ERX2244190 E-MTAB-6122:linear_s ERP105018 PRJEB23279 Terminus DNA loss in the Escherichia coli ERS1989084 SAMEA104364106 linear_s WGS GENOMIC size fractionation Cells were grown in M9 minimal medium supplemented with 0.4 % glucose to 801 exponential phase (0.2 OD 650 nm). Study of the mechanisms of RecB mutant terminus DNA loss in Escherichia coli. FX158: WT MG1655 FX35: recB- FX37: ruvAB- FX51: matP- MIC18: recB- sbcD- sbcC- MIC20: recB- ruvAB- MIC24: matP- recB- MIC25: recA- recB- MIC31: sbcB- sbcD- MIC34: recA- recD- MIC40: linear chromosome MIC41: linear chromosome recB- MIC42: matP- ftsKC- MIC43: matP- ftsKC- recB- MIC48: recA- Cells were grown in M9 minimal medium supplemented with 0.4 % glucose to exponential phase (0.2 OD 650 nm). Chromosomal DNA was extracted using the Sigma GenElute bacterial genomic DNA kit. 5 μg of DNA were used to generate a genomic library according to Illumina's protocol. Genomic DNA Libraries were made with the 'Nextera DNA library preparation kit' (Illumina). Library quality was assessed on an Agilent Bioanalyzer 2100, using an Agilent High Sensitivity DNA Kit (Agilent technologies). NextSeq 500 Experimental Factor: genotype linear chromosome ERX2244191 E-MTAB-6122:linear recB_s ERP105018 PRJEB23279 Terminus DNA loss in the Escherichia coli ERS1989085 SAMEA104364107 linear recB_s WGS GENOMIC size fractionation Cells were grown in M9 minimal medium supplemented with 0.4 % glucose to 801 exponential phase (0.2 OD 650 nm). Study of the mechanisms of RecB mutant terminus DNA loss in Escherichia coli. FX158: WT MG1655 FX35: recB- FX37: ruvAB- FX51: matP- MIC18: recB- sbcD- sbcC- MIC20: recB- ruvAB- MIC24: matP- recB- MIC25: recA- recB- MIC31: sbcB- sbcD- MIC34: recA- recD- MIC40: linear chromosome MIC41: linear chromosome recB- MIC42: matP- ftsKC- MIC43: matP- ftsKC- recB- MIC48: recA- Cells were grown in M9 minimal medium supplemented with 0.4 % glucose to exponential phase (0.2 OD 650 nm). Chromosomal DNA was extracted using the Sigma GenElute bacterial genomic DNA kit. 5 μg of DNA were used to generate a genomic library according to Illumina's protocol. Genomic DNA Libraries were made with the 'Nextera DNA library preparation kit' (Illumina). Library quality was assessed on an Agilent Bioanalyzer 2100, using an Agilent High Sensitivity DNA Kit (Agilent technologies). NextSeq 500 Experimental Factor: genotype linear chromosome, recB ERX2244192 E-MTAB-6122:matP_s ERP105018 PRJEB23279 Terminus DNA loss in the Escherichia coli ERS1989086 SAMEA104364108 matP_s WGS GENOMIC size fractionation Cells were grown in M9 minimal medium supplemented with 0.4 % glucose to 801 exponential phase (0.2 OD 650 nm). Study of the mechanisms of RecB mutant terminus DNA loss in Escherichia coli. FX158: WT MG1655 FX35: recB- FX37: ruvAB- FX51: matP- MIC18: recB- sbcD- sbcC- MIC20: recB- ruvAB- MIC24: matP- recB- MIC25: recA- recB- MIC31: sbcB- sbcD- MIC34: recA- recD- MIC40: linear chromosome MIC41: linear chromosome recB- MIC42: matP- ftsKC- MIC43: matP- ftsKC- recB- MIC48: recA- Cells were grown in M9 minimal medium supplemented with 0.4 % glucose to exponential phase (0.2 OD 650 nm). Chromosomal DNA was extracted using the Sigma GenElute bacterial genomic DNA kit. 5 μg of DNA were used to generate a genomic library according to Illumina's protocol. Genomic DNA Libraries were made with the 'Nextera DNA library preparation kit' (Illumina). Library quality was assessed on an Agilent Bioanalyzer 2100, using an Agilent High Sensitivity DNA Kit (Agilent technologies). NextSeq 500 Experimental Factor: genotype matP