ERX9334777 E-MTAB-11776:Sample 1_p ERP137937 PRJEB53153 RNA-seq of depdc5-/-, tsc2-/-, depdc5-/- x tsc2-/- and wildtype zebrafish larvae ERS12088349 SAMEA14460872 Sample 1_p RNA-Seq TRANSCRIPTOMIC PolyA 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent (Invitrogen), followed by phenol-chlorophorm extraction and isopropanol precipitation. After ethanol washes, the RNA pellet was air-dried, dissolved in nuclease-free water (Fermentas). Illumina TruSeq Stranded mRNA-Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines. Illumina NovaSeq 6000 Experimental Factor: genotype depdc5-/- x tsc2-/- ERX9334778 E-MTAB-11776:Sample 10_p ERP137937 PRJEB53153 RNA-seq of depdc5-/-, tsc2-/-, depdc5-/- x tsc2-/- and wildtype zebrafish larvae ERS12088350 SAMEA14460873 Sample 10_p RNA-Seq TRANSCRIPTOMIC PolyA 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent (Invitrogen), followed by phenol-chlorophorm extraction and isopropanol precipitation. After ethanol washes, the RNA pellet was air-dried, dissolved in nuclease-free water (Fermentas). Illumina TruSeq Stranded mRNA-Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines. Illumina NovaSeq 6000 Experimental Factor: genotype tsc2-/- ERX9334779 E-MTAB-11776:Sample 11_p ERP137937 PRJEB53153 RNA-seq of depdc5-/-, tsc2-/-, depdc5-/- x tsc2-/- and wildtype zebrafish larvae ERS12088351 SAMEA14460874 Sample 11_p RNA-Seq TRANSCRIPTOMIC PolyA 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent (Invitrogen), followed by phenol-chlorophorm extraction and isopropanol precipitation. After ethanol washes, the RNA pellet was air-dried, dissolved in nuclease-free water (Fermentas). Illumina TruSeq Stranded mRNA-Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines. Illumina NovaSeq 6000 Experimental Factor: genotype tsc2-/- ERX9334780 E-MTAB-11776:Sample 12_p ERP137937 PRJEB53153 RNA-seq of depdc5-/-, tsc2-/-, depdc5-/- x tsc2-/- and wildtype zebrafish larvae ERS12088352 SAMEA14460875 Sample 12_p RNA-Seq TRANSCRIPTOMIC PolyA 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent (Invitrogen), followed by phenol-chlorophorm extraction and isopropanol precipitation. After ethanol washes, the RNA pellet was air-dried, dissolved in nuclease-free water (Fermentas). Illumina TruSeq Stranded mRNA-Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines. Illumina NovaSeq 6000 Experimental Factor: genotype tsc2-/- ERX9334781 E-MTAB-11776:Sample 13_p ERP137937 PRJEB53153 RNA-seq of depdc5-/-, tsc2-/-, depdc5-/- x tsc2-/- and wildtype zebrafish larvae ERS12088353 SAMEA14460876 Sample 13_p RNA-Seq TRANSCRIPTOMIC PolyA 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent (Invitrogen), followed by phenol-chlorophorm extraction and isopropanol precipitation. After ethanol washes, the RNA pellet was air-dried, dissolved in nuclease-free water (Fermentas). Illumina TruSeq Stranded mRNA-Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines. Illumina NovaSeq 6000 Experimental Factor: genotype wild type genotype ERX9334782 E-MTAB-11776:Sample 14_p ERP137937 PRJEB53153 RNA-seq of depdc5-/-, tsc2-/-, depdc5-/- x tsc2-/- and wildtype zebrafish larvae ERS12088354 SAMEA14460877 Sample 14_p RNA-Seq TRANSCRIPTOMIC PolyA 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent (Invitrogen), followed by phenol-chlorophorm extraction and isopropanol precipitation. After ethanol washes, the RNA pellet was air-dried, dissolved in nuclease-free water (Fermentas). Illumina TruSeq Stranded mRNA-Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines. Illumina NovaSeq 6000 Experimental Factor: genotype wild type genotype ERX9334783 E-MTAB-11776:Sample 15_p ERP137937 PRJEB53153 RNA-seq of depdc5-/-, tsc2-/-, depdc5-/- x tsc2-/- and wildtype zebrafish larvae ERS12088355 SAMEA14460878 Sample 15_p RNA-Seq TRANSCRIPTOMIC PolyA 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent (Invitrogen), followed by phenol-chlorophorm extraction and isopropanol precipitation. After ethanol washes, the RNA pellet was air-dried, dissolved in nuclease-free water (Fermentas). Illumina TruSeq Stranded mRNA-Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines. Illumina NovaSeq 6000 Experimental Factor: genotype wild type genotype ERX9334784 E-MTAB-11776:Sample 16_p ERP137937 PRJEB53153 RNA-seq of depdc5-/-, tsc2-/-, depdc5-/- x tsc2-/- and wildtype zebrafish larvae ERS12088356 SAMEA14460879 Sample 16_p RNA-Seq TRANSCRIPTOMIC PolyA 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent (Invitrogen), followed by phenol-chlorophorm extraction and isopropanol precipitation. After ethanol washes, the RNA pellet was air-dried, dissolved in nuclease-free water (Fermentas). Illumina TruSeq Stranded mRNA-Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines. Illumina NovaSeq 6000 Experimental Factor: genotype wild