ERS2429868 SAMEA4609174 408172 marine metagenome Levantine Sea (Eastern Mediterranean); plankton; eukaryotic and prokaryotic communities composition; amplicons; illumina MiSeq; size fractionation by filtration DNA extraction method PowerWater DNA isolation kit ENA-FIRST-PUBLIC 2019-06-23T04:04:02Z ENA-LAST-UPDATE 2018-04-25T07:47:41Z External Id SAMEA4609174 INSDC center name HELLENIC CENTRE FOR MARINE RESEARCH INSDC first public 2019-06-23T04:04:02Z INSDC last update 2018-04-25T07:47:41Z INSDC status public Latitude End 35.0333 Longitude End 23.4667 Submitter Id LV_1 amount or size of sample collected 29 depth 3300 environment (biome) temperate mediterannean sea biome environment (feature) marine pelagic feature environment (material) planktonic material environmental package water event date/time 2016-04-17T07:45:00Z fraction_microns 20 latitude start 35.0333 longitude start 23.4667 marine region Levantine basin (eastern Mediterannean) primer_f 5'CCCTGCCHTTTGTACACAC primer_f_reference Amaral-Zettler LA, McCliment EA, Ducklow HW, Huse SM. (2009). A Method for Studying Protistan Diversity Using Massively Parallel Sequencing of V9 Hypervariable Regions of Small-Subunit Ribosomal RNA Genes. PLoS One 4: e6372. primer_r 5'CCTTCYGCAGGTTCACCTAC primer_r_reference Amaral-Zettler LA, McCliment EA, Ducklow HW, Huse SM. (2009). A Method for Studying Protistan Diversity Using Massively Parallel Sequencing of V9 Hypervariable Regions of Small-Subunit Ribosomal RNA Genes. PLoS One 4: e6372. project name LEVECO protocol label sequencial filtration and preservation salinity 38.735 sample collection device or method sequencial filtration using peristaltic pump sample name LV_1 sample storage duration 3 sample storage temperature -80 sampling campaign cruise sampling platform Research Vessel HCMR sampling site LV_st_3 scientific_name marine metagenome target gene 18S temperature 13 total depth of water column 3584 ERS2429869 SAMEA4609175 408172 marine metagenome Levantine Sea (Eastern Mediterranean); plankton; eukaryotic and prokaryotic communities composition; amplicons; illumina MiSeq; size fractionation by filtration DNA extraction method PowerWater DNA isolation kit ENA-FIRST-PUBLIC 2019-06-23T04:04:02Z ENA-LAST-UPDATE 2018-04-25T07:47:42Z External Id SAMEA4609175 INSDC center name HELLENIC CENTRE FOR MARINE RESEARCH INSDC first public 2019-06-23T04:04:02Z INSDC last update 2018-04-25T07:47:42Z INSDC status public Latitude End 35.0333 Longitude End 23.4667 Submitter Id LV_2 amount or size of sample collected 29 depth 3300 environment (biome) temperate mediterannean sea biome environment (feature) marine pelagic feature environment (material) planktonic material environmental package water event date/time 2016-04-17T07:45:00Z fraction_microns 0.8 latitude start 35.0333 longitude start 23.4667 marine region Levantine basin (eastern Mediterannean) primer_f 5'CCCTGCCHTTTGTACACAC primer_f_reference Amaral-Zettler LA, McCliment EA, Ducklow HW, Huse SM. (2009). A Method for Studying Protistan Diversity Using Massively Parallel Sequencing of V9 Hypervariable Regions of Small-Subunit Ribosomal RNA Genes. PLoS One 4: e6372. primer_r 5'CCTTCYGCAGGTTCACCTAC primer_r_reference Amaral-Zettler LA, McCliment EA, Ducklow HW, Huse SM. (2009). A Method for Studying Protistan Diversity Using Massively Parallel Sequencing of V9 Hypervariable Regions of Small-Subunit Ribosomal RNA Genes. PLoS One 4: e6372. project name LEVECO protocol label sequencial filtration and preservation salinity 38.735 sample collection device or method sequencial filtration using peristaltic pump sample name LV_2 sample storage duration 3 sample storage temperature -80 sampling campaign cruise sampling platform Research Vessel HCMR sampling site LV_st_3 scientific_name marine metagenome target gene 18S temperature 13 total depth of water column 3584 ERS2429871 SAMEA4609177 408172 marine metagenome Levantine Sea (Eastern Mediterranean); plankton; eukaryotic and prokaryotic communities composition; amplicons; illumina MiSeq; size fractionation by filtration DNA extraction method PowerWater DNA isolation kit ENA-FIRST-PUBLIC 2019-06-23T04:04:02Z ENA-LAST-UPDATE 2018-04-25T07:47:42Z External Id SAMEA4609177 INSDC center name HELLENIC CENTRE FOR MARINE RESEARCH INSDC first public 2019-06-23T04:04:02Z INSDC last update 2018-04-25T07:47:42Z INSDC status public Latitude End 35.0333 Longitude End 23.4667 Submitter Id LV_4 amount or size of sample collected 18.5 depth 5 environment (biome) temperate mediterannean sea biome environment (feature) marine pelagic feature environment (material) planktonic material environmental package water event date/time 2016-04-17T12:00:00Z fraction_microns 20 latitude start 35.0333 longitude start 23.4667 marine region Levantine basin (eastern Mediterannean) primer_f 5'CCCTGCCHTTTGTACACAC primer_f_reference Amaral-Zettler LA, McCliment EA, Ducklow HW, Huse SM. (2009). A Method for Studying Protistan Diversity Using Massively Parallel Sequencing of V9 Hypervariable Regions of Small-Subunit Ribosomal RNA Genes. PLoS One 4: e6372. primer_r 5'CCTTCYGCAGGTTCACCTAC primer_r_reference Amaral-Zettler LA, McCliment EA, Ducklow HW, Huse SM. (2009). A Method for Studying Protistan Diversity Using Massively Parallel Sequencing of V9 Hypervariable Regions of Small-Subunit Ribosomal RNA Genes. PLoS One 4: e6372. project name LEVECO protocol label sequencial filtration and preservation salinity 38.95 sample collection device or method sequencial filtration using peristaltic pump sample name LV_4 sample storage duration 3 sample storage temperature -80 sampling campaign cruise sampling platform Research Vessel HCMR sampling site LV_st_3 scientific_name marine metagenome target gene 18S temperature 17.5 total depth of water column 3584 ERS2429872 SAMEA4609178 408172 marine metagenome Levantine Sea (Eastern Mediterranean); plankton; eukaryotic and prokaryotic communities composition; amplicons; illumina MiSeq; size fractionation by filtration DNA extraction method PowerWater DNA isolation kit ENA-FIRST-PUBLIC 2019-06-23T04:04:02Z ENA-LAST-UPDATE 2018-04-25T07:47:42Z External Id SAMEA4609178 INSDC center name HELLENIC CENTRE FOR MARINE RESEARCH INSDC first public 2019-06-23T04:04:02Z INSDC last update 2018-04-25T07:47:42Z INSDC status public Latitude End 35.0333 Longitude End 23.4667 Submitter Id LV_5 amount or size of sample collected 18.5 depth 5 environment (biome) temperate mediterannean sea biome environment (feature) marine pelagic feature environment (material) planktonic material environmental package water event date/time 2016-04-17T12:00:00Z fraction_microns 0.8 latitude start 35.0333 longitude start 23.4667 marine region Levantine basin (eastern Mediterannean) primer_f 5'CCCTGCCHTTTGTACACAC primer_f_reference Amaral-Zettler LA, McCliment EA, Ducklow HW, Huse SM. (2009). A Method for Studying Protistan Diversity Using Massively Parallel Sequencing of V9 Hypervariable Regions of Small-Subunit Ribosomal RNA Genes. PLoS One 4: e6372. primer_r 5'CCTTCYGCAGGTTCACCTAC primer_r_reference Amaral-Zettler LA, McCliment EA, Ducklow HW, Huse SM. (2009). A Method for Studying Protistan Diversity Using Massively Parallel Sequencing of V9 Hypervariable Regions of Small-Subunit Ribosomal RNA Genes. PLoS One 4: e6372. project name LEVECO protocol label sequencial filtration and preservation salinity 38.95 sample collection device or method sequencial filtration using peristaltic pump sample name LV_5 sample storage duration 3 sample storage temperature -80 sampling campaign cruise sampling platform Research Vessel HCMR sampling site LV_st_3 scientific_name marine metagenome target gene 18S temperature 17.5 total depth of water column 3584 ERS2429874 SAMEA4609180 408172 marine metagenome Levantine Sea (Eastern Mediterranean); plankton; eukaryotic and prokaryotic communities composition; amplicons; illumina MiSeq; size fractionation by filtration DNA extraction method PowerWater DNA isolation kit ENA-FIRST-PUBLIC 2019-06-23T04:04:02Z ENA-LAST-UPDATE 2018-04-25T07:47:42Z External Id SAMEA4609180 INSDC center name HELLENIC CENTRE FOR MARINE RESEARCH INSDC first public 2019-06-23T04:04:02Z INSDC last update 2018-04-25T07:47:42Z INSDC status public Latitude End 35.0333 Longitude End 23.4667 Submitter Id LV_7 amount or size of sample collected 18.5 depth 75 environment (biome) temperate mediterannean sea biome environment (feature) marine pelagic feature environment (material) planktonic material environmental package water event date/time 2016-04-17T12:00:00Z fraction_microns 20 latitude start 35.0333 longitude start 23.4667 marine region Levantine basin (eastern Mediterannean) primer_f 5'CCCTGCCHTTTGTACACAC primer_f_reference Amaral-Zettler LA, McCliment EA, Ducklow HW, Huse SM. (2009). A Method for Studying Protistan Diversity Using Massively Parallel Sequencing of V9 Hypervariable Regions of Small-Subunit Ribosomal RNA Genes. PLoS One 4: e6372. primer_r 5'CCTTCYGCAGGTTCACCTAC primer_r_reference Amaral-Zettler LA, McCliment EA, Ducklow HW, Huse SM. (2009). A Method for Studying Protistan Diversity Using Massively Parallel Sequencing of V9 Hypervariable Regions of Small-Subunit Ribosomal RNA Genes. PLoS One 4: e6372. project name LEVECO protocol label sequencial filtration and preservation salinity 39 sample collection device or method sequencial filtration using peristaltic pump sample name LV_7 sample storage duration 3 sample storage temperature -80 sampling campaign cruise sampling platform Research Vessel HCMR sampling site LV_st_3 scientific_name marine metagenome target gene 18S temperature 16.5 total depth of water column 3584 ERS2429875 SAMEA4609181 408172 marine metagenome Levantine Sea (Eastern Mediterranean); plankton; eukaryotic and prokaryotic communities composition; amplicons; illumina MiSeq; size fractionation by filtration DNA extraction method PowerWater DNA isolation kit ENA-FIRST-PUBLIC 2019-06-23T04:04:02Z ENA-LAST-UPDATE 2018-04-25T07:47:42Z External Id SAMEA4609181 INSDC center name HELLENIC CENTRE FOR MARINE RESEARCH INSDC first public 2019-06-23T04:04:02Z INSDC last update 2018-04-25T07:47:42Z