ERX9332061 E-MTAB-11756:Adenolobus_pechuelii_82389_1_p ERP137928 PRJEB53148 Transcriptome sequencing of divergent Fabales ERS12080436 SAMEA14456150 Adenolobus_pechuelii_82389_1_p RNA-Seq TRANSCRIPTOMIC Oligo-dT The seeds were obtained from U.S. National Plant Germplasm System Seeds were germinated in Petri dishes in the temperature optimal for each species and transferred to the pots in the cultivation room (photoperiod: 16h/8h, temperature: 22°C, humidity: 70 – 80%). No symbiotic bacteria was added to the soil. RNA isolation was carried out according to the manufacturer's protocol of Spectrum Plant Total RNA Kit (Sigma Aldrich) with minor modifications. Optimization of the protocol included decreasing the amount of ground tissue used to extraction (50 mg instead of proposed 100 mg). The incubation was performed for 5 min at 56°C. Moreover, 750 μL of the binding solution (instead of the proposed 500μL) was added. The DNase digestion was incorporated with the use of the protocol On-Column DNase I Digestion Set (Sigma Aldrich). RNA concentration and quality was measured with the use of Experion Automated Electrophoresis System, RNA StdSens Analysis Kit (Bio-Rad).High-quality DNA was isolated with the use of DNeasy Plant Pro Kit (Qiagen) with no changes to the protocol made. DNA yield has been estimated by nanodrop (ThermoFisher) and quality on 2% agarose gel. RNA libraries were prepared using TruSeq RNA Stranded mRNA. Illumina NovaSeq 6000 Experimental Factor: organism Adenolobus peuchelii ERX9332062 E-MTAB-11756:Adenolobus_pechuelii_82389_2_p ERP137928 PRJEB53148 Transcriptome sequencing of divergent Fabales ERS12080437 SAMEA14456151 Adenolobus_pechuelii_82389_2_p RNA-Seq TRANSCRIPTOMIC Oligo-dT The seeds were obtained from U.S. National Plant Germplasm System Seeds were germinated in Petri dishes in the temperature optimal for each species and transferred to the pots in the cultivation room (photoperiod: 16h/8h, temperature: 22°C, humidity: 70 – 80%). No symbiotic bacteria was added to the soil. RNA isolation was carried out according to the manufacturer's protocol of Spectrum Plant Total RNA Kit (Sigma Aldrich) with minor modifications. Optimization of the protocol included decreasing the amount of ground tissue used to extraction (50 mg instead of proposed 100 mg). The incubation was performed for 5 min at 56°C. Moreover, 750 μL of the binding solution (instead of the proposed 500μL) was added. The DNase digestion was incorporated with the use of the protocol On-Column DNase I Digestion Set (Sigma Aldrich). RNA concentration and quality was measured with the use of Experion Automated Electrophoresis System, RNA StdSens Analysis Kit (Bio-Rad).High-quality DNA was isolated with the use of DNeasy Plant Pro Kit (Qiagen) with no changes to the protocol made. DNA yield has been estimated by nanodrop (ThermoFisher) and quality on 2% agarose gel. RNA libraries were prepared using TruSeq RNA Stranded mRNA. Illumina NovaSeq 6000 Experimental Factor: organism Adenolobus peuchelii ERX9332063 E-MTAB-11756:Adenolobus_pechuelii_82389_3_p ERP137928 PRJEB53148 Transcriptome sequencing of divergent Fabales ERS12080438 SAMEA14456152 Adenolobus_pechuelii_82389_3_p RNA-Seq TRANSCRIPTOMIC Oligo-dT The seeds were obtained from U.S. National Plant Germplasm System Seeds were germinated in Petri dishes in the temperature optimal for each species and transferred to the pots in the cultivation room (photoperiod: 16h/8h, temperature: 22°C, humidity: 70 – 80%). No symbiotic bacteria was added to the soil. RNA isolation was carried out according to the manufacturer's protocol of Spectrum Plant Total RNA Kit (Sigma Aldrich) with minor modifications. Optimization of the protocol included decreasing the amount of ground tissue used to extraction (50 mg instead of proposed 100 mg). The incubation was performed for 5 min at 56°C. Moreover, 750 μL of the binding solution (instead of the proposed 500μL) was added. The DNase digestion was incorporated with the use of the protocol On-Column DNase I Digestion Set (Sigma Aldrich). RNA concentration and quality was measured with the use of Experion Automated Electrophoresis System, RNA StdSens Analysis Kit (Bio-Rad).High-quality DNA was isolated with the use of DNeasy Plant Pro Kit (Qiagen) with no changes to the protocol made. DNA yield has been estimated by nanodrop (ThermoFisher) and quality on 2% agarose gel. RNA libraries were prepared using TruSeq RNA Stranded mRNA. Illumina NovaSeq 6000 Experimental Factor: organism Adenolobus peuchelii ERX9332064 E-MTAB-11756:Cassia_sturtii_209803_p ERP137928 PRJEB53148 Transcriptome sequencing of divergent Fabales ERS12080439 SAMEA14456153 Cassia_sturtii_209803_p RNA-Seq TRANSCRIPTOMIC Oligo-dT The seeds were obtained from U.S. National Plant Germplasm System Seeds were germinated in Petri dishes in the temperature optimal for each species and transferred to the pots in the cultivation room (photoperiod: 16h/8h, temperature: 22°C, humidity: 70 – 80%). No symbiotic bacteria was added to the soil. RNA isolation was carried out according to the manufacturer's protocol of Spectrum Plant Total RNA Kit (Sigma Aldrich) with minor modifications. Optimization of the protocol included decreasing the amount of ground tissue used to extraction (50 mg instead of proposed 100 mg). The incubation was performed for 5 min at 56°C. Moreover, 750 μL of the binding solution (instead of the proposed 500μL) was added. The DNase digestion was incorporated with the use of the protocol On-Column DNase I Digestion Set (Sigma Aldrich). RNA concentration and quality was measured with the use of Experion Automated Electrophoresis