ERP137177 PRJEB52452 c2553fec-84b0-43d0-816f-42f467e42e53 Malaspina Expedition 2010 Microbial Vertical Profiles Metagenomes Here we present a dataset of 76 microbial metagenomes of the picoplankton size fraction (0.2-3.0 µm) collected in 11 stations along the Malaspina Expedition circumnavigation (http://www.expedicionmalaspina.es; Duarte 2015) that cover vertical profiles sampled at 7 depths, from the surface to 4,000 m deep (or the sea floor in shallower waters), plus 5 additional metagenomes. This Malaspina Microbial Vertical Profiles metagenomes (MProfile) dataset produced 1.66 Tbp of raw DNA sequences. Six L seawater samples were collected and filtered through a 200 and a 20 µm mesh to remove large plankton, the prokarotic free-living size fraction (0.2 to 3.0 µm) was recovered in each sample by pumping water serially through 47-mm polycarbonate membrane filters of 3.0 µm and 0.22 µm pore sizes with a peristaltic pump. DNA was extracted using a standard phenol-chloroform protocol and was sequenced at the National Center for Genomic Analsis (CNAG-CRG. Barcelona, Spain; www.cnag.es), funded by project MALASPINOMICS (CTM2011-15461-E, led by Carlos M Duarte) on the Illumina HiSeq2000 sequencing platform utilizing a TruSeq paired-end cluster kit, v3, following a 2x100 indexed run recipe. References: Duarte CM. 2015. Seafaring in the 21St Century: The Malaspina 2010 Circumnavigation Expedition. Limnology and Oceanography Bulletin 24:11-14; Sánchez, P. et al., 2023. Marine picoplankton metagenomes from eleven vertical profiles obtained by the Malaspina Expedition in the tropical and subtropical oceans. bioRxiv 2023.02.06.526790; doi: https://doi.org/10.1101/2023.02.06.526790 Malaspina Microbial Vertical Profiles Metagenomes Here we present a dataset of 76 microbial metagenomes of the picoplankton size fraction (0.2-3.0 µm) collected in 11 stations along the Malaspina Expedition circumnavigation (http://www.expedicionmalaspina.es; Duarte 2015) that cover vertical profiles sampled at 7 depths, from the surface to 4,000 m deep (or the sea floor in shallower waters), plus 5 additional metagenomes. This Malaspina Microbial Vertical Profiles metagenomes (MProfile) dataset produced 1.66 Tbp of raw DNA sequences. Six L seawater samples were collected and filtered through a 200 and a 20 µm mesh to remove large plankton, the prokarotic free-living size fraction (0.2 to 3.0 µm) was recovered in each sample by pumping water serially through 47-mm polycarbonate membrane filters of 3.0 µm and 0.22 µm pore sizes with a peristaltic pump. DNA was extracted using a standard phenol-chloroform protocol and was sequenced at the National Center for Genomic Analsis (CNAG-CRG. Barcelona, Spain; www.cnag.es), funded by project MALASPINOMICS (CTM2011-15461-E, led by Carlos M Duarte) on the Illumina HiSeq2000 sequencing platform utilizing a TruSeq paired-end cluster kit, v3, following a 2x100 indexed run recipe. References: Duarte CM. 2015. Seafaring in the 21St Century: The Malaspina 2010 Circumnavigation Expedition. Limnology and Oceanography Bulletin 24:11-14; Sánchez, P. et al., 2023. Marine picoplankton metagenomes from eleven vertical profiles obtained by the Malaspina Expedition in the tropical and subtropical oceans. bioRxiv 2023.02.06.526790; doi: https://doi.org/10.1101/2023.02.06.526790 ENA-FIRST-PUBLIC 2023-02-07 ENA-LAST-UPDATE 2023-02-07