ERX2636871 E-MTAB-6870:HEP3B_2D_BR1_RNA_p ERP109267 PRJEB27210 RNA-seq analysis of several in vitro models of hepatocyte function: Project 4 of Open Targets - Epigenomes of Cell Lines ERS2537959 SAMEA4717812 HEP3B_2D_BR1_RNA_p RNA-Seq TRANSCRIPTOMIC cDNA For primary cultures, cells were obtained from three independent donors and cultured in parallel in both conditions. Cell lines were grown using standard culture methods and harvested sub-confluence in exponential growth phase. Qiagen RNeasy plus mini kit Total RNA from each sample was used as input for the Illumina TruSeq Stranded messenger RNA LT Sample Prep Kit (Illumina) and sequencing libraries were created according to the manufacturer's protocol. Briefly, poly-A containing mRNA molecules were purified using poly-T oligo-attached magnetic beads. Following purification, the mRNA was fragmented and copied into first strand complementary DNA using random primers and reverse transcriptase. Second strand cDNA synthesis was then done using DNA polymerase I, RNase H and substituting dUTP for dTTP. The cDNA was ligated to adapters and enriched with PCR to create the final cDNA library. The library was pooled and sequenced 150 0 F Application Read Forward 1 1 R Application Read Reverse 76 Illumina HiSeq 2500 Experimental Factor: cell line Hep3B Experimental Factor: growth condition 2D ERX2636872 E-MTAB-6870:HEP3B_2D_BR2_RNA_p ERP109267 PRJEB27210 RNA-seq analysis of several in vitro models of hepatocyte function: Project 4 of Open Targets - Epigenomes of Cell Lines ERS2537960 SAMEA4717813 HEP3B_2D_BR2_RNA_p RNA-Seq TRANSCRIPTOMIC cDNA For primary cultures, cells were obtained from three independent donors and cultured in parallel in both conditions. Cell lines were grown using standard culture methods and harvested sub-confluence in exponential growth phase. Qiagen RNeasy plus mini kit Total RNA from each sample was used as input for the Illumina TruSeq Stranded messenger RNA LT Sample Prep Kit (Illumina) and sequencing libraries were created according to the manufacturer's protocol. Briefly, poly-A containing mRNA molecules were purified using poly-T oligo-attached magnetic beads. Following purification, the mRNA was fragmented and copied into first strand complementary DNA using random primers and reverse transcriptase. Second strand cDNA synthesis was then done using DNA polymerase I, RNase H and substituting dUTP for dTTP. The cDNA was ligated to adapters and enriched with PCR to create the final cDNA library. The library was pooled and sequenced 150 0 F Application Read Forward 1 1 R Application Read Reverse 76 Illumina HiSeq 2500 Experimental Factor: cell line Hep3B Experimental Factor: growth condition 2D ERX2636873 E-MTAB-6870:HEP3B_3D_BR1_RNA_p ERP109267 PRJEB27210 RNA-seq analysis of several in vitro models of hepatocyte function: Project 4 of Open Targets - Epigenomes of Cell Lines ERS2537961 SAMEA4717814 HEP3B_3D_BR1_RNA_p RNA-Seq TRANSCRIPTOMIC cDNA For primary cultures, cells were obtained from three independent donors and cultured in parallel in both conditions. Cells were seeded at 1500 cells per well in ultra-low adherence multi-well plates to facilitate cellular aggregation and spheroid formation. Cell line spheroids aggregated rapidly and were harvested 4-5 days post seeding. Qiagen RNeasy plus mini kit Total RNA from each sample was used as input for the Illumina TruSeq Stranded messenger RNA LT Sample Prep Kit (Illumina) and sequencing libraries were created according to the manufacturer's protocol. Briefly, poly-A containing mRNA molecules were purified using poly-T oligo-attached magnetic beads. Following purification, the mRNA was fragmented and copied into first strand complementary DNA using random primers and reverse transcriptase. Second strand cDNA synthesis was then done using DNA polymerase I, RNase H and substituting dUTP for dTTP. The cDNA was ligated to adapters and enriched with PCR to create the final cDNA library. The library was pooled and sequenced 150 0 F Application Read Forward 1 1 R Application Read Reverse 76 Illumina HiSeq 2500 Experimental Factor: cell line Hep3B Experimental Factor: growth condition 3D ERX2636874 E-MTAB-6870:HEP3B_3D_BR2_RNA_p ERP109267 PRJEB27210 RNA-seq analysis of several in vitro