ERS2065951 SAMEA104448009 100272 uncultured eukaryote Sample of undisturbed boreal forest soil (ecotype D1), refrigerated until sequencing ENA first public 2018-12-19 ENA last update 2017-12-21 External Id SAMEA104448009 INSDC center alias IBENS INSDC center name Institut de Biologie de Ecole normale superieure (IBENS), France INSDC first public 2018-12-19T17:03:06Z INSDC last update 2017-12-21T18:12:03Z INSDC status public Submitter Id D1-org_S6_L001 collection date 2013-07-15 environment (biome) taiga (boreal forest) environment (feature) above Albertan bitumen ore deposit environment (material) forest soil environmental sample Yes further details http://unieuk.org/project/ geographic location (country and/or sea) Canada geographic location (latitude) 57.147611 geographic location (longitude) -111.544000 geographic location (region and locality) Northeastern Alberta pcr primers TAReuk454FWD1 (5′-CCAGCA(G/C)C(C/T)GCGGTAATTCC-3'), TAReukREV3 (5′-ACTTTCGTTCTTGAT(C/T)(A/G)A-3′) ph (topsoil) 5.4 sample collection device or method Each site was sampled on 3 points located at 10 meters from each other to have 3 biological replicates. Each replicate was composed of four locations taken on the edges of a 10 cm square. At each location, the organic layer and the top 5 cm of mineral soil were sampled separately. About 100 mL of soil was collected for each replicate in a sterile Ziploc plastic bag, attempting not to disturb the internal sEach site was sampled on 3 points located at 10 meters from each other to have 3 biological replicates. Each replicate was composed of four locations taken on the edges of a 10 cm square. At each location, the organic layer and the top 5 cm of mineral soil were sampled separately. About 100 mL of soil was collected for each replicate in a sterile Ziploc plastic bag, attempting not to disturb the internal structure of the sample.tructure of the sample. sample name D1-org_S6_L001 site D1 soil layer Organic storage refrigerated target gene 18S target subfragment V4 treated no ERS2065952 SAMEA104448010 100272 uncultured eukaryote Sample of undisturbed boreal forest soil (ecotype D1), refrigerated until sequencing ENA first public 2018-12-19 ENA last update 2017-12-21 External Id SAMEA104448010 INSDC center alias IBENS INSDC center name Institut de Biologie de Ecole normale superieure (IBENS), France INSDC first public 2018-12-19T17:03:06Z INSDC last update 2017-12-21T18:12:19Z INSDC status public Submitter Id D1-O2_S18_L001 collection date 2013-07-15 environment (biome) taiga (boreal forest) environment (feature) above Albertan bitumen ore deposit environment (material) forest soil environmental sample Yes further details http://unieuk.org/project/ geographic location (country and/or sea) Canada geographic location (latitude) 57.147611 geographic location (longitude) -111.544000 geographic location (region and locality) Northeastern Alberta pcr primers TAReuk454FWD1 (5′-CCAGCA(G/C)C(C/T)GCGGTAATTCC-3'), TAReukREV3 (5′-ACTTTCGTTCTTGAT(C/T)(A/G)A-3′) ph (topsoil) 5.4 sample collection device or method Each site was sampled on 3 points located at 10 meters from each other to have 3 biological replicates. Each replicate was composed of four locations taken on the edges of a 10 cm square. At each location, the organic layer and the top 5 cm of mineral soil were sampled separately. About 100 mL of soil was collected for each replicate in a sterile Ziploc plastic bag, attempting not to disturb the internal sEach site was sampled on 3 points located at 10 meters from each other to have 3 biological replicates. Each replicate was composed of four locations taken on the edges of a 10 cm square. At each location, the organic layer and the top 5 cm of mineral soil were sampled separately. About 100 mL of soil was collected for each replicate in a sterile Ziploc plastic bag, attempting not to disturb the internal structure of the sample.tructure of the sample. sample name D1-O2_S18_L001 site D1 soil layer Organic storage refrigerated target gene 18S target subfragment V4 treated no ERS2065953 SAMEA104448011 100272 uncultured eukaryote Sample of undisturbed boreal forest soil (ecotype D1), refrigerated until sequencing ENA first public 2018-12-19 ENA last update 2017-12-21 External Id SAMEA104448011 INSDC center alias IBENS INSDC center name Institut de Biologie de Ecole normale superieure (IBENS), France INSDC first public 2018-12-19T17:03:06Z INSDC last update 2017-12-21T18:12:36Z INSDC status public Submitter Id D1-O3_S19_L001 collection date 2013-07-15 environment (biome) taiga (boreal forest) environment (feature) above Albertan bitumen ore deposit environment (material) forest soil environmental sample Yes further details http://unieuk.org/project/ geographic location (country and/or sea) Canada geographic location (latitude) 57.147611 geographic location (longitude) -111.544000 geographic location (region and locality) Northeastern Alberta pcr primers TAReuk454FWD1 (5′-CCAGCA(G/C)C(C/T)GCGGTAATTCC-3'), TAReukREV3 (5′-ACTTTCGTTCTTGAT(C/T)(A/G)A-3′) ph (topsoil) 5.4 sample collection device or method Each site was sampled on 3 points located at 10 meters from each other to have 3 biological replicates. Each replicate was composed of four locations taken on the edges of a 10 cm square. At each location, the organic layer and the top 5 cm of mineral soil were sampled separately. About 100 mL of soil was collected for each replicate in a sterile Ziploc plastic bag, attempting not to disturb the internal sEach site was sampled on 3 points located at 10 meters from each other to have 3 biological replicates. Each replicate was composed of four locations taken on the edges of a 10 cm square. At each location, the organic layer and the top 5 cm of mineral soil were sampled separately. About 100 mL of soil was collected for each replicate in a sterile Ziploc plastic bag, attempting not to disturb the internal structure of the sample.tructure of the sample. sample name D1-O3_S19_L001 site D1 soil layer Organic storage refrigerated target gene 18S target subfragment V4 treated no ERS2065954 SAMEA104448012 100272 uncultured eukaryote Sample of undisturbed boreal forest soil (ecotype D1), refrigerated until sequencing ENA first public 2018-12-19 ENA last update 2017-12-21 External Id SAMEA104448012 INSDC center alias IBENS INSDC center name Institut de Biologie de Ecole normale superieure (IBENS), France