ERS2544895 SAMEA4724758 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:55Z ENA-LAST-UPDATE 2018-06-12T15:12:58Z External Id SAMEA4724758 INSDC center name UNSW INSDC first public 2018-06-22T17:01:55Z INSDC last update 2018-06-12T15:12:58Z INSDC status public Submitter Id E-MTAB-6386:Sample 13 age 33 broker name ArrayExpress cell type memory B cell common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD- CD27+ IgG+ sample name E-MTAB-6386:Sample 13 scientific_name Homo sapiens sex female single cell quality OK ERS2544896 SAMEA4724759 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:55Z ENA-LAST-UPDATE 2018-06-12T15:12:58Z External Id SAMEA4724759 INSDC center name UNSW INSDC first public 2018-06-22T17:01:55Z INSDC last update 2018-06-12T15:12:58Z INSDC status public Submitter Id E-MTAB-6386:Sample 14 age 33 broker name ArrayExpress cell type memory B cell common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD- CD27+ IgG+ sample name E-MTAB-6386:Sample 14 scientific_name Homo sapiens sex female single cell quality OK ERS2544897 SAMEA4724760 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:55Z ENA-LAST-UPDATE 2018-06-12T15:12:58Z External Id SAMEA4724760 INSDC center name UNSW INSDC first public 2018-06-22T17:01:55Z INSDC last update 2018-06-12T15:12:58Z INSDC status public Submitter Id E-MTAB-6386:Sample 15 age 33 broker name ArrayExpress cell type memory B cell common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD- CD27+ IgG+ sample name E-MTAB-6386:Sample 15 scientific_name Homo sapiens sex female single cell quality OK ERS2544898 SAMEA4724761 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:55Z ENA-LAST-UPDATE 2018-06-12T15:12:58Z External Id SAMEA4724761 INSDC center name UNSW INSDC first public 2018-06-22T17:01:55Z INSDC last update 2018-06-12T15:12:58Z INSDC status public Submitter Id E-MTAB-6386:Sample 16 age 33 broker name ArrayExpress cell type memory B cell common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD- CD27+ IgG+ sample name E-MTAB-6386:Sample 16 scientific_name Homo sapiens sex female single cell quality OK ERS2544899 SAMEA4724762 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:55Z ENA-LAST-UPDATE 2018-06-12T15:12:58Z External Id SAMEA4724762 INSDC center name UNSW INSDC first public 2018-06-22T17:01:55Z INSDC last update 2018-06-12T15:12:58Z INSDC status public Submitter Id E-MTAB-6386:Sample 17 age 33 broker name ArrayExpress cell type memory B cell common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD- CD27+ IgG+ sample name E-MTAB-6386:Sample 17 scientific_name Homo sapiens sex female single cell quality OK ERS2544900 SAMEA4724763 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:55Z ENA-LAST-UPDATE 2018-06-12T15:12:58Z External Id SAMEA4724763 INSDC center name UNSW INSDC first public 2018-06-22T17:01:55Z INSDC last update 2018-06-12T15:12:58Z INSDC status public Submitter Id E-MTAB-6386:Sample 18 age 33 broker name ArrayExpress cell type memory B cell common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD- CD27+ IgG+ sample name E-MTAB-6386:Sample 18 scientific_name Homo sapiens sex female single cell quality OK ERS2544901 SAMEA4724764 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:55Z ENA-LAST-UPDATE 2018-06-12T15:12:58Z External Id SAMEA4724764 INSDC center name UNSW INSDC first public 2018-06-22T17:01:55Z INSDC last update 2018-06-12T15:12:58Z INSDC status public Submitter Id E-MTAB-6386:Sample 19 age 33 broker name ArrayExpress cell type memory B cell common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD- CD27+ IgG+ sample name E-MTAB-6386:Sample 19 scientific_name Homo sapiens sex female single cell quality OK ERS2544902 SAMEA4724765 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:55Z ENA-LAST-UPDATE 2018-06-12T15:12:59Z External Id SAMEA4724765 INSDC center name UNSW INSDC first public 2018-06-22T17:01:55Z INSDC last update 2018-06-12T15:12:59Z INSDC status public Submitter Id E-MTAB-6386:Sample 20 age 33 broker name ArrayExpress cell type memory B cell common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD- CD27+ IgG+ sample name E-MTAB-6386:Sample 20 scientific_name Homo sapiens sex female single cell quality OK ERS2544903 SAMEA4724766 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:55Z ENA-LAST-UPDATE 2018-06-12T15:12:59Z External Id SAMEA4724766 INSDC center name UNSW INSDC first public 2018-06-22T17:01:55Z INSDC last update 2018-06-12T15:12:59Z INSDC status public Submitter Id E-MTAB-6386:Sample 21 age 33 broker name ArrayExpress cell type memory B cell common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD- CD27+ IgG+ sample name E-MTAB-6386:Sample 21 scientific_name Homo sapiens sex female single cell quality OK ERS2544904 SAMEA4724767 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:55Z ENA-LAST-UPDATE 2018-06-12T15:12:59Z External Id SAMEA4724767 INSDC center name UNSW INSDC first public 2018-06-22T17:01:55Z INSDC last update 2018-06-12T15:12:59Z INSDC status public Submitter Id E-MTAB-6386:Sample 22 age 33 broker name ArrayExpress cell type memory B cell common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD- CD27+ IgG+ sample name E-MTAB-6386:Sample 22 scientific_name Homo sapiens sex female single cell quality OK ERS2544905 SAMEA4724768 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:55Z ENA-LAST-UPDATE 2018-06-12T15:12:59Z External Id SAMEA4724768 INSDC center name UNSW INSDC first public 2018-06-22T17:01:55Z INSDC last update 2018-06-12T15:12:59Z INSDC status public Submitter Id E-MTAB-6386:Sample 23 age 33 broker name ArrayExpress cell type memory B cell common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD- CD27+ IgG+ sample name E-MTAB-6386:Sample 23 scientific_name Homo sapiens sex female single cell quality OK ERS2544906 SAMEA4724769 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:55Z ENA-LAST-UPDATE 2018-06-12T15:12:59Z External Id SAMEA4724769 INSDC center name UNSW INSDC first public 2018-06-22T17:01:55Z INSDC last update 2018-06-12T15:12:59Z INSDC status public Submitter Id E-MTAB-6386:Sample 24 age 33 broker name ArrayExpress cell type memory B cell common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD- CD27+ IgG+ sample name E-MTAB-6386:Sample 24 scientific_name Homo sapiens sex female single cell quality OK ERS2544907 SAMEA4724770 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:55Z ENA-LAST-UPDATE 2018-06-12T15:12:59Z