System, RNA StdSens Analysis Kit (Bio-Rad).High-quality DNA was isolated with the use of DNeasy Plant Pro Kit (Qiagen) with no changes to the protocol made. DNA yield has been estimated by nanodrop (ThermoFisher) and quality on 2% agarose gel. RNA libraries were prepared using TruSeq RNA Stranded mRNA. Illumina NovaSeq 6000 Experimental Factor: organism Cassia sturtii ERX9332065 E-MTAB-11756:Cassia_sturtii_209803_2_p ERP137928 PRJEB53148 Transcriptome sequencing of divergent Fabales ERS12080440 SAMEA14456154 Cassia_sturtii_209803_2_p RNA-Seq TRANSCRIPTOMIC Oligo-dT The seeds were obtained from U.S. National Plant Germplasm System Seeds were germinated in Petri dishes in the temperature optimal for each species and transferred to the pots in the cultivation room (photoperiod: 16h/8h, temperature: 22°C, humidity: 70 – 80%). No symbiotic bacteria was added to the soil. RNA isolation was carried out according to the manufacturer's protocol of Spectrum Plant Total RNA Kit (Sigma Aldrich) with minor modifications. Optimization of the protocol included decreasing the amount of ground tissue used to extraction (50 mg instead of proposed 100 mg). The incubation was performed for 5 min at 56°C. Moreover, 750 μL of the binding solution (instead of the proposed 500μL) was added. The DNase digestion was incorporated with the use of the protocol On-Column DNase I Digestion Set (Sigma Aldrich). RNA concentration and quality was measured with the use of Experion Automated Electrophoresis System, RNA StdSens Analysis Kit (Bio-Rad).High-quality DNA was isolated with the use of DNeasy Plant Pro Kit (Qiagen) with no changes to the protocol made. DNA yield has been estimated by nanodrop (ThermoFisher) and quality on 2% agarose gel. RNA libraries were prepared using TruSeq RNA Stranded mRNA. Illumina NovaSeq 6000 Experimental Factor: organism Cassia sturtii ERX9332066 E-MTAB-11756:Cassia_sturtii_209803_3_p ERP137928 PRJEB53148 Transcriptome sequencing of divergent Fabales ERS12080441 SAMEA14456155 Cassia_sturtii_209803_3_p RNA-Seq TRANSCRIPTOMIC Oligo-dT The seeds were obtained from U.S. National Plant Germplasm System Seeds were germinated in Petri dishes in the temperature optimal for each species and transferred to the pots in the cultivation room (photoperiod: 16h/8h, temperature: 22°C, humidity: 70 – 80%). No symbiotic bacteria was added to the soil. RNA isolation was carried out according to the manufacturer's protocol of Spectrum Plant Total RNA Kit (Sigma Aldrich) with minor modifications. Optimization of the protocol included decreasing the amount of ground tissue used to extraction (50 mg instead of proposed 100 mg). The incubation was performed for 5 min at 56°C. Moreover, 750 μL of the binding solution (instead of the proposed 500μL) was added. The DNase digestion was incorporated with the use of the protocol On-Column DNase I Digestion Set (Sigma Aldrich). RNA concentration and quality was measured with the use of Experion Automated Electrophoresis System, RNA StdSens Analysis Kit (Bio-Rad).High-quality DNA was isolated with the use of DNeasy Plant Pro Kit (Qiagen) with no changes to the protocol made. DNA yield has been estimated by nanodrop (ThermoFisher) and quality on 2% agarose gel. RNA libraries were prepared using TruSeq RNA Stranded mRNA. Illumina NovaSeq 6000 Experimental Factor: organism Cassia sturtii ERX9332067 E-MTAB-11756:Ceratonia_siliqua_165598_p ERP137928 PRJEB53148 Transcriptome sequencing of divergent Fabales ERS12080442 SAMEA14456156 Ceratonia_siliqua_165598_p RNA-Seq TRANSCRIPTOMIC Oligo-dT The seeds were obtained from U.S. National Plant Germplasm System Seeds were germinated in Petri dishes in the temperature optimal for each species and transferred to the pots in the cultivation room (photoperiod: 16h/8h, temperature: 22°C, humidity: 70 – 80%). No symbiotic bacteria was added to the soil. RNA isolation was carried out according to the manufacturer's protocol of Spectrum Plant Total RNA Kit (Sigma Aldrich) with minor modifications. Optimization of the protocol included decreasing the amount of ground tissue used to extraction (50 mg instead of proposed 100 mg). The incubation was performed for 5 min at 56°C. Moreover, 750 μL of the binding solution (instead of the proposed 500μL) was added. The DNase digestion was incorporated with the use of the protocol On-Column DNase I Digestion Set (Sigma Aldrich). RNA concentration and quality was measured with the use of Experion Automated Electrophoresis System, RNA StdSens Analysis Kit (Bio-Rad).High-quality DNA was isolated with the use of DNeasy Plant Pro Kit (Qiagen) with no changes to the protocol made. DNA yield has been estimated by nanodrop (ThermoFisher) and quality on 2% agarose gel. RNA libraries were prepared using TruSeq RNA Stranded mRNA. Illumina NovaSeq 6000 Experimental Factor: organism Ceratonia siliqua ERX9332068 E-MTAB-11756:Ceratonia_siliqua_165598_2_p ERP137928 PRJEB53148 Transcriptome sequencing of divergent Fabales ERS12080443 SAMEA14456157 Ceratonia_siliqua_165598_2_p RNA-Seq TRANSCRIPTOMIC Oligo-dT The seeds were obtained from U.S. National Plant Germplasm System Seeds were germinated in Petri dishes in the temperature optimal for each species and transferred to the pots in the cultivation room (photoperiod: 16h/8h, temperature: 22°C, humidity: 70 – 80%). No symbiotic bacteria was added to the soil. RNA isolation was carried out according to the manufacturer's protocol of Spectrum Plant Total RNA Kit (Sigma Aldrich) with minor modifications. Optimization of the protocol included decreasing the amount of ground tissue used to extraction (50 mg instead of proposed 100 mg). The incubation