ERS2064609 SAMEA104446667 9606 Homo sapiens Protocols: CD14+CD16-HLA-DRhiLin- (CD3, CD4, CD19, CD45RO) monocytes were sorted from frozen PBMC form blood samples from HD (healthy donor), R (responder) and NR (non-responder) at baseline. Representative samples (n=4, each) of responders/non responders/ and healthy donors were selected from archival samples stored in the dermatology biobank according to the same clinical criteria used in the discovery and validation cohorts for CyTOF and FACS analysis. RNA was isolated using the RNA plus micro kit (Qiagen). We used the SMARTer Stranded total rna-seq pico kit (Clontech) for RNA preparations. Libraries for sequencing were generated with the TruSeq RNA Sample Preparation kit (Illumina). ENA first public 2017-12-21 ENA last update 2017-12-21 External Id SAMEA104446667 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2017-12-21T17:03:29Z INSDC last update 2017-12-21T11:25:49Z INSDC status public Submitter Id E-MTAB-6214:Sample 10 broker name ArrayExpress cell type mononuclear cell common name human disease metastatic melanoma organism part blood phenotype CD14+CD16-HLA-DRhiLin- response to treatment non-responder sample name E-MTAB-6214:Sample 10 treatment anti-PD-1 immunotherapy ERS2064610 SAMEA104446668 9606 Homo sapiens Protocols: CD14+CD16-HLA-DRhiLin- (CD3, CD4, CD19, CD45RO) monocytes were sorted from frozen PBMC form blood samples from HD (healthy donor), R (responder) and NR (non-responder) at baseline. Representative samples (n=4, each) of responders/non responders/ and healthy donors were selected from archival samples stored in the dermatology biobank according to the same clinical criteria used in the discovery and validation cohorts for CyTOF and FACS analysis. RNA was isolated using the RNA plus micro kit (Qiagen). We used the SMARTer Stranded total rna-seq pico kit (Clontech) for RNA preparations. Libraries for sequencing were generated with the TruSeq RNA Sample Preparation kit (Illumina). ENA first public 2017-12-21 ENA last update 2017-12-21 External Id SAMEA104446668 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2017-12-21T17:03:29Z INSDC last update 2017-12-21T11:25:49Z INSDC status public Submitter Id E-MTAB-6214:Sample 11 broker name ArrayExpress cell type mononuclear cell common name human disease metastatic melanoma organism part blood phenotype CD14+CD16-HLA-DRhiLin- response to treatment non-responder sample name E-MTAB-6214:Sample 11 treatment anti-PD-1 immunotherapy ERS2064611 SAMEA104446669 9606 Homo sapiens Protocols: CD14+CD16-HLA-DRhiLin- (CD3, CD4, CD19, CD45RO) monocytes were sorted from frozen PBMC form blood samples from HD (healthy donor), R (responder) and NR (non-responder) at baseline. Representative samples (n=4, each) of responders/non responders/ and healthy donors were selected from archival samples stored in the dermatology biobank according to the same clinical criteria used in the discovery and validation cohorts for CyTOF and FACS analysis. RNA was isolated using the RNA plus micro kit (Qiagen). We used the SMARTer Stranded total rna-seq pico kit (Clontech) for RNA preparations. Libraries for sequencing were generated with the TruSeq RNA Sample Preparation kit (Illumina). ENA first public 2017-12-21 ENA last update 2017-12-21 External Id SAMEA104446669 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2017-12-21T17:03:29Z INSDC last update 2017-12-21T11:25:49Z INSDC status public Submitter Id E-MTAB-6214:Sample 12 broker name ArrayExpress cell type mononuclear cell common name human disease metastatic melanoma organism part blood phenotype CD14+CD16-HLA-DRhiLin- response to treatment non-responder sample name E-MTAB-6214:Sample 12 treatment anti-PD-1 immunotherapy ERS2064612 SAMEA104446670 9606 Homo sapiens Protocols: CD14+CD16-HLA-DRhiLin- (CD3, CD4, CD19, CD45RO) monocytes were sorted from frozen PBMC form blood samples from HD (healthy donor), R (responder) and NR (non-responder) at baseline. Representative samples (n=4, each) of responders/non responders/ and healthy donors were selected from archival samples stored in the dermatology biobank according to the same clinical criteria used in the discovery and validation cohorts for CyTOF and FACS analysis. RNA was isolated using the RNA plus micro kit (Qiagen). We used the SMARTer Stranded total rna-seq pico kit (Clontech) for RNA preparations. Libraries for sequencing were generated with the TruSeq RNA Sample Preparation kit (Illumina). ENA first public 2017-12-21 ENA last update 2017-12-21 External Id SAMEA104446670 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2017-12-21T17:03:29Z INSDC last update 2017-12-21T11:25:49Z INSDC status public Submitter Id E-MTAB-6214:Sample 5 broker name ArrayExpress cell type mononuclear cell common name human disease metastatic melanoma organism part blood phenotype