ERX2249183 E-MTAB-3007_2:A17 LCO WT001_p ERP105151 PRJEB23399 CK dependent Rhizobium LCO induced signalling in Medicago truncatula ERS2000956 SAMEA104375978 A17 LCO WT001_p RNA-Seq TRANSCRIPTOMIC unspecified Medicago truncatula (cv. Jemalong A17) wild-type and Mtcre1-1 mutant (Plet et al., 2011: Plant Journal 65:622-633) seedlings were grown vertically for three days on modified Fåhraeus medium (Fåhraeus, 1957: Journal of General Microbiology 16:374-381) agar plates with low nitrate (including 0.2 mM Ca(NO3)2) supplemented with 1 μM amino ethoxyvinylglycine (AVG). Sinorhizobium meliloti 2011 Nod factors (~10-9 M) or water control was pipetted on top of the root susceptible zone. Roots were exposed for three hours and subsequently 1 cm root pieces were cut just above the root tip and snap-frozen in liquid nitrogen (~15 zones per replicate). For all experiments plants were grown in an environmentally controlled growth chamber at 20°C with a 16h-light/8h-dark cycle and 70% relative humidity. Illumina Truseq (Transcriptome) 180 0 F Application Read Forward 1 1 R Application Read Reverse 91 Illumina HiSeq 2000 Experimental Factor: genotype wild type genotype Experimental Factor: stimulus Rhizobium lipo-chitin oligosaccharide ERX2249184 E-MTAB-3007_2:A17 LCO WT002_p ERP105151 PRJEB23399 CK dependent Rhizobium LCO induced signalling in Medicago truncatula ERS2000957 SAMEA104375979 A17 LCO WT002_p RNA-Seq TRANSCRIPTOMIC unspecified Medicago truncatula (cv. Jemalong A17) wild-type and Mtcre1-1 mutant (Plet et al., 2011: Plant Journal 65:622-633) seedlings were grown vertically for three days on modified Fåhraeus medium (Fåhraeus, 1957: Journal of General Microbiology 16:374-381) agar plates with low nitrate (including 0.2 mM Ca(NO3)2) supplemented with 1 μM amino ethoxyvinylglycine (AVG). Sinorhizobium meliloti 2011 Nod factors (~10-9 M) or water control was pipetted on top of the root susceptible zone. Roots were exposed for three hours and subsequently 1 cm root pieces were cut just above the root tip and snap-frozen in liquid nitrogen (~15 zones per replicate). For all experiments plants were grown in an environmentally controlled growth chamber at 20°C with a 16h-light/8h-dark cycle and 70% relative humidity. Illumina Truseq (Transcriptome) 180 0 F Application Read Forward 1 1 R Application Read Reverse 91 Illumina HiSeq 2000 Experimental Factor: genotype wild type genotype Experimental Factor: stimulus Rhizobium lipo-chitin oligosaccharide ERX2249185 E-MTAB-3007_2:A17 LCO WT003_p ERP105151 PRJEB23399 CK dependent Rhizobium LCO induced signalling in Medicago truncatula ERS2000958 SAMEA104375980 A17 LCO WT003_p RNA-Seq TRANSCRIPTOMIC unspecified Medicago truncatula (cv. Jemalong A17) wild-type and Mtcre1-1 mutant (Plet et al., 2011: Plant Journal 65:622-633) seedlings were grown vertically for three days on modified Fåhraeus medium (Fåhraeus, 1957: Journal of General Microbiology 16:374-381) agar plates with low nitrate (including 0.2 mM Ca(NO3)2) supplemented with 1 μM amino ethoxyvinylglycine (AVG). Sinorhizobium meliloti 2011 Nod factors (~10-9 M) or water control was pipetted on top of the root susceptible zone. Roots were exposed for three hours and subsequently 1 cm root pieces were cut just above the root tip and snap-frozen in liquid nitrogen (~15 zones per replicate). For all experiments plants were grown in an environmentally controlled growth chamber at 20°C with a 16h-light/8h-dark cycle and 70% relative humidity. Illumina Truseq (Transcriptome) 180 0 F Application Read Forward 1 1 R Application Read Reverse 91 Illumina HiSeq 2000 Experimental Factor: genotype wild type genotype Experimental Factor: stimulus