INSDC status public Latitude End 35.0333 Longitude End 23.4667 Submitter Id LV_8 amount or size of sample collected 18.5 depth 75 environment (biome) temperate mediterannean sea biome environment (feature) marine pelagic feature environment (material) planktonic material environmental package water event date/time 2016-04-17T12:00:00Z fraction_microns 0.8 latitude start 35.0333 longitude start 23.4667 marine region Levantine basin (eastern Mediterannean) primer_f 5'CCCTGCCHTTTGTACACAC primer_f_reference Amaral-Zettler LA, McCliment EA, Ducklow HW, Huse SM. (2009). A Method for Studying Protistan Diversity Using Massively Parallel Sequencing of V9 Hypervariable Regions of Small-Subunit Ribosomal RNA Genes. PLoS One 4: e6372. primer_r 5'CCTTCYGCAGGTTCACCTAC primer_r_reference Amaral-Zettler LA, McCliment EA, Ducklow HW, Huse SM. (2009). A Method for Studying Protistan Diversity Using Massively Parallel Sequencing of V9 Hypervariable Regions of Small-Subunit Ribosomal RNA Genes. PLoS One 4: e6372. project name LEVECO protocol label sequencial filtration and preservation salinity 39 sample collection device or method sequencial filtration using peristaltic pump sample name LV_8 sample storage duration 3 sample storage temperature -80 sampling campaign cruise sampling platform Research Vessel HCMR sampling site LV_st_3 scientific_name marine metagenome target gene 18S temperature 16.5 total depth of water column 3584 ERS2429877 SAMEA4609183 408172 marine metagenome Levantine Sea (Eastern Mediterranean); plankton; eukaryotic and prokaryotic communities composition; amplicons; illumina MiSeq; size fractionation by filtration DNA extraction method PowerWater DNA isolation kit ENA-FIRST-PUBLIC 2019-06-23T04:04:02Z ENA-LAST-UPDATE 2018-04-25T07:47:42Z External Id SAMEA4609183 INSDC center name HELLENIC CENTRE FOR MARINE RESEARCH INSDC first public 2019-06-23T04:04:02Z INSDC last update 2018-04-25T07:47:42Z INSDC status public Latitude End 35.0333 Longitude End 23.4667 Submitter Id LV_10 amount or size of sample collected 18.5 depth 50 environment (biome) temperate mediterannean sea biome environment (feature) marine pelagic feature environment (material) planktonic material environmental package water event date/time 2016-04-17T12:00:00Z fraction_microns 20 latitude start 35.0333 longitude start 23.4667 marine region Levantine basin (eastern Mediterannean) primer_f 5'CCCTGCCHTTTGTACACAC primer_f_reference Amaral-Zettler LA, McCliment EA, Ducklow HW, Huse SM. (2009). A Method for Studying Protistan Diversity Using Massively Parallel Sequencing of V9 Hypervariable Regions of Small-Subunit Ribosomal RNA Genes. PLoS One 4: e6372. primer_r 5'CCTTCYGCAGGTTCACCTAC primer_r_reference Amaral-Zettler LA, McCliment EA, Ducklow HW, Huse SM. (2009). A Method for Studying Protistan Diversity Using Massively Parallel Sequencing of V9 Hypervariable Regions of Small-Subunit Ribosomal RNA Genes. PLoS One 4: e6372. project name LEVECO protocol label sequencial filtration and preservation salinity 38.95 sample collection device or method sequencial filtration using peristaltic pump sample name LV_10 sample storage duration 3 sample storage temperature -80 sampling campaign cruise sampling platform Research Vessel HCMR sampling site LV_st_3 scientific_name marine metagenome target gene 18S temperature 17 total depth of water column 3584 ERS2429878 SAMEA4609184 408172 marine metagenome Levantine Sea (Eastern Mediterranean); plankton; eukaryotic and prokaryotic communities composition; amplicons; illumina MiSeq; size fractionation by filtration DNA extraction method PowerWater DNA isolation kit ENA-FIRST-PUBLIC 2019-06-23T04:04:02Z ENA-LAST-UPDATE 2018-04-25T07:47:42Z External Id SAMEA4609184 INSDC center name HELLENIC CENTRE FOR MARINE RESEARCH INSDC first public 2019-06-23T04:04:02Z INSDC last update 2018-04-25T07:47:42Z INSDC status public Latitude End 35.0333 Longitude End 23.4667 Submitter Id LV_11 amount or size of sample collected 18.5 depth 50 environment (biome) temperate mediterannean sea biome environment (feature) marine pelagic feature environment (material) planktonic material environmental package water event date/time 2016-04-17T12:00:00Z fraction_microns 0.8 latitude start 35.0333 longitude start 23.4667 marine region Levantine basin (eastern Mediterannean) primer_f 5'CCCTGCCHTTTGTACACAC primer_f_reference Amaral-Zettler LA, McCliment EA, Ducklow HW, Huse SM. (2009). A Method for Studying Protistan Diversity Using Massively Parallel Sequencing of V9 Hypervariable Regions of Small-Subunit Ribosomal RNA Genes. PLoS One 4: e6372. primer_r 5'CCTTCYGCAGGTTCACCTAC primer_r_reference Amaral-Zettler LA, McCliment EA, Ducklow HW, Huse SM. (2009). A Method for Studying Protistan Diversity Using Massively Parallel Sequencing of V9 Hypervariable Regions of Small-Subunit Ribosomal RNA Genes. PLoS One 4: e6372. project name LEVECO protocol label sequencial filtration and preservation salinity 38.95 sample collection device or method sequencial filtration using peristaltic pump sample name LV_11 sample storage duration 3 sample storage temperature -80 sampling campaign cruise sampling platform Research Vessel HCMR sampling site LV_st_3 scientific_name marine metagenome target gene 18S temperature 17 total depth of water column 3584 ERS2429880 SAMEA4609186 408172 marine metagenome Levantine Sea (Eastern Mediterranean); plankton; eukaryotic and prokaryotic communities composition; amplicons; illumina MiSeq; size fractionation by filtration DNA extraction method PowerWater DNA isolation kit ENA-FIRST-PUBLIC 2019-06-23T04:04:02Z ENA-LAST-UPDATE 2018-04-25T07:47:42Z External Id SAMEA4609186 INSDC center name HELLENIC CENTRE FOR MARINE RESEARCH INSDC first public 2019-06-23T04:04:02Z INSDC last update 2018-04-25T07:47:42Z INSDC status public Latitude End 35.0333 Longitude End 23.4667 Submitter Id LV_13 amount or size of sample collected 25.8 depth 1000 environment (biome) temperate mediterannean sea biome environment (feature) marine pelagic feature environment (material) planktonic material environmental package water event date/time 2016-04-17T16:45:00Z fraction_microns 20 latitude start 35.0333 longitude start 23.4667 marine region Levantine basin (eastern Mediterannean) primer_f 5'CCCTGCCHTTTGTACACAC primer_f_reference Amaral-Zettler LA, McCliment EA, Ducklow HW, Huse SM. (2009). A Method for Studying Protistan Diversity Using Massively Parallel Sequencing of V9 Hypervariable Regions of Small-Subunit Ribosomal RNA Genes. PLoS One 4: e6372. primer_r 5'CCTTCYGCAGGTTCACCTAC primer_r_reference Amaral-Zettler LA, McCliment EA, Ducklow HW, Huse SM. (2009). A Method for Studying Protistan Diversity Using Massively Parallel Sequencing of V9 Hypervariable Regions of Small-Subunit Ribosomal RNA Genes. PLoS One 4: e6372. project name LEVECO protocol label sequencial filtration and preservation salinity 38.76 sample collection device or method sequencial filtration using peristaltic pump sample name LV_13 sample storage duration 3 sample storage temperature -80 sampling campaign cruise sampling platform Research Vessel HCMR sampling site LV_st_3 scientific_name marine metagenome target gene 18S temperature 13.6 total depth of water column 3584 ERS2429881 SAMEA4609187 408172 marine metagenome Levantine Sea (Eastern Mediterranean); plankton; eukaryotic and prokaryotic communities composition; amplicons; illumina MiSeq; size fractionation by filtration DNA extraction method PowerWater DNA isolation kit ENA-FIRST-PUBLIC 2019-06-23T04:04:02Z ENA-LAST-UPDATE 2018-04-25T07:47:42Z External Id SAMEA4609187 INSDC center name HELLENIC CENTRE FOR MARINE RESEARCH INSDC first public 2019-06-23T04:04:02Z INSDC last update 2018-04-25T07:47:42Z INSDC status public Latitude End 35.0333 Longitude End 23.4667 Submitter Id LV_14 amount or size of sample collected 25.8 depth 1000 environment (biome) temperate mediterannean sea biome environment (feature) marine pelagic feature environment (material) planktonic material environmental package water event date/time 2016-04-17T16:45:00Z fraction_microns 0.8 latitude start 35.0333 longitude start 23.4667 marine region Levantine basin (eastern Mediterannean) primer_f 5'CCCTGCCHTTTGTACACAC primer_f_reference Amaral-Zettler LA, McCliment EA, Ducklow HW, Huse SM. (2009). A Method for Studying Protistan Diversity Using Massively Parallel Sequencing of V9 Hypervariable Regions of Small-Subunit Ribosomal RNA Genes. PLoS One 4: e6372. primer_r 5'CCTTCYGCAGGTTCACCTAC primer_r_reference Amaral-Zettler LA, McCliment EA, Ducklow HW, Huse SM. (2009). A Method for Studying Protistan Diversity Using Massively Parallel Sequencing of V9 Hypervariable Regions of Small-Subunit Ribosomal RNA Genes. PLoS One 4: e6372. project name LEVECO protocol label sequencial filtration and preservation salinity 38.76 sample collection device or method sequencial filtration using peristaltic pump sample name LV_14 sample storage duration 3 sample storage temperature -80 sampling campaign cruise sampling platform Research Vessel HCMR sampling site LV_st_3 scientific_name marine metagenome target gene 18S temperature 13.6 total depth of water column 3584 ERS2429883 SAMEA4609189 408172 marine metagenome Levantine Sea (Eastern Mediterranean); plankton; eukaryotic and prokaryotic communities composition; amplicons; illumina MiSeq; size fractionation by filtration DNA extraction method PowerWater DNA isolation kit ENA-FIRST-PUBLIC 2019-06-23T04:04:02Z ENA-LAST-UPDATE 2018-04-25T07:47:42Z External Id SAMEA4609189 INSDC center name HELLENIC CENTRE FOR MARINE RESEARCH INSDC first public 2019-06-23T04:04:02Z INSDC last update 2018-04-25T07:47:42Z INSDC status public Latitude End 34.6667 Longitude End 24.3667 Submitter Id LV_16 amount or size of sample collected 30 depth 3200 environment (biome) temperate mediterannean sea biome environment (feature) marine pelagic feature environment (material) planktonic material environmental package water event date/time 2016-04-15T09:20:00Z fraction_microns 20 latitude start 34.6667 longitude start 24.3667 marine region Levantine basin (eastern Mediterannean) primer_f 