External Id SAMEA4724770 INSDC center name UNSW INSDC first public 2018-06-22T17:01:55Z INSDC last update 2018-06-12T15:12:59Z INSDC status public Submitter Id E-MTAB-6386:Sample 55 age 33 broker name ArrayExpress cell type memory B cell common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD- CD27+ IgG+ sample name E-MTAB-6386:Sample 55 scientific_name Homo sapiens sex female single cell quality OK ERS2544908 SAMEA4724771 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:55Z ENA-LAST-UPDATE 2018-06-12T15:12:59Z External Id SAMEA4724771 INSDC center name UNSW INSDC first public 2018-06-22T17:01:55Z INSDC last update 2018-06-12T15:12:59Z INSDC status public Submitter Id E-MTAB-6386:Sample 56 age 33 broker name ArrayExpress cell type memory B cell common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD- CD27+ IgG+ sample name E-MTAB-6386:Sample 56 scientific_name Homo sapiens sex female single cell quality OK ERS2544909 SAMEA4724772 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:55Z ENA-LAST-UPDATE 2018-06-12T15:12:59Z External Id SAMEA4724772 INSDC center name UNSW INSDC first public 2018-06-22T17:01:55Z INSDC last update 2018-06-12T15:12:59Z INSDC status public Submitter Id E-MTAB-6386:Sample 57 age 33 broker name ArrayExpress cell type memory B cell common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD- CD27+ IgG+ sample name E-MTAB-6386:Sample 57 scientific_name Homo sapiens sex female single cell quality OK ERS2544910 SAMEA4724773 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:55Z ENA-LAST-UPDATE 2018-06-12T15:12:59Z External Id SAMEA4724773 INSDC center name UNSW INSDC first public 2018-06-22T17:01:55Z INSDC last update 2018-06-12T15:12:59Z INSDC status public Submitter Id E-MTAB-6386:Sample 58 age 33 broker name ArrayExpress cell type memory B cell common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD- CD27+ IgG+ sample name E-MTAB-6386:Sample 58 scientific_name Homo sapiens sex female single cell quality OK ERS2544911 SAMEA4724774 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:55Z ENA-LAST-UPDATE 2018-06-12T15:12:59Z External Id SAMEA4724774 INSDC center name UNSW INSDC first public 2018-06-22T17:01:55Z INSDC last update 2018-06-12T15:12:59Z INSDC status public Submitter Id E-MTAB-6386:Sample 59 age 33 broker name ArrayExpress cell type memory B cell common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD- CD27+ IgG+ sample name E-MTAB-6386:Sample 59 scientific_name Homo sapiens sex female single cell quality OK ERS2544912 SAMEA4724775 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:55Z ENA-LAST-UPDATE 2018-06-12T15:12:59Z External Id SAMEA4724775 INSDC center name UNSW INSDC first public 2018-06-22T17:01:55Z INSDC last update 2018-06-12T15:12:59Z INSDC status public Submitter Id E-MTAB-6386:Sample 60 age 33 broker name ArrayExpress cell type memory B cell common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD- CD27+ IgG+ sample name E-MTAB-6386:Sample 60 scientific_name Homo sapiens sex female single cell quality OK ERS2544913 SAMEA4724776 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:55Z ENA-LAST-UPDATE 2018-06-12T15:12:59Z External Id SAMEA4724776 INSDC center name UNSW INSDC first public 2018-06-22T17:01:55Z INSDC last update 2018-06-12T15:12:59Z INSDC status public Submitter Id E-MTAB-6386:Sample 82 age 33 broker name ArrayExpress cell type memory B cell common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD- CD27+ IgG+ sample name E-MTAB-6386:Sample 82 scientific_name Homo sapiens sex female single cell quality OK ERS2544914 SAMEA4724777 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:55Z ENA-LAST-UPDATE 2018-06-12T15:12:59Z External Id SAMEA4724777 INSDC center name UNSW INSDC first public 2018-06-22T17:01:55Z INSDC last update 2018-06-12T15:12:59Z INSDC status public Submitter Id E-MTAB-6386:Sample 83 age 33 broker name ArrayExpress cell type memory B cell common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD- CD27+ IgG+ sample name E-MTAB-6386:Sample 83 scientific_name Homo sapiens sex female single cell quality OK ERS2544915 SAMEA4724778 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:55Z ENA-LAST-UPDATE 2018-06-12T15:12:59Z External Id SAMEA4724778 INSDC center name UNSW INSDC first public 2018-06-22T17:01:55Z INSDC last update 2018-06-12T15:12:59Z INSDC status public Submitter Id E-MTAB-6386:Sample 84 age 33 broker name ArrayExpress cell type memory B cell common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD- CD27+ IgG+ sample name E-MTAB-6386:Sample 84 scientific_name Homo sapiens sex female single cell quality OK ERS2544916 SAMEA4724779 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:55Z ENA-LAST-UPDATE 2018-06-12T15:12:59Z External Id SAMEA4724779 INSDC center name UNSW INSDC first public 2018-06-22T17:01:55Z INSDC last update 2018-06-12T15:12:59Z INSDC status public Submitter Id E-MTAB-6386:Sample 85 age 33 broker name ArrayExpress cell type memory B cell common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD- CD27+ IgG+ sample name E-MTAB-6386:Sample 85 scientific_name Homo sapiens sex female single cell quality OK ERS2544917 SAMEA4724780 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:55Z ENA-LAST-UPDATE 2018-06-12T15:12:59Z External Id SAMEA4724780 INSDC center name UNSW INSDC first public 2018-06-22T17:01:55Z INSDC last update 2018-06-12T15:12:59Z INSDC status public Submitter Id E-MTAB-6386:Sample 86 age 33 broker name ArrayExpress cell type memory B cell common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD- CD27+ IgG+ sample name E-MTAB-6386:Sample 86 scientific_name Homo sapiens sex female single cell quality OK ERS2544918 SAMEA4724781 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:55Z ENA-LAST-UPDATE 2018-06-12T15:12:59Z External Id SAMEA4724781 INSDC center name UNSW INSDC first public 2018-06-22T17:01:55Z INSDC last update 2018-06-12T15:12:59Z INSDC status public Submitter Id E-MTAB-6386:Sample 87 age 33 broker name ArrayExpress cell type memory B cell common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD- CD27+ IgG+ sample name E-MTAB-6386:Sample 87 scientific_name Homo sapiens sex female single cell quality OK ERS2544919 SAMEA4724782 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:55Z ENA-LAST-UPDATE 2018-06-12T15:12:59Z External Id SAMEA4724782 INSDC center name UNSW INSDC first public 2018-06-22T17:01:55Z INSDC last update 2018-06-12T15:12:59Z