ERP105998 PRJEB24189 ena-STUDY-Fish immunology, department of biology, University of Beren-04-01-2018-12:52:04:749-667 Transcriptome-wide mapping of early immune responses in lumpfish We performed RNA sequencing, identified components of the immune system and mapped early immune responses of lumpfish (Cyclopterus lumpus) leukocytes following in vitro bacterial exposure to the pathogenic bacterium Vibrio anguillarum. and mapped pattern recognition receptors, signaling pathways and innate immune responses. This is the first characterization of immune molecules in lumpfish at gene level. In silico analyses showed that genes encoding proteins involved in pathogen recognition, cell signaling and cytokines in higher organisms were conserved in lumpfish, and also identified some unique molecules. Pathogen recognition components includes 13 TLRs, 12 NLRs and several complement factors. We identified TLR1, -2, -3, -5 (membrane-bound and soluble), -7, -8, -9, -13, -14, -21, -22 and -28. The phylogeny of currently recognized TLRs has thus been revised. Transcriptome-wide analyses of immune responses 6 and 24 hours post bacterial exposure revealed differential expression of 9033 and 15225 genes, respectively. These included TLR5S, IL-1ß, IL-8, IL-6, TNFa, IL-17A/F3 and IL-17C. The data generated will be valuable for comparative studies and will contribute to determination of the phylogenetic position of the Cyclopteridae, which is currently debated in the literature. It will also make an important basis for further functional analyses of immune and pathogenicity mechanisms. Such knowledge is also important for design of immunoprophylactic measures in lumpfish, a species of fish now farmed intensively for use as cleaner-fish in Atlantic salmon (Salmo salar) farming. Transcriptome-wide mapping of signaling pathways and early immune responses in lumpfish leukocytes upon in vitro bacterial exposure We performed RNA sequencing, identified components of the immune system and mapped early immune responses of lumpfish (Cyclopterus lumpus) leukocytes following in vitro bacterial exposure to the pathogenic bacterium Vibrio anguillarum. and mapped pattern recognition receptors, signaling pathways and innate immune responses. This is the first characterization of immune molecules in lumpfish at gene level. In silico analyses showed that genes encoding proteins involved in pathogen recognition, cell signaling and cytokines in higher organisms were conserved in lumpfish, and also identified some unique molecules. Pathogen recognition components includes 13 TLRs, 12 NLRs and several complement factors. We identified TLR1, -2, -3, -5 (membrane-bound and soluble), -7, -8, -9, -13, -14, -21, -22 and -28. The phylogeny of currently recognized TLRs has thus been revised. Transcriptome-wide analyses of immune responses 6 and 24 hours post bacterial exposure revealed differential expression of 9033 and 15225 genes, respectively. These included TLR5S, IL-1ß, IL-8, IL-6, TNFa, IL-17A/F3 and IL-17C. The data generated will be valuable for comparative studies and will contribute to determination of the phylogenetic position of the Cyclopteridae, which is currently debated in the literature. It will also make an important basis for further functional analyses of immune and pathogenicity mechanisms. Such knowledge is also important for design of immunoprophylactic measures in lumpfish, a species of fish now farmed intensively for use as cleaner-fish in Atlantic salmon (Salmo salar) farming. ENA-FIRST-PUBLIC 2018-03-03 ENA-LAST-UPDATE 2018-01-04