5'CCCTGCCHTTTGTACACAC primer_f_reference Amaral-Zettler LA, McCliment EA, Ducklow HW, Huse SM. (2009). A Method for Studying Protistan Diversity Using Massively Parallel Sequencing of V9 Hypervariable Regions of Small-Subunit Ribosomal RNA Genes. PLoS One 4: e6372. primer_r 5'CCTTCYGCAGGTTCACCTAC primer_r_reference Amaral-Zettler LA, McCliment EA, Ducklow HW, Huse SM. (2009). A Method for Studying Protistan Diversity Using Massively Parallel Sequencing of V9 Hypervariable Regions of Small-Subunit Ribosomal RNA Genes. PLoS One 4: e6372. project name LEVECO protocol label sequencial filtration and preservation salinity 38.735 sample collection device or method sequencial filtration using peristaltic pump sample name LV_16 sample storage duration 3 sample storage temperature -80 sampling campaign cruise sampling platform Research Vessel HCMR sampling site LV_st_10 scientific_name marine metagenome target gene 18S temperature 13.2 total depth of water column 3400 ERS2429884 SAMEA4609190 408172 marine metagenome Levantine Sea (Eastern Mediterranean); plankton; eukaryotic and prokaryotic communities composition; amplicons; illumina MiSeq; size fractionation by filtration DNA extraction method PowerWater DNA isolation kit ENA-FIRST-PUBLIC 2019-06-23T04:04:02Z ENA-LAST-UPDATE 2018-04-25T07:47:42Z External Id SAMEA4609190 INSDC center name HELLENIC CENTRE FOR MARINE RESEARCH INSDC first public 2019-06-23T04:04:02Z INSDC last update 2018-04-25T07:47:42Z INSDC status public Latitude End 34.6667 Longitude End 24.3667 Submitter Id LV_17 amount or size of sample collected 30 depth 3200 environment (biome) temperate mediterannean sea biome environment (feature) marine pelagic feature environment (material) planktonic material environmental package water event date/time 2016-04-15T09:20:00Z fraction_microns 0.8 latitude start 34.6667 longitude start 24.3667 marine region Levantine basin (eastern Mediterannean) primer_f 5'CCCTGCCHTTTGTACACAC primer_f_reference Amaral-Zettler LA, McCliment EA, Ducklow HW, Huse SM. (2009). A Method for Studying Protistan Diversity Using Massively Parallel Sequencing of V9 Hypervariable Regions of Small-Subunit Ribosomal RNA Genes. PLoS One 4: e6372. primer_r 5'CCTTCYGCAGGTTCACCTAC primer_r_reference Amaral-Zettler LA, McCliment EA, Ducklow HW, Huse SM. (2009). A Method for Studying Protistan Diversity Using Massively Parallel Sequencing of V9 Hypervariable Regions of Small-Subunit Ribosomal RNA Genes. PLoS One 4: e6372. project name LEVECO protocol label sequencial filtration and preservation salinity 38.735 sample collection device or method sequencial filtration using peristaltic pump sample name LV_17 sample storage duration 3 sample storage temperature -80 sampling campaign cruise sampling platform Research Vessel HCMR sampling site LV_st_10 scientific_name marine metagenome target gene 18S temperature 13.2 total depth of water column 3400 ERS2429886 SAMEA4609192 408172 marine metagenome Levantine Sea (Eastern Mediterranean); plankton; eukaryotic and prokaryotic communities composition; amplicons; illumina MiSeq; size fractionation by filtration DNA extraction method PowerWater DNA isolation kit ENA-FIRST-PUBLIC 2019-06-23T04:04:02Z ENA-LAST-UPDATE 2018-04-25T07:47:42Z External Id SAMEA4609192 INSDC center name HELLENIC CENTRE FOR MARINE RESEARCH INSDC first public 2019-06-23T04:04:02Z INSDC last update 2018-04-25T07:47:42Z INSDC status public Latitude End 34.6667 Longitude End 24.3667 Submitter Id LV_19 amount or size of sample collected 20 depth 5 environment (biome) temperate mediterannean sea biome environment (feature) marine pelagic feature environment (material) planktonic material environmental package water event date/time 2016-04-15T10:50:00Z fraction_microns 20 latitude start 34.6667 longitude start 24.3667 marine region Levantine basin (eastern Mediterannean) primer_f 5'CCCTGCCHTTTGTACACAC primer_f_reference Amaral-Zettler LA, McCliment EA, Ducklow HW, Huse SM. (2009). A Method for Studying Protistan Diversity Using Massively Parallel Sequencing of V9 Hypervariable Regions of Small-Subunit Ribosomal RNA Genes. PLoS One 4: e6372. primer_r 5'CCTTCYGCAGGTTCACCTAC primer_r_reference Amaral-Zettler LA, McCliment EA, Ducklow HW, Huse SM. (2009). A Method for Studying Protistan Diversity Using Massively Parallel Sequencing of V9 Hypervariable Regions of Small-Subunit Ribosomal RNA Genes. PLoS One 4: e6372. project name LEVECO protocol label sequencial filtration and preservation salinity 38.9 sample collection device or method sequencial filtration using peristaltic pump sample name LV_19 sample storage duration 3 sample storage temperature -80 sampling campaign cruise sampling platform Research Vessel HCMR sampling site LV_st_10 scientific_name marine metagenome target gene 18S temperature 17.5 total depth of water column 3400 ERS2429887 SAMEA4609193 408172 marine metagenome Levantine Sea (Eastern Mediterranean); plankton; eukaryotic and prokaryotic communities composition; amplicons; illumina MiSeq; size fractionation by filtration DNA extraction method PowerWater DNA isolation kit ENA-FIRST-PUBLIC 2019-06-23T04:04:02Z ENA-LAST-UPDATE 2018-04-25T07:47:42Z External Id SAMEA4609193 INSDC center name HELLENIC CENTRE FOR MARINE RESEARCH INSDC first public 2019-06-23T04:04:02Z INSDC last update 2018-04-25T07:47:42Z INSDC status public Latitude End 34.6667 Longitude End 24.3667 Submitter Id LV_20 amount or size of sample collected 20 depth 5 environment (biome) temperate mediterannean sea biome environment (feature) marine pelagic feature environment (material) planktonic material environmental package water event date/time 2016-04-15T10:50:00Z fraction_microns 0.8 latitude start 34.6667 longitude start 24.3667 marine region Levantine basin (eastern Mediterannean) primer_f 5'CCCTGCCHTTTGTACACAC primer_f_reference Amaral-Zettler LA, McCliment EA, Ducklow HW, Huse SM. (2009). A Method for Studying Protistan Diversity Using Massively Parallel Sequencing of V9 Hypervariable Regions of Small-Subunit Ribosomal RNA Genes. PLoS One 4: e6372. primer_r 5'CCTTCYGCAGGTTCACCTAC primer_r_reference Amaral-Zettler LA, McCliment EA, Ducklow HW, Huse SM. (2009). A Method for Studying Protistan Diversity Using Massively Parallel Sequencing of V9 Hypervariable Regions of Small-Subunit Ribosomal RNA Genes. PLoS One 4: e6372. project name LEVECO protocol label sequencial filtration and preservation salinity 38.9 sample collection device or method sequencial filtration using peristaltic pump sample name LV_20 sample storage duration 3 sample storage temperature -80 sampling campaign cruise sampling platform Research Vessel HCMR sampling site LV_st_10 scientific_name marine metagenome target gene 18S temperature 17.5 total depth of water column 3400 ERS2429889 SAMEA4609195 408172 marine metagenome Levantine Sea (Eastern Mediterranean); plankton; eukaryotic and prokaryotic communities composition; amplicons; illumina MiSeq; size fractionation by filtration DNA extraction method PowerWater DNA isolation kit ENA-FIRST-PUBLIC 2019-06-23T04:04:02Z ENA-LAST-UPDATE 2018-04-25T07:47:42Z External Id SAMEA4609195 INSDC center name HELLENIC CENTRE FOR MARINE RESEARCH INSDC first public 2019-06-23T04:04:02Z INSDC last update 2018-04-25T07:47:42Z INSDC status public Latitude End 34.6667 Longitude End 24.3667 Submitter Id LV_22 amount or size of sample collected 21 depth 75 environment (biome) temperate mediterannean sea biome environment (feature) marine pelagic feature environment (material) planktonic material environmental package water event date/time 2016-04-15T10:50:00Z fraction_microns 20 latitude start 34.6667 longitude start 24.3667 marine region Levantine basin (eastern Mediterannean) primer_f 5'CCCTGCCHTTTGTACACAC primer_f_reference Amaral-Zettler LA, McCliment EA, Ducklow HW, Huse SM. (2009). A Method for Studying Protistan Diversity Using Massively Parallel Sequencing of V9 Hypervariable Regions of Small-Subunit Ribosomal RNA Genes. PLoS One 4: e6372. primer_r 5'CCTTCYGCAGGTTCACCTAC primer_r_reference Amaral-Zettler LA, McCliment EA, Ducklow HW, Huse SM. (2009). A Method for Studying Protistan Diversity Using Massively Parallel Sequencing of V9 Hypervariable Regions of Small-Subunit Ribosomal RNA Genes. PLoS One 4: e6372. project name LEVECO protocol label sequencial filtration and preservation salinity 38.95 sample collection device or method sequencial filtration using peristaltic pump sample name LV_22 sample storage duration 3 sample storage temperature -80 sampling campaign cruise sampling platform Research Vessel HCMR sampling site LV_st_10 scientific_name marine metagenome target gene 18S temperature 16.5 total depth of water column 3400 ERS2429890 SAMEA4609196 408172 marine metagenome Levantine Sea (Eastern Mediterranean); plankton; eukaryotic and prokaryotic communities composition; amplicons; illumina MiSeq; size fractionation by filtration DNA extraction method PowerWater DNA isolation kit ENA-FIRST-PUBLIC 2019-06-23T04:04:02Z ENA-LAST-UPDATE 2018-04-25T07:47:42Z External Id SAMEA4609196 INSDC center name HELLENIC CENTRE FOR MARINE RESEARCH INSDC first public 2019-06-23T04:04:02Z INSDC last update 2018-04-25T07:47:42Z INSDC status public Latitude End 34.6667 Longitude End 24.3667 Submitter Id LV_23 amount or size of sample collected 21 depth 75 environment (biome) temperate mediterannean sea biome environment (feature) marine pelagic feature environment (material) planktonic material environmental package water event date/time 2016-04-15T10:50:00Z fraction_microns 0.8 latitude start 34.6667 longitude start 24.3667 marine region Levantine basin (eastern Mediterannean) primer_f 5'CCCTGCCHTTTGTACACAC primer_f_reference Amaral-Zettler LA, McCliment EA, Ducklow HW, Huse SM. (2009). A Method for Studying Protistan Diversity Using Massively Parallel Sequencing of V9 Hypervariable Regions of Small-Subunit Ribosomal RNA Genes. PLoS One 4: e6372. primer_r 5'CCTTCYGCAGGTTCACCTAC primer_r_reference Amaral-Zettler LA, McCliment EA, Ducklow HW, Huse SM. (2009). A Method for Studying Protistan Diversity Using Massively Parallel Sequencing of V9 Hypervariable