INSDC status public Submitter Id E-MTAB-6386:Sample 88 age 33 broker name ArrayExpress cell type memory B cell common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD- CD27+ IgG+ sample name E-MTAB-6386:Sample 88 scientific_name Homo sapiens sex female single cell quality OK ERS2544920 SAMEA4724783 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:56Z ENA-LAST-UPDATE 2018-06-12T15:12:59Z External Id SAMEA4724783 INSDC center name UNSW INSDC first public 2018-06-22T17:01:56Z INSDC last update 2018-06-12T15:12:59Z INSDC status public Submitter Id E-MTAB-6386:Sample 89 age 33 broker name ArrayExpress cell type memory B cell common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD- CD27+ IgG+ sample name E-MTAB-6386:Sample 89 scientific_name Homo sapiens sex female single cell quality OK ERS2544921 SAMEA4724784 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:56Z ENA-LAST-UPDATE 2018-06-12T15:12:59Z External Id SAMEA4724784 INSDC center name UNSW INSDC first public 2018-06-22T17:01:56Z INSDC last update 2018-06-12T15:12:59Z INSDC status public Submitter Id E-MTAB-6386:Sample 90 age 33 broker name ArrayExpress cell type memory B cell common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD- CD27+ IgG+ sample name E-MTAB-6386:Sample 90 scientific_name Homo sapiens sex female single cell quality OK ERS2544922 SAMEA4724785 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:56Z ENA-LAST-UPDATE 2018-06-12T15:12:59Z External Id SAMEA4724785 INSDC center name UNSW INSDC first public 2018-06-22T17:01:56Z INSDC last update 2018-06-12T15:12:59Z INSDC status public Submitter Id E-MTAB-6386:Sample 91 age 33 broker name ArrayExpress cell type memory B cell common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD- CD27+ IgG+ sample name E-MTAB-6386:Sample 91 scientific_name Homo sapiens sex female single cell quality OK ERS2544923 SAMEA4724786 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:56Z ENA-LAST-UPDATE 2018-06-12T15:12:59Z External Id SAMEA4724786 INSDC center name UNSW INSDC first public 2018-06-22T17:01:56Z INSDC last update 2018-06-12T15:12:59Z INSDC status public Submitter Id E-MTAB-6386:Sample 92 age 33 broker name ArrayExpress cell type memory B cell common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD- CD27+ IgG+ sample name E-MTAB-6386:Sample 92 scientific_name Homo sapiens sex female single cell quality OK ERS2544924 SAMEA4724787 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:56Z ENA-LAST-UPDATE 2018-06-12T15:12:59Z External Id SAMEA4724787 INSDC center name UNSW INSDC first public 2018-06-22T17:01:56Z INSDC last update 2018-06-12T15:12:59Z INSDC status public Submitter Id E-MTAB-6386:Sample 93 age 33 broker name ArrayExpress cell type memory B cell common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD- CD27+ IgG+ sample name E-MTAB-6386:Sample 93 scientific_name Homo sapiens sex female single cell quality OK ERS2544925 SAMEA4724788 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:56Z ENA-LAST-UPDATE 2018-06-12T15:12:59Z External Id SAMEA4724788 INSDC center name UNSW INSDC first public 2018-06-22T17:01:56Z INSDC last update 2018-06-12T15:12:59Z INSDC status public Submitter Id E-MTAB-6386:Sample 1 age 33 broker name ArrayExpress cell type naive B cell common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD+ CD27- CD10- sample name E-MTAB-6386:Sample 1 scientific_name Homo sapiens sex female single cell quality OK ERS2544926 SAMEA4724789 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:56Z ENA-LAST-UPDATE 2018-06-12T15:12:59Z External Id SAMEA4724789 INSDC center name UNSW INSDC first public 2018-06-22T17:01:56Z INSDC last update 2018-06-12T15:12:59Z INSDC status public Submitter Id E-MTAB-6386:Sample 10 age 33 broker name ArrayExpress cell type naive B cell common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD+ CD27- CD10- sample name E-MTAB-6386:Sample 10 scientific_name Homo sapiens sex female single cell quality OK ERS2544927 SAMEA4724790 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:56Z ENA-LAST-UPDATE 2018-06-12T15:12:59Z External Id SAMEA4724790 INSDC center name UNSW INSDC first public 2018-06-22T17:01:56Z INSDC last update 2018-06-12T15:12:59Z INSDC status public Submitter Id E-MTAB-6386:Sample 11 age 33 broker name ArrayExpress cell type naive B cell common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD+ CD27- CD10- sample name E-MTAB-6386:Sample 11 scientific_name Homo sapiens sex female single cell quality OK ERS2544928 SAMEA4724791 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:56Z ENA-LAST-UPDATE 2018-06-12T15:12:59Z External Id SAMEA4724791 INSDC center name UNSW INSDC first public 2018-06-22T17:01:56Z INSDC last update 2018-06-12T15:12:59Z INSDC status public Submitter Id E-MTAB-6386:Sample 12 age 33 broker name ArrayExpress cell type naive B cell common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD+ CD27- CD10- sample name E-MTAB-6386:Sample 12 scientific_name Homo sapiens sex female single cell quality OK ERS2544929 SAMEA4724792 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:56Z ENA-LAST-UPDATE 2018-06-12T15:12:59Z External Id SAMEA4724792 INSDC center name UNSW INSDC first public 2018-06-22T17:01:56Z INSDC last update 2018-06-12T15:12:59Z INSDC status public Submitter Id E-MTAB-6386:Sample 2 age 33 broker name ArrayExpress cell type naive B cell common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD+ CD27- CD10- sample name E-MTAB-6386:Sample 2 scientific_name Homo sapiens sex female single cell quality OK ERS2544930 SAMEA4724793 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:56Z ENA-LAST-UPDATE 2018-06-12T15:12:59Z External Id SAMEA4724793 INSDC center name UNSW INSDC first public 2018-06-22T17:01:56Z INSDC last update 2018-06-12T15:12:59Z INSDC status public Submitter Id E-MTAB-6386:Sample 3 age 33 broker name ArrayExpress cell type naive B cell common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD+ CD27- CD10- sample name E-MTAB-6386:Sample 3 scientific_name Homo sapiens sex female single cell quality OK ERS2544931 SAMEA4724794 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:56Z ENA-LAST-UPDATE 2018-06-12T15:12:59Z External Id SAMEA4724794 INSDC center name UNSW INSDC first public 2018-06-22T17:01:56Z INSDC last update 2018-06-12T15:12:59Z INSDC status public Submitter Id E-MTAB-6386:Sample 4 age 33 broker name ArrayExpress cell type naive B cell