was performed for 5 min at 56°C. Moreover, 750 μL of the binding solution (instead of the proposed 500μL) was added. The DNase digestion was incorporated with the use of the protocol On-Column DNase I Digestion Set (Sigma Aldrich). RNA concentration and quality was measured with the use of Experion Automated Electrophoresis System, RNA StdSens Analysis Kit (Bio-Rad).High-quality DNA was isolated with the use of DNeasy Plant Pro Kit (Qiagen) with no changes to the protocol made. DNA yield has been estimated by nanodrop (ThermoFisher) and quality on 2% agarose gel. RNA libraries were prepared using TruSeq RNA Stranded mRNA. Illumina NovaSeq 6000 Experimental Factor: organism Ceratonia siliqua ERX9332069 E-MTAB-11756:Ceratonia_siliqua_165598_3_p ERP137928 PRJEB53148 Transcriptome sequencing of divergent Fabales ERS12080444 SAMEA14456158 Ceratonia_siliqua_165598_3_p RNA-Seq TRANSCRIPTOMIC Oligo-dT The seeds were obtained from U.S. National Plant Germplasm System Seeds were germinated in Petri dishes in the temperature optimal for each species and transferred to the pots in the cultivation room (photoperiod: 16h/8h, temperature: 22°C, humidity: 70 – 80%). No symbiotic bacteria was added to the soil. RNA isolation was carried out according to the manufacturer's protocol of Spectrum Plant Total RNA Kit (Sigma Aldrich) with minor modifications. Optimization of the protocol included decreasing the amount of ground tissue used to extraction (50 mg instead of proposed 100 mg). The incubation was performed for 5 min at 56°C. Moreover, 750 μL of the binding solution (instead of the proposed 500μL) was added. The DNase digestion was incorporated with the use of the protocol On-Column DNase I Digestion Set (Sigma Aldrich). RNA concentration and quality was measured with the use of Experion Automated Electrophoresis System, RNA StdSens Analysis Kit (Bio-Rad).High-quality DNA was isolated with the use of DNeasy Plant Pro Kit (Qiagen) with no changes to the protocol made. DNA yield has been estimated by nanodrop (ThermoFisher) and quality on 2% agarose gel. RNA libraries were prepared using TruSeq RNA Stranded mRNA. Illumina NovaSeq 6000 Experimental Factor: organism Ceratonia siliqua ERX9332070 E-MTAB-11756:Chamaecrista_mimosoides_81072_p ERP137928 PRJEB53148 Transcriptome sequencing of divergent Fabales ERS12080445 SAMEA14456159 Chamaecrista_mimosoides_81072_p RNA-Seq TRANSCRIPTOMIC Oligo-dT The seeds were obtained from U.S. National Plant Germplasm System Seeds were germinated in Petri dishes in the temperature optimal for each species and transferred to the pots in the cultivation room (photoperiod: 16h/8h, temperature: 22°C, humidity: 70 – 80%). No symbiotic bacteria was added to the soil. RNA isolation was carried out according to the manufacturer's protocol of Spectrum Plant Total RNA Kit (Sigma Aldrich) with minor modifications. Optimization of the protocol included decreasing the amount of ground tissue used to extraction (50 mg instead of proposed 100 mg). The incubation was performed for 5 min at 56°C. Moreover, 750 μL of the binding solution (instead of the proposed 500μL) was added. The DNase digestion was incorporated with the use of the protocol On-Column DNase I Digestion Set (Sigma Aldrich). RNA concentration and quality was measured with the use of Experion Automated Electrophoresis System, RNA StdSens Analysis Kit (Bio-Rad).High-quality DNA was isolated with the use of DNeasy Plant Pro Kit (Qiagen) with no changes to the protocol made. DNA yield has been estimated by nanodrop (ThermoFisher) and quality on 2% agarose gel. RNA libraries were prepared using TruSeq RNA Stranded mRNA. Illumina NovaSeq 6000 Experimental Factor: organism Chamaecrista mimosoides ERX9332071 E-MTAB-11756:Chamaecrista_mimosoides_81072_2_p ERP137928 PRJEB53148 Transcriptome sequencing of divergent Fabales ERS12080446 SAMEA14456160 Chamaecrista_mimosoides_81072_2_p RNA-Seq TRANSCRIPTOMIC Oligo-dT The seeds were obtained from U.S. National Plant Germplasm System Seeds were germinated in Petri dishes in the temperature optimal for each species and transferred to the pots in the cultivation room (photoperiod: 16h/8h, temperature: 22°C, humidity: 70 – 80%). No symbiotic bacteria was added to the soil. RNA isolation was carried out according to the manufacturer's protocol of Spectrum Plant Total RNA Kit (Sigma Aldrich) with minor modifications. Optimization of the protocol included decreasing the amount of ground tissue used to extraction (50 mg instead of proposed 100 mg). The incubation was performed for 5 min at 56°C. Moreover, 750 μL of the binding solution (instead of the proposed 500μL) was added. The DNase digestion was incorporated with the use of the protocol On-Column DNase I Digestion Set (Sigma Aldrich). RNA concentration and quality was measured with the use of Experion Automated Electrophoresis System, RNA StdSens Analysis Kit (Bio-Rad).High-quality DNA was isolated with the use of DNeasy Plant Pro Kit (Qiagen) with no changes to the protocol made. DNA yield has been estimated by nanodrop (ThermoFisher) and quality on 2% agarose gel. RNA libraries were prepared using TruSeq RNA Stranded mRNA. Illumina NovaSeq 6000 Experimental Factor: organism Chamaecrista mimosoides ERX9332072 E-MTAB-11756:Chamaecrista_mimosoides_81072_3_p ERP137928 PRJEB53148 Transcriptome sequencing of divergent Fabales ERS12080447 SAMEA14456161 Chamaecrista_mimosoides_81072_3_p RNA-Seq TRANSCRIPTOMIC Oligo-dT The seeds were obtained from U.S. National Plant Germplasm System Seeds were germinated in Petri dishes in the temperature optimal for each species and transferred to the pots in the cultivation