INSDC first public 2018-12-19T17:03:06Z INSDC last update 2017-12-21T17:41:55Z INSDC status public Submitter Id D1-min_S5_L001 collection date 2013-07-15 environment (biome) taiga (boreal forest) environment (feature) above Albertan bitumen ore deposit environment (material) forest soil environmental sample Yes further details http://unieuk.org/project/ geographic location (country and/or sea) Canada geographic location (latitude) 57.147611 geographic location (longitude) -111.544000 geographic location (region and locality) Northeastern Alberta pcr primers TAReuk454FWD1 (5′-CCAGCA(G/C)C(C/T)GCGGTAATTCC-3'), TAReukREV3 (5′-ACTTTCGTTCTTGAT(C/T)(A/G)A-3′) ph (topsoil) 5.4 sample collection device or method Each site was sampled on 3 points located at 10 meters from each other to have 3 biological replicates. Each replicate was composed of four locations taken on the edges of a 10 cm square. At each location, the organic layer and the top 5 cm of mineral soil were sampled separately. About 100 mL of soil was collected for each replicate in a sterile Ziploc plastic bag, attempting not to disturb the internal sEach site was sampled on 3 points located at 10 meters from each other to have 3 biological replicates. Each replicate was composed of four locations taken on the edges of a 10 cm square. At each location, the organic layer and the top 5 cm of mineral soil were sampled separately. About 100 mL of soil was collected for each replicate in a sterile Ziploc plastic bag, attempting not to disturb the internal structure of the sample.tructure of the sample. sample name D1-min_S5_L001 site D1 soil layer Mineral storage refrigerated target gene 18S target subfragment V4 treated no ERS2065955 SAMEA104448013 100272 uncultured eukaryote Sample of undisturbed boreal forest soil (ecotype D1), refrigerated until sequencing ENA first public 2018-12-19 ENA last update 2017-12-21 External Id SAMEA104448013 INSDC center alias IBENS INSDC center name Institut de Biologie de Ecole normale superieure (IBENS), France INSDC first public 2018-12-19T17:03:06Z INSDC last update 2017-12-21T18:11:34Z INSDC status public Submitter Id D1-M2_S20_L001 collection date 2013-07-15 environment (biome) taiga (boreal forest) environment (feature) above Albertan bitumen ore deposit environment (material) forest soil environmental sample Yes further details http://unieuk.org/project/ geographic location (country and/or sea) Canada geographic location (latitude) 57.147611 geographic location (longitude) -111.544000 geographic location (region and locality) Northeastern Alberta pcr primers TAReuk454FWD1 (5′-CCAGCA(G/C)C(C/T)GCGGTAATTCC-3'), TAReukREV3 (5′-ACTTTCGTTCTTGAT(C/T)(A/G)A-3′) ph (topsoil) 5.4 sample collection device or method Each site was sampled on 3 points located at 10 meters from each other to have 3 biological replicates. Each replicate was composed of four locations taken on the edges of a 10 cm square. At each location, the organic layer and the top 5 cm of mineral soil were sampled separately. About 100 mL of soil was collected for each replicate in a sterile Ziploc plastic bag, attempting not to disturb the internal sEach site was sampled on 3 points located at 10 meters from each other to have 3 biological replicates. Each replicate was composed of four locations taken on the edges of a 10 cm square. At each location, the organic layer and the top 5 cm of mineral soil were sampled separately. About 100 mL of soil was collected for each replicate in a sterile Ziploc plastic bag, attempting not to disturb the internal structure of the sample.tructure of the sample. sample name D1-M2_S20_L001 site D1 soil layer Mineral storage refrigerated target gene 18S target subfragment V4 treated no ERS2065956 SAMEA104448014 100272 uncultured eukaryote Sample of undisturbed boreal forest soil (ecotype D1), refrigerated until sequencing ENA first public 2018-12-19 ENA last update 2017-12-21 External Id SAMEA104448014 INSDC center alias IBENS INSDC center name Institut de Biologie de Ecole normale superieure (IBENS), France INSDC first public 2018-12-19T17:03:06Z INSDC last update 2017-12-21T18:11:14Z INSDC status public Submitter Id D1-M3_S21_L001 collection date 2013-07-15 environment (biome) taiga (boreal forest) environment (feature) above Albertan bitumen ore deposit environment (material) forest soil environmental sample Yes further details http://unieuk.org/project/ geographic location (country and/or sea) Canada geographic location (latitude) 57.147611 geographic location (longitude) -111.544000 geographic location (region and locality) Northeastern Alberta pcr primers TAReuk454FWD1 (5′-CCAGCA(G/C)C(C/T)GCGGTAATTCC-3'), TAReukREV3 (5′-ACTTTCGTTCTTGAT(C/T)(A/G)A-3′) ph (topsoil) 5.4 sample collection device or method Each site was sampled on 3 points located at 10 meters from each other to have 3 biological replicates. Each replicate was composed of four locations taken on the edges of a 10 cm square. At each location, the organic layer and the top 5 cm of mineral soil were sampled separately. About 100 mL of soil was collected for each replicate in a sterile Ziploc plastic bag, attempting not to disturb the internal sEach site was sampled on 3 points located at 10 meters from each other to have 3 biological replicates. Each replicate was composed of four locations taken on the edges of a 10 cm square. At each location, the organic layer and the top 5 cm of mineral soil were sampled separately. About 100 mL of soil was collected for each replicate in a sterile Ziploc plastic bag, attempting not to disturb the internal structure of the sample.tructure of the sample. sample name D1-M3_S21_L001 site D1 soil layer Mineral storage refrigerated target gene 18S target subfragment V4 treated no ERS2065957 SAMEA104448015 100272 uncultured eukaryote Sample of undisturbed boreal forest soil (ecotype D1), kept at -80°C until sequencing ENA first public 2018-12-19 ENA last update 2017-12-21 External Id SAMEA104448015 INSDC center alias IBENS INSDC center name Institut de Biologie de Ecole normale superieure (IBENS), France INSDC first public 2018-12-19T17:03:06Z INSDC last update 2017-12-21T18:10:55Z INSDC status public Submitter Id MAT-org_S8_L001 averaged total c 39.58 averaged total n 2.47 collection date 2013-07-15 environment (biome) taiga (boreal forest) environment (feature) above Albertan bitumen ore deposit environment (material) forest