models of hepatocyte function: Project 4 of Open Targets - Epigenomes of Cell Lines ERS2537962 SAMEA4717815 HEP3B_3D_BR2_RNA_p RNA-Seq TRANSCRIPTOMIC cDNA For primary cultures, cells were obtained from three independent donors and cultured in parallel in both conditions. Cells were seeded at 1500 cells per well in ultra-low adherence multi-well plates to facilitate cellular aggregation and spheroid formation. Cell line spheroids aggregated rapidly and were harvested 4-5 days post seeding. Qiagen RNeasy plus mini kit Total RNA from each sample was used as input for the Illumina TruSeq Stranded messenger RNA LT Sample Prep Kit (Illumina) and sequencing libraries were created according to the manufacturer's protocol. Briefly, poly-A containing mRNA molecules were purified using poly-T oligo-attached magnetic beads. Following purification, the mRNA was fragmented and copied into first strand complementary DNA using random primers and reverse transcriptase. Second strand cDNA synthesis was then done using DNA polymerase I, RNase H and substituting dUTP for dTTP. The cDNA was ligated to adapters and enriched with PCR to create the final cDNA library. The library was pooled and sequenced 150 0 F Application Read Forward 1 1 R Application Read Reverse 76 Illumina HiSeq 2500 Experimental Factor: cell line Hep3B Experimental Factor: growth condition 3D ERX2636875 E-MTAB-6870:HEPG2_2D_BR1_RNA_p ERP109267 PRJEB27210 RNA-seq analysis of several in vitro models of hepatocyte function: Project 4 of Open Targets - Epigenomes of Cell Lines ERS2537963 SAMEA4717816 HEPG2_2D_BR1_RNA_p RNA-Seq TRANSCRIPTOMIC cDNA For primary cultures, cells were obtained from three independent donors and cultured in parallel in both conditions. Cell lines were grown using standard culture methods and harvested sub-confluence in exponential growth phase. Qiagen RNeasy plus mini kit Total RNA from each sample was used as input for the Illumina TruSeq Stranded messenger RNA LT Sample Prep Kit (Illumina) and sequencing libraries were created according to the manufacturer's protocol. Briefly, poly-A containing mRNA molecules were purified using poly-T oligo-attached magnetic beads. Following purification, the mRNA was fragmented and copied into first strand complementary DNA using random primers and reverse transcriptase. Second strand cDNA synthesis was then done using DNA polymerase I, RNase H and substituting dUTP for dTTP. The cDNA was ligated to adapters and enriched with PCR to create the final cDNA library. The library was pooled and sequenced 150 0 F Application Read Forward 1 1 R Application Read Reverse 76 Illumina HiSeq 2500 Experimental Factor: cell line HepG2 Experimental Factor: growth condition 2D ERX2636876 E-MTAB-6870:HEPG2_2D_BR2_RNA_p ERP109267 PRJEB27210 RNA-seq analysis of several in vitro models of hepatocyte function: Project 4 of Open Targets - Epigenomes of Cell Lines ERS2537964 SAMEA4717817 HEPG2_2D_BR2_RNA_p RNA-Seq TRANSCRIPTOMIC cDNA For primary cultures, cells were obtained from three independent donors and cultured in parallel in both conditions. Cell lines were grown using standard culture methods and harvested sub-confluence in exponential growth phase. Qiagen RNeasy plus mini kit Total RNA from each sample was used as input for the Illumina TruSeq Stranded messenger RNA LT Sample Prep Kit (Illumina) and sequencing libraries were created according to the manufacturer's protocol. Briefly, poly-A containing mRNA molecules were purified using poly-T oligo-attached magnetic beads. Following purification, the mRNA was fragmented and copied into first strand complementary DNA using random primers and reverse transcriptase. Second strand cDNA synthesis was then done using DNA polymerase I, RNase H and substituting dUTP for dTTP. The cDNA was ligated to adapters and enriched with PCR to create the final cDNA library. The library was pooled and sequenced 150 0 F Application Read Forward 1 1 R Application Read Reverse 76 Illumina HiSeq 2500 Experimental Factor: cell line HepG2 Experimental Factor: growth condition 2D ERX2636877 E-MTAB-6870:HEPG2_3D_BR1_RNA_p ERP109267 PRJEB27210 RNA-seq analysis of several in vitro models of hepatocyte function: Project 4 of Open Targets - Epigenomes of Cell Lines ERS2537965 SAMEA4717818 