Rhizobium lipo-chitin oligosaccharide ERX2249186 E-MTAB-3007_2:A17 noLCO WT001_p ERP105151 PRJEB23399 CK dependent Rhizobium LCO induced signalling in Medicago truncatula ERS2000959 SAMEA104375981 A17 noLCO WT001_p RNA-Seq TRANSCRIPTOMIC unspecified Medicago truncatula (cv. Jemalong A17) wild-type and Mtcre1-1 mutant (Plet et al., 2011: Plant Journal 65:622-633) seedlings were grown vertically for three days on modified Fåhraeus medium (Fåhraeus, 1957: Journal of General Microbiology 16:374-381) agar plates with low nitrate (including 0.2 mM Ca(NO3)2) supplemented with 1 μM amino ethoxyvinylglycine (AVG). Sinorhizobium meliloti 2011 Nod factors (~10-9 M) or water control was pipetted on top of the root susceptible zone. Roots were exposed for three hours and subsequently 1 cm root pieces were cut just above the root tip and snap-frozen in liquid nitrogen (~15 zones per replicate). For all experiments plants were grown in an environmentally controlled growth chamber at 20°C with a 16h-light/8h-dark cycle and 70% relative humidity. Illumina Truseq (Transcriptome) 180 0 F Application Read Forward 1 1 R Application Read Reverse 91 Illumina HiSeq 2000 Experimental Factor: genotype wild type genotype Experimental Factor: stimulus none ERX2249187 E-MTAB-3007_2:A17 noLCO WT002_p ERP105151 PRJEB23399 CK dependent Rhizobium LCO induced signalling in Medicago truncatula ERS2000960 SAMEA104375982 A17 noLCO WT002_p RNA-Seq TRANSCRIPTOMIC unspecified Medicago truncatula (cv. Jemalong A17) wild-type and Mtcre1-1 mutant (Plet et al., 2011: Plant Journal 65:622-633) seedlings were grown vertically for three days on modified Fåhraeus medium (Fåhraeus, 1957: Journal of General Microbiology 16:374-381) agar plates with low nitrate (including 0.2 mM Ca(NO3)2) supplemented with 1 μM amino ethoxyvinylglycine (AVG). Sinorhizobium meliloti 2011 Nod factors (~10-9 M) or water control was pipetted on top of the root susceptible zone. Roots were exposed for three hours and subsequently 1 cm root pieces were cut just above the root tip and snap-frozen in liquid nitrogen (~15 zones per replicate). For all experiments plants were grown in an environmentally controlled growth chamber at 20°C with a 16h-light/8h-dark cycle and 70% relative humidity. Illumina Truseq (Transcriptome) 180 0 F Application Read Forward 1 1 R Application Read Reverse 91 Illumina HiSeq 2000 Experimental Factor: genotype wild type genotype Experimental Factor: stimulus none ERX2249188 E-MTAB-3007_2:A17 noLCO WT003_p ERP105151 PRJEB23399 CK dependent Rhizobium LCO induced signalling in Medicago truncatula ERS2000961 SAMEA104375983 A17 noLCO WT003_p RNA-Seq TRANSCRIPTOMIC unspecified Medicago truncatula (cv. Jemalong A17) wild-type and Mtcre1-1 mutant (Plet et al., 2011: Plant Journal 65:622-633) seedlings were grown vertically for three days on modified Fåhraeus medium (Fåhraeus, 1957: Journal of General Microbiology 16:374-381) agar plates with low nitrate (including 0.2 mM Ca(NO3)2) supplemented with 1 μM amino ethoxyvinylglycine (AVG). Sinorhizobium meliloti 2011 Nod factors (~10-9 M) or water control was pipetted on top of the root susceptible zone. Roots were exposed for three hours and subsequently 1 cm root pieces were cut just above the root tip and snap-frozen in liquid nitrogen (~15 zones per replicate). For all experiments plants were grown in an environmentally controlled growth chamber at 20°C with a 16h-light/8h-dark cycle and 70% relative humidity. Illumina Truseq (Transcriptome) 180 0 F Application Read Forward 1 1 R Application Read Reverse 91 Illumina HiSeq 2000 Experimental Factor: genotype wild type genotype Experimental Factor: stimulus none