common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD+ CD27- CD10- sample name E-MTAB-6386:Sample 4 scientific_name Homo sapiens sex female single cell quality OK ERS2544932 SAMEA4724795 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:56Z ENA-LAST-UPDATE 2018-06-12T15:12:59Z External Id SAMEA4724795 INSDC center name UNSW INSDC first public 2018-06-22T17:01:56Z INSDC last update 2018-06-12T15:12:59Z INSDC status public Submitter Id E-MTAB-6386:Sample 49 age 33 broker name ArrayExpress cell type naive B cell common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD+ CD27- CD10- sample name E-MTAB-6386:Sample 49 scientific_name Homo sapiens sex female single cell quality OK ERS2544933 SAMEA4724796 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:56Z ENA-LAST-UPDATE 2018-06-12T15:12:59Z External Id SAMEA4724796 INSDC center name UNSW INSDC first public 2018-06-22T17:01:56Z INSDC last update 2018-06-12T15:12:59Z INSDC status public Submitter Id E-MTAB-6386:Sample 5 age 33 broker name ArrayExpress cell type naive B cell common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD+ CD27- CD10- sample name E-MTAB-6386:Sample 5 scientific_name Homo sapiens sex female single cell quality OK ERS2544934 SAMEA4724797 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:56Z ENA-LAST-UPDATE 2018-06-12T15:12:59Z External Id SAMEA4724797 INSDC center name UNSW INSDC first public 2018-06-22T17:01:56Z INSDC last update 2018-06-12T15:12:59Z INSDC status public Submitter Id E-MTAB-6386:Sample 50 age 33 broker name ArrayExpress cell type naive B cell common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD+ CD27- CD10- sample name E-MTAB-6386:Sample 50 scientific_name Homo sapiens sex female single cell quality OK ERS2544935 SAMEA4724798 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:56Z ENA-LAST-UPDATE 2018-06-12T15:12:59Z External Id SAMEA4724798 INSDC center name UNSW INSDC first public 2018-06-22T17:01:56Z INSDC last update 2018-06-12T15:12:59Z INSDC status public Submitter Id E-MTAB-6386:Sample 51 age 33 broker name ArrayExpress cell type naive B cell common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD+ CD27- CD10- sample name E-MTAB-6386:Sample 51 scientific_name Homo sapiens sex female single cell quality OK ERS2544936 SAMEA4724799 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:56Z ENA-LAST-UPDATE 2018-06-12T15:12:59Z External Id SAMEA4724799 INSDC center name UNSW INSDC first public 2018-06-22T17:01:56Z INSDC last update 2018-06-12T15:12:59Z INSDC status public Submitter Id E-MTAB-6386:Sample 52 age 33 broker name ArrayExpress cell type naive B cell common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD+ CD27- CD10- sample name E-MTAB-6386:Sample 52 scientific_name Homo sapiens sex female single cell quality OK ERS2544937 SAMEA4724800 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:56Z ENA-LAST-UPDATE 2018-06-12T15:12:59Z External Id SAMEA4724800 INSDC center name UNSW INSDC first public 2018-06-22T17:01:56Z INSDC last update 2018-06-12T15:12:59Z INSDC status public Submitter Id E-MTAB-6386:Sample 53 age 33 broker name ArrayExpress cell type naive B cell common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD+ CD27- CD10- sample name E-MTAB-6386:Sample 53 scientific_name Homo sapiens sex female single cell quality OK ERS2544938 SAMEA4724801 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:56Z ENA-LAST-UPDATE 2018-06-12T15:12:59Z External Id SAMEA4724801 INSDC center name UNSW INSDC first public 2018-06-22T17:01:56Z INSDC last update 2018-06-12T15:12:59Z INSDC status public Submitter Id E-MTAB-6386:Sample 54 age 33 broker name ArrayExpress cell type naive B cell common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD+ CD27- CD10- sample name E-MTAB-6386:Sample 54 scientific_name Homo sapiens sex female single cell quality OK ERS2544939 SAMEA4724802 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:56Z ENA-LAST-UPDATE 2018-06-12T15:12:59Z External Id SAMEA4724802 INSDC center name UNSW INSDC first public 2018-06-22T17:01:56Z INSDC last update 2018-06-12T15:12:59Z INSDC status public Submitter Id E-MTAB-6386:Sample 6 age 33 broker name ArrayExpress cell type naive B cell common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD+ CD27- CD10- sample name E-MTAB-6386:Sample 6 scientific_name Homo sapiens sex female single cell quality OK ERS2544940 SAMEA4724803 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:56Z ENA-LAST-UPDATE 2018-06-12T15:12:59Z External Id SAMEA4724803 INSDC center name UNSW INSDC first public 2018-06-22T17:01:56Z INSDC last update 2018-06-12T15:12:59Z INSDC status public Submitter Id E-MTAB-6386:Sample 7 age 33 broker name ArrayExpress cell type naive B cell common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD+ CD27- CD10- sample name E-MTAB-6386:Sample 7 scientific_name Homo sapiens sex female single cell quality OK ERS2544941 SAMEA4724804 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:56Z ENA-LAST-UPDATE 2018-06-12T15:12:59Z External Id SAMEA4724804 INSDC center name UNSW INSDC first public 2018-06-22T17:01:56Z INSDC last update 2018-06-12T15:12:59Z INSDC status public Submitter Id E-MTAB-6386:Sample 70 age 33 broker name ArrayExpress cell type naive B cell common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD+ CD27- CD10- sample name E-MTAB-6386:Sample 70 scientific_name Homo sapiens sex female single cell quality OK ERS2544942 SAMEA4724805 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:55Z ENA-LAST-UPDATE 2018-06-12T15:13:00Z External Id SAMEA4724805 INSDC center name UNSW INSDC first public 2018-06-22T17:01:55Z INSDC last update 2018-06-12T15:13:00Z INSDC status public Submitter Id E-MTAB-6386:Sample 71 age 33 broker name ArrayExpress cell type naive B cell common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD+ CD27- CD10- sample name E-MTAB-6386:Sample 71 scientific_name Homo sapiens sex female single cell quality OK ERS2544943 SAMEA4724806 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:55Z ENA-LAST-UPDATE 2018-06-12T15:13:00Z External Id SAMEA4724806 INSDC center name UNSW INSDC first public 2018-06-22T17:01:55Z INSDC last update 2018-06-12T15:13:00Z INSDC status public Submitter Id E-MTAB-6386:Sample 72 age 33 broker name ArrayExpress cell type naive B cell common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD+ CD27- CD10- sample name E-MTAB-6386:Sample 72 scientific_name Homo