HEPG2_3D_BR1_RNA_p RNA-Seq TRANSCRIPTOMIC cDNA For primary cultures, cells were obtained from three independent donors and cultured in parallel in both conditions. Cells were seeded at 1500 cells per well in ultra-low adherence multi-well plates to facilitate cellular aggregation and spheroid formation. Cell line spheroids aggregated rapidly and were harvested 4-5 days post seeding. Qiagen RNeasy plus mini kit Total RNA from each sample was used as input for the Illumina TruSeq Stranded messenger RNA LT Sample Prep Kit (Illumina) and sequencing libraries were created according to the manufacturer's protocol. Briefly, poly-A containing mRNA molecules were purified using poly-T oligo-attached magnetic beads. Following purification, the mRNA was fragmented and copied into first strand complementary DNA using random primers and reverse transcriptase. Second strand cDNA synthesis was then done using DNA polymerase I, RNase H and substituting dUTP for dTTP. The cDNA was ligated to adapters and enriched with PCR to create the final cDNA library. The library was pooled and sequenced 150 0 F Application Read Forward 1 1 R Application Read Reverse 76 Illumina HiSeq 2500 Experimental Factor: cell line HepG2 Experimental Factor: growth condition 3D ERX2636878 E-MTAB-6870:HEPG2_3D_BR2_RNA_p ERP109267 PRJEB27210 RNA-seq analysis of several in vitro models of hepatocyte function: Project 4 of Open Targets - Epigenomes of Cell Lines ERS2537966 SAMEA4717819 HEPG2_3D_BR2_RNA_p RNA-Seq TRANSCRIPTOMIC cDNA For primary cultures, cells were obtained from three independent donors and cultured in parallel in both conditions. Cells were seeded at 1500 cells per well in ultra-low adherence multi-well plates to facilitate cellular aggregation and spheroid formation. Cell line spheroids aggregated rapidly and were harvested 4-5 days post seeding. Qiagen RNeasy plus mini kit Total RNA from each sample was used as input for the Illumina TruSeq Stranded messenger RNA LT Sample Prep Kit (Illumina) and sequencing libraries were created according to the manufacturer's protocol. Briefly, poly-A containing mRNA molecules were purified using poly-T oligo-attached magnetic beads. Following purification, the mRNA was fragmented and copied into first strand complementary DNA using random primers and reverse transcriptase. Second strand cDNA synthesis was then done using DNA polymerase I, RNase H and substituting dUTP for dTTP. The cDNA was ligated to adapters and enriched with PCR to create the final cDNA library. The library was pooled and sequenced 150 0 F Application Read Forward 1 1 R Application Read Reverse 76 Illumina HiSeq 2500 Experimental Factor: cell line HepG2 Experimental Factor: growth condition 3D ERX2636879 E-MTAB-6870:HUH7_2D_BR1_RNA_p ERP109267 PRJEB27210 RNA-seq analysis of several in vitro models of hepatocyte function: Project 4 of Open Targets - Epigenomes of Cell Lines ERS2537967 SAMEA4717820 HUH7_2D_BR1_RNA_p RNA-Seq TRANSCRIPTOMIC cDNA For primary cultures, cells were obtained from three independent donors and cultured in parallel in both conditions. Cell lines were grown using standard culture methods and harvested sub-confluence in exponential growth phase. Qiagen RNeasy plus mini kit Total RNA from each sample was used as input for the Illumina TruSeq Stranded messenger RNA LT Sample Prep Kit (Illumina) and sequencing libraries were created according to the manufacturer's protocol. Briefly, poly-A containing mRNA molecules were purified using poly-T oligo-attached magnetic beads. Following purification, the mRNA was fragmented and copied into first strand complementary DNA using random primers and reverse transcriptase. Second strand cDNA synthesis was then done using DNA polymerase I, RNase H and substituting dUTP for dTTP. The cDNA was ligated to adapters and enriched with PCR to create the final cDNA library. The library was pooled and sequenced 150 0 F Application Read Forward 1 1 R Application Read Reverse 76 Illumina HiSeq 2500 Experimental Factor: cell line HuH-7 Experimental Factor: growth condition 2D ERX2636880 E-MTAB-6870:HUH7_2D_BR2_RNA_p ERP109267 PRJEB27210 RNA-seq analysis of several in vitro models of hepatocyte function: Project 4 of Open Targets - Epigenomes of Cell Lines ERS2537968 