ERP108380 PRJEB26394 E-MTAB-6704 Identification of RNase III cleavage sites in Escherichia coli In this study we generated 5'P libraries in wt and RNAse III mutant strains, grown to exponential and stationary phases. Libraries that retain short RNA fragments were also generated in both growth phases. After sequencing by Illumina NextSeq 500 system, reads were mapped to E. coli genome NC_000913.3. By comparing the read start counts per position in the wt and mutant strain libraries we identified the cleavage sites of RNase III. In this study we generated 5'P libraries in wt and RNAse III mutant strains, grown to exponential and stationary phases. Libraries that retain short RNA fragments were also generated in both growth phases. After sequencing by Illumina NextSeq 500 system, reads were mapped to E. coli genome NC_000913.3. By comparing the read start counts per position in the wt and mutant strain libraries we identified the cleavage sites of RNase III. E-MTAB-6704 in ArrayExpress http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-6704 ENA-FIRST-PUBLIC 2018-09-30 ENA-LAST-UPDATE 2018-04-24