soil environmental sample Yes further details http://unieuk.org/project/ geographic location (country and/or sea) Canada geographic location (latitude) 57.147611 geographic location (longitude) -111.544000 geographic location (region and locality) Northeastern Alberta pcr primers TAReuk454FWD1 (5′-CCAGCA(G/C)C(C/T)GCGGTAATTCC-3'), TAReukREV3 (5′-ACTTTCGTTCTTGAT(C/T)(A/G)A-3′) ph (topsoil) 5.4 sample collection device or method Each site was sampled on 3 points located at 10 meters from each other to have 3 biological replicates. Each replicate was composed of four locations taken on the edges of a 10 cm square. At each location, the organic layer and the top 5 cm of mineral soil were sampled separately. About 100 mL of soil was collected for each replicate in a sterile Ziploc plastic bag, attempting not to disturb the internal sEach site was sampled on 3 points located at 10 meters from each other to have 3 biological replicates. Each replicate was composed of four locations taken on the edges of a 10 cm square. At each location, the organic layer and the top 5 cm of mineral soil were sampled separately. About 100 mL of soil was collected for each replicate in a sterile Ziploc plastic bag, attempting not to disturb the internal structure of the sample.tructure of the sample. sample name MAT-org_S8_L001 site D1 soil layer Organic storage frozen target gene 18S target subfragment V4 treated no ERS2065958 SAMEA104448016 100272 uncultured eukaryote Sample of undisturbed boreal forest soil (ecotype D1), kept at -80°C until sequencing ENA first public 2018-12-19 ENA last update 2017-12-21 External Id SAMEA104448016 INSDC center alias IBENS INSDC center name Institut de Biologie de Ecole normale superieure (IBENS), France INSDC first public 2018-12-19T17:03:06Z INSDC last update 2017-12-21T18:10:35Z INSDC status public Submitter Id MAT-1-O2_S9_L001 averaged total c 20.24 averaged total n 1.05 collection date 2013-07-15 environment (biome) taiga (boreal forest) environment (feature) above Albertan bitumen ore deposit environment (material) forest soil environmental sample Yes further details http://unieuk.org/project/ geographic location (country and/or sea) Canada geographic location (latitude) 57.147611 geographic location (longitude) -111.544000 geographic location (region and locality) Northeastern Alberta pcr primers TAReuk454FWD1 (5′-CCAGCA(G/C)C(C/T)GCGGTAATTCC-3'), TAReukREV3 (5′-ACTTTCGTTCTTGAT(C/T)(A/G)A-3′) ph (topsoil) 5.4 sample collection device or method Each site was sampled on 3 points located at 10 meters from each other to have 3 biological replicates. Each replicate was composed of four locations taken on the edges of a 10 cm square. At each location, the organic layer and the top 5 cm of mineral soil were sampled separately. About 100 mL of soil was collected for each replicate in a sterile Ziploc plastic bag, attempting not to disturb the internal sEach site was sampled on 3 points located at 10 meters from each other to have 3 biological replicates. Each replicate was composed of four locations taken on the edges of a 10 cm square. At each location, the organic layer and the top 5 cm of mineral soil were sampled separately. About 100 mL of soil was collected for each replicate in a sterile Ziploc plastic bag, attempting not to disturb the internal structure of the sample.tructure of the sample. sample name MAT-1-O2_S9_L001 site D1 soil layer Organic storage frozen target gene 18S target subfragment V4 treated no ERS2065959 SAMEA104448017 100272 uncultured eukaryote Sample of undisturbed boreal forest soil (ecotype D1), kept at -80°C until sequencing ENA first public 2018-12-19 ENA last update 2017-12-21 External Id SAMEA104448017 INSDC center alias IBENS INSDC center name Institut de Biologie de Ecole normale superieure (IBENS), France INSDC first public 2018-12-19T17:03:06Z INSDC last update 2017-12-21T18:10:13Z INSDC status public Submitter Id MAT-1-O3_S10_L001 averaged total c 19.58 averaged total n 0.92 collection date 2013-07-15 environment (biome) taiga (boreal forest) environment (feature) above Albertan bitumen ore deposit environment (material) forest soil environmental sample Yes further details http://unieuk.org/project/ geographic location (country and/or sea) Canada geographic location (latitude) 57.147611 geographic location (longitude) -111.544000 geographic location (region and locality) Northeastern Alberta pcr primers TAReuk454FWD1 (5′-CCAGCA(G/C)C(C/T)GCGGTAATTCC-3'), TAReukREV3 (5′-ACTTTCGTTCTTGAT(C/T)(A/G)A-3′) ph (topsoil) 5.4 sample collection device or method Each site was sampled on 3 points located at 10 meters from each other to have 3 biological replicates. Each replicate was composed of four locations taken on the edges of a 10 cm square. At each location, the organic layer and the top 5 cm of mineral soil were sampled separately. About 100 mL of soil was collected for each replicate in a sterile Ziploc plastic bag, attempting not to disturb the internal sEach site was sampled on 3 points located at 10 meters from each other to have 3 biological replicates. Each replicate was composed of four locations taken on the edges of a 10 cm square. At each location, the organic layer and the top 5 cm of mineral soil were sampled separately. About 100 mL of soil was collected for each replicate in a sterile Ziploc plastic bag, attempting not to disturb the internal structure of the sample.tructure of the sample. sample name MAT-1-O3_S10_L001 site D1 soil layer Organic storage frozen target gene 18S target subfragment V4 treated no ERS2065960 SAMEA104448018 100272 uncultured eukaryote Sample of undisturbed boreal forest soil (ecotype D1), kept at -80°C until sequencing ENA first public 2018-12-19 ENA last update 2017-12-21 External Id SAMEA104448018 INSDC center alias IBENS INSDC center name Institut de Biologie de Ecole normale superieure (IBENS), France INSDC first public 2018-12-19T17:03:06Z INSDC last update 2017-12-21T18:09:51Z INSDC status public Submitter Id MAT-min_S7_L001 averaged total c 1.39 averaged total n 0.06 collection date 2013-07-15 environment (biome) taiga (boreal forest) environment (feature) above Albertan bitumen ore deposit environment (material) forest soil environmental sample Yes further details http://unieuk.org/project/ geographic location (country and/or sea) Canada geographic location (latitude) 57.147611 geographic location (longitude) -111.544000 geographic location (region and locality) Northeastern Alberta pcr primers TAReuk454FWD1 (5′-CCAGCA(G/C)C(C/T)GCGGTAATTCC-3'), TAReukREV3 (5′-ACTTTCGTTCTTGAT(C/T)(A/G)A-3′) ph (topsoil) 5.4 sample collection device or method Each site was sampled on 3 points located at 10 meters from each other to have 3 biological replicates. Each replicate was composed of four locations taken on the edges of a 10 cm square. At each location, the organic layer and the top 5 cm of mineral soil were sampled separately. About 100 mL of soil was collected for each replicate in a sterile Ziploc plastic bag, attempting not to disturb the internal sEach site was sampled on 3 points located at 10 meters from each other to have 3 biological replicates. Each replicate was composed of four locations taken on the edges of a 10 cm square. At each location, the organic layer and the top 5 cm of mineral soil were sampled separately. About 100 mL of soil was collected for each replicate in a sterile Ziploc plastic bag, attempting not to disturb the internal structure of the sample.tructure of the sample. sample name MAT-min_S7_L001 site D1 soil layer Mineral storage frozen target gene 18S target subfragment V4 treated no ERS2065961 SAMEA104448019 100272 uncultured eukaryote Sample of undisturbed boreal forest soil (ecotype D1), kept at -80°C until sequencing ENA first public 2018-12-19 ENA last update 2017-12-21 External Id SAMEA104448019 INSDC center alias IBENS INSDC center name Institut de Biologie de Ecole normale superieure (IBENS), France INSDC first public 2018-12-19T17:03:06Z INSDC last update 2017-12-21T18:08:38Z INSDC status public Submitter Id MAT-1-M2_S11_L001 averaged total c 0.56 averaged total n 0.04 collection date 2013-07-15 environment (biome) taiga (boreal forest) environment (feature) above Albertan bitumen ore deposit environment (material) forest soil environmental sample Yes further details http://unieuk.org/project/ geographic location (country and/or sea) Canada geographic location (latitude) 57.147611 geographic location (longitude) -111.544000 geographic location (region and locality) Northeastern Alberta pcr primers TAReuk454FWD1 (5′-CCAGCA(G/C)C(C/T)GCGGTAATTCC-3'), TAReukREV3 (5′-ACTTTCGTTCTTGAT(C/T)(A/G)A-3′) ph (topsoil) 5.4 sample collection device or method Each site was sampled on 3 points located at 10 meters from each other to have 3 biological replicates. Each replicate was composed of four locations taken on the edges of a 10 cm square. At each location, the organic layer and the top 5 cm of mineral soil were sampled separately. About 100 mL of soil was collected for each replicate in a sterile Ziploc plastic bag, attempting not to disturb the internal sEach site was sampled on 3 points located at 10 meters from each other to have 3 biological replicates. Each replicate was composed of four locations taken on the edges of a 10 cm square. At each location, the organic layer and the top 5 cm of mineral soil were sampled separately. About 100 mL of soil was collected for each replicate in a sterile Ziploc plastic bag, attempting not to disturb the internal structure of the sample.tructure of the sample. sample name MAT-1-M2_S11_L001 site D1 soil layer Mineral storage frozen target gene 18S target subfragment V4 treated no ERS2065962 SAMEA104448020 100272 uncultured eukaryote Sample of undisturbed boreal forest soil (ecotype D1), kept at -80°C until sequencing ENA first public 2018-12-19 ENA last update 2017-12-21 External Id SAMEA104448020 INSDC center alias IBENS INSDC center name Institut de Biologie de Ecole normale superieure (IBENS), France INSDC first public 2018-12-19T17:03:06Z INSDC last update 2017-12-21T18:08:09Z INSDC status public Submitter Id MAT-1-M3_S12_L001 averaged total c 3.46 averaged total n 0.08 collection date 2013-07-15 environment (biome) taiga (boreal forest) environment (feature) above Albertan bitumen ore deposit environment (material) forest soil environmental sample Yes further details http://unieuk.org/project/ geographic location (country and/or sea) Canada geographic location (latitude) 57.147611 geographic location (longitude) -111.544000 geographic location (region and locality) Northeastern Alberta pcr primers TAReuk454FWD1 (5′-CCAGCA(G/C)C(C/T)GCGGTAATTCC-3'), TAReukREV3 (5′-ACTTTCGTTCTTGAT(C/T)(A/G)A-3′) ph (topsoil) 5.4 sample collection device or method Each site was sampled on 3 points located at 10 meters from each other to have 3 biological replicates. Each replicate was composed of four locations taken on the edges of a 10 cm square. At each location, the organic layer and the top 5 cm of mineral soil were sampled separately. About 100 mL of soil was collected for each replicate in a sterile Ziploc plastic bag, attempting not to disturb the internal sEach site was sampled on 3 points located at 10 meters from each other to have 3 biological replicates. Each replicate was composed of four locations taken on the edges of a 10 cm square. At each location, the organic layer and the top 5 cm of mineral soil were sampled separately. About 100 mL of soil was collected for each replicate in a sterile Ziploc plastic bag, attempting not to disturb the internal structure of the sample.tructure of the sample. sample name MAT-1-M3_S12_L001 site D1 soil layer Mineral storage frozen target gene 18S target subfragment V4 treated no ERS2065963 SAMEA104448021 100272 uncultured eukaryote Sample of reclaimed boreal forest soil (ecotype Rec1 : peat over salvaged mineral soil lying above tailings (after >1m); placed 6 years before sampling), kept at -80°C until sequencing ENA first public 2018-12-19 ENA last update 2017-12-21 External Id SAMEA104448021 INSDC center alias IBENS INSDC center name Institut de Biologie de Ecole normale superieure (IBENS), France INSDC first public 2018-12-19T17:03:06Z INSDC last update 2017-12-21T17:50:10Z INSDC status public Submitter Id Rec1-org_S12_L001 averaged total c 8.75 averaged total n 0.5 collection date 2013-07-18 environment (biome) taiga (boreal forest) environment (feature) above Albertan bitumen ore deposit environment (material) forest soil environmental sample Yes further details http://unieuk.org/project/ geographic location (country and/or sea) Canada geographic location (latitude) 57.019578 geographic location (longitude) -111.728472 geographic location (region and locality) Northeastern Alberta pcr primers