sapiens sex female single cell quality OK ERS2544944 SAMEA4724807 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:55Z ENA-LAST-UPDATE 2018-06-12T15:13:00Z External Id SAMEA4724807 INSDC center name UNSW INSDC first public 2018-06-22T17:01:55Z INSDC last update 2018-06-12T15:13:00Z INSDC status public Submitter Id E-MTAB-6386:Sample 73 age 33 broker name ArrayExpress cell type naive B cell common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD+ CD27- CD10- sample name E-MTAB-6386:Sample 73 scientific_name Homo sapiens sex female single cell quality OK ERS2544945 SAMEA4724808 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:55Z ENA-LAST-UPDATE 2018-06-12T15:13:00Z External Id SAMEA4724808 INSDC center name UNSW INSDC first public 2018-06-22T17:01:55Z INSDC last update 2018-06-12T15:13:00Z INSDC status public Submitter Id E-MTAB-6386:Sample 74 age 33 broker name ArrayExpress cell type naive B cell common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD+ CD27- CD10- sample name E-MTAB-6386:Sample 74 scientific_name Homo sapiens sex female single cell quality OK ERS2544946 SAMEA4724809 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:55Z ENA-LAST-UPDATE 2018-06-12T15:13:00Z External Id SAMEA4724809 INSDC center name UNSW INSDC first public 2018-06-22T17:01:55Z INSDC last update 2018-06-12T15:13:00Z INSDC status public Submitter Id E-MTAB-6386:Sample 75 age 33 broker name ArrayExpress cell type naive B cell common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD+ CD27- CD10- sample name E-MTAB-6386:Sample 75 scientific_name Homo sapiens sex female single cell quality OK ERS2544947 SAMEA4724810 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:55Z ENA-LAST-UPDATE 2018-06-12T15:13:00Z External Id SAMEA4724810 INSDC center name UNSW INSDC first public 2018-06-22T17:01:55Z INSDC last update 2018-06-12T15:13:00Z INSDC status public Submitter Id E-MTAB-6386:Sample 76 age 33 broker name ArrayExpress cell type naive B cell common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD+ CD27- CD10- sample name E-MTAB-6386:Sample 76 scientific_name Homo sapiens sex female single cell quality OK ERS2544948 SAMEA4724811 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:55Z ENA-LAST-UPDATE 2018-06-12T15:13:00Z External Id SAMEA4724811 INSDC center name UNSW INSDC first public 2018-06-22T17:01:55Z INSDC last update 2018-06-12T15:13:00Z INSDC status public Submitter Id E-MTAB-6386:Sample 77 age 33 broker name ArrayExpress cell type naive B cell common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD+ CD27- CD10- sample name E-MTAB-6386:Sample 77 scientific_name Homo sapiens sex female single cell quality OK ERS2544949 SAMEA4724812 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:55Z ENA-LAST-UPDATE 2018-06-12T15:13:00Z External Id SAMEA4724812 INSDC center name UNSW INSDC first public 2018-06-22T17:01:55Z INSDC last update 2018-06-12T15:13:00Z INSDC status public Submitter Id E-MTAB-6386:Sample 78 age 33 broker name ArrayExpress cell type naive B cell common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD+ CD27- CD10- sample name E-MTAB-6386:Sample 78 scientific_name Homo sapiens sex female single cell quality OK ERS2544950 SAMEA4724813 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:55Z ENA-LAST-UPDATE 2018-06-12T15:13:00Z External Id SAMEA4724813 INSDC center name UNSW INSDC first public 2018-06-22T17:01:55Z INSDC last update 2018-06-12T15:13:00Z INSDC status public Submitter Id E-MTAB-6386:Sample 79 age 33 broker name ArrayExpress cell type naive B cell common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD+ CD27- CD10- sample name E-MTAB-6386:Sample 79 scientific_name Homo sapiens sex female single cell quality OK ERS2544951 SAMEA4724814 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:55Z ENA-LAST-UPDATE 2018-06-12T15:13:00Z External Id SAMEA4724814 INSDC center name UNSW INSDC first public 2018-06-22T17:01:55Z INSDC last update 2018-06-12T15:13:00Z INSDC status public Submitter Id E-MTAB-6386:Sample 8 age 33 broker name ArrayExpress cell type naive B cell common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD+ CD27- CD10- sample name E-MTAB-6386:Sample 8 scientific_name Homo sapiens sex female single cell quality OK ERS2544952 SAMEA4724815 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:55Z ENA-LAST-UPDATE 2018-06-12T15:13:00Z External Id SAMEA4724815 INSDC center name UNSW INSDC first public 2018-06-22T17:01:55Z INSDC last update 2018-06-12T15:13:00Z INSDC status public Submitter Id E-MTAB-6386:Sample 80 age 33 broker name ArrayExpress cell type naive B cell common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD+ CD27- CD10- sample name E-MTAB-6386:Sample 80 scientific_name Homo sapiens sex female single cell quality OK ERS2544953 SAMEA4724816 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:55Z ENA-LAST-UPDATE 2018-06-12T15:13:00Z External Id SAMEA4724816 INSDC center name UNSW INSDC first public 2018-06-22T17:01:55Z INSDC last update 2018-06-12T15:13:00Z INSDC status public Submitter Id E-MTAB-6386:Sample 81 age 33 broker name ArrayExpress cell type naive B cell common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD+ CD27- CD10- sample name E-MTAB-6386:Sample 81 scientific_name Homo sapiens sex female single cell quality OK ERS2544954 SAMEA4724817 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:55Z ENA-LAST-UPDATE 2018-06-12T15:13:00Z External Id SAMEA4724817 INSDC center name UNSW INSDC first public 2018-06-22T17:01:55Z INSDC last update 2018-06-12T15:13:00Z INSDC status public Submitter Id E-MTAB-6386:Sample 9 age 33 broker name ArrayExpress cell type naive B cell common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD+ CD27- CD10- sample name E-MTAB-6386:Sample 9 scientific_name Homo sapiens sex female single cell quality OK ERS2544955 SAMEA4724818 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:55Z ENA-LAST-UPDATE 2018-06-12T15:13:00Z External Id SAMEA4724818 INSDC center name UNSW INSDC first public 2018-06-22T17:01:55Z INSDC last update 2018-06-12T15:13:00Z INSDC status public Submitter Id E-MTAB-6386:Sample 106 age 33 broker name ArrayExpress cell type plasmablast common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD- CD27++ CD38++ sample name E-MTAB-6386:Sample 106 scientific_name Homo sapiens sex female single cell quality OK ERS2544956 SAMEA4724819 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:55Z