ERX2244193 E-MTAB-6122:matP FtsKdc_s ERP105018 PRJEB23279 Terminus DNA loss in the Escherichia coli ERS1989087 SAMEA104364109 matP FtsKdc_s WGS GENOMIC size fractionation Cells were grown in M9 minimal medium supplemented with 0.4 % glucose to 801 exponential phase (0.2 OD 650 nm). Study of the mechanisms of RecB mutant terminus DNA loss in Escherichia coli. FX158: WT MG1655 FX35: recB- FX37: ruvAB- FX51: matP- MIC18: recB- sbcD- sbcC- MIC20: recB- ruvAB- MIC24: matP- recB- MIC25: recA- recB- MIC31: sbcB- sbcD- MIC34: recA- recD- MIC40: linear chromosome MIC41: linear chromosome recB- MIC42: matP- ftsKC- MIC43: matP- ftsKC- recB- MIC48: recA- Cells were grown in M9 minimal medium supplemented with 0.4 % glucose to exponential phase (0.2 OD 650 nm). Chromosomal DNA was extracted using the Sigma GenElute bacterial genomic DNA kit. 5 μg of DNA were used to generate a genomic library according to Illumina's protocol. Genomic DNA Libraries were made with the 'Nextera DNA library preparation kit' (Illumina). Library quality was assessed on an Agilent Bioanalyzer 2100, using an Agilent High Sensitivity DNA Kit (Agilent technologies). NextSeq 500 Experimental Factor: genotype matP FtsKCter ERX2244194 E-MTAB-6122:matP FtsKdc recB_s ERP105018 PRJEB23279 Terminus DNA loss in the Escherichia coli ERS1989088 SAMEA104364110 matP FtsKdc recB_s WGS GENOMIC size fractionation Cells were grown in M9 minimal medium supplemented with 0.4 % glucose to 801 exponential phase (0.2 OD 650 nm). Study of the mechanisms of RecB mutant terminus DNA loss in Escherichia coli. FX158: WT MG1655 FX35: recB- FX37: ruvAB- FX51: matP- MIC18: recB- sbcD- sbcC- MIC20: recB- ruvAB- MIC24: matP- recB- MIC25: recA- recB- MIC31: sbcB- sbcD- MIC34: recA- recD- MIC40: linear chromosome MIC41: linear chromosome recB- MIC42: matP- ftsKC- MIC43: matP- ftsKC- recB- MIC48: recA- Cells were grown in M9 minimal medium supplemented with 0.4 % glucose to exponential phase (0.2 OD 650 nm). Chromosomal DNA was extracted using the Sigma GenElute bacterial genomic DNA kit. 5 μg of DNA were used to generate a genomic library according to Illumina's protocol. Genomic DNA Libraries were made with the 'Nextera DNA library preparation kit' (Illumina). Library quality was assessed on an Agilent Bioanalyzer 2100, using an Agilent High Sensitivity DNA Kit (Agilent technologies). NextSeq 500 Experimental Factor: genotype matP FtsKCter recB ERX2244195 E-MTAB-6122:matP recB_s ERP105018 PRJEB23279 Terminus DNA loss in the Escherichia coli ERS1989089 SAMEA104364111 matP recB_s WGS GENOMIC size fractionation Cells were grown in M9 minimal medium supplemented with 0.4 % glucose to 801 exponential phase (0.2 OD 650 nm). Study of the mechanisms of RecB mutant terminus DNA loss in Escherichia coli. FX158: WT MG1655 FX35: recB- FX37: ruvAB- FX51: matP- MIC18: recB- sbcD- sbcC- MIC20: recB- ruvAB- MIC24: matP- recB- MIC25: recA- recB- MIC31: sbcB- sbcD- MIC34: recA- recD- MIC40: linear chromosome MIC41: linear chromosome recB- MIC42: matP- ftsKC- MIC43: matP- ftsKC- recB- MIC48: recA- Cells were grown in M9 minimal medium supplemented with 0.4 % glucose to exponential phase (0.2 OD 650 nm). Chromosomal DNA was extracted using the Sigma GenElute bacterial genomic DNA kit. 5 μg of DNA were used to generate a genomic library according to Illumina's protocol. Genomic DNA Libraries were made with the 'Nextera DNA library preparation kit' (Illumina). Library quality was assessed on an Agilent Bioanalyzer 2100, using an Agilent High Sensitivity DNA Kit (Agilent technologies). NextSeq 500 Experimental