TAReuk454FWD1 (5′-CCAGCA(G/C)C(C/T)GCGGTAATTCC-3'), TAReukREV3 (5′-ACTTTCGTTCTTGAT(C/T)(A/G)A-3′) ph (topsoil) 5.9 sample collection device or method Each site was sampled on 3 points located at 10 meters from each other to have 3 biological replicates. Each replicate was composed of four locations taken on the edges of a 10 cm square. At each location, the organic layer and the top 5 cm of mineral soil were sampled separately. About 100 mL of soil was collected for each replicate in a sterile Ziploc plastic bag, attempting not to disturb the internal sEach site was sampled on 3 points located at 10 meters from each other to have 3 biological replicates. Each replicate was composed of four locations taken on the edges of a 10 cm square. At each location, the organic layer and the top 5 cm of mineral soil were sampled separately. About 100 mL of soil was collected for each replicate in a sterile Ziploc plastic bag, attempting not to disturb the internal structure of the sample.tructure of the sample. sample name Rec1-org_S12_L001 site Rec1 soil layer Organic storage frozen target gene 18S target subfragment V4 treated yes treatment reclamation 1, 6 years before sampling ERS2065964 SAMEA104448022 100272 uncultured eukaryote Sample of reclaimed boreal forest soil (ecotype Rec1 : peat over salvaged mineral soil lying above tailings (after >1m); placed 6 years before sampling), kept at -80°C until sequencing ENA first public 2018-12-19 ENA last update 2017-12-21 External Id SAMEA104448022 INSDC center alias IBENS INSDC center name Institut de Biologie de Ecole normale superieure (IBENS), France INSDC first public 2018-12-19T17:03:06Z INSDC last update 2017-12-21T17:49:45Z INSDC status public Submitter Id Rec1-O2_S5_L001 averaged total c 23.87 averaged total n 1.18 collection date 2013-07-18 environment (biome) taiga (boreal forest) environment (feature) above Albertan bitumen ore deposit environment (material) forest soil environmental sample Yes further details http://unieuk.org/project/ geographic location (country and/or sea) Canada geographic location (latitude) 57.019578 geographic location (longitude) -111.728472 geographic location (region and locality) Northeastern Alberta pcr primers TAReuk454FWD1 (5′-CCAGCA(G/C)C(C/T)GCGGTAATTCC-3'), TAReukREV3 (5′-ACTTTCGTTCTTGAT(C/T)(A/G)A-3′) ph (topsoil) 5.9 sample collection device or method Each site was sampled on 3 points located at 10 meters from each other to have 3 biological replicates. Each replicate was composed of four locations taken on the edges of a 10 cm square. At each location, the organic layer and the top 5 cm of mineral soil were sampled separately. About 100 mL of soil was collected for each replicate in a sterile Ziploc plastic bag, attempting not to disturb the internal sEach site was sampled on 3 points located at 10 meters from each other to have 3 biological replicates. Each replicate was composed of four locations taken on the edges of a 10 cm square. At each location, the organic layer and the top 5 cm of mineral soil were sampled separately. About 100 mL of soil was collected for each replicate in a sterile Ziploc plastic bag, attempting not to disturb the internal structure of the sample.tructure of the sample. sample name Rec1-O2_S5_L001 site Rec1 soil layer Organic storage frozen target gene 18S target subfragment V4 treated yes treatment reclamation 1, 6 years before sampling ERS2065965 SAMEA104448023 100272 uncultured eukaryote Sample of reclaimed boreal forest soil (ecotype Rec1 : peat over salvaged mineral soil lying above tailings (after >1m); placed 6 years before sampling), kept at -80°C until sequencing ENA first public 2018-12-19 ENA last update 2017-12-21 External Id SAMEA104448023 INSDC center alias IBENS INSDC center name Institut de Biologie de Ecole normale superieure (IBENS), France INSDC first public 2018-12-19T17:03:06Z INSDC last update 2017-12-21T17:49:24Z INSDC status public Submitter Id Rec1-O3_S6_L001 averaged total c 23.98 averaged total n 1.21 collection date 2013-07-18 environment (biome) taiga (boreal forest) environment (feature) above Albertan bitumen ore deposit environment (material) forest soil environmental sample Yes further details http://unieuk.org/project/ geographic location (country and/or sea) Canada geographic location (latitude) 57.019578 geographic location (longitude) -111.728472 geographic location (region and locality) Northeastern Alberta pcr primers TAReuk454FWD1 (5′-CCAGCA(G/C)C(C/T)GCGGTAATTCC-3'), TAReukREV3 (5′-ACTTTCGTTCTTGAT(C/T)(A/G)A-3′) ph (topsoil) 5.9 sample collection device or method Each site was sampled on 3 points located at 10 meters from each other to have 3 biological replicates. Each replicate was composed of four locations taken on the edges of a 10 cm square. At each location, the organic layer and the top 5 cm of mineral soil were sampled separately. About 100 mL of soil was collected for each replicate in a sterile Ziploc plastic bag, attempting not to disturb the internal sEach site was sampled on 3 points located at 10 meters from each other to have 3 biological replicates. Each replicate was composed of four locations taken on the edges of a 10 cm square. At each location, the organic layer and the top 5 cm of mineral soil were sampled separately. About 100 mL of soil was collected for each replicate in a sterile Ziploc plastic bag, attempting not to disturb the internal structure of the sample.tructure of the sample. sample name Rec1-O3_S6_L001 site Rec1 soil layer Organic storage frozen target gene 18S target subfragment V4 treated yes treatment reclamation 1, 6 years before sampling ERS2065966 SAMEA104448024 100272 uncultured eukaryote Sample of reclaimed boreal forest soil (ecotype Rec1 : peat over salvaged mineral soil lying above tailings (after >1m); placed 6 years before sampling), kept at -80°C until sequencing ENA first public 2018-12-19 ENA last update 2017-12-21 External Id SAMEA104448024 INSDC center alias IBENS INSDC center name Institut de Biologie de Ecole normale superieure (IBENS), France INSDC first public 2018-12-19T17:03:06Z INSDC last update 2017-12-21T17:48:51Z INSDC status public Submitter Id Rec1-min_S11_L001 averaged total c 2.24 averaged total n 0.11 collection date 2013-07-18 environment (biome) taiga (boreal forest) environment (feature) above Albertan bitumen ore deposit environment (material) forest soil environmental sample Yes