ENA-LAST-UPDATE 2018-06-12T15:13:00Z External Id SAMEA4724819 INSDC center name UNSW INSDC first public 2018-06-22T17:01:55Z INSDC last update 2018-06-12T15:13:00Z INSDC status public Submitter Id E-MTAB-6386:Sample 107 age 33 broker name ArrayExpress cell type plasmablast common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD- CD27++ CD38++ sample name E-MTAB-6386:Sample 107 scientific_name Homo sapiens sex female single cell quality OK ERS2544957 SAMEA4724820 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:55Z ENA-LAST-UPDATE 2018-06-12T15:13:00Z External Id SAMEA4724820 INSDC center name UNSW INSDC first public 2018-06-22T17:01:55Z INSDC last update 2018-06-12T15:13:00Z INSDC status public Submitter Id E-MTAB-6386:Sample 108 age 33 broker name ArrayExpress cell type plasmablast common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD- CD27++ CD38++ sample name E-MTAB-6386:Sample 108 scientific_name Homo sapiens sex female single cell quality OK ERS2544958 SAMEA4724821 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:55Z ENA-LAST-UPDATE 2018-06-12T15:13:00Z External Id SAMEA4724821 INSDC center name UNSW INSDC first public 2018-06-22T17:01:55Z INSDC last update 2018-06-12T15:13:00Z INSDC status public Submitter Id E-MTAB-6386:Sample 109 age 33 broker name ArrayExpress cell type plasmablast common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD- CD27++ CD38++ sample name E-MTAB-6386:Sample 109 scientific_name Homo sapiens sex female single cell quality OK ERS2544959 SAMEA4724822 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:55Z ENA-LAST-UPDATE 2018-06-12T15:13:00Z External Id SAMEA4724822 INSDC center name UNSW INSDC first public 2018-06-22T17:01:55Z INSDC last update 2018-06-12T15:13:00Z INSDC status public Submitter Id E-MTAB-6386:Sample 110 age 33 broker name ArrayExpress cell type plasmablast common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD- CD27++ CD38++ sample name E-MTAB-6386:Sample 110 scientific_name Homo sapiens sex female single cell quality OK ERS2544960 SAMEA4724823 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:55Z ENA-LAST-UPDATE 2018-06-12T15:13:00Z External Id SAMEA4724823 INSDC center name UNSW INSDC first public 2018-06-22T17:01:55Z INSDC last update 2018-06-12T15:13:00Z INSDC status public Submitter Id E-MTAB-6386:Sample 111 age 33 broker name ArrayExpress cell type plasmablast common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD- CD27++ CD38++ sample name E-MTAB-6386:Sample 111 scientific_name Homo sapiens sex female single cell quality OK ERS2544961 SAMEA4724824 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:55Z ENA-LAST-UPDATE 2018-06-12T15:13:00Z External Id SAMEA4724824 INSDC center name UNSW INSDC first public 2018-06-22T17:01:55Z INSDC last update 2018-06-12T15:13:00Z INSDC status public Submitter Id E-MTAB-6386:Sample 112 age 33 broker name ArrayExpress cell type plasmablast common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD- CD27++ CD38++ sample name E-MTAB-6386:Sample 112 scientific_name Homo sapiens sex female single cell quality OK ERS2544962 SAMEA4724825 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:55Z ENA-LAST-UPDATE 2018-06-12T15:13:00Z External Id SAMEA4724825 INSDC center name UNSW INSDC first public 2018-06-22T17:01:55Z INSDC last update 2018-06-12T15:13:00Z INSDC status public Submitter Id E-MTAB-6386:Sample 113 age 33 broker name ArrayExpress cell type plasmablast common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD- CD27++ CD38++ sample name E-MTAB-6386:Sample 113 scientific_name Homo sapiens sex female single cell quality OK ERS2544963 SAMEA4724826 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:55Z ENA-LAST-UPDATE 2018-06-12T15:13:00Z External Id SAMEA4724826 INSDC center name UNSW INSDC first public 2018-06-22T17:01:55Z INSDC last update 2018-06-12T15:13:00Z INSDC status public Submitter Id E-MTAB-6386:Sample 114 age 33 broker name ArrayExpress cell type plasmablast common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD- CD27++ CD38++ sample name E-MTAB-6386:Sample 114 scientific_name Homo sapiens sex female single cell quality OK ERS2544964 SAMEA4724827 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:55Z ENA-LAST-UPDATE 2018-06-12T15:13:00Z External Id SAMEA4724827 INSDC center name UNSW INSDC first public 2018-06-22T17:01:55Z INSDC last update 2018-06-12T15:13:00Z INSDC status public Submitter Id E-MTAB-6386:Sample 115 age 33 broker name ArrayExpress cell type plasmablast common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD- CD27++ CD38++ sample name E-MTAB-6386:Sample 115 scientific_name Homo sapiens sex female single cell quality OK ERS2544965 SAMEA4724828 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:55Z ENA-LAST-UPDATE 2018-06-12T15:13:00Z External Id SAMEA4724828 INSDC center name UNSW INSDC first public 2018-06-22T17:01:55Z INSDC last update 2018-06-12T15:13:00Z INSDC status public Submitter Id E-MTAB-6386:Sample 116 age 33 broker name ArrayExpress cell type plasmablast common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD- CD27++ CD38++ sample name E-MTAB-6386:Sample 116 scientific_name Homo sapiens sex female single cell quality OK ERS2544966 SAMEA4724829 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:55Z ENA-LAST-UPDATE 2018-06-12T15:13:00Z External Id SAMEA4724829 INSDC center name UNSW INSDC first public 2018-06-22T17:01:55Z INSDC last update 2018-06-12T15:13:00Z INSDC status public Submitter Id E-MTAB-6386:Sample 117 age 33 broker name ArrayExpress cell type plasmablast common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD- CD27++ CD38++ sample name E-MTAB-6386:Sample 117 scientific_name Homo sapiens sex female single cell quality OK ERS2544967 SAMEA4724830 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:55Z ENA-LAST-UPDATE 2018-06-12T15:13:00Z External Id SAMEA4724830 INSDC center name UNSW INSDC first public 2018-06-22T17:01:55Z INSDC last update 2018-06-12T15:13:00Z INSDC status public Submitter Id E-MTAB-6386:Sample 37 age 33 broker name ArrayExpress cell type plasmablast common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD- CD27++ CD38++ sample name E-MTAB-6386:Sample 37 scientific_name Homo sapiens sex female single cell quality OK ERS2544968 SAMEA4724831 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:55Z ENA-LAST-UPDATE 2018-06-12T15:13:00Z External Id SAMEA4724831 INSDC center name UNSW