ERX2249189 E-MTAB-3007_2:Mtcre1 LCO WT001_p ERP105151 PRJEB23399 CK dependent Rhizobium LCO induced signalling in Medicago truncatula ERS2000962 SAMEA104375984 Mtcre1 LCO WT001_p RNA-Seq TRANSCRIPTOMIC unspecified Medicago truncatula (cv. Jemalong A17) wild-type and Mtcre1-1 mutant (Plet et al., 2011: Plant Journal 65:622-633) seedlings were grown vertically for three days on modified Fåhraeus medium (Fåhraeus, 1957: Journal of General Microbiology 16:374-381) agar plates with low nitrate (including 0.2 mM Ca(NO3)2) supplemented with 1 μM amino ethoxyvinylglycine (AVG). Sinorhizobium meliloti 2011 Nod factors (~10-9 M) or water control was pipetted on top of the root susceptible zone. Roots were exposed for three hours and subsequently 1 cm root pieces were cut just above the root tip and snap-frozen in liquid nitrogen (~15 zones per replicate). For all experiments plants were grown in an environmentally controlled growth chamber at 20°C with a 16h-light/8h-dark cycle and 70% relative humidity. Illumina Truseq (Transcriptome) 180 0 F Application Read Forward 1 1 R Application Read Reverse 91 Illumina HiSeq 2000 Experimental Factor: genotype Mtcre1 mutant Experimental Factor: stimulus Rhizobium lipo-chitin oligosaccharide ERX2249190 E-MTAB-3007_2:Mtcre1 LCO WT002_p ERP105151 PRJEB23399 CK dependent Rhizobium LCO induced signalling in Medicago truncatula ERS2000963 SAMEA104375985 Mtcre1 LCO WT002_p RNA-Seq TRANSCRIPTOMIC unspecified Medicago truncatula (cv. Jemalong A17) wild-type and Mtcre1-1 mutant (Plet et al., 2011: Plant Journal 65:622-633) seedlings were grown vertically for three days on modified Fåhraeus medium (Fåhraeus, 1957: Journal of General Microbiology 16:374-381) agar plates with low nitrate (including 0.2 mM Ca(NO3)2) supplemented with 1 μM amino ethoxyvinylglycine (AVG). Sinorhizobium meliloti 2011 Nod factors (~10-9 M) or water control was pipetted on top of the root susceptible zone. Roots were exposed for three hours and subsequently 1 cm root pieces were cut just above the root tip and snap-frozen in liquid nitrogen (~15 zones per replicate). For all experiments plants were grown in an environmentally controlled growth chamber at 20°C with a 16h-light/8h-dark cycle and 70% relative humidity. Illumina Truseq (Transcriptome) 180 0 F Application Read Forward 1 1 R Application Read Reverse 91 Illumina HiSeq 2000 Experimental Factor: genotype Mtcre1 mutant Experimental Factor: stimulus Rhizobium lipo-chitin oligosaccharide ERX2249191 E-MTAB-3007_2:Mtcre1 LCO WT003_p ERP105151 PRJEB23399 CK dependent Rhizobium LCO induced signalling in Medicago truncatula ERS2000964 SAMEA104375986 Mtcre1 LCO WT003_p RNA-Seq TRANSCRIPTOMIC unspecified Medicago truncatula (cv. Jemalong A17) wild-type and Mtcre1-1 mutant (Plet et al., 2011: Plant Journal 65:622-633) seedlings were grown vertically for three days on modified Fåhraeus medium (Fåhraeus, 1957: Journal of General Microbiology 16:374-381) agar plates with low nitrate (including 0.2 mM Ca(NO3)2) supplemented with 1 μM amino ethoxyvinylglycine (AVG). Sinorhizobium meliloti 2011 Nod factors (~10-9 M) or water control was pipetted on top of the root susceptible zone. Roots were exposed for three hours and subsequently 1 cm root pieces were cut just above the root tip and snap-frozen in liquid nitrogen (~15 zones per replicate). For all experiments plants were grown in an environmentally controlled growth chamber at 20°C with a 16h-light/8h-dark cycle and 70% relative humidity. Illumina Truseq (Transcriptome) 180 0 F Application Read Forward 1 1 R Application Read Reverse 91 Illumina HiSeq 2000 Experimental Factor: genotype Mtcre1 mutant Experimental Factor: stimulus Rhizobium lipo-chitin oligosaccharide ERX2249192 E-MTAB-3007_2:Mtcre1 noLCO WT001_p ERP105151 PRJEB23399 CK dependent Rhizobium LCO induced signalling in Medicago truncatula ERS2000965 SAMEA104375987 Mtcre1 noLCO WT001_p RNA-Seq TRANSCRIPTOMIC unspecified Medicago truncatula (cv. Jemalong A17) wild-type and Mtcre1-1 mutant (Plet et al., 2011: Plant Journal 65:622-633) seedlings were grown vertically for three days on modified Fåhraeus medium (Fåhraeus, 1957: Journal of General Microbiology 16:374-381) agar plates with low nitrate (including 0.2 mM Ca(NO3)2) supplemented with 1 μM amino ethoxyvinylglycine (AVG). Sinorhizobium meliloti 2011 Nod factors (~10-9 M) or water control was pipetted on top of the root susceptible zone. Roots were exposed for three hours and subsequently 1 cm root pieces were cut just above the root tip and snap-frozen in liquid nitrogen (~15 zones per replicate). For all experiments plants were grown in an environmentally controlled growth chamber at 20°C with a 16h-light/8h-dark cycle and 70% relative humidity. Illumina Truseq (Transcriptome) 180 0 F Application Read Forward 1 1 R Application Read Reverse 91 Illumina HiSeq 2000 Experimental Factor: genotype Mtcre1 mutant Experimental Factor: stimulus none ERX2249193 E-MTAB-3007_2:Mtcre1 noLCO WT002_p ERP105151 PRJEB23399 CK dependent Rhizobium LCO induced signalling in Medicago truncatula ERS2000966 SAMEA104375988 Mtcre1 noLCO WT002_p RNA-Seq TRANSCRIPTOMIC unspecified Medicago truncatula (cv. Jemalong A17) wild-type and Mtcre1-1 mutant (Plet et al., 2011: Plant Journal 65:622-633) seedlings were grown vertically for three days on modified Fåhraeus medium (Fåhraeus, 1957: Journal of General Microbiology 16:374-381) agar plates with low nitrate (including 0.2 mM Ca(NO3)2) supplemented with 1 μM amino ethoxyvinylglycine (AVG). Sinorhizobium meliloti 2011 Nod factors (~10-9 M) or water control was pipetted on top of the root susceptible zone. Roots were exposed for three hours and subsequently 1 cm root pieces were cut just above the root tip and snap-frozen in liquid nitrogen (~15 zones per replicate). For all experiments plants were grown in an environmentally controlled growth chamber at 20°C with a 16h-light/8h-dark cycle and 70% relative humidity. Illumina Truseq (Transcriptome) 180 0 F Application Read Forward 1 1 R Application Read Reverse 91 Illumina HiSeq 2000 Experimental Factor: genotype Mtcre1 mutant Experimental Factor: stimulus none ERX2249194 E-MTAB-3007_2:Mtcre1 noLCO WT003_p ERP105151 PRJEB23399 CK dependent Rhizobium LCO induced signalling in Medicago truncatula ERS2000967 SAMEA104375989 Mtcre1 noLCO WT003_p RNA-Seq TRANSCRIPTOMIC unspecified Medicago truncatula (cv. Jemalong A17) wild-type and Mtcre1-1 mutant (Plet et al., 2011: Plant Journal 65:622-633) seedlings were grown vertically for three days on modified Fåhraeus medium (Fåhraeus, 1957: Journal of General Microbiology 16:374-381) agar plates with low nitrate (including 0.2 mM Ca(NO3)2) supplemented with 1 μM amino ethoxyvinylglycine (AVG). Sinorhizobium meliloti 2011 Nod factors (~10-9 M) or water control was pipetted on top of the root susceptible zone. Roots were exposed for three hours and subsequently 1 cm root pieces were cut just above the root tip and snap-frozen in liquid nitrogen (~15 zones per replicate). For all experiments plants were grown in an environmentally controlled growth chamber at 20°C with a 16h-light/8h-dark cycle and 70% relative humidity. Illumina Truseq (Transcriptome) 180 0 F Application Read Forward 1 1 R Application Read Reverse 91 Illumina HiSeq 2000 Experimental Factor: genotype Mtcre1 mutant Experimental Factor: stimulus none