further details http://unieuk.org/project/ geographic location (country and/or sea) Canada geographic location (latitude) 57.019578 geographic location (longitude) -111.728472 geographic location (region and locality) Northeastern Alberta pcr primers TAReuk454FWD1 (5′-CCAGCA(G/C)C(C/T)GCGGTAATTCC-3'), TAReukREV3 (5′-ACTTTCGTTCTTGAT(C/T)(A/G)A-3′) ph (topsoil) 5.9 sample collection device or method Each site was sampled on 3 points located at 10 meters from each other to have 3 biological replicates. Each replicate was composed of four locations taken on the edges of a 10 cm square. At each location, the organic layer and the top 5 cm of mineral soil were sampled separately. About 100 mL of soil was collected for each replicate in a sterile Ziploc plastic bag, attempting not to disturb the internal sEach site was sampled on 3 points located at 10 meters from each other to have 3 biological replicates. Each replicate was composed of four locations taken on the edges of a 10 cm square. At each location, the organic layer and the top 5 cm of mineral soil were sampled separately. About 100 mL of soil was collected for each replicate in a sterile Ziploc plastic bag, attempting not to disturb the internal structure of the sample.tructure of the sample. sample name Rec1-min_S11_L001 site Rec1 soil layer Mineral storage frozen target gene 18S target subfragment V4 treated yes treatment reclamation 1, 6 years before sampling ERS2065967 SAMEA104448025 100272 uncultured eukaryote Sample of reclaimed boreal forest soil (ecotype Rec1 : peat over salvaged mineral soil lying above tailings (after >1m); placed 6 years before sampling), kept at -80°C until sequencing ENA first public 2018-12-19 ENA last update 2017-12-21 External Id SAMEA104448025 INSDC center alias IBENS INSDC center name Institut de Biologie de Ecole normale superieure (IBENS), France INSDC first public 2018-12-19T17:03:06Z INSDC last update 2017-12-21T17:48:35Z INSDC status public Submitter Id Rec1-M2_S7_L001 averaged total c 3.72 averaged total n 0.17 collection date 2013-07-18 environment (biome) taiga (boreal forest) environment (feature) above Albertan bitumen ore deposit environment (material) forest soil environmental sample Yes further details http://unieuk.org/project/ geographic location (country and/or sea) Canada geographic location (latitude) 57.019578 geographic location (longitude) -111.728472 geographic location (region and locality) Northeastern Alberta pcr primers TAReuk454FWD1 (5′-CCAGCA(G/C)C(C/T)GCGGTAATTCC-3'), TAReukREV3 (5′-ACTTTCGTTCTTGAT(C/T)(A/G)A-3′) ph (topsoil) 5.9 sample collection device or method Each site was sampled on 3 points located at 10 meters from each other to have 3 biological replicates. Each replicate was composed of four locations taken on the edges of a 10 cm square. At each location, the organic layer and the top 5 cm of mineral soil were sampled separately. About 100 mL of soil was collected for each replicate in a sterile Ziploc plastic bag, attempting not to disturb the internal sEach site was sampled on 3 points located at 10 meters from each other to have 3 biological replicates. Each replicate was composed of four locations taken on the edges of a 10 cm square. At each location, the organic layer and the top 5 cm of mineral soil were sampled separately. About 100 mL of soil was collected for each replicate in a sterile Ziploc plastic bag, attempting not to disturb the internal structure of the sample.tructure of the sample. sample name Rec1-M2_S7_L001 site Rec1 soil layer Mineral storage frozen target gene 18S target subfragment V4 treated yes treatment reclamation 1, 6 years before sampling ERS2065968 SAMEA104448026 100272 uncultured eukaryote Sample of reclaimed boreal forest soil (ecotype Rec1 : peat over salvaged mineral soil lying above tailings (after >1m); placed 6 years before sampling), kept at -80°C until sequencing ENA first public 2018-12-19 ENA last update 2017-12-21 External Id SAMEA104448026 INSDC center alias IBENS INSDC center name Institut de Biologie de Ecole normale superieure (IBENS), France INSDC first public 2018-12-19T17:03:06Z INSDC last update 2017-12-21T17:48:18Z INSDC status public Submitter Id Rec1-M3_S8_L001 averaged total c 4.71 averaged total n 0.21 collection date 2013-07-18 environment (biome) taiga (boreal forest) environment (feature) above Albertan bitumen ore deposit environment (material) forest soil environmental sample Yes further details http://unieuk.org/project/ geographic location (country and/or sea) Canada geographic location (latitude) 57.019578 geographic location (longitude) -111.728472 geographic location (region and locality) Northeastern Alberta pcr primers TAReuk454FWD1 (5′-CCAGCA(G/C)C(C/T)GCGGTAATTCC-3'), TAReukREV3 (5′-ACTTTCGTTCTTGAT(C/T)(A/G)A-3′) ph (topsoil) 5.9 sample collection device or method Each site was sampled on 3 points located at 10 meters from each other to have 3 biological replicates. Each replicate was composed of four locations taken on the edges of a 10 cm square. At each location, the organic layer and the top 5 cm of mineral soil were sampled separately. About 100 mL of soil was collected for each replicate in a sterile Ziploc plastic bag, attempting not to disturb the internal sEach site was sampled on 3 points located at 10 meters from each other to have 3 biological replicates. Each replicate was composed of four locations taken on the edges of a 10 cm square. At each location, the organic layer and the top 5 cm of mineral soil were sampled separately. About 100 mL of soil was collected for each replicate in a sterile Ziploc plastic bag, attempting not to disturb the internal structure of the sample.tructure of the sample. sample name Rec1-M3_S8_L001 site Rec1 soil layer Mineral storage frozen target gene 18S target subfragment V4 treated yes treatment reclamation 1, 6 years before sampling ERS2065969 SAMEA104448027 100272 uncultured eukaryote Sample of reclaimed boreal forest soil (ecotype Rec2 : peat over salvaged mineral soil lying above overburden (after >1m); placed 15 years before sampling), kept at -80°C until sequencing ENA first public 2018-12-19 ENA last update 2017-12-21 External Id SAMEA104448027 INSDC center alias IBENS INSDC center name Institut de Biologie de Ecole normale superieure (IBENS), France INSDC first public 2018-12-19T17:03:06Z INSDC last update 2017-12-21T17:47:39Z INSDC status public