INSDC first public 2018-06-22T17:01:55Z INSDC last update 2018-06-12T15:13:00Z INSDC status public Submitter Id E-MTAB-6386:Sample 38 age 33 broker name ArrayExpress cell type plasmablast common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD- CD27++ CD38++ sample name E-MTAB-6386:Sample 38 scientific_name Homo sapiens sex female single cell quality OK ERS2544969 SAMEA4724832 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:55Z ENA-LAST-UPDATE 2018-06-12T15:13:00Z External Id SAMEA4724832 INSDC center name UNSW INSDC first public 2018-06-22T17:01:55Z INSDC last update 2018-06-12T15:13:00Z INSDC status public Submitter Id E-MTAB-6386:Sample 39 age 33 broker name ArrayExpress cell type plasmablast common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD- CD27++ CD38++ sample name E-MTAB-6386:Sample 39 scientific_name Homo sapiens sex female single cell quality OK ERS2544970 SAMEA4724833 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:55Z ENA-LAST-UPDATE 2018-06-12T15:13:00Z External Id SAMEA4724833 INSDC center name UNSW INSDC first public 2018-06-22T17:01:55Z INSDC last update 2018-06-12T15:13:00Z INSDC status public Submitter Id E-MTAB-6386:Sample 40 age 33 broker name ArrayExpress cell type plasmablast common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD- CD27++ CD38++ sample name E-MTAB-6386:Sample 40 scientific_name Homo sapiens sex female single cell quality OK ERS2544971 SAMEA4724834 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:55Z ENA-LAST-UPDATE 2018-06-12T15:13:00Z External Id SAMEA4724834 INSDC center name UNSW INSDC first public 2018-06-22T17:01:55Z INSDC last update 2018-06-12T15:13:00Z INSDC status public Submitter Id E-MTAB-6386:Sample 41 age 33 broker name ArrayExpress cell type plasmablast common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD- CD27++ CD38++ sample name E-MTAB-6386:Sample 41 scientific_name Homo sapiens sex female single cell quality OK ERS2544972 SAMEA4724835 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:55Z ENA-LAST-UPDATE 2018-06-12T15:13:00Z External Id SAMEA4724835 INSDC center name UNSW INSDC first public 2018-06-22T17:01:55Z INSDC last update 2018-06-12T15:13:00Z INSDC status public Submitter Id E-MTAB-6386:Sample 42 age 33 broker name ArrayExpress cell type plasmablast common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD- CD27++ CD38++ sample name E-MTAB-6386:Sample 42 scientific_name Homo sapiens sex female single cell quality OK ERS2544973 SAMEA4724836 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:55Z ENA-LAST-UPDATE 2018-06-12T15:13:00Z External Id SAMEA4724836 INSDC center name UNSW INSDC first public 2018-06-22T17:01:55Z INSDC last update 2018-06-12T15:13:00Z INSDC status public Submitter Id E-MTAB-6386:Sample 43 age 33 broker name ArrayExpress cell type plasmablast common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD- CD27++ CD38++ sample name E-MTAB-6386:Sample 43 scientific_name Homo sapiens sex female single cell quality OK ERS2544974 SAMEA4724837 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:55Z ENA-LAST-UPDATE 2018-06-12T15:13:00Z External Id SAMEA4724837 INSDC center name UNSW INSDC first public 2018-06-22T17:01:55Z INSDC last update 2018-06-12T15:13:00Z INSDC status public Submitter Id E-MTAB-6386:Sample 44 age 33 broker name ArrayExpress cell type plasmablast common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD- CD27++ CD38++ sample name E-MTAB-6386:Sample 44 scientific_name Homo sapiens sex female single cell quality OK ERS2544975 SAMEA4724838 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:55Z ENA-LAST-UPDATE 2018-06-12T15:13:00Z External Id SAMEA4724838 INSDC center name UNSW INSDC first public 2018-06-22T17:01:55Z INSDC last update 2018-06-12T15:13:00Z INSDC status public Submitter Id E-MTAB-6386:Sample 45 age 33 broker name ArrayExpress cell type plasmablast common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD- CD27++ CD38++ sample name E-MTAB-6386:Sample 45 scientific_name Homo sapiens sex female single cell quality OK ERS2544976 SAMEA4724839 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:55Z ENA-LAST-UPDATE 2018-06-12T15:13:00Z External Id SAMEA4724839 INSDC center name UNSW INSDC first public 2018-06-22T17:01:55Z INSDC last update 2018-06-12T15:13:00Z INSDC status public Submitter Id E-MTAB-6386:Sample 46 age 33 broker name ArrayExpress cell type plasmablast common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD- CD27++ CD38++ sample name E-MTAB-6386:Sample 46 scientific_name Homo sapiens sex female single cell quality OK ERS2544977 SAMEA4724840 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:55Z ENA-LAST-UPDATE 2018-06-12T15:13:00Z External Id SAMEA4724840 INSDC center name UNSW INSDC first public 2018-06-22T17:01:55Z INSDC last update 2018-06-12T15:13:00Z INSDC status public Submitter Id E-MTAB-6386:Sample 47 age 33 broker name ArrayExpress cell type plasmablast common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD- CD27++ CD38++ sample name E-MTAB-6386:Sample 47 scientific_name Homo sapiens sex female single cell quality OK ERS2544978 SAMEA4724841 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:55Z ENA-LAST-UPDATE 2018-06-12T15:13:00Z External Id SAMEA4724841 INSDC center name UNSW INSDC first public 2018-06-22T17:01:55Z INSDC last update 2018-06-12T15:13:00Z INSDC status public Submitter Id E-MTAB-6386:Sample 48 age 33 broker name ArrayExpress cell type plasmablast common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD- CD27++ CD38++ sample name E-MTAB-6386:Sample 48 scientific_name Homo sapiens sex female single cell quality OK ERS2544979 SAMEA4724842 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:55Z ENA-LAST-UPDATE 2018-06-12T15:13:00Z External Id SAMEA4724842 INSDC center name UNSW INSDC first public 2018-06-22T17:01:55Z INSDC last update 2018-06-12T15:13:00Z INSDC status public Submitter Id E-MTAB-6386:Sample 64 age 33 broker name ArrayExpress cell type plasmablast common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD- CD27++ CD38++ sample name E-MTAB-6386:Sample 64 scientific_name Homo sapiens sex female single cell quality OK ERS2544980 SAMEA4724843 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:55Z ENA-LAST-UPDATE 2018-06-12T15:13:00Z External Id SAMEA4724843 INSDC center name UNSW INSDC first public 2018-06-22T17:01:55Z INSDC last update 2018-06-12T15:13:00Z INSDC status public Submitter