Regions of Small-Subunit Ribosomal RNA Genes. PLoS One 4: e6372. project name LEVECO protocol label sequencial filtration and preservation salinity 38.95 sample collection device or method sequencial filtration using peristaltic pump sample name LV_23 sample storage duration 3 sample storage temperature -80 sampling campaign cruise sampling platform Research Vessel HCMR sampling site LV_st_10 scientific_name marine metagenome target gene 18S temperature 16.5 total depth of water column 3400 ERS2429892 SAMEA4609198 408172 marine metagenome Levantine Sea (Eastern Mediterranean); plankton; eukaryotic and prokaryotic communities composition; amplicons; illumina MiSeq; size fractionation by filtration DNA extraction method PowerWater DNA isolation kit ENA-FIRST-PUBLIC 2019-06-23T04:04:02Z ENA-LAST-UPDATE 2018-04-25T07:47:42Z External Id SAMEA4609198 INSDC center name HELLENIC CENTRE FOR MARINE RESEARCH INSDC first public 2019-06-23T04:04:02Z INSDC last update 2018-04-25T07:47:42Z INSDC status public Latitude End 34.6667 Longitude End 24.3667 Submitter Id LV_25 amount or size of sample collected 21 depth 50 environment (biome) temperate mediterannean sea biome environment (feature) marine pelagic feature environment (material) planktonic material environmental package water event date/time 2016-04-15T10:50:00Z fraction_microns 20 latitude start 34.6667 longitude start 24.3667 marine region Levantine basin (eastern Mediterannean) primer_f 5'CCCTGCCHTTTGTACACAC primer_f_reference Amaral-Zettler LA, McCliment EA, Ducklow HW, Huse SM. (2009). A Method for Studying Protistan Diversity Using Massively Parallel Sequencing of V9 Hypervariable Regions of Small-Subunit Ribosomal RNA Genes. PLoS One 4: e6372. primer_r 5'CCTTCYGCAGGTTCACCTAC primer_r_reference Amaral-Zettler LA, McCliment EA, Ducklow HW, Huse SM. (2009). A Method for Studying Protistan Diversity Using Massively Parallel Sequencing of V9 Hypervariable Regions of Small-Subunit Ribosomal RNA Genes. PLoS One 4: e6372. project name LEVECO protocol label sequencial filtration and preservation salinity 38.9 sample collection device or method sequencial filtration using peristaltic pump sample name LV_25 sample storage duration 3 sample storage temperature -80 sampling campaign cruise sampling platform Research Vessel HCMR sampling site LV_st_10 scientific_name marine metagenome target gene 18S temperature 17 total depth of water column 3400 ERS2429893 SAMEA4609199 408172 marine metagenome Levantine Sea (Eastern Mediterranean); plankton; eukaryotic and prokaryotic communities composition; amplicons; illumina MiSeq; size fractionation by filtration DNA extraction method PowerWater DNA isolation kit ENA-FIRST-PUBLIC 2019-06-23T04:04:02Z ENA-LAST-UPDATE 2018-04-25T07:47:42Z External Id SAMEA4609199 INSDC center name HELLENIC CENTRE FOR MARINE RESEARCH INSDC first public 2019-06-23T04:04:02Z INSDC last update 2018-04-25T07:47:42Z INSDC status public Latitude End 34.6667 Longitude End 24.3667 Submitter Id LV_26 amount or size of sample collected 21 depth 50 environment (biome) temperate mediterannean sea biome environment (feature) marine pelagic feature environment (material) planktonic material environmental package water event date/time 2016-04-15T10:50:00Z fraction_microns 0.8 latitude start 34.6667 longitude start 24.3667 marine region Levantine basin (eastern Mediterannean) primer_f 5'CCCTGCCHTTTGTACACAC primer_f_reference Amaral-Zettler LA, McCliment EA, Ducklow HW, Huse SM. (2009). A Method for Studying Protistan Diversity Using Massively Parallel Sequencing of V9 Hypervariable Regions of Small-Subunit Ribosomal RNA Genes. PLoS One 4: e6372. primer_r 5'CCTTCYGCAGGTTCACCTAC primer_r_reference Amaral-Zettler LA, McCliment EA, Ducklow HW, Huse SM. (2009). A Method for Studying Protistan Diversity Using Massively Parallel Sequencing of V9 Hypervariable Regions of Small-Subunit Ribosomal RNA Genes. PLoS One 4: e6372. project name LEVECO protocol label sequencial filtration and preservation salinity 38.9 sample collection device or method sequencial filtration using peristaltic pump sample name LV_26 sample storage duration 3 sample storage temperature -80 sampling campaign cruise sampling platform Research Vessel HCMR sampling site LV_st_10 scientific_name marine metagenome target gene 18S temperature 17 total depth of water column 3400 ERS2429895 SAMEA4609201 408172 marine metagenome Levantine Sea (Eastern Mediterranean); plankton; eukaryotic and prokaryotic communities composition; amplicons; illumina MiSeq; size fractionation by filtration DNA extraction method PowerWater DNA isolation kit ENA-FIRST-PUBLIC 2019-06-23T04:04:02Z ENA-LAST-UPDATE 2018-04-25T07:47:42Z External Id SAMEA4609201 INSDC center name HELLENIC CENTRE FOR MARINE RESEARCH INSDC first public 2019-06-23T04:04:02Z INSDC last update 2018-04-25T07:47:42Z INSDC status public Latitude End 34.6667 Longitude End 24.3667 Submitter Id LV_28 amount or size of sample collected 30 depth 1000 environment (biome) temperate mediterannean sea biome environment (feature) marine pelagic feature environment (material) planktonic material environmental package water event date/time 2016-04-15T21:15:00Z fraction_microns 20 latitude start 34.6667 longitude start 24.3667 marine region Levantine basin (eastern Mediterannean) primer_f 5'CCCTGCCHTTTGTACACAC primer_f_reference Amaral-Zettler LA, McCliment EA, Ducklow HW, Huse SM. (2009). A Method for Studying Protistan Diversity Using Massively Parallel Sequencing of V9 Hypervariable Regions of Small-Subunit Ribosomal RNA Genes. PLoS One 4: e6372. primer_r 5'CCTTCYGCAGGTTCACCTAC primer_r_reference Amaral-Zettler LA, McCliment EA, Ducklow HW, Huse SM. (2009). A Method for Studying Protistan Diversity Using Massively Parallel Sequencing of V9 Hypervariable Regions of Small-Subunit Ribosomal RNA Genes. PLoS One 4: e6372. project name LEVECO protocol label sequencial filtration and preservation salinity 38.765 sample collection device or method sequencial filtration using peristaltic pump sample name LV_28 sample storage duration 3 sample storage temperature -80 sampling campaign cruise sampling platform Research Vessel HCMR sampling site LV_st_10 scientific_name marine metagenome target gene 18S temperature 13.68 total depth of water column 3400 ERS2429896 SAMEA4609202 408172 marine metagenome Levantine Sea (Eastern Mediterranean); plankton; eukaryotic and prokaryotic communities composition; amplicons; illumina MiSeq; size fractionation by filtration DNA extraction method PowerWater DNA isolation kit ENA-FIRST-PUBLIC 2019-06-23T04:04:02Z ENA-LAST-UPDATE 2018-04-25T07:47:42Z External Id SAMEA4609202 INSDC center name HELLENIC CENTRE FOR MARINE RESEARCH INSDC first public 2019-06-23T04:04:02Z INSDC last update 2018-04-25T07:47:42Z INSDC status public Latitude End 34.6667 Longitude End 24.3667 Submitter Id LV_29 amount or size of sample collected 30 depth 1000 environment (biome) temperate mediterannean sea biome environment (feature) marine pelagic feature environment (material) planktonic material environmental package water event date/time 2016-04-15T21:15:00Z fraction_microns 0.8 latitude start 34.6667 longitude start 24.3667 marine region Levantine basin (eastern Mediterannean) primer_f 5'CCCTGCCHTTTGTACACAC primer_f_reference Amaral-Zettler LA, McCliment EA, Ducklow HW, Huse SM. (2009). A Method for Studying Protistan Diversity Using Massively Parallel Sequencing of V9 Hypervariable Regions of Small-Subunit Ribosomal RNA Genes. PLoS One 4: e6372. primer_r 5'CCTTCYGCAGGTTCACCTAC primer_r_reference Amaral-Zettler LA, McCliment EA, Ducklow HW, Huse SM. (2009). A Method for Studying Protistan Diversity Using Massively Parallel Sequencing of V9 Hypervariable Regions of Small-Subunit Ribosomal RNA Genes. PLoS One 4: e6372. project name LEVECO protocol label sequencial filtration and preservation salinity 38.765 sample collection device or method sequencial filtration using peristaltic pump sample name LV_29 sample storage duration 3 sample storage temperature -80 sampling campaign cruise sampling platform Research Vessel HCMR sampling site LV_st_10 scientific_name marine metagenome target gene 18S temperature 13.68 total depth of water column 3400 ERS2429898 SAMEA4609204 408172 marine metagenome Levantine Sea (Eastern Mediterranean); plankton; eukaryotic and prokaryotic communities composition; amplicons; illumina MiSeq; size fractionation by filtration DNA extraction method PowerWater DNA isolation kit ENA-FIRST-PUBLIC 2019-06-23T04:04:02Z ENA-LAST-UPDATE 2018-04-25T07:47:42Z External Id SAMEA4609204 INSDC center name HELLENIC CENTRE FOR MARINE RESEARCH INSDC first public 2019-06-23T04:04:02Z INSDC last update 2018-04-25T07:47:42Z INSDC status public Latitude End 34.25 Longitude End 25.4833 Submitter Id LV_31 amount or size of sample collected 30 depth 1000 environment (biome) temperate mediterannean sea biome environment (feature) marine pelagic feature environment (material) planktonic material environmental package water event date/time 2016-04-14T09:30:00Z fraction_microns 20 latitude start 34.25 longitude start 25.4833 marine region Levantine basin (eastern Mediterannean) primer_f 5'CCCTGCCHTTTGTACACAC primer_f_reference Amaral-Zettler LA, McCliment EA, Ducklow HW, Huse SM. (2009). A Method for Studying Protistan Diversity Using Massively Parallel Sequencing of V9 Hypervariable Regions of Small-Subunit Ribosomal RNA Genes. PLoS One 4: e6372. primer_r 5'CCTTCYGCAGGTTCACCTAC primer_r_reference Amaral-Zettler LA, McCliment EA, Ducklow HW, Huse SM. (2009). A Method for Studying Protistan Diversity Using Massively Parallel Sequencing of V9 Hypervariable Regions of Small-Subunit Ribosomal RNA Genes. PLoS One 4: e6372. project name LEVECO protocol label sequencial filtration and preservation salinity 38.78 sample collection device or method sequencial filtration using peristaltic pump sample name LV_31 sample storage duration 3 sample storage temperature -80 sampling campaign cruise sampling platform Research Vessel HCMR sampling site LV_st_13 scientific_name marine metagenome target gene 18S temperature 13.7 total depth