SAMEA4717821 HUH7_2D_BR2_RNA_p RNA-Seq TRANSCRIPTOMIC cDNA For primary cultures, cells were obtained from three independent donors and cultured in parallel in both conditions. Cell lines were grown using standard culture methods and harvested sub-confluence in exponential growth phase. Qiagen RNeasy plus mini kit Total RNA from each sample was used as input for the Illumina TruSeq Stranded messenger RNA LT Sample Prep Kit (Illumina) and sequencing libraries were created according to the manufacturer's protocol. Briefly, poly-A containing mRNA molecules were purified using poly-T oligo-attached magnetic beads. Following purification, the mRNA was fragmented and copied into first strand complementary DNA using random primers and reverse transcriptase. Second strand cDNA synthesis was then done using DNA polymerase I, RNase H and substituting dUTP for dTTP. The cDNA was ligated to adapters and enriched with PCR to create the final cDNA library. The library was pooled and sequenced 150 0 F Application Read Forward 1 1 R Application Read Reverse 76 Illumina HiSeq 2500 Experimental Factor: cell line HuH-7 Experimental Factor: growth condition 2D ERX2636881 E-MTAB-6870:HUH7_3D_BR1_RNA_p ERP109267 PRJEB27210 RNA-seq analysis of several in vitro models of hepatocyte function: Project 4 of Open Targets - Epigenomes of Cell Lines ERS2537969 SAMEA4717822 HUH7_3D_BR1_RNA_p RNA-Seq TRANSCRIPTOMIC cDNA For primary cultures, cells were obtained from three independent donors and cultured in parallel in both conditions. Cells were seeded at 1500 cells per well in ultra-low adherence multi-well plates to facilitate cellular aggregation and spheroid formation. Cell line spheroids aggregated rapidly and were harvested 4-5 days post seeding. Qiagen RNeasy plus mini kit Total RNA from each sample was used as input for the Illumina TruSeq Stranded messenger RNA LT Sample Prep Kit (Illumina) and sequencing libraries were created according to the manufacturer's protocol. Briefly, poly-A containing mRNA molecules were purified using poly-T oligo-attached magnetic beads. Following purification, the mRNA was fragmented and copied into first strand complementary DNA using random primers and reverse transcriptase. Second strand cDNA synthesis was then done using DNA polymerase I, RNase H and substituting dUTP for dTTP. The cDNA was ligated to adapters and enriched with PCR to create the final cDNA library. The library was pooled and sequenced 150 0 F Application Read Forward 1 1 R Application Read Reverse 76 Illumina HiSeq 2500 Experimental Factor: cell line HuH-7 Experimental Factor: growth condition 3D ERX2636882 E-MTAB-6870:HUH7_3D_BR2_RNA_p ERP109267 PRJEB27210 RNA-seq analysis of several in vitro models of hepatocyte function: Project 4 of Open Targets - Epigenomes of Cell Lines ERS2537970 SAMEA4717823 HUH7_3D_BR2_RNA_p RNA-Seq TRANSCRIPTOMIC cDNA For primary cultures, cells were obtained from three independent donors and cultured in parallel in both conditions. Cells were seeded at 1500 cells per well in ultra-low adherence multi-well plates to facilitate cellular aggregation and spheroid formation. Cell line spheroids aggregated rapidly and were harvested 4-5 days post seeding. Qiagen RNeasy plus mini kit Total RNA from each sample was used as input for the Illumina TruSeq Stranded messenger RNA LT Sample Prep Kit (Illumina) and sequencing libraries were created according to the manufacturer's protocol. Briefly, poly-A containing mRNA molecules were purified using poly-T oligo-attached magnetic beads. Following purification, the mRNA was fragmented and copied into first strand complementary DNA using random primers and reverse transcriptase. Second strand cDNA synthesis was then done using DNA polymerase I, RNase H and substituting dUTP for dTTP. The cDNA was ligated to adapters and enriched with PCR to create the final cDNA library. The library was pooled and sequenced 150 0 F Application Read Forward 1 1 R Application Read Reverse 76 Illumina HiSeq 2500 Experimental Factor: cell line HuH-7 Experimental Factor: growth condition 3D ERX2636883 E-MTAB-6870:PHH_D1_2D_RNA_p ERP109267 PRJEB27210 RNA-seq analysis of several in vitro models of hepatocyte function: Project 4 of Open Targets - Epigenomes of Cell Lines ERS2537971 