room (photoperiod: 16h/8h, temperature: 22°C, humidity: 70 – 80%). No symbiotic bacteria was added to the soil. RNA isolation was carried out according to the manufacturer's protocol of Spectrum Plant Total RNA Kit (Sigma Aldrich) with minor modifications. Optimization of the protocol included decreasing the amount of ground tissue used to extraction (50 mg instead of proposed 100 mg). The incubation was performed for 5 min at 56°C. Moreover, 750 μL of the binding solution (instead of the proposed 500μL) was added. The DNase digestion was incorporated with the use of the protocol On-Column DNase I Digestion Set (Sigma Aldrich). RNA concentration and quality was measured with the use of Experion Automated Electrophoresis System, RNA StdSens Analysis Kit (Bio-Rad).High-quality DNA was isolated with the use of DNeasy Plant Pro Kit (Qiagen) with no changes to the protocol made. DNA yield has been estimated by nanodrop (ThermoFisher) and quality on 2% agarose gel. RNA libraries were prepared using TruSeq RNA Stranded mRNA. Illumina NovaSeq 6000 Experimental Factor: organism Chamaecrista mimosoides ERX9332073 E-MTAB-11756:Desmanthus_velutinus_326922_p ERP137928 PRJEB53148 Transcriptome sequencing of divergent Fabales ERS12080448 SAMEA14456162 Desmanthus_velutinus_326922_p RNA-Seq TRANSCRIPTOMIC Oligo-dT The seeds were obtained from U.S. National Plant Germplasm System Seeds were germinated in Petri dishes in the temperature optimal for each species and transferred to the pots in the cultivation room (photoperiod: 16h/8h, temperature: 22°C, humidity: 70 – 80%). No symbiotic bacteria was added to the soil. RNA isolation was carried out according to the manufacturer's protocol of Spectrum Plant Total RNA Kit (Sigma Aldrich) with minor modifications. Optimization of the protocol included decreasing the amount of ground tissue used to extraction (50 mg instead of proposed 100 mg). The incubation was performed for 5 min at 56°C. Moreover, 750 μL of the binding solution (instead of the proposed 500μL) was added. The DNase digestion was incorporated with the use of the protocol On-Column DNase I Digestion Set (Sigma Aldrich). RNA concentration and quality was measured with the use of Experion Automated Electrophoresis System, RNA StdSens Analysis Kit (Bio-Rad).High-quality DNA was isolated with the use of DNeasy Plant Pro Kit (Qiagen) with no changes to the protocol made. DNA yield has been estimated by nanodrop (ThermoFisher) and quality on 2% agarose gel. RNA libraries were prepared using TruSeq RNA Stranded mRNA. Illumina NovaSeq 6000 Experimental Factor: organism Desmanthus velutinus ERX9332074 E-MTAB-11756:Desmanthus_velutinus_326922_2_p ERP137928 PRJEB53148 Transcriptome sequencing of divergent Fabales ERS12080449 SAMEA14456163 Desmanthus_velutinus_326922_2_p RNA-Seq TRANSCRIPTOMIC Oligo-dT The seeds were obtained from U.S. National Plant Germplasm System Seeds were germinated in Petri dishes in the temperature optimal for each species and transferred to the pots in the cultivation room (photoperiod: 16h/8h, temperature: 22°C, humidity: 70 – 80%). No symbiotic bacteria was added to the soil. RNA isolation was carried out according to the manufacturer's protocol of Spectrum Plant Total RNA Kit (Sigma Aldrich) with minor modifications. Optimization of the protocol included decreasing the amount of ground tissue used to extraction (50 mg instead of proposed 100 mg). The incubation was performed for 5 min at 56°C. Moreover, 750 μL of the binding solution (instead of the proposed 500μL) was added. The DNase digestion was incorporated with the use of the protocol On-Column DNase I Digestion Set (Sigma Aldrich). RNA concentration and quality was measured with the use of Experion Automated Electrophoresis System, RNA StdSens Analysis Kit (Bio-Rad).High-quality DNA was isolated with the use of DNeasy Plant Pro Kit (Qiagen) with no changes to the protocol made. DNA yield has been estimated by nanodrop (ThermoFisher) and quality on 2% agarose gel. RNA libraries were prepared using TruSeq RNA Stranded mRNA. Illumina NovaSeq 6000 Experimental Factor: organism Desmanthus velutinus ERX9332075 E-MTAB-11756:Desmanthus_velutinus_326922_3_p ERP137928 PRJEB53148 Transcriptome sequencing of divergent Fabales ERS12080450 SAMEA14456164 Desmanthus_velutinus_326922_3_p RNA-Seq TRANSCRIPTOMIC Oligo-dT The seeds were obtained from U.S. National Plant Germplasm System Seeds were germinated in Petri dishes in the temperature optimal for each species and transferred to the pots in the cultivation room (photoperiod: 16h/8h, temperature: 22°C, humidity: 70 – 80%). No symbiotic bacteria was added to the soil. RNA isolation was carried out according to the manufacturer's protocol of Spectrum Plant Total RNA Kit (Sigma Aldrich) with minor modifications. Optimization of the protocol included decreasing the amount of ground tissue used to extraction (50 mg instead of proposed 100 mg). The incubation was performed for 5 min at 56°C. Moreover, 750 μL of the binding solution (instead of the proposed 500μL) was added. The DNase digestion was incorporated with the use of the protocol On-Column DNase I Digestion Set (Sigma Aldrich). RNA concentration and quality was measured with the use of Experion Automated Electrophoresis System, RNA StdSens Analysis Kit (Bio-Rad).High-quality DNA was isolated with the use of DNeasy Plant Pro Kit (Qiagen) with no changes to the protocol made. DNA yield has been estimated by nanodrop (ThermoFisher) and quality on 2% agarose gel. RNA libraries were prepared using TruSeq RNA Stranded mRNA. Illumina NovaSeq 6000 Experimental Factor: organism Desmanthus velutinus ERX9332076 