of water column 3300 ERS2429901 SAMEA4609207 408172 marine metagenome Levantine Sea (Eastern Mediterranean); plankton; eukaryotic and prokaryotic communities composition; amplicons; illumina MiSeq; size fractionation by filtration DNA extraction method PowerWater DNA isolation kit ENA-FIRST-PUBLIC 2019-06-23T04:04:02Z ENA-LAST-UPDATE 2018-04-25T07:47:43Z External Id SAMEA4609207 INSDC center name HELLENIC CENTRE FOR MARINE RESEARCH INSDC first public 2019-06-23T04:04:02Z INSDC last update 2018-04-25T07:47:43Z INSDC status public Latitude End 34.25 Longitude End 25.4833 Submitter Id LV_34 amount or size of sample collected 20 depth 5 environment (biome) temperate mediterannean sea biome environment (feature) marine pelagic feature environment (material) planktonic material environmental package water event date/time 2016-04-14T10:30:00Z fraction_microns 20 latitude start 34.25 longitude start 25.4833 marine region Levantine basin (eastern Mediterannean) primer_f 5'CCCTGCCHTTTGTACACAC primer_f_reference Amaral-Zettler LA, McCliment EA, Ducklow HW, Huse SM. (2009). A Method for Studying Protistan Diversity Using Massively Parallel Sequencing of V9 Hypervariable Regions of Small-Subunit Ribosomal RNA Genes. PLoS One 4: e6372. primer_r 5'CCTTCYGCAGGTTCACCTAC primer_r_reference Amaral-Zettler LA, McCliment EA, Ducklow HW, Huse SM. (2009). A Method for Studying Protistan Diversity Using Massively Parallel Sequencing of V9 Hypervariable Regions of Small-Subunit Ribosomal RNA Genes. PLoS One 4: e6372. project name LEVECO protocol label sequencial filtration and preservation salinity 39.05 sample collection device or method sequencial filtration using peristaltic pump sample name LV_34 sample storage duration 3 sample storage temperature -80 sampling campaign cruise sampling platform Research Vessel HCMR sampling site LV_st_13 scientific_name marine metagenome target gene 18S temperature 17.5 total depth of water column 3300 ERS2429902 SAMEA4609208 408172 marine metagenome Levantine Sea (Eastern Mediterranean); plankton; eukaryotic and prokaryotic communities composition; amplicons; illumina MiSeq; size fractionation by filtration DNA extraction method PowerWater DNA isolation kit ENA-FIRST-PUBLIC 2019-06-23T04:04:02Z ENA-LAST-UPDATE 2018-04-25T07:47:43Z External Id SAMEA4609208 INSDC center name HELLENIC CENTRE FOR MARINE RESEARCH INSDC first public 2019-06-23T04:04:02Z INSDC last update 2018-04-25T07:47:43Z INSDC status public Latitude End 34.25 Longitude End 25.4833 Submitter Id LV_35 amount or size of sample collected 20 depth 5 environment (biome) temperate mediterannean sea biome environment (feature) marine pelagic feature environment (material) planktonic material environmental package water event date/time 2016-04-14T10:30:00Z fraction_microns 0.8 latitude start 34.25 longitude start 25.4833 marine region Levantine basin (eastern Mediterannean) primer_f 5'CCCTGCCHTTTGTACACAC primer_f_reference Amaral-Zettler LA, McCliment EA, Ducklow HW, Huse SM. (2009). A Method for Studying Protistan Diversity Using Massively Parallel Sequencing of V9 Hypervariable Regions of Small-Subunit Ribosomal RNA Genes. PLoS One 4: e6372. primer_r 5'CCTTCYGCAGGTTCACCTAC primer_r_reference Amaral-Zettler LA, McCliment EA, Ducklow HW, Huse SM. (2009). A Method for Studying Protistan Diversity Using Massively Parallel Sequencing of V9 Hypervariable Regions of Small-Subunit Ribosomal RNA Genes. PLoS One 4: e6372. project name LEVECO protocol label sequencial filtration and preservation salinity 39.05 sample collection device or method sequencial filtration using peristaltic pump sample name LV_35 sample storage duration 3 sample storage temperature -80 sampling campaign cruise sampling platform Research Vessel HCMR sampling site LV_st_13 scientific_name marine metagenome target gene 18S temperature 17.5 total depth of water column 3300 ERS2429904 SAMEA4609210 408172 marine metagenome Levantine Sea (Eastern Mediterranean); plankton; eukaryotic and prokaryotic communities composition; amplicons; illumina MiSeq; size fractionation by filtration DNA extraction method PowerWater DNA isolation kit ENA-FIRST-PUBLIC 2019-06-23T04:04:02Z ENA-LAST-UPDATE 2018-04-25T07:47:43Z External Id SAMEA4609210 INSDC center name HELLENIC CENTRE FOR MARINE RESEARCH INSDC first public 2019-06-23T04:04:02Z INSDC last update 2018-04-25T07:47:43Z INSDC status public Latitude End 34.25 Longitude End 25.4833 Submitter Id LV_37 amount or size of sample collected 20 depth 75 environment (biome) temperate mediterannean sea biome environment (feature) marine pelagic feature environment (material) planktonic material environmental package water event date/time 2016-04-14T10:30:00Z fraction_microns 20 latitude start 34.25 longitude start 25.4833 marine region Levantine basin (eastern Mediterannean) primer_f 5'CCCTGCCHTTTGTACACAC primer_f_reference Amaral-Zettler LA, McCliment EA, Ducklow HW, Huse SM. (2009). A Method for Studying Protistan Diversity Using Massively Parallel Sequencing of V9 Hypervariable Regions of Small-Subunit Ribosomal RNA Genes. PLoS One 4: e6372. primer_r 5'CCTTCYGCAGGTTCACCTAC primer_r_reference Amaral-Zettler LA, McCliment EA, Ducklow HW, Huse SM. (2009). A Method for Studying Protistan Diversity Using Massively Parallel Sequencing of V9 Hypervariable Regions of Small-Subunit Ribosomal RNA Genes. PLoS One 4: e6372. project name LEVECO protocol label sequencial filtration and preservation salinity 39.05 sample collection device or method sequencial filtration using peristaltic pump sample name LV_37 sample storage duration 3 sample storage temperature -80 sampling campaign cruise sampling platform Research Vessel HCMR sampling site LV_st_13 scientific_name marine metagenome target gene 18S temperature 17 total depth of water column 3300 ERS2429905 SAMEA4609211 408172 marine metagenome Levantine Sea (Eastern Mediterranean); plankton; eukaryotic and prokaryotic communities composition; amplicons; illumina MiSeq; size fractionation by filtration DNA extraction method PowerWater DNA isolation kit ENA-FIRST-PUBLIC 2019-06-23T04:04:02Z ENA-LAST-UPDATE 2018-04-25T07:47:43Z External Id SAMEA4609211 INSDC center name HELLENIC CENTRE FOR MARINE RESEARCH INSDC first public 2019-06-23T04:04:02Z INSDC last update 2018-04-25T07:47:43Z INSDC status public Latitude End 34.25 Longitude End 25.4833 Submitter Id LV_38 amount or size of sample collected 20 depth 75 environment (biome) temperate mediterannean sea biome environment (feature) marine pelagic feature environment (material) planktonic material environmental package water event date/time 2016-04-14T10:30:00Z fraction_microns 0.8 latitude start 34.25 longitude start 25.4833 marine region Levantine basin (eastern Mediterannean) primer_f 5'CCCTGCCHTTTGTACACAC primer_f_reference Amaral-Zettler LA, McCliment EA, Ducklow HW, Huse SM. (2009). A Method for Studying Protistan Diversity Using Massively Parallel Sequencing of V9 Hypervariable Regions of Small-Subunit Ribosomal RNA Genes. PLoS One 4: e6372. primer_r 5'CCTTCYGCAGGTTCACCTAC primer_r_reference Amaral-Zettler LA, McCliment EA, Ducklow HW, Huse SM. (2009). A Method for Studying Protistan Diversity Using Massively Parallel Sequencing of V9 Hypervariable Regions of Small-Subunit Ribosomal RNA Genes. PLoS One 4: e6372. project name LEVECO protocol label sequencial filtration and preservation salinity 39.05 sample collection device or method sequencial filtration using peristaltic pump sample name LV_38 sample storage duration 3 sample storage temperature -80 sampling campaign cruise sampling platform Research Vessel HCMR sampling site LV_st_13 scientific_name marine metagenome target gene 18S temperature 17 total depth of water column 3300 ERS2429907 SAMEA4609213 408172 marine metagenome Levantine Sea (Eastern Mediterranean); plankton; eukaryotic and prokaryotic communities composition; amplicons; illumina MiSeq; size fractionation by filtration DNA extraction method PowerWater DNA isolation kit ENA-FIRST-PUBLIC 2019-06-23T04:04:02Z ENA-LAST-UPDATE 2018-04-25T07:47:43Z External Id SAMEA4609213 INSDC center name HELLENIC CENTRE FOR MARINE RESEARCH INSDC first public 2019-06-23T04:04:02Z INSDC last update 2018-04-25T07:47:43Z INSDC status public Latitude End 34.25 Longitude End 25.4833 Submitter Id LV_40 amount or size of sample collected 30 depth 3300 environment (biome) temperate mediterannean sea biome environment (feature) marine pelagic feature environment (material) planktonic material environmental package water event date/time 2016-04-14T17:00:00Z fraction_microns 20 latitude start 34.25 longitude start 25.4833 marine region Levantine basin (eastern Mediterannean) primer_f 5'CCCTGCCHTTTGTACACAC primer_f_reference Amaral-Zettler LA, McCliment EA, Ducklow HW, Huse SM. (2009). A Method for Studying Protistan Diversity Using Massively Parallel Sequencing of V9 Hypervariable Regions of Small-Subunit Ribosomal RNA Genes. PLoS One 4: e6372. primer_r 5'CCTTCYGCAGGTTCACCTAC primer_r_reference Amaral-Zettler LA, McCliment EA, Ducklow HW, Huse SM. (2009). A Method for Studying Protistan Diversity Using Massively Parallel Sequencing of V9 Hypervariable Regions of Small-Subunit Ribosomal RNA Genes. PLoS One 4: e6372. project name LEVECO protocol label sequencial filtration and preservation salinity 38.745 sample collection device or method sequencial filtration using peristaltic pump sample name LV_40 sample storage duration 3 sample storage temperature -80 sampling campaign cruise sampling platform Research Vessel HCMR sampling site LV_st_13 scientific_name marine metagenome target gene 18S temperature 13.5 total depth of water column 3300 ERS2429908 SAMEA4609214 408172 marine metagenome Levantine Sea (Eastern Mediterranean); plankton; eukaryotic and prokaryotic communities composition; amplicons; illumina MiSeq; size fractionation by filtration DNA extraction method PowerWater DNA isolation kit ENA-FIRST-PUBLIC 2019-06-23T04:04:02Z ENA-LAST-UPDATE 2018-04-25T07:47:43Z External Id SAMEA4609214 INSDC center name HELLENIC CENTRE FOR MARINE RESEARCH INSDC first public 2019-06-23T04:04:02Z INSDC last update 