Factor: genotype matP recB ERX2244196 E-MTAB-6122:recA_s ERP105018 PRJEB23279 Terminus DNA loss in the Escherichia coli ERS1989090 SAMEA104364112 recA_s WGS GENOMIC size fractionation Cells were grown in M9 minimal medium supplemented with 0.4 % glucose to 801 exponential phase (0.2 OD 650 nm). Study of the mechanisms of RecB mutant terminus DNA loss in Escherichia coli. FX158: WT MG1655 FX35: recB- FX37: ruvAB- FX51: matP- MIC18: recB- sbcD- sbcC- MIC20: recB- ruvAB- MIC24: matP- recB- MIC25: recA- recB- MIC31: sbcB- sbcD- MIC34: recA- recD- MIC40: linear chromosome MIC41: linear chromosome recB- MIC42: matP- ftsKC- MIC43: matP- ftsKC- recB- MIC48: recA- Cells were grown in M9 minimal medium supplemented with 0.4 % glucose to exponential phase (0.2 OD 650 nm). Chromosomal DNA was extracted using the Sigma GenElute bacterial genomic DNA kit. 5 μg of DNA were used to generate a genomic library according to Illumina's protocol. Genomic DNA Libraries were made with the 'Nextera DNA library preparation kit' (Illumina). Library quality was assessed on an Agilent Bioanalyzer 2100, using an Agilent High Sensitivity DNA Kit (Agilent technologies). NextSeq 500 Experimental Factor: genotype recA ERX2244197 E-MTAB-6122:recA recB_s ERP105018 PRJEB23279 Terminus DNA loss in the Escherichia coli ERS1989091 SAMEA104364113 recA recB_s WGS GENOMIC size fractionation Cells were grown in M9 minimal medium supplemented with 0.4 % glucose to 801 exponential phase (0.2 OD 650 nm). Study of the mechanisms of RecB mutant terminus DNA loss in Escherichia coli. FX158: WT MG1655 FX35: recB- FX37: ruvAB- FX51: matP- MIC18: recB- sbcD- sbcC- MIC20: recB- ruvAB- MIC24: matP- recB- MIC25: recA- recB- MIC31: sbcB- sbcD- MIC34: recA- recD- MIC40: linear chromosome MIC41: linear chromosome recB- MIC42: matP- ftsKC- MIC43: matP- ftsKC- recB- MIC48: recA- Cells were grown in M9 minimal medium supplemented with 0.4 % glucose to exponential phase (0.2 OD 650 nm). Chromosomal DNA was extracted using the Sigma GenElute bacterial genomic DNA kit. 5 μg of DNA were used to generate a genomic library according to Illumina's protocol. Genomic DNA Libraries were made with the 'Nextera DNA library preparation kit' (Illumina). Library quality was assessed on an Agilent Bioanalyzer 2100, using an Agilent High Sensitivity DNA Kit (Agilent technologies). NextSeq 500 Experimental Factor: genotype recA recB ERX2244198 E-MTAB-6122:recA recD_s ERP105018 PRJEB23279 Terminus DNA loss in the Escherichia coli ERS1989092 SAMEA104364114 recA recD_s WGS GENOMIC size fractionation Cells were grown in M9 minimal medium supplemented with 0.4 % glucose to 801 exponential phase (0.2 OD 650 nm). Study of the mechanisms of RecB mutant terminus DNA loss in Escherichia coli. FX158: WT MG1655 FX35: recB- FX37: ruvAB- FX51: matP- MIC18: recB- sbcD- sbcC- MIC20: recB- ruvAB- MIC24: matP- recB- MIC25: recA- recB- MIC31: sbcB- sbcD- MIC34: recA- recD- MIC40: linear chromosome MIC41: linear chromosome recB- MIC42: matP- ftsKC- MIC43: matP- ftsKC- recB- MIC48: recA- Cells were grown in M9 minimal medium supplemented with 0.4 % glucose to exponential phase (0.2 OD 650 nm). Chromosomal DNA was extracted using the Sigma GenElute bacterial genomic DNA kit. 5 μg of DNA were used to generate a genomic library according to Illumina's protocol. Genomic DNA Libraries were made with the 'Nextera DNA library preparation kit' (Illumina). Library quality was assessed on an Agilent Bioanalyzer 2100, using an Agilent High Sensitivity DNA Kit (Agilent technologies). NextSeq 500 Experimental Factor: genotype