Submitter Id Rec2-org_S10_L001 averaged total c 34.39 averaged total n 1.43 collection date 2013-07-16 environment (biome) taiga (boreal forest) environment (feature) above Albertan bitumen ore deposit environment (material) forest soil environmental sample Yes further details http://unieuk.org/project/ geographic location (country and/or sea) Canada geographic location (latitude) 56.996253 geographic location (longitude) -111.614222 geographic location (region and locality) Northeastern Alberta pcr primers TAReuk454FWD1 (5′-CCAGCA(G/C)C(C/T)GCGGTAATTCC-3'), TAReukREV3 (5′-ACTTTCGTTCTTGAT(C/T)(A/G)A-3′) ph (topsoil) 5.1 sample collection device or method Each site was sampled on 3 points located at 10 meters from each other to have 3 biological replicates. Each replicate was composed of four locations taken on the edges of a 10 cm square. At each location, the organic layer and the top 5 cm of mineral soil were sampled separately. About 100 mL of soil was collected for each replicate in a sterile Ziploc plastic bag, attempting not to disturb the internal sEach site was sampled on 3 points located at 10 meters from each other to have 3 biological replicates. Each replicate was composed of four locations taken on the edges of a 10 cm square. At each location, the organic layer and the top 5 cm of mineral soil were sampled separately. About 100 mL of soil was collected for each replicate in a sterile Ziploc plastic bag, attempting not to disturb the internal structure of the sample.tructure of the sample. sample name Rec2-org_S10_L001 site Rec2 soil layer Organic storage frozen target gene 18S target subfragment V4 treated yes treatment reclamation 2, 15 years before sampling ERS2065970 SAMEA104448028 100272 uncultured eukaryote Sample of reclaimed boreal forest soil (ecotype Rec2 : peat over salvaged mineral soil lying above overburden (after >1m); placed 15 years before sampling), kept at -80°C until sequencing ENA first public 2018-12-19 ENA last update 2017-12-21 External Id SAMEA104448028 INSDC center alias IBENS INSDC center name Institut de Biologie de Ecole normale superieure (IBENS), France INSDC first public 2018-12-19T17:03:06Z INSDC last update 2017-12-21T17:47:05Z INSDC status public Submitter Id Rec2-O2_S1_L001 averaged total c 19.2 averaged total n 1.05 collection date 2013-07-16 environment (biome) taiga (boreal forest) environment (feature) above Albertan bitumen ore deposit environment (material) forest soil environmental sample Yes further details http://unieuk.org/project/ geographic location (country and/or sea) Canada geographic location (latitude) 56.996253 geographic location (longitude) -111.614222 geographic location (region and locality) Northeastern Alberta pcr primers TAReuk454FWD1 (5′-CCAGCA(G/C)C(C/T)GCGGTAATTCC-3'), TAReukREV3 (5′-ACTTTCGTTCTTGAT(C/T)(A/G)A-3′) ph (topsoil) 5.1 sample collection device or method Each site was sampled on 3 points located at 10 meters from each other to have 3 biological replicates. Each replicate was composed of four locations taken on the edges of a 10 cm square. At each location, the organic layer and the top 5 cm of mineral soil were sampled separately. About 100 mL of soil was collected for each replicate in a sterile Ziploc plastic bag, attempting not to disturb the internal sEach site was sampled on 3 points located at 10 meters from each other to have 3 biological replicates. Each replicate was composed of four locations taken on the edges of a 10 cm square. At each location, the organic layer and the top 5 cm of mineral soil were sampled separately. About 100 mL of soil was collected for each replicate in a sterile Ziploc plastic bag, attempting not to disturb the internal structure of the sample.tructure of the sample. sample name Rec2-O2_S1_L001 site Rec2 soil layer Organic storage frozen target gene 18S target subfragment V4 treated yes treatment reclamation 2, 15 years before sampling ERS2065971 SAMEA104448029 100272 uncultured eukaryote Sample of reclaimed boreal forest soil (ecotype Rec2 : peat over salvaged mineral soil lying above overburden (after >1m); placed 15 years before sampling), kept at -80°C until sequencing ENA first public 2018-12-19 ENA last update 2017-12-21 External Id SAMEA104448029 INSDC center alias IBENS INSDC center name Institut de Biologie de Ecole normale superieure (IBENS), France INSDC first public 2018-12-19T17:03:06Z INSDC last update 2017-12-21T17:46:45Z INSDC status public Submitter Id Rec2-O3_S2_L001 averaged total c 23.61 averaged total n 1.07 collection date 2013-07-16 environment (biome) taiga (boreal forest) environment (feature) above Albertan bitumen ore deposit environment (material) forest soil environmental sample Yes further details http://unieuk.org/project/ geographic location (country and/or sea) Canada geographic location (latitude) 56.996253 geographic location (longitude) -111.614222 geographic location (region and locality) Northeastern Alberta pcr primers TAReuk454FWD1 (5′-CCAGCA(G/C)C(C/T)GCGGTAATTCC-3'), TAReukREV3 (5′-ACTTTCGTTCTTGAT(C/T)(A/G)A-3′) ph (topsoil) 5.1 sample collection device or method Each site was sampled on 3 points located at 10 meters from each other to have 3 biological replicates. Each replicate was composed of four locations taken on the edges of a 10 cm square. At each location, the organic layer and the top 5 cm of mineral soil were sampled separately. About 100 mL of soil was collected for each replicate in a sterile Ziploc plastic bag, attempting not to disturb the internal sEach site was sampled on 3 points located at 10 meters from each other to have 3 biological replicates. Each replicate was composed of four locations taken on the edges of a 10 cm square. At each location, the organic layer and the top 5 cm of mineral soil were sampled separately. About 100 mL of soil was collected for each replicate in a sterile Ziploc plastic bag, attempting not to disturb the internal structure of the sample.tructure of the sample. sample name Rec2-O3_S2_L001 site Rec2 soil layer Organic storage frozen target gene 18S target subfragment V4 treated yes treatment reclamation 2, 15 years before sampling ERS2065972 SAMEA104448030 100272 uncultured eukaryote Sample of reclaimed boreal forest soil (ecotype Rec2 : peat over salvaged mineral soil lying above overburden (after >1m); placed 15 years before sampling), kept at -80°C until sequencing ENA first public 2018-12-19 