2018-04-25T07:47:43Z INSDC status public Latitude End 34.25 Longitude End 25.4833 Submitter Id LV_41 amount or size of sample collected 30 depth 3300 environment (biome) temperate mediterannean sea biome environment (feature) marine pelagic feature environment (material) planktonic material environmental package water event date/time 2016-04-14T17:00:00Z fraction_microns 0.8 latitude start 34.25 longitude start 25.4833 marine region Levantine basin (eastern Mediterannean) primer_f 5'CCCTGCCHTTTGTACACAC primer_f_reference Amaral-Zettler LA, McCliment EA, Ducklow HW, Huse SM. (2009). A Method for Studying Protistan Diversity Using Massively Parallel Sequencing of V9 Hypervariable Regions of Small-Subunit Ribosomal RNA Genes. PLoS One 4: e6372. primer_r 5'CCTTCYGCAGGTTCACCTAC primer_r_reference Amaral-Zettler LA, McCliment EA, Ducklow HW, Huse SM. (2009). A Method for Studying Protistan Diversity Using Massively Parallel Sequencing of V9 Hypervariable Regions of Small-Subunit Ribosomal RNA Genes. PLoS One 4: e6372. project name LEVECO protocol label sequencial filtration and preservation salinity 38.745 sample collection device or method sequencial filtration using peristaltic pump sample name LV_41 sample storage duration 3 sample storage temperature -80 sampling campaign cruise sampling platform Research Vessel HCMR sampling site LV_st_13 scientific_name marine metagenome target gene 18S temperature 13.5 total depth of water column 3300 ERS2429910 SAMEA4609216 408172 marine metagenome Levantine Sea (Eastern Mediterranean); plankton; eukaryotic and prokaryotic communities composition; amplicons; illumina MiSeq; size fractionation by filtration DNA extraction method PowerWater DNA isolation kit ENA-FIRST-PUBLIC 2019-06-23T04:04:02Z ENA-LAST-UPDATE 2018-04-25T07:47:43Z External Id SAMEA4609216 INSDC center name HELLENIC CENTRE FOR MARINE RESEARCH INSDC first public 2019-06-23T04:04:02Z INSDC last update 2018-04-25T07:47:43Z INSDC status public Latitude End 34.25 Longitude End 25.4833 Submitter Id LV_43 amount or size of sample collected 20 depth 50 environment (biome) temperate mediterannean sea biome environment (feature) marine pelagic feature environment (material) planktonic material environmental package water event date/time 2016-04-14T10:30:00Z fraction_microns 20 latitude start 34.25 longitude start 25.4833 marine region Levantine basin (eastern Mediterannean) primer_f 5'CCCTGCCHTTTGTACACAC primer_f_reference Amaral-Zettler LA, McCliment EA, Ducklow HW, Huse SM. (2009). A Method for Studying Protistan Diversity Using Massively Parallel Sequencing of V9 Hypervariable Regions of Small-Subunit Ribosomal RNA Genes. PLoS One 4: e6372. primer_r 5'CCTTCYGCAGGTTCACCTAC primer_r_reference Amaral-Zettler LA, McCliment EA, Ducklow HW, Huse SM. (2009). A Method for Studying Protistan Diversity Using Massively Parallel Sequencing of V9 Hypervariable Regions of Small-Subunit Ribosomal RNA Genes. PLoS One 4: e6372. project name LEVECO protocol label sequencial filtration and preservation salinity 39.05 sample collection device or method sequencial filtration using peristaltic pump sample name LV_43 sample storage duration 3 sample storage temperature -80 sampling campaign cruise sampling platform Research Vessel HCMR sampling site LV_st_13 scientific_name marine metagenome target gene 18S temperature 17.5 total depth of water column 3300 ERS2429911 SAMEA4609217 408172 marine metagenome Levantine Sea (Eastern Mediterranean); plankton; eukaryotic and prokaryotic communities composition; amplicons; illumina MiSeq; size fractionation by filtration DNA extraction method PowerWater DNA isolation kit ENA-FIRST-PUBLIC 2019-06-23T04:04:02Z ENA-LAST-UPDATE 2018-04-25T07:47:43Z External Id SAMEA4609217 INSDC center name HELLENIC CENTRE FOR MARINE RESEARCH INSDC first public 2019-06-23T04:04:02Z INSDC last update 2018-04-25T07:47:43Z INSDC status public Latitude End 34.25 Longitude End 25.4833 Submitter Id LV_44 amount or size of sample collected 20 depth 50 environment (biome) temperate mediterannean sea biome environment (feature) marine pelagic feature environment (material) planktonic material environmental package water event date/time 2016-04-14T10:30:00Z fraction_microns 0.8 latitude start 34.25 longitude start 25.4833 marine region Levantine basin (eastern Mediterannean) primer_f 5'CCCTGCCHTTTGTACACAC primer_f_reference Amaral-Zettler LA, McCliment EA, Ducklow HW, Huse SM. (2009). A Method for Studying Protistan Diversity Using Massively Parallel Sequencing of V9 Hypervariable Regions of Small-Subunit Ribosomal RNA Genes. PLoS One 4: e6372. primer_r 5'CCTTCYGCAGGTTCACCTAC primer_r_reference Amaral-Zettler LA, McCliment EA, Ducklow HW, Huse SM. (2009). A Method for Studying Protistan Diversity Using Massively Parallel Sequencing of V9 Hypervariable Regions of Small-Subunit Ribosomal RNA Genes. PLoS One 4: e6372. project name LEVECO protocol label sequencial filtration and preservation salinity 39.05 sample collection device or method sequencial filtration using peristaltic pump sample name LV_44 sample storage duration 3 sample storage temperature -80 sampling campaign cruise sampling platform Research Vessel HCMR sampling site LV_st_13 scientific_name marine metagenome target gene 18S temperature 17.5 total depth of water column 3300 ERS2429913 SAMEA4609219 408172 marine metagenome Levantine Sea (Eastern Mediterranean); plankton; eukaryotic and prokaryotic communities composition; amplicons; illumina MiSeq; size fractionation by filtration DNA extraction method PowerWater DNA isolation kit ENA-FIRST-PUBLIC 2019-06-23T04:04:02Z ENA-LAST-UPDATE 2018-04-25T07:47:43Z External Id SAMEA4609219 INSDC center name HELLENIC CENTRE FOR MARINE RESEARCH INSDC first public 2019-06-23T04:04:02Z INSDC last update 2018-04-25T07:47:43Z INSDC status public Latitude End 34.4333 Longitude End 26.3833 Submitter Id LV_46 amount or size of sample collected 45 depth 1000 environment (biome) temperate mediterannean sea biome environment (feature) marine pelagic feature environment (material) planktonic material environmental package water event date/time 2016-04-10T09:00:00Z fraction_microns 20 latitude start 34.4333 longitude start 26.3833 marine region Levantine basin (eastern Mediterannean) primer_f 5'CCCTGCCHTTTGTACACAC primer_f_reference Amaral-Zettler LA, McCliment EA, Ducklow HW, Huse SM. (2009). A Method for Studying Protistan Diversity Using Massively Parallel Sequencing of V9 Hypervariable Regions of Small-Subunit Ribosomal RNA Genes. PLoS One 4: e6372. primer_r 5'CCTTCYGCAGGTTCACCTAC primer_r_reference Amaral-Zettler LA, McCliment EA, Ducklow HW, Huse SM. (2009). A Method for Studying Protistan Diversity Using Massively Parallel Sequencing of V9 Hypervariable Regions of Small-Subunit Ribosomal RNA Genes. PLoS One 4: e6372. project name LEVECO protocol label sequencial filtration and preservation salinity 38.765 sample collection device or method sequencial filtration using peristaltic pump sample name LV_46 sample storage duration 3 sample storage temperature -80 sampling campaign cruise sampling platform Research Vessel HCMR sampling site LV_st_18 scientific_name marine metagenome target gene 18S temperature 13.7 total depth of water column 3500 ERS2429914 SAMEA4609220 408172 marine metagenome Levantine Sea (Eastern Mediterranean); plankton; eukaryotic and prokaryotic communities composition; amplicons; illumina MiSeq; size fractionation by filtration DNA extraction method PowerWater DNA isolation kit ENA-FIRST-PUBLIC 2019-06-23T04:04:02Z ENA-LAST-UPDATE 2018-04-25T07:47:43Z External Id SAMEA4609220 INSDC center name HELLENIC CENTRE FOR MARINE RESEARCH INSDC first public 2019-06-23T04:04:02Z INSDC last update 2018-04-25T07:47:43Z INSDC status public Latitude End 34.4333 Longitude End 26.3833 Submitter Id LV_47 amount or size of sample collected 45 depth 1000 environment (biome) temperate mediterannean sea biome environment (feature) marine pelagic feature environment (material) planktonic material environmental package water event date/time 2016-04-10T09:00:00Z fraction_microns 0.8 latitude start 34.4333 longitude start 26.3833 marine region Levantine basin (eastern Mediterannean) primer_f 5'CCCTGCCHTTTGTACACAC primer_f_reference Amaral-Zettler LA, McCliment EA, Ducklow HW, Huse SM. (2009). A Method for Studying Protistan Diversity Using Massively Parallel Sequencing of V9 Hypervariable Regions of Small-Subunit Ribosomal RNA Genes. PLoS One 4: e6372. primer_r 5'CCTTCYGCAGGTTCACCTAC primer_r_reference Amaral-Zettler LA, McCliment EA, Ducklow HW, Huse SM. (2009). A Method for Studying Protistan Diversity Using Massively Parallel Sequencing of V9 Hypervariable Regions of Small-Subunit Ribosomal RNA Genes. PLoS One 4: e6372. project name LEVECO protocol label sequencial filtration and preservation salinity 38.765 sample collection device or method sequencial filtration using peristaltic pump sample name LV_47 sample storage duration 3 sample storage temperature -80 sampling campaign cruise sampling platform Research Vessel HCMR sampling site LV_st_18 scientific_name marine metagenome target gene 18S temperature 13.7 total depth of water column 3500 ERS2429916 SAMEA4609222 408172 marine metagenome Levantine Sea (Eastern Mediterranean); plankton; eukaryotic and prokaryotic communities composition; amplicons; illumina MiSeq; size fractionation by filtration DNA extraction method PowerWater DNA isolation kit ENA-FIRST-PUBLIC 2019-06-23T04:04:02Z ENA-LAST-UPDATE 2018-04-25T07:47:43Z External Id SAMEA4609222 INSDC center name HELLENIC CENTRE FOR MARINE RESEARCH INSDC first public 2019-06-23T04:04:02Z INSDC last update 2018-04-25T07:47:43Z INSDC status public Latitude End 34.4333 Longitude End 26.3833 Submitter Id LV_49 amount or size of sample collected 18 depth 5 environment (biome) temperate mediterannean sea biome environment (feature) marine pelagic feature environment (material) planktonic material environmental package water event date/time 2016-04-10T11:00:00Z fraction_microns 20 latitude start 34.4333 longitude start 26.3833 marine region Levantine basin (eastern Mediterannean) primer_f 