type genotype ERX9334785 E-MTAB-11776:Sample 2_p ERP137937 PRJEB53153 RNA-seq of depdc5-/-, tsc2-/-, depdc5-/- x tsc2-/- and wildtype zebrafish larvae ERS12088357 SAMEA14460880 Sample 2_p RNA-Seq TRANSCRIPTOMIC PolyA 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent (Invitrogen), followed by phenol-chlorophorm extraction and isopropanol precipitation. After ethanol washes, the RNA pellet was air-dried, dissolved in nuclease-free water (Fermentas). Illumina TruSeq Stranded mRNA-Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines. Illumina NovaSeq 6000 Experimental Factor: genotype depdc5-/- x tsc2-/- ERX9334786 E-MTAB-11776:Sample 3_p ERP137937 PRJEB53153 RNA-seq of depdc5-/-, tsc2-/-, depdc5-/- x tsc2-/- and wildtype zebrafish larvae ERS12088358 SAMEA14460881 Sample 3_p RNA-Seq TRANSCRIPTOMIC PolyA 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent (Invitrogen), followed by phenol-chlorophorm extraction and isopropanol precipitation. After ethanol washes, the RNA pellet was air-dried, dissolved in nuclease-free water (Fermentas). Illumina TruSeq Stranded mRNA-Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines. Illumina NovaSeq 6000 Experimental Factor: genotype depdc5-/- x tsc2-/- ERX9334787 E-MTAB-11776:Sample 4_p ERP137937 PRJEB53153 RNA-seq of depdc5-/-, tsc2-/-, depdc5-/- x tsc2-/- and wildtype zebrafish larvae ERS12088359 SAMEA14460882 Sample 4_p RNA-Seq TRANSCRIPTOMIC PolyA 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent (Invitrogen), followed by phenol-chlorophorm extraction and isopropanol precipitation. After ethanol washes, the RNA pellet was air-dried, dissolved in nuclease-free water (Fermentas). Illumina TruSeq Stranded mRNA-Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines. Illumina NovaSeq 6000 Experimental Factor: genotype depdc5-/- x tsc2-/- ERX9334788 E-MTAB-11776:Sample 5_p ERP137937 PRJEB53153 RNA-seq of depdc5-/-, tsc2-/-, depdc5-/- x tsc2-/- and wildtype zebrafish larvae ERS12088360 SAMEA14460883 Sample 5_p RNA-Seq TRANSCRIPTOMIC PolyA 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent (Invitrogen), followed by phenol-chlorophorm extraction and isopropanol precipitation. After ethanol washes, the RNA pellet was air-dried, dissolved in nuclease-free water (Fermentas). Illumina TruSeq Stranded mRNA-Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines. Illumina NovaSeq 6000 Experimental Factor: genotype depdc5-/- ERX9334789 E-MTAB-11776:Sample 6_p ERP137937 PRJEB53153 RNA-seq of depdc5-/-, tsc2-/-, depdc5-/- x tsc2-/- and wildtype zebrafish larvae ERS12088361 SAMEA14460884 Sample 6_p RNA-Seq TRANSCRIPTOMIC PolyA 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent (Invitrogen), followed by phenol-chlorophorm extraction and isopropanol precipitation. After ethanol washes, the RNA pellet was air-dried, dissolved in nuclease-free water (Fermentas). Illumina TruSeq Stranded mRNA-Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines. Illumina NovaSeq 6000 Experimental Factor: genotype depdc5-/- ERX9334790 E-MTAB-11776:Sample 7_p ERP137937 PRJEB53153 RNA-seq of depdc5-/-, tsc2-/-, depdc5-/- x tsc2-/- and wildtype zebrafish larvae ERS12088362 SAMEA14460885 Sample 7_p RNA-Seq TRANSCRIPTOMIC PolyA 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent (Invitrogen), followed by phenol-chlorophorm extraction and isopropanol precipitation. After ethanol washes, the RNA pellet was air-dried, dissolved in nuclease-free water (Fermentas). Illumina TruSeq Stranded mRNA-Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines. Illumina NovaSeq 6000 Experimental Factor: genotype depdc5-/- ERX9334791 E-MTAB-11776:Sample 8_p ERP137937 PRJEB53153 RNA-seq of depdc5-/-, tsc2-/-, depdc5-/- x tsc2-/- and wildtype zebrafish larvae ERS12088363 SAMEA14460886 Sample 8_p RNA-Seq TRANSCRIPTOMIC PolyA 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent (Invitrogen), followed by phenol-chlorophorm extraction and isopropanol precipitation. After ethanol washes, the RNA pellet was air-dried, dissolved in nuclease-free water (Fermentas). Illumina TruSeq Stranded mRNA-Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines. Illumina NovaSeq 6000 Experimental Factor: genotype depdc5-/- ERX9334792 E-MTAB-11776:Sample 9_p ERP137937 PRJEB53153 RNA-seq of depdc5-/-, tsc2-/-, depdc5-/- x tsc2-/- and wildtype zebrafish larvae ERS12088364 SAMEA14460887 Sample 9_p RNA-Seq TRANSCRIPTOMIC PolyA 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent (Invitrogen), followed by phenol-chlorophorm extraction and isopropanol precipitation. After ethanol washes, the RNA pellet was air-dried, dissolved in nuclease-free water (Fermentas). Illumina TruSeq Stranded mRNA-Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines. Illumina NovaSeq 6000 Experimental Factor: genotype tsc2-/-