E-MTAB-11756:Dichrostachys_cinerea_171155_p ERP137928 PRJEB53148 Transcriptome sequencing of divergent Fabales ERS12080451 SAMEA14456165 Dichrostachys_cinerea_171155_p RNA-Seq TRANSCRIPTOMIC Oligo-dT The seeds were obtained from U.S. National Plant Germplasm System Seeds were germinated in Petri dishes in the temperature optimal for each species and transferred to the pots in the cultivation room (photoperiod: 16h/8h, temperature: 22°C, humidity: 70 – 80%). No symbiotic bacteria was added to the soil. RNA isolation was carried out according to the manufacturer's protocol of Spectrum Plant Total RNA Kit (Sigma Aldrich) with minor modifications. Optimization of the protocol included decreasing the amount of ground tissue used to extraction (50 mg instead of proposed 100 mg). The incubation was performed for 5 min at 56°C. Moreover, 750 μL of the binding solution (instead of the proposed 500μL) was added. The DNase digestion was incorporated with the use of the protocol On-Column DNase I Digestion Set (Sigma Aldrich). RNA concentration and quality was measured with the use of Experion Automated Electrophoresis System, RNA StdSens Analysis Kit (Bio-Rad).High-quality DNA was isolated with the use of DNeasy Plant Pro Kit (Qiagen) with no changes to the protocol made. DNA yield has been estimated by nanodrop (ThermoFisher) and quality on 2% agarose gel. RNA libraries were prepared using TruSeq RNA Stranded mRNA. Illumina NovaSeq 6000 Experimental Factor: organism Dichrostachys cinerea ERX9332077 E-MTAB-11756:Dichrostachys_cinerea_171155_2_p ERP137928 PRJEB53148 Transcriptome sequencing of divergent Fabales ERS12080452 SAMEA14456166 Dichrostachys_cinerea_171155_2_p RNA-Seq TRANSCRIPTOMIC Oligo-dT The seeds were obtained from U.S. National Plant Germplasm System Seeds were germinated in Petri dishes in the temperature optimal for each species and transferred to the pots in the cultivation room (photoperiod: 16h/8h, temperature: 22°C, humidity: 70 – 80%). No symbiotic bacteria was added to the soil. RNA isolation was carried out according to the manufacturer's protocol of Spectrum Plant Total RNA Kit (Sigma Aldrich) with minor modifications. Optimization of the protocol included decreasing the amount of ground tissue used to extraction (50 mg instead of proposed 100 mg). The incubation was performed for 5 min at 56°C. Moreover, 750 μL of the binding solution (instead of the proposed 500μL) was added. The DNase digestion was incorporated with the use of the protocol On-Column DNase I Digestion Set (Sigma Aldrich). RNA concentration and quality was measured with the use of Experion Automated Electrophoresis System, RNA StdSens Analysis Kit (Bio-Rad).High-quality DNA was isolated with the use of DNeasy Plant Pro Kit (Qiagen) with no changes to the protocol made. DNA yield has been estimated by nanodrop (ThermoFisher) and quality on 2% agarose gel. RNA libraries were prepared using TruSeq RNA Stranded mRNA. Illumina NovaSeq 6000 Experimental Factor: organism Dichrostachys cinerea ERX9332078 E-MTAB-11756:Dichrostachys_cinerea_171155_3_p ERP137928 PRJEB53148 Transcriptome sequencing of divergent Fabales ERS12080453 SAMEA14456167 Dichrostachys_cinerea_171155_3_p RNA-Seq TRANSCRIPTOMIC Oligo-dT The seeds were obtained from U.S. National Plant Germplasm System Seeds were germinated in Petri dishes in the temperature optimal for each species and transferred to the pots in the cultivation room (photoperiod: 16h/8h, temperature: 22°C, humidity: 70 – 80%). No symbiotic bacteria was added to the soil. RNA isolation was carried out according to the manufacturer's protocol of Spectrum Plant Total RNA Kit (Sigma Aldrich) with minor modifications. Optimization of the protocol included decreasing the amount of ground tissue used to extraction (50 mg instead of proposed 100 mg). The incubation was performed for 5 min at 56°C. Moreover, 750 μL of the binding solution (instead of the proposed 500μL) was added. The DNase digestion was incorporated with the use of the protocol On-Column DNase I Digestion Set (Sigma Aldrich). RNA concentration and quality was measured with the use of Experion Automated Electrophoresis System, RNA StdSens Analysis Kit (Bio-Rad).High-quality DNA was isolated with the use of DNeasy Plant Pro Kit (Qiagen) with no changes to the protocol made. DNA yield has been estimated by nanodrop (ThermoFisher) and quality on 2% agarose gel. RNA libraries were prepared using TruSeq RNA Stranded mRNA. Illumina NovaSeq 6000 Experimental Factor: organism Dichrostachys cinerea ERX9332079 E-MTAB-11756:Faidherbia_albida_104805_p ERP137928 PRJEB53148 Transcriptome sequencing of divergent Fabales ERS12080454 SAMEA14456168 Faidherbia_albida_104805_p RNA-Seq TRANSCRIPTOMIC Oligo-dT The seeds were obtained from U.S. National Plant Germplasm System Seeds were germinated in Petri dishes in the temperature optimal for each species and transferred to the pots in the cultivation room (photoperiod: 16h/8h, temperature: 22°C, humidity: 70 – 80%). No symbiotic bacteria was added to the soil. RNA isolation was carried out according to the manufacturer's protocol of Spectrum Plant Total RNA Kit (Sigma Aldrich) with minor modifications. Optimization of the protocol included decreasing the amount of ground tissue used to extraction (50 mg instead of proposed 100 mg). The incubation was performed for 5 min at 56°C. Moreover, 750 μL of the binding solution (instead of the proposed 500μL) was added. The DNase digestion was incorporated with the use of the protocol On-Column DNase I Digestion Set (Sigma Aldrich). RNA concentration and quality was measured with the use of Experion Automated Electrophoresis