5'CCCTGCCHTTTGTACACAC primer_f_reference Amaral-Zettler LA, McCliment EA, Ducklow HW, Huse SM. (2009). A Method for Studying Protistan Diversity Using Massively Parallel Sequencing of V9 Hypervariable Regions of Small-Subunit Ribosomal RNA Genes. PLoS One 4: e6372. primer_r 5'CCTTCYGCAGGTTCACCTAC primer_r_reference Amaral-Zettler LA, McCliment EA, Ducklow HW, Huse SM. (2009). A Method for Studying Protistan Diversity Using Massively Parallel Sequencing of V9 Hypervariable Regions of Small-Subunit Ribosomal RNA Genes. PLoS One 4: e6372. project name LEVECO protocol label sequencial filtration and preservation salinity 39.05 sample collection device or method sequencial filtration using peristaltic pump sample name LV_49 sample storage duration 3 sample storage temperature -80 sampling campaign cruise sampling platform Research Vessel HCMR sampling site LV_st_18 scientific_name marine metagenome target gene 18S temperature 17.5 total depth of water column 3500 ERS2429917 SAMEA4609223 408172 marine metagenome Levantine Sea (Eastern Mediterranean); plankton; eukaryotic and prokaryotic communities composition; amplicons; illumina MiSeq; size fractionation by filtration DNA extraction method PowerWater DNA isolation kit ENA-FIRST-PUBLIC 2019-06-23T04:04:02Z ENA-LAST-UPDATE 2018-04-25T07:47:43Z External Id SAMEA4609223 INSDC center name HELLENIC CENTRE FOR MARINE RESEARCH INSDC first public 2019-06-23T04:04:02Z INSDC last update 2018-04-25T07:47:43Z INSDC status public Latitude End 34.4333 Longitude End 26.3833 Submitter Id LV_50 amount or size of sample collected 18 depth 5 environment (biome) temperate mediterannean sea biome environment (feature) marine pelagic feature environment (material) planktonic material environmental package water event date/time 2016-04-10T11:00:00Z fraction_microns 0.8 latitude start 34.4333 longitude start 26.3833 marine region Levantine basin (eastern Mediterannean) primer_f 5'CCCTGCCHTTTGTACACAC primer_f_reference Amaral-Zettler LA, McCliment EA, Ducklow HW, Huse SM. (2009). A Method for Studying Protistan Diversity Using Massively Parallel Sequencing of V9 Hypervariable Regions of Small-Subunit Ribosomal RNA Genes. PLoS One 4: e6372. primer_r 5'CCTTCYGCAGGTTCACCTAC primer_r_reference Amaral-Zettler LA, McCliment EA, Ducklow HW, Huse SM. (2009). A Method for Studying Protistan Diversity Using Massively Parallel Sequencing of V9 Hypervariable Regions of Small-Subunit Ribosomal RNA Genes. PLoS One 4: e6372. project name LEVECO protocol label sequencial filtration and preservation salinity 39.05 sample collection device or method sequencial filtration using peristaltic pump sample name LV_50 sample storage duration 3 sample storage temperature -80 sampling campaign cruise sampling platform Research Vessel HCMR sampling site LV_st_18 scientific_name marine metagenome target gene 18S temperature 17.5 total depth of water column 3500 ERS2429919 SAMEA4609225 408172 marine metagenome Levantine Sea (Eastern Mediterranean); plankton; eukaryotic and prokaryotic communities composition; amplicons; illumina MiSeq; size fractionation by filtration DNA extraction method PowerWater DNA isolation kit ENA-FIRST-PUBLIC 2019-06-23T04:04:02Z ENA-LAST-UPDATE 2018-04-25T07:47:43Z External Id SAMEA4609225 INSDC center name HELLENIC CENTRE FOR MARINE RESEARCH INSDC first public 2019-06-23T04:04:02Z INSDC last update 2018-04-25T07:47:43Z INSDC status public Latitude End 34.4333 Longitude End 26.3833 Submitter Id LV_52 amount or size of sample collected 18 depth 75 environment (biome) temperate mediterannean sea biome environment (feature) marine pelagic feature environment (material) planktonic material environmental package water event date/time 2016-04-10T11:00:00Z fraction_microns 20 latitude start 34.4333 longitude start 26.3833 marine region Levantine basin (eastern Mediterannean) primer_f 5'CCCTGCCHTTTGTACACAC primer_f_reference Amaral-Zettler LA, McCliment EA, Ducklow HW, Huse SM. (2009). A Method for Studying Protistan Diversity Using Massively Parallel Sequencing of V9 Hypervariable Regions of Small-Subunit Ribosomal RNA Genes. PLoS One 4: e6372. primer_r 5'CCTTCYGCAGGTTCACCTAC primer_r_reference Amaral-Zettler LA, McCliment EA, Ducklow HW, Huse SM. (2009). A Method for Studying Protistan Diversity Using Massively Parallel Sequencing of V9 Hypervariable Regions of Small-Subunit Ribosomal RNA Genes. PLoS One 4: e6372. project name LEVECO protocol label sequencial filtration and preservation salinity 39.05 sample collection device or method sequencial filtration using peristaltic pump sample name LV_52 sample storage duration 3 sample storage temperature -80 sampling campaign cruise sampling platform Research Vessel HCMR sampling site LV_st_18 scientific_name marine metagenome target gene 18S temperature 16.5 total depth of water column 3500 ERS2429920 SAMEA4609226 408172 marine metagenome Levantine Sea (Eastern Mediterranean); plankton; eukaryotic and prokaryotic communities composition; amplicons; illumina MiSeq; size fractionation by filtration DNA extraction method PowerWater DNA isolation kit ENA-FIRST-PUBLIC 2019-06-23T04:04:02Z ENA-LAST-UPDATE 2018-04-25T07:47:43Z External Id SAMEA4609226 INSDC center name HELLENIC CENTRE FOR MARINE RESEARCH INSDC first public 2019-06-23T04:04:02Z INSDC last update 2018-04-25T07:47:43Z INSDC status public Latitude End 34.4333 Longitude End 26.3833 Submitter Id LV_53 amount or size of sample collected 18 depth 75 environment (biome) temperate mediterannean sea biome environment (feature) marine pelagic feature environment (material) planktonic material environmental package water event date/time 2016-04-10T11:00:00Z fraction_microns 0.8 latitude start 34.4333 longitude start 26.3833 marine region Levantine basin (eastern Mediterannean) primer_f 5'CCCTGCCHTTTGTACACAC primer_f_reference Amaral-Zettler LA, McCliment EA, Ducklow HW, Huse SM. (2009). A Method for Studying Protistan Diversity Using Massively Parallel Sequencing of V9 Hypervariable Regions of Small-Subunit Ribosomal RNA Genes. PLoS One 4: e6372. primer_r 5'CCTTCYGCAGGTTCACCTAC primer_r_reference Amaral-Zettler LA, McCliment EA, Ducklow HW, Huse SM. (2009). A Method for Studying Protistan Diversity Using Massively Parallel Sequencing of V9 Hypervariable Regions of Small-Subunit Ribosomal RNA Genes. PLoS One 4: e6372. project name LEVECO protocol label sequencial filtration and preservation salinity 39.05 sample collection device or method sequencial filtration using peristaltic pump sample name LV_53 sample storage duration 3 sample storage temperature -80 sampling campaign cruise sampling platform Research Vessel HCMR sampling site LV_st_18 scientific_name marine metagenome target gene 18S temperature 16.5 total depth of water column 3500 ERS2429922 SAMEA4609228 408172 marine metagenome Levantine Sea (Eastern Mediterranean); plankton; eukaryotic and prokaryotic communities composition; amplicons; illumina MiSeq; size fractionation by filtration DNA extraction method PowerWater DNA isolation kit ENA-FIRST-PUBLIC 2019-06-23T04:04:02Z ENA-LAST-UPDATE 2018-04-25T07:47:43Z External Id SAMEA4609228 INSDC center name HELLENIC CENTRE FOR MARINE RESEARCH INSDC first public 2019-06-23T04:04:02Z INSDC last update 2018-04-25T07:47:43Z INSDC status public Latitude End 34.4333 Longitude End 26.3833 Submitter Id LV_55 amount or size of sample collected 20 depth 50 environment (biome) temperate mediterannean sea biome environment (feature) marine pelagic feature environment (material) planktonic material environmental package water event date/time 2016-04-10T11:00:00Z fraction_microns 20 latitude start 34.4333 longitude start 26.3833 marine region Levantine basin (eastern Mediterannean) primer_f 5'CCCTGCCHTTTGTACACAC primer_f_reference Amaral-Zettler LA, McCliment EA, Ducklow HW, Huse SM. (2009). A Method for Studying Protistan Diversity Using Massively Parallel Sequencing of V9 Hypervariable Regions of Small-Subunit Ribosomal RNA Genes. PLoS One 4: e6372. primer_r 5'CCTTCYGCAGGTTCACCTAC primer_r_reference Amaral-Zettler LA, McCliment EA, Ducklow HW, Huse SM. (2009). A Method for Studying Protistan Diversity Using Massively Parallel Sequencing of V9 Hypervariable Regions of Small-Subunit Ribosomal RNA Genes. PLoS One 4: e6372. project name LEVECO protocol label sequencial filtration and preservation salinity 39.05 sample collection device or method sequencial filtration using peristaltic pump sample name LV_55 sample storage duration 3 sample storage temperature -80 sampling campaign cruise sampling platform Research Vessel HCMR sampling site LV_st_18 scientific_name marine metagenome target gene 18S temperature 17 total depth of water column 3500 ERS2429923 SAMEA4609229 408172 marine metagenome Levantine Sea (Eastern Mediterranean); plankton; eukaryotic and prokaryotic communities composition; amplicons; illumina MiSeq; size fractionation by filtration DNA extraction method PowerWater DNA isolation kit ENA-FIRST-PUBLIC 2019-06-23T04:04:02Z ENA-LAST-UPDATE 2018-04-25T07:47:43Z External Id SAMEA4609229 INSDC center name HELLENIC CENTRE FOR MARINE RESEARCH INSDC first public 2019-06-23T04:04:02Z INSDC last update 2018-04-25T07:47:43Z INSDC status public Latitude End 34.4333 Longitude End 26.3833 Submitter Id LV_56 amount or size of sample collected 20 depth 50 environment (biome) temperate mediterannean sea biome environment (feature) marine pelagic feature environment (material) planktonic material environmental package water event date/time 2016-04-10T11:00:00Z fraction_microns 0.8 latitude start 34.4333 longitude start 26.3833 marine region Levantine basin (eastern Mediterannean) primer_f 5'CCCTGCCHTTTGTACACAC primer_f_reference Amaral-Zettler LA, McCliment EA, Ducklow HW, Huse SM. (2009). A Method for Studying Protistan Diversity Using Massively Parallel Sequencing of V9 Hypervariable Regions of Small-Subunit Ribosomal RNA Genes. PLoS One 4: e6372. primer_r 5'CCTTCYGCAGGTTCACCTAC primer_r_reference Amaral-Zettler LA, McCliment EA, Ducklow HW, Huse SM. (2009). A Method for Studying Protistan Diversity Using Massively Parallel Sequencing of V9 Hypervariable