<?xml version="1.0" encoding="UTF-8"?>
<STUDY_SET xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
  <STUDY accession="ERP109211" alias="E-MTAB-6856" broker_name="ArrayExpress" center_name="The Institute of Cancer Research">
    <IDENTIFIERS>
      <PRIMARY_ID>ERP109211</PRIMARY_ID>
      <EXTERNAL_ID namespace="BioProject">PRJEB27161</EXTERNAL_ID>
      <SUBMITTER_ID namespace="The Institute of Cancer Research">E-MTAB-6856</SUBMITTER_ID>
    </IDENTIFIERS>
    <DESCRIPTOR>
      <STUDY_TITLE>RNA-seq of embryonic mammary progenitor cell lines in compari (part A)</STUDY_TITLE>
      <STUDY_TYPE existing_study_type="Transcriptome Analysis"/>
      <STUDY_ABSTRACT>RNA-seq of embryonic mammary progenitor cell lines. Embryonic mammary progenitor cell (eMPC) clones were derived from E12.5-stage mammary organs. After microdissection, mammary primordial 3 was cultured on thick basement membrane extract for 4 weeks. After enzymatic dissociation eMPC swere plated and expanded in 2D culture to obtain a pool of eMPCs. eMPC cells were subject to single cell sorting using FACS. Clones were expanded as single-cell derived clones (eMPC1, 2, etc.) to create the eighteen single cell-derived clones described in this study.</STUDY_ABSTRACT>
      <STUDY_DESCRIPTION>RNA-seq of embryonic mammary progenitor cell lines. Embryonic mammary progenitor cell (eMPC) clones were derived from E12.5-stage mammary organs. After microdissection, mammary primordial 3 was cultured on thick basement membrane extract for 4 weeks. After enzymatic dissociation eMPC swere plated and expanded in 2D culture to obtain a pool of eMPCs. eMPC cells were subject to single cell sorting using FACS. Clones were expanded as single-cell derived clones (eMPC1, 2, etc.) to create the eighteen single cell-derived clones described in this study.</STUDY_DESCRIPTION>
    </DESCRIPTOR>
    <STUDY_LINKS>
      <STUDY_LINK>
        <URL_LINK>
          <LABEL>E-MTAB-6856 in ArrayExpress</LABEL>
          <URL>http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-6856</URL>
        </URL_LINK>
      </STUDY_LINK>
    </STUDY_LINKS>
    <STUDY_ATTRIBUTES>
      <STUDY_ATTRIBUTE>
        <TAG>ENA-FIRST-PUBLIC</TAG>
        <VALUE>2018-11-30</VALUE>
      </STUDY_ATTRIBUTE>
      <STUDY_ATTRIBUTE>
        <TAG>ENA-LAST-UPDATE</TAG>
        <VALUE>2018-06-06</VALUE>
      </STUDY_ATTRIBUTE>
    </STUDY_ATTRIBUTES>
  </STUDY>
</STUDY_SET>