recA recD ERX2244199 E-MTAB-6122:recB_s ERP105018 PRJEB23279 Terminus DNA loss in the Escherichia coli ERS1989093 SAMEA104364115 recB_s WGS GENOMIC size fractionation Cells were grown in M9 minimal medium supplemented with 0.4 % glucose to 801 exponential phase (0.2 OD 650 nm). Study of the mechanisms of RecB mutant terminus DNA loss in Escherichia coli. FX158: WT MG1655 FX35: recB- FX37: ruvAB- FX51: matP- MIC18: recB- sbcD- sbcC- MIC20: recB- ruvAB- MIC24: matP- recB- MIC25: recA- recB- MIC31: sbcB- sbcD- MIC34: recA- recD- MIC40: linear chromosome MIC41: linear chromosome recB- MIC42: matP- ftsKC- MIC43: matP- ftsKC- recB- MIC48: recA- Cells were grown in M9 minimal medium supplemented with 0.4 % glucose to exponential phase (0.2 OD 650 nm). Chromosomal DNA was extracted using the Sigma GenElute bacterial genomic DNA kit. 5 μg of DNA were used to generate a genomic library according to Illumina's protocol. Genomic DNA Libraries were made with the 'Nextera DNA library preparation kit' (Illumina). Library quality was assessed on an Agilent Bioanalyzer 2100, using an Agilent High Sensitivity DNA Kit (Agilent technologies). NextSeq 500 Experimental Factor: genotype recB ERX2244200 E-MTAB-6122:ruvAB_s ERP105018 PRJEB23279 Terminus DNA loss in the Escherichia coli ERS1989094 SAMEA104364116 ruvAB_s WGS GENOMIC size fractionation Cells were grown in M9 minimal medium supplemented with 0.4 % glucose to 801 exponential phase (0.2 OD 650 nm). Study of the mechanisms of RecB mutant terminus DNA loss in Escherichia coli. FX158: WT MG1655 FX35: recB- FX37: ruvAB- FX51: matP- MIC18: recB- sbcD- sbcC- MIC20: recB- ruvAB- MIC24: matP- recB- MIC25: recA- recB- MIC31: sbcB- sbcD- MIC34: recA- recD- MIC40: linear chromosome MIC41: linear chromosome recB- MIC42: matP- ftsKC- MIC43: matP- ftsKC- recB- MIC48: recA- Cells were grown in M9 minimal medium supplemented with 0.4 % glucose to exponential phase (0.2 OD 650 nm). Chromosomal DNA was extracted using the Sigma GenElute bacterial genomic DNA kit. 5 μg of DNA were used to generate a genomic library according to Illumina's protocol. Genomic DNA Libraries were made with the 'Nextera DNA library preparation kit' (Illumina). Library quality was assessed on an Agilent Bioanalyzer 2100, using an Agilent High Sensitivity DNA Kit (Agilent technologies). NextSeq 500 Experimental Factor: genotype ruvA ruvB ERX2244201 E-MTAB-6122:ruvAB recB_s ERP105018 PRJEB23279 Terminus DNA loss in the Escherichia coli ERS1989095 SAMEA104364117 ruvAB recB_s WGS GENOMIC size fractionation Cells were grown in M9 minimal medium supplemented with 0.4 % glucose to 801 exponential phase (0.2 OD 650 nm). Study of the mechanisms of RecB mutant terminus DNA loss in Escherichia coli. FX158: WT MG1655 FX35: recB- FX37: ruvAB- FX51: matP- MIC18: recB- sbcD- sbcC- MIC20: recB- ruvAB- MIC24: matP- recB- MIC25: recA- recB- MIC31: sbcB- sbcD- MIC34: recA- recD- MIC40: linear chromosome MIC41: linear chromosome recB- MIC42: matP- ftsKC- MIC43: matP- ftsKC- recB- MIC48: recA- Cells were grown in M9 minimal medium supplemented with 0.4 % glucose to exponential phase (0.2 OD 650 nm). Chromosomal DNA was extracted using the Sigma GenElute bacterial genomic DNA kit. 5 μg of DNA were used to generate a genomic library according to Illumina's protocol. Genomic DNA Libraries were made with the 'Nextera DNA library preparation kit' (Illumina). Library quality was assessed on an Agilent Bioanalyzer 2100, using an Agilent High Sensitivity DNA Kit (Agilent technologies). NextSeq 500 Experimental Factor: genotype ruvA ruvB recB ERX2244202 