CD14+CD16-HLA-DRhiLin- response to treatment responder sample name E-MTAB-6214:Sample 5 treatment anti-PD-1 immunotherapy ERS2064613 SAMEA104446671 9606 Homo sapiens Protocols: CD14+CD16-HLA-DRhiLin- (CD3, CD4, CD19, CD45RO) monocytes were sorted from frozen PBMC form blood samples from HD (healthy donor), R (responder) and NR (non-responder) at baseline. Representative samples (n=4, each) of responders/non responders/ and healthy donors were selected from archival samples stored in the dermatology biobank according to the same clinical criteria used in the discovery and validation cohorts for CyTOF and FACS analysis. RNA was isolated using the RNA plus micro kit (Qiagen). We used the SMARTer Stranded total rna-seq pico kit (Clontech) for RNA preparations. Libraries for sequencing were generated with the TruSeq RNA Sample Preparation kit (Illumina). ENA first public 2017-12-21 ENA last update 2017-12-21 External Id SAMEA104446671 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2017-12-21T17:03:29Z INSDC last update 2017-12-21T11:25:49Z INSDC status public Submitter Id E-MTAB-6214:Sample 6 broker name ArrayExpress cell type mononuclear cell common name human disease metastatic melanoma organism part blood phenotype CD14+CD16-HLA-DRhiLin- response to treatment responder sample name E-MTAB-6214:Sample 6 treatment anti-PD-1 immunotherapy ERS2064614 SAMEA104446672 9606 Homo sapiens Protocols: CD14+CD16-HLA-DRhiLin- (CD3, CD4, CD19, CD45RO) monocytes were sorted from frozen PBMC form blood samples from HD (healthy donor), R (responder) and NR (non-responder) at baseline. Representative samples (n=4, each) of responders/non responders/ and healthy donors were selected from archival samples stored in the dermatology biobank according to the same clinical criteria used in the discovery and validation cohorts for CyTOF and FACS analysis. RNA was isolated using the RNA plus micro kit (Qiagen). We used the SMARTer Stranded total rna-seq pico kit (Clontech) for RNA preparations. Libraries for sequencing were generated with the TruSeq RNA Sample Preparation kit (Illumina). ENA first public 2017-12-21 ENA last update 2017-12-21 External Id SAMEA104446672 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2017-12-21T17:03:29Z INSDC last update 2017-12-21T11:25:49Z INSDC status public Submitter Id E-MTAB-6214:Sample 7 broker name ArrayExpress cell type mononuclear cell common name human disease metastatic melanoma organism part blood phenotype CD14+CD16-HLA-DRhiLin- response to treatment responder sample name E-MTAB-6214:Sample 7 treatment anti-PD-1 immunotherapy ERS2064615 SAMEA104446673 9606 Homo sapiens Protocols: CD14+CD16-HLA-DRhiLin- (CD3, CD4, CD19, CD45RO) monocytes were sorted from frozen PBMC form blood samples from HD (healthy donor), R (responder) and NR (non-responder) at baseline. Representative samples (n=4, each) of responders/non responders/ and healthy donors were selected from archival samples stored in the dermatology biobank according to the same clinical criteria used in the discovery and validation cohorts for CyTOF and FACS analysis. RNA was isolated using the RNA plus micro kit (Qiagen). We used the SMARTer Stranded total rna-seq pico kit (Clontech) for RNA preparations. Libraries for sequencing were generated with the TruSeq RNA Sample Preparation kit (Illumina). ENA first public 2017-12-21 ENA last update 2017-12-21 External Id SAMEA104446673 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2017-12-21T17:03:29Z INSDC last update 2017-12-21T11:25:49Z INSDC status public Submitter Id E-MTAB-6214:Sample 8 broker name ArrayExpress cell type mononuclear cell common name human disease metastatic melanoma organism part blood phenotype CD14+CD16-HLA-DRhiLin- response to treatment responder sample name E-MTAB-6214:Sample 8 treatment anti-PD-1 immunotherapy ERS2064616 SAMEA104446674 9606 Homo sapiens Protocols: CD14+CD16-HLA-DRhiLin- (CD3, CD4, CD19, CD45RO) monocytes were sorted from frozen PBMC form blood samples from HD (healthy donor), R (responder) and NR (non-responder) at baseline. Representative samples (n=4, each) of responders/non responders/ and healthy donors were selected from archival samples stored in the dermatology biobank according to the same clinical criteria used in the discovery and validation cohorts for CyTOF and FACS analysis. RNA was isolated using the RNA plus micro kit (Qiagen). We used the SMARTer Stranded total rna-seq pico kit (Clontech) for RNA preparations. Libraries for sequencing were generated with the TruSeq RNA Sample Preparation kit (Illumina). ENA first public 2017-12-21 ENA last update 2017-12-21 External Id SAMEA104446674 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2017-12-21T17:03:29Z INSDC last update 2017-12-21T11:25:49Z INSDC status public Submitter Id E-MTAB-6214:Sample 9 broker name ArrayExpress cell type