ENA last update 2017-12-21 External Id SAMEA104448030 INSDC center alias IBENS INSDC center name Institut de Biologie de Ecole normale superieure (IBENS), France INSDC first public 2018-12-19T17:03:06Z INSDC last update 2017-12-21T17:46:24Z INSDC status public Submitter Id Rec2-min_S9_L001 averaged total c 36.57 averaged total n 1.39 collection date 2013-07-16 environment (biome) taiga (boreal forest) environment (feature) above Albertan bitumen ore deposit environment (material) forest soil environmental sample Yes further details http://unieuk.org/project/ geographic location (country and/or sea) Canada geographic location (latitude) 56.996253 geographic location (longitude) -111.614222 geographic location (region and locality) Northeastern Alberta pcr primers TAReuk454FWD1 (5′-CCAGCA(G/C)C(C/T)GCGGTAATTCC-3'), TAReukREV3 (5′-ACTTTCGTTCTTGAT(C/T)(A/G)A-3′) ph (topsoil) 5.1 sample collection device or method Each site was sampled on 3 points located at 10 meters from each other to have 3 biological replicates. Each replicate was composed of four locations taken on the edges of a 10 cm square. At each location, the organic layer and the top 5 cm of mineral soil were sampled separately. About 100 mL of soil was collected for each replicate in a sterile Ziploc plastic bag, attempting not to disturb the internal sEach site was sampled on 3 points located at 10 meters from each other to have 3 biological replicates. Each replicate was composed of four locations taken on the edges of a 10 cm square. At each location, the organic layer and the top 5 cm of mineral soil were sampled separately. About 100 mL of soil was collected for each replicate in a sterile Ziploc plastic bag, attempting not to disturb the internal structure of the sample.tructure of the sample. sample name Rec2-min_S9_L001 site Rec2 soil layer Mineral storage frozen target gene 18S target subfragment V4 treated yes treatment reclamation 2, 15 years before sampling ERS2065973 SAMEA104448031 100272 uncultured eukaryote Sample of reclaimed boreal forest soil (ecotype Rec2 : peat over salvaged mineral soil lying above overburden (after >1m); placed 15 years before sampling), kept at -80°C until sequencing ENA first public 2018-12-19 ENA last update 2017-12-21 External Id SAMEA104448031 INSDC center alias IBENS INSDC center name Institut de Biologie de Ecole normale superieure (IBENS), France INSDC first public 2018-12-19T17:03:06Z INSDC last update 2017-12-21T17:46:04Z INSDC status public Submitter Id Rec2-M2_S3_L001 averaged total c 27.67 averaged total n 0.99 collection date 2013-07-16 environment (biome) taiga (boreal forest) environment (feature) above Albertan bitumen ore deposit environment (material) forest soil environmental sample Yes further details http://unieuk.org/project/ geographic location (country and/or sea) Canada geographic location (latitude) 56.996253 geographic location (longitude) -111.614222 geographic location (region and locality) Northeastern Alberta pcr primers TAReuk454FWD1 (5′-CCAGCA(G/C)C(C/T)GCGGTAATTCC-3'), TAReukREV3 (5′-ACTTTCGTTCTTGAT(C/T)(A/G)A-3′) ph (topsoil) 5.1 sample collection device or method Each site was sampled on 3 points located at 10 meters from each other to have 3 biological replicates. Each replicate was composed of four locations taken on the edges of a 10 cm square. At each location, the organic layer and the top 5 cm of mineral soil were sampled separately. About 100 mL of soil was collected for each replicate in a sterile Ziploc plastic bag, attempting not to disturb the internal sEach site was sampled on 3 points located at 10 meters from each other to have 3 biological replicates. Each replicate was composed of four locations taken on the edges of a 10 cm square. At each location, the organic layer and the top 5 cm of mineral soil were sampled separately. About 100 mL of soil was collected for each replicate in a sterile Ziploc plastic bag, attempting not to disturb the internal structure of the sample.tructure of the sample. sample name Rec2-M2_S3_L001 site Rec2 soil layer Mineral storage frozen target gene 18S target subfragment V4 treated yes treatment reclamation 2, 15 years before sampling ERS2065974 SAMEA104448032 100272 uncultured eukaryote Sample of reclaimed boreal forest soil (ecotype Rec2 : peat over salvaged mineral soil lying above overburden (after >1m); placed 15 years before sampling), kept at -80°C until sequencing ENA first public 2018-12-19 ENA last update 2017-12-21 External Id SAMEA104448032 INSDC center alias IBENS INSDC center name Institut de Biologie de Ecole normale superieure (IBENS), France INSDC first public 2018-12-19T17:03:06Z INSDC last update 2017-12-21T17:44:13Z INSDC status public Submitter Id Rec2-M3_S4_L001 averaged total c 30.37 averaged total n 1.08 collection date 2013-07-16 environment (biome) taiga (boreal forest) environment (feature) above Albertan bitumen ore deposit environment (material) forest soil environmental sample Yes further details http://unieuk.org/project/ geographic location (country and/or sea) Canada geographic location (latitude) 56.996253 geographic location (longitude) -111.614222 geographic location (region and locality) Northeastern Alberta pcr primers TAReuk454FWD1 (5′-CCAGCA(G/C)C(C/T)GCGGTAATTCC-3'), TAReukREV3 (5′-ACTTTCGTTCTTGAT(C/T)(A/G)A-3′) ph (topsoil) 5.1 sample collection device or method Each site was sampled on 3 points located at 10 meters from each other to have 3 biological replicates. Each replicate was composed of four locations taken on the edges of a 10 cm square. At each location, the organic layer and the top 5 cm of mineral soil were sampled separately. About 100 mL of soil was collected for each replicate in a sterile Ziploc plastic bag, attempting not to disturb the internal sEach site was sampled on 3 points located at 10 meters from each other to have 3 biological replicates. Each replicate was composed of four locations taken on the edges of a 10 cm square. At each location, the organic layer and the top 5 cm of mineral soil were sampled separately. About 100 mL of soil was collected for each replicate in a sterile Ziploc plastic bag, attempting not to disturb the internal structure of the sample.tructure of the sample. sample name Rec2-M3_S4_L001 site Rec2 soil layer Mineral storage frozen target gene 18S target subfragment V4 treated yes treatment reclamation 2, 15 years before sampling