Regions of Small-Subunit Ribosomal RNA Genes. PLoS One 4: e6372. project name LEVECO protocol label sequencial filtration and preservation salinity 39.05 sample collection device or method sequencial filtration using peristaltic pump sample name LV_56 sample storage duration 3 sample storage temperature -80 sampling campaign cruise sampling platform Research Vessel HCMR sampling site LV_st_18 scientific_name marine metagenome target gene 18S temperature 17 total depth of water column 3500 ERS2429925 SAMEA4609231 408172 marine metagenome Levantine Sea (Eastern Mediterranean); plankton; eukaryotic and prokaryotic communities composition; amplicons; illumina MiSeq; size fractionation by filtration DNA extraction method PowerWater DNA isolation kit ENA-FIRST-PUBLIC 2019-06-23T04:04:02Z ENA-LAST-UPDATE 2018-04-25T07:47:43Z External Id SAMEA4609231 INSDC center name HELLENIC CENTRE FOR MARINE RESEARCH INSDC first public 2019-06-23T04:04:02Z INSDC last update 2018-04-25T07:47:43Z INSDC status public Latitude End 34.4333 Longitude End 26.3833 Submitter Id LV_58 amount or size of sample collected 30 depth 3500 environment (biome) temperate mediterannean sea biome environment (feature) marine pelagic feature environment (material) planktonic material environmental package water event date/time 2016-04-12T22:00:00Z fraction_microns 20 latitude start 34.4333 longitude start 26.3833 marine region Levantine basin (eastern Mediterannean) primer_f 5'CCCTGCCHTTTGTACACAC primer_f_reference Amaral-Zettler LA, McCliment EA, Ducklow HW, Huse SM. (2009). A Method for Studying Protistan Diversity Using Massively Parallel Sequencing of V9 Hypervariable Regions of Small-Subunit Ribosomal RNA Genes. PLoS One 4: e6372. primer_r 5'CCTTCYGCAGGTTCACCTAC primer_r_reference Amaral-Zettler LA, McCliment EA, Ducklow HW, Huse SM. (2009). A Method for Studying Protistan Diversity Using Massively Parallel Sequencing of V9 Hypervariable Regions of Small-Subunit Ribosomal RNA Genes. PLoS One 4: e6372. project name LEVECO protocol label sequencial filtration and preservation salinity 38.75 sample collection device or method sequencial filtration using peristaltic pump sample name LV_58 sample storage duration 3 sample storage temperature -80 sampling campaign cruise sampling platform Research Vessel HCMR sampling site LV_st_18 scientific_name marine metagenome target gene 18S temperature 13.5 total depth of water column 3500 ERS2429926 SAMEA4609232 408172 marine metagenome Levantine Sea (Eastern Mediterranean); plankton; eukaryotic and prokaryotic communities composition; amplicons; illumina MiSeq; size fractionation by filtration DNA extraction method PowerWater DNA isolation kit ENA-FIRST-PUBLIC 2019-06-23T04:04:02Z ENA-LAST-UPDATE 2018-04-25T07:47:43Z External Id SAMEA4609232 INSDC center name HELLENIC CENTRE FOR MARINE RESEARCH INSDC first public 2019-06-23T04:04:02Z INSDC last update 2018-04-25T07:47:43Z INSDC status public Latitude End 34.4333 Longitude End 26.3833 Submitter Id LV_59 amount or size of sample collected 30 depth 3500 environment (biome) temperate mediterannean sea biome environment (feature) marine pelagic feature environment (material) planktonic material environmental package water event date/time 2016-04-12T22:00:00Z fraction_microns 0.8 latitude start 34.4333 longitude start 26.3833 marine region Levantine basin (eastern Mediterannean) primer_f 5'CCCTGCCHTTTGTACACAC primer_f_reference Amaral-Zettler LA, McCliment EA, Ducklow HW, Huse SM. (2009). A Method for Studying Protistan Diversity Using Massively Parallel Sequencing of V9 Hypervariable Regions of Small-Subunit Ribosomal RNA Genes. PLoS One 4: e6372. primer_r 5'CCTTCYGCAGGTTCACCTAC primer_r_reference Amaral-Zettler LA, McCliment EA, Ducklow HW, Huse SM. (2009). A Method for Studying Protistan Diversity Using Massively Parallel Sequencing of V9 Hypervariable Regions of Small-Subunit Ribosomal RNA Genes. PLoS One 4: e6372. project name LEVECO protocol label sequencial filtration and preservation salinity 38.75 sample collection device or method sequencial filtration using peristaltic pump sample name LV_59 sample storage duration 3 sample storage temperature -80 sampling campaign cruise sampling platform Research Vessel HCMR sampling site LV_st_18 scientific_name marine metagenome target gene 18S temperature 13.5 total depth of water column 3500 ERS2429928 SAMEA4609234 408172 marine metagenome Levantine Sea (Eastern Mediterranean); plankton; eukaryotic and prokaryotic communities composition; amplicons; illumina MiSeq; size fractionation by filtration DNA extraction method PowerWater DNA isolation kit ENA-FIRST-PUBLIC 2019-06-23T04:04:02Z ENA-LAST-UPDATE 2018-04-25T07:47:43Z External Id SAMEA4609234 INSDC center name HELLENIC CENTRE FOR MARINE RESEARCH INSDC first public 2019-06-23T04:04:02Z INSDC last update 2018-04-25T07:47:43Z INSDC status public Latitude End 35.0333 Longitude End 23.4667 Submitter Id LV_61 amount or size of sample collected 10 depth 0 environment (biome) temperate mediterannean sea biome environment (feature) marine pelagic feature environment (material) planktonic material environmental package water event date/time 2016-04-17T23:30:00Z fraction_microns 20 latitude start 35.0333 longitude start 23.4667 marine region Levantine basin (eastern Mediterannean) primer_f 5'CCCTGCCHTTTGTACACAC primer_f_reference Amaral-Zettler LA, McCliment EA, Ducklow HW, Huse SM. (2009). A Method for Studying Protistan Diversity Using Massively Parallel Sequencing of V9 Hypervariable Regions of Small-Subunit Ribosomal RNA Genes. PLoS One 4: e6372. primer_r 5'CCTTCYGCAGGTTCACCTAC primer_r_reference Amaral-Zettler LA, McCliment EA, Ducklow HW, Huse SM. (2009). A Method for Studying Protistan Diversity Using Massively Parallel Sequencing of V9 Hypervariable Regions of Small-Subunit Ribosomal RNA Genes. PLoS One 4: e6372. project name LEVECO protocol label sequencial filtration and preservation salinity 0 sample collection device or method sequencial filtration using peristaltic pump sample name LV_61 sample storage duration 3 sample storage temperature -80 sampling campaign cruise sampling platform Research Vessel HCMR sampling site LV_Blank scientific_name marine metagenome target gene 18S temperature 25 total depth of water column 0 ERS2429929 SAMEA4609235 408172 marine metagenome Levantine Sea (Eastern Mediterranean); plankton; eukaryotic and prokaryotic communities composition; amplicons; illumina MiSeq; size fractionation by filtration DNA extraction method PowerWater DNA isolation kit ENA-FIRST-PUBLIC 2019-06-23T04:04:02Z ENA-LAST-UPDATE 2018-04-25T07:47:43Z External Id SAMEA4609235 INSDC center name HELLENIC CENTRE FOR MARINE RESEARCH INSDC first public 2019-06-23T04:04:02Z INSDC last update 2018-04-25T07:47:43Z INSDC status public Latitude End 35.0333 Longitude End 23.4667 Submitter Id LV_62 amount or size of sample collected 10 depth 0 environment (biome) temperate mediterannean sea biome environment (feature) marine pelagic feature environment (material) planktonic material environmental package water event date/time 2016-04-17T23:30:00Z fraction_microns 0.8 latitude start 35.0333 longitude start 23.4667 marine region Levantine basin (eastern Mediterannean) primer_f 5'CCCTGCCHTTTGTACACAC primer_f_reference Amaral-Zettler LA, McCliment EA, Ducklow HW, Huse SM. (2009). A Method for Studying Protistan Diversity Using Massively Parallel Sequencing of V9 Hypervariable Regions of Small-Subunit Ribosomal RNA Genes. PLoS One 4: e6372. primer_r 5'CCTTCYGCAGGTTCACCTAC primer_r_reference Amaral-Zettler LA, McCliment EA, Ducklow HW, Huse SM. (2009). A Method for Studying Protistan Diversity Using Massively Parallel Sequencing of V9 Hypervariable Regions of Small-Subunit Ribosomal RNA Genes. PLoS One 4: e6372. project name LEVECO protocol label sequencial filtration and preservation salinity 0 sample collection device or method sequencial filtration using peristaltic pump sample name LV_62 sample storage duration 3 sample storage temperature -80 sampling campaign cruise sampling platform Research Vessel HCMR sampling site LV_Blank scientific_name marine metagenome target gene 18S temperature 25 total depth of water column 0 ERS2429899 SAMEA4609205 408172 marine metagenome Levantine Sea (Eastern Mediterranean); plankton; eukaryotic and prokaryotic communities composition; amplicons; illumina MiSeq; size fractionation by filtration DNA extraction method PowerWater DNA isolation kit ENA-FIRST-PUBLIC 2019-06-23T04:04:02Z ENA-LAST-UPDATE 2018-04-25T07:47:42Z External Id SAMEA4609205 INSDC center name HELLENIC CENTRE FOR MARINE RESEARCH INSDC first public 2019-06-23T04:04:02Z INSDC last update 2018-04-25T07:47:42Z INSDC status public Latitude End 34.25 Longitude End 25.4833 Submitter Id LV_32 amount or size of sample collected 30 depth 1000 environment (biome) temperate mediterannean sea biome environment (feature) marine pelagic feature environment (material) planktonic material environmental package water event date/time 2016-04-14T09:30:00Z fraction_microns 0.8 latitude start 34.25 longitude start 25.4833 marine region Levantine basin (eastern Mediterannean) primer_f 5'CCCTGCCHTTTGTACACAC primer_f_reference Amaral-Zettler LA, McCliment EA, Ducklow HW, Huse SM. (2009). A Method for Studying Protistan Diversity Using Massively Parallel Sequencing of V9 Hypervariable Regions of Small-Subunit Ribosomal RNA Genes. PLoS One 4: e6372. primer_r 5'CCTTCYGCAGGTTCACCTAC primer_r_reference Amaral-Zettler LA, McCliment EA, Ducklow HW, Huse SM. (2009). A Method for Studying Protistan Diversity Using Massively Parallel Sequencing of V9 Hypervariable Regions of Small-Subunit Ribosomal RNA Genes. PLoS One 4: e6372. project name LEVECO protocol label sequencial filtration and preservation salinity 38.78 sample collection device or method sequencial filtration using peristaltic pump sample name LV_32 sample storage duration 3 sample storage temperature -80 sampling campaign cruise sampling platform Research Vessel HCMR sampling site LV_st_13 scientific_name marine metagenome target gene 18S temperature 13.7 total depth of water column 3300