System, RNA StdSens Analysis Kit (Bio-Rad).High-quality DNA was isolated with the use of DNeasy Plant Pro Kit (Qiagen) with no changes to the protocol made. DNA yield has been estimated by nanodrop (ThermoFisher) and quality on 2% agarose gel. RNA libraries were prepared using TruSeq RNA Stranded mRNA. Illumina NovaSeq 6000 Experimental Factor: organism Faidherbia albida ERX9332080 E-MTAB-11756:Faidherbia_albida_104805_2_p ERP137928 PRJEB53148 Transcriptome sequencing of divergent Fabales ERS12080455 SAMEA14456169 Faidherbia_albida_104805_2_p RNA-Seq TRANSCRIPTOMIC Oligo-dT The seeds were obtained from U.S. National Plant Germplasm System Seeds were germinated in Petri dishes in the temperature optimal for each species and transferred to the pots in the cultivation room (photoperiod: 16h/8h, temperature: 22°C, humidity: 70 – 80%). No symbiotic bacteria was added to the soil. RNA isolation was carried out according to the manufacturer's protocol of Spectrum Plant Total RNA Kit (Sigma Aldrich) with minor modifications. Optimization of the protocol included decreasing the amount of ground tissue used to extraction (50 mg instead of proposed 100 mg). The incubation was performed for 5 min at 56°C. Moreover, 750 μL of the binding solution (instead of the proposed 500μL) was added. The DNase digestion was incorporated with the use of the protocol On-Column DNase I Digestion Set (Sigma Aldrich). RNA concentration and quality was measured with the use of Experion Automated Electrophoresis System, RNA StdSens Analysis Kit (Bio-Rad).High-quality DNA was isolated with the use of DNeasy Plant Pro Kit (Qiagen) with no changes to the protocol made. DNA yield has been estimated by nanodrop (ThermoFisher) and quality on 2% agarose gel. RNA libraries were prepared using TruSeq RNA Stranded mRNA. Illumina NovaSeq 6000 Experimental Factor: organism Faidherbia albida ERX9332081 E-MTAB-11756:Faidherbia_albida_104805_3_p ERP137928 PRJEB53148 Transcriptome sequencing of divergent Fabales ERS12080456 SAMEA14456170 Faidherbia_albida_104805_3_p RNA-Seq TRANSCRIPTOMIC Oligo-dT The seeds were obtained from U.S. National Plant Germplasm System Seeds were germinated in Petri dishes in the temperature optimal for each species and transferred to the pots in the cultivation room (photoperiod: 16h/8h, temperature: 22°C, humidity: 70 – 80%). No symbiotic bacteria was added to the soil. RNA isolation was carried out according to the manufacturer's protocol of Spectrum Plant Total RNA Kit (Sigma Aldrich) with minor modifications. Optimization of the protocol included decreasing the amount of ground tissue used to extraction (50 mg instead of proposed 100 mg). The incubation was performed for 5 min at 56°C. Moreover, 750 μL of the binding solution (instead of the proposed 500μL) was added. The DNase digestion was incorporated with the use of the protocol On-Column DNase I Digestion Set (Sigma Aldrich). RNA concentration and quality was measured with the use of Experion Automated Electrophoresis System, RNA StdSens Analysis Kit (Bio-Rad).High-quality DNA was isolated with the use of DNeasy Plant Pro Kit (Qiagen) with no changes to the protocol made. DNA yield has been estimated by nanodrop (ThermoFisher) and quality on 2% agarose gel. RNA libraries were prepared using TruSeq RNA Stranded mRNA. Illumina NovaSeq 6000 Experimental Factor: organism Faidherbia albida ERX9332082 E-MTAB-11756:Gymnocladus_dioicus_Ames_2917_1_p ERP137928 PRJEB53148 Transcriptome sequencing of divergent Fabales ERS12080457 SAMEA14456171 Gymnocladus_dioicus_Ames_2917_1_p RNA-Seq TRANSCRIPTOMIC Oligo-dT The seeds were obtained from U.S. National Plant Germplasm System Seeds were germinated in Petri dishes in the temperature optimal for each species and transferred to the pots in the cultivation room (photoperiod: 16h/8h, temperature: 22°C, humidity: 70 – 80%). No symbiotic bacteria was added to the soil. RNA isolation was carried out according to the manufacturer's protocol of Spectrum Plant Total RNA Kit (Sigma Aldrich) with minor modifications. Optimization of the protocol included decreasing the amount of ground tissue used to extraction (50 mg instead of proposed 100 mg). The incubation was performed for 5 min at 56°C. Moreover, 750 μL of the binding solution (instead of the proposed 500μL) was added. The DNase digestion was incorporated with the use of the protocol On-Column DNase I Digestion Set (Sigma Aldrich). RNA concentration and quality was measured with the use of Experion Automated Electrophoresis System, RNA StdSens Analysis Kit (Bio-Rad).High-quality DNA was isolated with the use of DNeasy Plant Pro Kit (Qiagen) with no changes to the protocol made. DNA yield has been estimated by nanodrop (ThermoFisher) and quality on 2% agarose gel. RNA libraries were prepared using TruSeq RNA Stranded mRNA. Illumina NovaSeq 6000 Experimental Factor: organism Gymnocladus dioicus ERX9332083 E-MTAB-11756:Gymnocladus_dioicus_Ames_2917_2_p ERP137928 PRJEB53148 Transcriptome sequencing of divergent Fabales ERS12080458 SAMEA14456172 Gymnocladus_dioicus_Ames_2917_2_p RNA-Seq TRANSCRIPTOMIC Oligo-dT The seeds were obtained from U.S. National Plant Germplasm System Seeds were germinated in Petri dishes in the temperature optimal for each species and transferred to the pots in the cultivation room (photoperiod: 16h/8h, temperature: 22°C, humidity: 70 – 80%). No symbiotic bacteria was added to the soil. RNA isolation was carried out according to the manufacturer's protocol of Spectrum Plant Total RNA Kit (Sigma Aldrich) with minor modifications. Optimization of the protocol included decreasing the amount of ground tissue used to extraction (50 mg instead of proposed 100 mg). The incubation was performed for 5 min at 56°C. Moreover, 750 μL of the binding solution (instead of the proposed 500μL) was added. The DNase digestion was incorporated with the use of the protocol On-Column DNase I Digestion Set (Sigma Aldrich). RNA concentration and quality was measured with the use of Experion Automated Electrophoresis System, RNA StdSens Analysis Kit (Bio-Rad).High-quality DNA was isolated with the use of DNeasy Plant Pro Kit (Qiagen) with no changes to the protocol made. DNA yield has been estimated by nanodrop (ThermoFisher) and quality on 2% agarose gel. RNA libraries were prepared using TruSeq RNA Stranded mRNA. Illumina NovaSeq 6000 Experimental Factor: organism Gymnocladus dioicus ERX9332084 E-MTAB-11756:Gymnocladus_dioicus_Ames_2917_3_p ERP137928 PRJEB53148 Transcriptome sequencing of divergent Fabales ERS12080459 SAMEA14456173 Gymnocladus_dioicus_Ames_2917_3_p RNA-Seq TRANSCRIPTOMIC Oligo-dT The seeds were obtained from U.S. National Plant Germplasm System Seeds were germinated in Petri dishes in the temperature optimal for each species and transferred to the pots in the cultivation room (photoperiod: 16h/8h, temperature: 22°C, humidity: 70 – 80%). No symbiotic bacteria was added to the soil. RNA isolation was carried out according to the manufacturer's protocol of Spectrum Plant Total RNA Kit (Sigma Aldrich) with minor modifications. Optimization of the protocol included decreasing the amount of ground tissue used to extraction (50 mg instead of proposed 100 mg). The incubation was performed for 5 min at 56°C. Moreover, 750 μL of the binding solution (instead of the proposed 500μL) was added. The DNase digestion was incorporated with the use of the protocol On-Column DNase I Digestion Set (Sigma Aldrich). RNA concentration and quality was measured with the use of Experion Automated Electrophoresis System, RNA StdSens Analysis Kit (Bio-Rad).High-quality DNA was isolated with the use of DNeasy Plant Pro Kit (Qiagen) with no changes to the protocol made. DNA yield has been estimated by nanodrop (ThermoFisher) and quality on 2% agarose gel. RNA libraries were prepared using TruSeq RNA Stranded mRNA. Illumina NovaSeq 6000 Experimental Factor: organism Gymnocladus dioicus ERX9332085 E-MTAB-11756:Piptadenia_stipulacea_102797_p ERP137928 PRJEB53148 Transcriptome sequencing of divergent Fabales ERS12080460 SAMEA14456174 Piptadenia_stipulacea_102797_p RNA-Seq TRANSCRIPTOMIC Oligo-dT The seeds were obtained from U.S. National Plant Germplasm System Seeds were germinated in Petri dishes in the temperature optimal for each species and transferred to the pots in the cultivation room (photoperiod: 16h/8h, temperature: 22°C, humidity: 70 – 80%). No symbiotic bacteria was added to the soil. RNA isolation was carried out according to the manufacturer's protocol of Spectrum Plant Total RNA Kit (Sigma Aldrich) with minor modifications. Optimization of the protocol included decreasing the amount of ground tissue used to extraction (50 mg instead of proposed 100 mg). The incubation was performed for 5 min at 56°C. Moreover, 750 μL of the binding solution (instead of the proposed 500μL) was added. The DNase digestion was incorporated with the use of the protocol On-Column DNase I Digestion Set (Sigma Aldrich). RNA concentration and quality was measured with the use of Experion Automated Electrophoresis System, RNA StdSens Analysis Kit (Bio-Rad).High-quality DNA was isolated with the use of DNeasy Plant Pro Kit (Qiagen) with no changes to the protocol made. DNA yield has been estimated by nanodrop (ThermoFisher) and quality on 2% agarose gel. RNA libraries were prepared using TruSeq RNA Stranded mRNA. Illumina NovaSeq 6000 Experimental Factor: organism Piptadenia stipulacea ERX9332086 E-MTAB-11756:Piptadenia_stipulacea_102797_2_p ERP137928 PRJEB53148 Transcriptome sequencing of divergent Fabales ERS12080461 SAMEA14456175 Piptadenia_stipulacea_102797_2_p RNA-Seq TRANSCRIPTOMIC Oligo-dT The seeds were obtained from U.S. National Plant Germplasm System Seeds were germinated in Petri dishes in the temperature optimal for each species and transferred to the pots in the cultivation room (photoperiod: 16h/8h, temperature: 22°C, humidity: 70 – 80%). No symbiotic bacteria was added to the soil. RNA isolation was carried out according to the manufacturer's protocol of Spectrum Plant Total RNA Kit (Sigma Aldrich) with minor modifications. Optimization of the protocol included decreasing the amount of ground tissue used to extraction (50 mg instead of proposed 100 mg). The incubation was performed for 5 min at 56°C. Moreover, 750 μL of the binding solution (instead of the proposed 500μL) was added. The DNase digestion was incorporated with the use of the protocol On-Column DNase I Digestion Set (Sigma Aldrich). RNA concentration and quality was measured with the use of Experion Automated Electrophoresis System, RNA StdSens Analysis Kit (Bio-Rad).High-quality DNA was isolated with the use of DNeasy Plant Pro Kit (Qiagen) with no changes to the protocol made. DNA yield has been estimated by nanodrop (ThermoFisher) and quality on 2% agarose gel. RNA libraries were prepared using TruSeq RNA Stranded mRNA. Illumina NovaSeq 6000 Experimental Factor: organism Piptadenia stipulacea ERX9332087 E-MTAB-11756:Piptadenia_stipulacea_102797_3_p ERP137928 PRJEB53148 Transcriptome sequencing of divergent Fabales ERS12080462 SAMEA14456176 Piptadenia_stipulacea_102797_3_p RNA-Seq TRANSCRIPTOMIC Oligo-dT The seeds were obtained from U.S. National Plant Germplasm System Seeds were germinated in Petri dishes in the temperature optimal for each species