-stranded-total-rna-sample-prep-guide-15031048-e.pdf preparation reverse transcription protocol https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedtotalrna/truseq-stranded-total-rna-sample-prep-guide-15031048-e.pdf library generation protocol https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedtotalrna/truseq-stranded-total-rna-sample-prep-guide-15031048-e.pdf read strand mate 2 sense rna purity - 260:280 ratio 2.04 rna purity - 260:230 ratio 2.44 rna integrity number 10 ERX2234766 SUS_RI_Duroc_5_BMDM ERP104852 PRJEB23119 RNA-Seq transcriptomic paired end experiment using polyA selection on an Illumina HiSeq 2500 ERS2227141 SAMEA103887281 DUR50HR RNA-Seq TRANSCRIPTOMIC Inverse rRNA TruSeq Stranded Total RNA Sample Preparation Guide Revision E Illumina HiSeq 2500 assay type transcription profiling by high throughput sequencing sample storage TRIzol, frozen sample storage processing cryopreservation, other sampling to preparation interval 5 days experimental protocol https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedmrna/truseq-stranded-mrna-sample-prep-guide-15031047-e.pdf extraction protocol ftp://ftp.faang.ebi.ac.uk/ftp/protocols/assays/ROSLIN_SOP_Isolation_of_Total_RNA_from_Cells_With_DNase_Treatment_20161116.pdf library preparation location Edinburgh Genomics, The University of Edinburgh, EH9 3FL, Edinburgh, Scotland library preparation location longitude 0.826886 decimal degrees library preparation location latitude 59.923953 decimal degrees library preparation date 2016-07 YYYY-MM sequencing location Edinburgh Genomics, The University of Edinburgh, EH9 3FL, Edinburgh, Scotland sequencing location longitude -3.173114 decimal degrees sequencing location latitude 55.923953 decimal degrees sequencing date not applicable experiment target total RNA rna preparation 3' adapter ligation protocol https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedtotalrna/truseq-stranded-total-rna-sample-prep-guide-15031048-e.pdf rna preparation 5' adapter ligation protocol https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedtotalrna/truseq-stranded-total-rna-sample-prep-guide-15031048-e.pdf library generation pcr product isolation protocol https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedtotalrna/truseq-stranded-total-rna-sample-prep-guide-15031048-e.pdf preparation reverse transcription protocol https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedtotalrna/truseq-stranded-total-rna-sample-prep-guide-15031048-e.pdf library generation protocol https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedtotalrna/truseq-stranded-total-rna-sample-prep-guide-15031048-e.pdf read strand mate 2 sense rna purity - 260:280 ratio 2.08 rna purity - 260:230 ratio 2.31 rna integrity number 10 ERX2234767 SUS_RI_Duroc_5_BMDM_7h ERP104852 PRJEB23119 RNA-Seq transcriptomic paired end experiment using polyA selection on an Illumina HiSeq 2500 ERS2227137 SAMEA103887274 DUR57HR RNA-Seq TRANSCRIPTOMIC Inverse rRNA TruSeq Stranded Total RNA Sample Preparation Guide Revision E Illumina HiSeq 2500 assay type transcription profiling by high throughput sequencing sample storage TRIzol, frozen sample storage processing cryopreservation, other sampling to preparation interval 5 days experimental protocol https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedmrna/truseq-stranded-mrna-sample-prep-guide-15031047-e.pdf extraction protocol ftp://ftp.faang.ebi.ac.uk/ftp/protocols/assays/ROSLIN_SOP_Isolation_of_Total_RNA_from_Cells_With_DNase_Treatment_20161116.pdf library preparation location Edinburgh Genomics, The University of Edinburgh, EH9 3FL, Edinburgh, Scotland library preparation location longitude 1.826886 decimal degrees library preparation location latitude 60.923953 decimal degrees library preparation date 2016-07 YYYY-MM sequencing location Edinburgh Genomics, The University of Edinburgh, EH9 3FL, Edinburgh, Scotland sequencing location longitude -3.173114 decimal degrees sequencing location latitude 55.923953 decimal degrees sequencing date not applicable experiment target total RNA rna preparation 3' adapter ligation protocol https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedtotalrna/truseq-stranded-total-rna-sample-prep-guide-15031048-e.pdf rna preparation 5' adapter ligation protocol https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedtotalrna/truseq-stranded-total-rna-sample-prep-guide-15031048-e.pdf library generation pcr product isolation protocol https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedtotalrna/truseq-stranded-total-rna-sample-prep-guide-15031048-e.pdf preparation reverse transcription protocol https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedtotalrna/truseq-stranded-total-rna-sample-prep-guide-15031048-e.pdf library generation protocol https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedtotalrna/truseq-stranded-total-rna-sample-prep-guide-15031048-e.pdf read strand mate 2 sense rna purity - 260:280 ratio 2.08 rna purity - 260:230 ratio 2.26 rna integrity number 10