SAMEA4717824 PHH_D1_2D_RNA_p RNA-Seq TRANSCRIPTOMIC cDNA For primary cultures, cells were obtained from three independent donors and cultured in parallel in both conditions. Cells were grown in sandwich configuration between two layers of extracellular matrix (collagen coated plates and medium containing 0.25mg/ml Matrigel) and harvested at day 9 post seeding. Qiagen RNeasy plus mini kit Total RNA from each sample was used as input for the Illumina TruSeq Stranded messenger RNA LT Sample Prep Kit (Illumina) and sequencing libraries were created according to the manufacturer's protocol. Briefly, poly-A containing mRNA molecules were purified using poly-T oligo-attached magnetic beads. Following purification, the mRNA was fragmented and copied into first strand complementary DNA using random primers and reverse transcriptase. Second strand cDNA synthesis was then done using DNA polymerase I, RNase H and substituting dUTP for dTTP. The cDNA was ligated to adapters and enriched with PCR to create the final cDNA library. The library was pooled and sequenced 150 0 F Application Read Forward 1 1 R Application Read Reverse 76 Illumina HiSeq 2500 Experimental Factor: cell line primary cell line Experimental Factor: growth condition 2D ERX2636884 E-MTAB-6870:PHH_D2_2D_RNA_p ERP109267 PRJEB27210 RNA-seq analysis of several in vitro models of hepatocyte function: Project 4 of Open Targets - Epigenomes of Cell Lines ERS2537972 SAMEA4717825 PHH_D2_2D_RNA_p RNA-Seq TRANSCRIPTOMIC cDNA For primary cultures, cells were obtained from three independent donors and cultured in parallel in both conditions. Cells were grown in sandwich configuration between two layers of extracellular matrix (collagen coated plates and medium containing 0.25mg/ml Matrigel) and harvested at day 9 post seeding. Qiagen RNeasy plus mini kit Total RNA from each sample was used as input for the Illumina TruSeq Stranded messenger RNA LT Sample Prep Kit (Illumina) and sequencing libraries were created according to the manufacturer's protocol. Briefly, poly-A containing mRNA molecules were purified using poly-T oligo-attached magnetic beads. Following purification, the mRNA was fragmented and copied into first strand complementary DNA using random primers and reverse transcriptase. Second strand cDNA synthesis was then done using DNA polymerase I, RNase H and substituting dUTP for dTTP. The cDNA was ligated to adapters and enriched with PCR to create the final cDNA library. The library was pooled and sequenced 150 0 F Application Read Forward 1 1 R Application Read Reverse 76 Illumina HiSeq 2500 Experimental Factor: cell line primary cell line Experimental Factor: growth condition 2D ERX2636885 E-MTAB-6870:PHH_D3_2D_RNA_p ERP109267 PRJEB27210 RNA-seq analysis of several in vitro models of hepatocyte function: Project 4 of Open Targets - Epigenomes of Cell Lines ERS2537973 SAMEA4717826 PHH_D3_2D_RNA_p RNA-Seq TRANSCRIPTOMIC cDNA For primary cultures, cells were obtained from three independent donors and cultured in parallel in both conditions. Cells were grown in sandwich configuration between two layers of extracellular matrix (collagen coated plates and medium containing 0.25mg/ml Matrigel) and harvested at day 9 post seeding. Qiagen RNeasy plus mini kit Total RNA from each sample was used as input for the Illumina TruSeq Stranded messenger RNA LT Sample Prep Kit (Illumina) and sequencing libraries were created according to the manufacturer's protocol. Briefly, poly-A containing mRNA molecules were purified using poly-T oligo-attached magnetic beads. Following purification, the mRNA was fragmented and copied into first strand complementary DNA using random primers and reverse transcriptase. Second strand cDNA synthesis was then done using DNA polymerase I, RNase H and substituting dUTP for dTTP. The cDNA was ligated to adapters and enriched with PCR to create the final cDNA library. The library was pooled and sequenced 150 0 F Application Read Forward 1 1 R Application Read Reverse 76 Illumina HiSeq 2500 Experimental Factor: cell line primary cell line Experimental Factor: growth condition 2D ERX2636886 E-MTAB-6870:PHH_D1_3D_RNA_p ERP109267 PRJEB27210 RNA-seq analysis of several in vitro models of hepatocyte function: Project 4 of Open Targets - Epigenomes of Cell Lines ERS2537974 SAMEA4717827 PHH_D1_3D_RNA_p RNA-Seq TRANSCRIPTOMIC cDNA For primary cultures, cells were obtained from three independent donors and cultured in parallel in both conditions. Cells were seeded at 1500 cells per well in ultra-low adherence multi-well plates to facilitate cellular aggregation and spheroid formation. Primary cell spheroids formed more slowly and were harvested 14-17 days post seeding. Qiagen RNeasy plus mini kit Total RNA from each sample was used as input for the Illumina TruSeq Stranded messenger RNA LT Sample Prep Kit (Illumina) and sequencing libraries were created according to the manufacturer's protocol. Briefly, poly-A containing mRNA molecules were purified using poly-T oligo-attached magnetic beads. Following purification, the mRNA was fragmented and copied into first strand complementary DNA using random primers and reverse transcriptase. Second strand cDNA synthesis was then done using DNA polymerase I, RNase H and substituting dUTP for dTTP. The cDNA was ligated to adapters and enriched with PCR to create the final cDNA library. The library was pooled and sequenced 150 0 F Application Read Forward 1 1 R Application Read Reverse 76 Illumina HiSeq 2500 Experimental Factor: cell line primary cell line Experimental Factor: growth condition 3D ERX2636887 E-MTAB-6870:PHH_D2_3D_RNA_p ERP109267 PRJEB27210 RNA-seq analysis of several in vitro models of hepatocyte function: Project 4 of Open Targets - Epigenomes of Cell Lines ERS2537975 SAMEA4717828 PHH_D2_3D_RNA_p RNA-Seq TRANSCRIPTOMIC cDNA For primary cultures, cells were obtained from three independent donors and cultured in parallel in both conditions. Cells were seeded at 1500 cells per well in ultra-low adherence multi-well plates to facilitate cellular aggregation and spheroid formation. Primary cell spheroids formed more slowly and were harvested 14-17 days post seeding. Qiagen RNeasy plus mini kit Total RNA from each sample was used as input for the Illumina TruSeq Stranded messenger RNA LT Sample Prep Kit (Illumina) and sequencing libraries were created according to the manufacturer's protocol. Briefly, poly-A containing mRNA molecules were purified using poly-T oligo-attached magnetic beads. Following purification, the mRNA was fragmented and copied into first strand complementary DNA using random primers and reverse transcriptase. Second strand cDNA synthesis was then done using DNA polymerase I, RNase H and substituting dUTP for dTTP. The cDNA was ligated to adapters and enriched with PCR to create the final cDNA library. The library was pooled and sequenced 150 0 F Application Read Forward 1 1 R Application Read Reverse 76 Illumina HiSeq 2500 Experimental Factor: cell line primary cell line Experimental Factor: growth condition 3D ERX2636888 E-MTAB-6870:PHH_D3_3D_RNA_p ERP109267 PRJEB27210 RNA-seq analysis of several in vitro models of hepatocyte function: Project 4 of Open Targets - Epigenomes of Cell Lines ERS2537976 SAMEA4717829 PHH_D3_3D_RNA_p RNA-Seq TRANSCRIPTOMIC cDNA For primary cultures, cells were obtained from three independent donors and cultured in parallel in both conditions. Cells were seeded at 1500 cells per well in ultra-low adherence multi-well plates to facilitate cellular aggregation and spheroid formation. Primary cell spheroids formed more slowly and were harvested 14-17 days post seeding. Qiagen RNeasy plus mini kit Total RNA from each sample was used as input for the Illumina TruSeq Stranded messenger RNA LT Sample Prep Kit (Illumina) and sequencing libraries were created according to the manufacturer's protocol. Briefly, poly-A containing mRNA molecules were purified using poly-T oligo-attached magnetic beads. Following purification, the mRNA was fragmented and copied into first strand complementary DNA using random primers and reverse transcriptase. Second strand cDNA synthesis was then done using DNA polymerase I, RNase H and substituting dUTP for dTTP. The cDNA was ligated to adapters and enriched with PCR to create the final cDNA library. The library was pooled and sequenced 150 0 F Application Read Forward 1 1 R Application Read Reverse 76 Illumina HiSeq 2500 Experimental Factor: cell line primary cell line Experimental Factor: growth condition 3D