mononuclear cell common name human disease metastatic melanoma organism part blood phenotype CD14+CD16-HLA-DRhiLin- response to treatment non-responder sample name E-MTAB-6214:Sample 9 treatment anti-PD-1 immunotherapy ERS2064617 SAMEA104446675 9606 Homo sapiens Protocols: CD14+CD16-HLA-DRhiLin- (CD3, CD4, CD19, CD45RO) monocytes were sorted from frozen PBMC form blood samples from HD (healthy donor), R (responder) and NR (non-responder) at baseline. Representative samples (n=4, each) of responders/non responders/ and healthy donors were selected from archival samples stored in the dermatology biobank according to the same clinical criteria used in the discovery and validation cohorts for CyTOF and FACS analysis. RNA was isolated using the RNA plus micro kit (Qiagen). We used the SMARTer Stranded total rna-seq pico kit (Clontech) for RNA preparations. Libraries for sequencing were generated with the TruSeq RNA Sample Preparation kit (Illumina). ENA first public 2017-12-21 ENA last update 2017-12-21 External Id SAMEA104446675 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2017-12-21T17:03:29Z INSDC last update 2017-12-21T11:25:50Z INSDC status public Submitter Id E-MTAB-6214:Sample 1 broker name ArrayExpress cell type mononuclear cell common name human disease normal organism part blood phenotype CD14+CD16-HLA-DRhiLin- sample name E-MTAB-6214:Sample 1 ERS2064618 SAMEA104446676 9606 Homo sapiens Protocols: CD14+CD16-HLA-DRhiLin- (CD3, CD4, CD19, CD45RO) monocytes were sorted from frozen PBMC form blood samples from HD (healthy donor), R (responder) and NR (non-responder) at baseline. Representative samples (n=4, each) of responders/non responders/ and healthy donors were selected from archival samples stored in the dermatology biobank according to the same clinical criteria used in the discovery and validation cohorts for CyTOF and FACS analysis. RNA was isolated using the RNA plus micro kit (Qiagen). We used the SMARTer Stranded total rna-seq pico kit (Clontech) for RNA preparations. Libraries for sequencing were generated with the TruSeq RNA Sample Preparation kit (Illumina). ENA first public 2017-12-21 ENA last update 2017-12-21 External Id SAMEA104446676 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2017-12-21T17:03:29Z INSDC last update 2017-12-21T11:25:50Z INSDC status public Submitter Id E-MTAB-6214:Sample 2 broker name ArrayExpress cell type mononuclear cell common name human disease normal organism part blood phenotype CD14+CD16-HLA-DRhiLin- sample name E-MTAB-6214:Sample 2 ERS2064619 SAMEA104446677 9606 Homo sapiens Protocols: CD14+CD16-HLA-DRhiLin- (CD3, CD4, CD19, CD45RO) monocytes were sorted from frozen PBMC form blood samples from HD (healthy donor), R (responder) and NR (non-responder) at baseline. Representative samples (n=4, each) of responders/non responders/ and healthy donors were selected from archival samples stored in the dermatology biobank according to the same clinical criteria used in the discovery and validation cohorts for CyTOF and FACS analysis. RNA was isolated using the RNA plus micro kit (Qiagen). We used the SMARTer Stranded total rna-seq pico kit (Clontech) for RNA preparations. Libraries for sequencing were generated with the TruSeq RNA Sample Preparation kit (Illumina). ENA first public 2017-12-21 ENA last update 2017-12-21 External Id SAMEA104446677 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2017-12-21T17:03:29Z INSDC last update 2017-12-21T11:25:50Z INSDC status public Submitter Id E-MTAB-6214:Sample 3 broker name ArrayExpress cell type mononuclear cell common name human disease normal organism part blood phenotype CD14+CD16-HLA-DRhiLin- sample name E-MTAB-6214:Sample 3 ERS2064620 SAMEA104446678 9606 Homo sapiens Protocols: CD14+CD16-HLA-DRhiLin- (CD3, CD4, CD19, CD45RO) monocytes were sorted from frozen PBMC form blood samples from HD (healthy donor), R (responder) and NR (non-responder) at baseline. Representative samples (n=4, each) of responders/non responders/ and healthy donors were selected from archival samples stored in the dermatology biobank according to the same clinical criteria used in the discovery and validation cohorts for CyTOF and FACS analysis. RNA was isolated using the RNA plus micro kit (Qiagen). We used the SMARTer Stranded total rna-seq pico kit (Clontech) for RNA preparations. Libraries for sequencing were generated with the TruSeq RNA Sample Preparation kit (Illumina). ENA first public 2017-12-21 ENA last update 2017-12-21 External Id SAMEA104446678 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2017-12-21T17:03:29Z INSDC last update 2017-12-21T11:25:50Z INSDC status public Submitter Id E-MTAB-6214:Sample 4 broker name ArrayExpress cell type mononuclear cell common name human disease normal organism part blood phenotype CD14+CD16-HLA-DRhiLin- sample name E-MTAB-6214:Sample 4