and transferred to the pots in the cultivation room (photoperiod: 16h/8h, temperature: 22°C, humidity: 70 – 80%). No symbiotic bacteria was added to the soil. RNA isolation was carried out according to the manufacturer's protocol of Spectrum Plant Total RNA Kit (Sigma Aldrich) with minor modifications. Optimization of the protocol included decreasing the amount of ground tissue used to extraction (50 mg instead of proposed 100 mg). The incubation was performed for 5 min at 56°C. Moreover, 750 μL of the binding solution (instead of the proposed 500μL) was added. The DNase digestion was incorporated with the use of the protocol On-Column DNase I Digestion Set (Sigma Aldrich). RNA concentration and quality was measured with the use of Experion Automated Electrophoresis System, RNA StdSens Analysis Kit (Bio-Rad).High-quality DNA was isolated with the use of DNeasy Plant Pro Kit (Qiagen) with no changes to the protocol made. DNA yield has been estimated by nanodrop (ThermoFisher) and quality on 2% agarose gel. RNA libraries were prepared using TruSeq RNA Stranded mRNA. Illumina NovaSeq 6000 Experimental Factor: organism Piptadenia stipulacea ERX9332088 E-MTAB-11756:Senna_obtusifolia_92737_p ERP137928 PRJEB53148 Transcriptome sequencing of divergent Fabales ERS12080463 SAMEA14456177 Senna_obtusifolia_92737_p RNA-Seq TRANSCRIPTOMIC Oligo-dT The seeds were obtained from U.S. National Plant Germplasm System Seeds were germinated in Petri dishes in the temperature optimal for each species and transferred to the pots in the cultivation room (photoperiod: 16h/8h, temperature: 22°C, humidity: 70 – 80%). No symbiotic bacteria was added to the soil. RNA isolation was carried out according to the manufacturer's protocol of Spectrum Plant Total RNA Kit (Sigma Aldrich) with minor modifications. Optimization of the protocol included decreasing the amount of ground tissue used to extraction (50 mg instead of proposed 100 mg). The incubation was performed for 5 min at 56°C. Moreover, 750 μL of the binding solution (instead of the proposed 500μL) was added. The DNase digestion was incorporated with the use of the protocol On-Column DNase I Digestion Set (Sigma Aldrich). RNA concentration and quality was measured with the use of Experion Automated Electrophoresis System, RNA StdSens Analysis Kit (Bio-Rad).High-quality DNA was isolated with the use of DNeasy Plant Pro Kit (Qiagen) with no changes to the protocol made. DNA yield has been estimated by nanodrop (ThermoFisher) and quality on 2% agarose gel. RNA libraries were prepared using TruSeq RNA Stranded mRNA. Illumina NovaSeq 6000 Experimental Factor: organism Senna obtusifolia ERX9332089 E-MTAB-11756:Senna_obtusifolia_92737_2_p ERP137928 PRJEB53148 Transcriptome sequencing of divergent Fabales ERS12080464 SAMEA14456178 Senna_obtusifolia_92737_2_p RNA-Seq TRANSCRIPTOMIC Oligo-dT The seeds were obtained from U.S. National Plant Germplasm System Seeds were germinated in Petri dishes in the temperature optimal for each species and transferred to the pots in the cultivation room (photoperiod: 16h/8h, temperature: 22°C, humidity: 70 – 80%). No symbiotic bacteria was added to the soil. RNA isolation was carried out according to the manufacturer's protocol of Spectrum Plant Total RNA Kit (Sigma Aldrich) with minor modifications. Optimization of the protocol included decreasing the amount of ground tissue used to extraction (50 mg instead of proposed 100 mg). The incubation was performed for 5 min at 56°C. Moreover, 750 μL of the binding solution (instead of the proposed 500μL) was added. The DNase digestion was incorporated with the use of the protocol On-Column DNase I Digestion Set (Sigma Aldrich). RNA concentration and quality was measured with the use of Experion Automated Electrophoresis System, RNA StdSens Analysis Kit (Bio-Rad).High-quality DNA was isolated with the use of DNeasy Plant Pro Kit (Qiagen) with no changes to the protocol made. DNA yield has been estimated by nanodrop (ThermoFisher) and quality on 2% agarose gel. RNA libraries were prepared using TruSeq RNA Stranded mRNA. Illumina NovaSeq 6000 Experimental Factor: organism Senna obtusifolia ERX9332090 E-MTAB-11756:Senna_obtusifolia_92737_3_p ERP137928 PRJEB53148 Transcriptome sequencing of divergent Fabales ERS12080465 SAMEA14456179 Senna_obtusifolia_92737_3_p RNA-Seq TRANSCRIPTOMIC Oligo-dT The seeds were obtained from U.S. National Plant Germplasm System Seeds were germinated in Petri dishes in the temperature optimal for each species and transferred to the pots in the cultivation room (photoperiod: 16h/8h, temperature: 22°C, humidity: 70 – 80%). No symbiotic bacteria was added to the soil. RNA isolation was carried out according to the manufacturer's protocol of Spectrum Plant Total RNA Kit (Sigma Aldrich) with minor modifications. Optimization of the protocol included decreasing the amount of ground tissue used to extraction (50 mg instead of proposed 100 mg). The incubation was performed for 5 min at 56°C. Moreover, 750 μL of the binding solution (instead of the proposed 500μL) was added. The DNase digestion was incorporated with the use of the protocol On-Column DNase I Digestion Set (Sigma Aldrich). RNA concentration and quality was measured with the use of Experion Automated Electrophoresis System, RNA StdSens Analysis Kit (Bio-Rad).High-quality DNA was isolated with the use of DNeasy Plant Pro Kit (Qiagen) with no changes to the protocol made. DNA yield has been estimated by nanodrop (ThermoFisher) and quality on 2% agarose gel. RNA libraries were prepared using TruSeq RNA Stranded mRNA. Illumina NovaSeq 6000 Experimental Factor: organism Senna obtusifolia