E-MTAB-6122:sbcB sbcD_s ERP105018 PRJEB23279 Terminus DNA loss in the Escherichia coli ERS1989096 SAMEA104364118 sbcB sbcD_s WGS GENOMIC size fractionation Cells were grown in M9 minimal medium supplemented with 0.4 % glucose to 801 exponential phase (0.2 OD 650 nm). Study of the mechanisms of RecB mutant terminus DNA loss in Escherichia coli. FX158: WT MG1655 FX35: recB- FX37: ruvAB- FX51: matP- MIC18: recB- sbcD- sbcC- MIC20: recB- ruvAB- MIC24: matP- recB- MIC25: recA- recB- MIC31: sbcB- sbcD- MIC34: recA- recD- MIC40: linear chromosome MIC41: linear chromosome recB- MIC42: matP- ftsKC- MIC43: matP- ftsKC- recB- MIC48: recA- Cells were grown in M9 minimal medium supplemented with 0.4 % glucose to exponential phase (0.2 OD 650 nm). Chromosomal DNA was extracted using the Sigma GenElute bacterial genomic DNA kit. 5 μg of DNA were used to generate a genomic library according to Illumina's protocol. Genomic DNA Libraries were made with the 'Nextera DNA library preparation kit' (Illumina). Library quality was assessed on an Agilent Bioanalyzer 2100, using an Agilent High Sensitivity DNA Kit (Agilent technologies). NextSeq 500 Experimental Factor: genotype sbcB sbcD ERX2244203 E-MTAB-6122:sbcB sbcD recB_s ERP105018 PRJEB23279 Terminus DNA loss in the Escherichia coli ERS1989097 SAMEA104364119 sbcB sbcD recB_s WGS GENOMIC size fractionation Cells were grown in M9 minimal medium supplemented with 0.4 % glucose to 801 exponential phase (0.2 OD 650 nm). Study of the mechanisms of RecB mutant terminus DNA loss in Escherichia coli. FX158: WT MG1655 FX35: recB- FX37: ruvAB- FX51: matP- MIC18: recB- sbcD- sbcC- MIC20: recB- ruvAB- MIC24: matP- recB- MIC25: recA- recB- MIC31: sbcB- sbcD- MIC34: recA- recD- MIC40: linear chromosome MIC41: linear chromosome recB- MIC42: matP- ftsKC- MIC43: matP- ftsKC- recB- MIC48: recA- Cells were grown in M9 minimal medium supplemented with 0.4 % glucose to exponential phase (0.2 OD 650 nm). Chromosomal DNA was extracted using the Sigma GenElute bacterial genomic DNA kit. 5 μg of DNA were used to generate a genomic library according to Illumina's protocol. Genomic DNA Libraries were made with the 'Nextera DNA library preparation kit' (Illumina). Library quality was assessed on an Agilent Bioanalyzer 2100, using an Agilent High Sensitivity DNA Kit (Agilent technologies). NextSeq 500 Experimental Factor: genotype sbcB sbcD recB ERX2244204 E-MTAB-6122:wt_s ERP105018 PRJEB23279 Terminus DNA loss in the Escherichia coli ERS1989098 SAMEA104364120 wt_s WGS GENOMIC size fractionation Cells were grown in M9 minimal medium supplemented with 0.4 % glucose to 801 exponential phase (0.2 OD 650 nm). Study of the mechanisms of RecB mutant terminus DNA loss in Escherichia coli. FX158: WT MG1655 FX35: recB- FX37: ruvAB- FX51: matP- MIC18: recB- sbcD- sbcC- MIC20: recB- ruvAB- MIC24: matP- recB- MIC25: recA- recB- MIC31: sbcB- sbcD- MIC34: recA- recD- MIC40: linear chromosome MIC41: linear chromosome recB- MIC42: matP- ftsKC- MIC43: matP- ftsKC- recB- MIC48: recA- Cells were grown in M9 minimal medium supplemented with 0.4 % glucose to exponential phase (0.2 OD 650 nm). Chromosomal DNA was extracted using the Sigma GenElute bacterial genomic DNA kit. 5 μg of DNA were used to generate a genomic library according to Illumina's protocol. Genomic DNA Libraries were made with the 'Nextera DNA library preparation kit' (Illumina). Library quality was assessed on an Agilent Bioanalyzer 2100, using an Agilent High Sensitivity DNA Kit (Agilent technologies). NextSeq 500 Experimental Factor: genotype wild type genotype