Id E-MTAB-6386:Sample 65 age 33 broker name ArrayExpress cell type plasmablast common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD- CD27++ CD38++ sample name E-MTAB-6386:Sample 65 scientific_name Homo sapiens sex female single cell quality OK ERS2544981 SAMEA4724844 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:55Z ENA-LAST-UPDATE 2018-06-12T15:13:00Z External Id SAMEA4724844 INSDC center name UNSW INSDC first public 2018-06-22T17:01:55Z INSDC last update 2018-06-12T15:13:00Z INSDC status public Submitter Id E-MTAB-6386:Sample 66 age 33 broker name ArrayExpress cell type plasmablast common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD- CD27++ CD38++ sample name E-MTAB-6386:Sample 66 scientific_name Homo sapiens sex female single cell quality OK ERS2544982 SAMEA4724845 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:55Z ENA-LAST-UPDATE 2018-06-12T15:13:00Z External Id SAMEA4724845 INSDC center name UNSW INSDC first public 2018-06-22T17:01:55Z INSDC last update 2018-06-12T15:13:00Z INSDC status public Submitter Id E-MTAB-6386:Sample 67 age 33 broker name ArrayExpress cell type plasmablast common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD- CD27++ CD38++ sample name E-MTAB-6386:Sample 67 scientific_name Homo sapiens sex female single cell quality OK ERS2544983 SAMEA4724846 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:55Z ENA-LAST-UPDATE 2018-06-12T15:13:00Z External Id SAMEA4724846 INSDC center name UNSW INSDC first public 2018-06-22T17:01:55Z INSDC last update 2018-06-12T15:13:00Z INSDC status public Submitter Id E-MTAB-6386:Sample 68 age 33 broker name ArrayExpress cell type plasmablast common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD- CD27++ CD38++ sample name E-MTAB-6386:Sample 68 scientific_name Homo sapiens sex female single cell quality OK ERS2544984 SAMEA4724847 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:55Z ENA-LAST-UPDATE 2018-06-12T15:13:00Z External Id SAMEA4724847 INSDC center name UNSW INSDC first public 2018-06-22T17:01:55Z INSDC last update 2018-06-12T15:13:00Z INSDC status public Submitter Id E-MTAB-6386:Sample 69 age 33 broker name ArrayExpress cell type plasmablast common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD- CD27++ CD38++ sample name E-MTAB-6386:Sample 69 scientific_name Homo sapiens sex female single cell quality OK ERS2544985 SAMEA4724848 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:55Z ENA-LAST-UPDATE 2018-06-12T15:13:01Z External Id SAMEA4724848 INSDC center name UNSW INSDC first public 2018-06-22T17:01:55Z INSDC last update 2018-06-12T15:13:01Z INSDC status public Submitter Id E-MTAB-6386:Sample 100 age 33 broker name ArrayExpress cell type transitional stage B cell common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD+ CD27- CD10+ sample name E-MTAB-6386:Sample 100 scientific_name Homo sapiens sex female single cell quality OK ERS2544986 SAMEA4724849 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:55Z ENA-LAST-UPDATE 2018-06-12T15:13:01Z External Id SAMEA4724849 INSDC center name UNSW INSDC first public 2018-06-22T17:01:55Z INSDC last update 2018-06-12T15:13:01Z INSDC status public Submitter Id E-MTAB-6386:Sample 101 age 33 broker name ArrayExpress cell type transitional stage B cell common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD+ CD27- CD10+ sample name E-MTAB-6386:Sample 101 scientific_name Homo sapiens sex female single cell quality OK ERS2544987 SAMEA4724850 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:55Z ENA-LAST-UPDATE 2018-06-12T15:13:01Z External Id SAMEA4724850 INSDC center name UNSW INSDC first public 2018-06-22T17:01:55Z INSDC last update 2018-06-12T15:13:01Z INSDC status public Submitter Id E-MTAB-6386:Sample 102 age 33 broker name ArrayExpress cell type transitional stage B cell common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD+ CD27- CD10+ sample name E-MTAB-6386:Sample 102 scientific_name Homo sapiens sex female single cell quality OK ERS2544988 SAMEA4724851 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:55Z ENA-LAST-UPDATE 2018-06-12T15:13:01Z External Id SAMEA4724851 INSDC center name UNSW INSDC first public 2018-06-22T17:01:55Z INSDC last update 2018-06-12T15:13:01Z INSDC status public Submitter Id E-MTAB-6386:Sample 103 age 33 broker name ArrayExpress cell type transitional stage B cell common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD+ CD27- CD10+ sample name E-MTAB-6386:Sample 103 scientific_name Homo sapiens sex female single cell quality OK ERS2544989 SAMEA4724852 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:55Z ENA-LAST-UPDATE 2018-06-12T15:13:01Z External Id SAMEA4724852 INSDC center name UNSW INSDC first public 2018-06-22T17:01:55Z INSDC last update 2018-06-12T15:13:01Z INSDC status public Submitter Id E-MTAB-6386:Sample 104 age 33 broker name ArrayExpress cell type transitional stage B cell common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD+ CD27- CD10+ sample name E-MTAB-6386:Sample 104 scientific_name Homo sapiens sex female single cell quality OK ERS2544990 SAMEA4724853 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:55Z ENA-LAST-UPDATE 2018-06-12T15:13:01Z External Id SAMEA4724853 INSDC center name UNSW INSDC first public 2018-06-22T17:01:55Z INSDC last update 2018-06-12T15:13:01Z INSDC status public Submitter Id E-MTAB-6386:Sample 105 age 33 broker name ArrayExpress cell type transitional stage B cell common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD+ CD27- CD10+ sample name E-MTAB-6386:Sample 105 scientific_name Homo sapiens sex female single cell quality OK ERS2544991 SAMEA4724854 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:55Z ENA-LAST-UPDATE 2018-06-12T15:13:01Z External Id SAMEA4724854 INSDC center name UNSW INSDC first public 2018-06-22T17:01:55Z INSDC last update 2018-06-12T15:13:01Z INSDC status public Submitter Id E-MTAB-6386:Sample 25 age 33 broker name ArrayExpress cell type transitional stage B cell common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD+ CD27- CD10+ sample name E-MTAB-6386:Sample 25 scientific_name Homo sapiens sex female single cell quality OK ERS2544992 SAMEA4724855 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:55Z ENA-LAST-UPDATE 2018-06-12T15:13:01Z External Id SAMEA4724855 INSDC center name UNSW INSDC first public 2018-06-22T17:01:55Z INSDC last update 2018-06-12T15:13:01Z INSDC status public Submitter Id E-MTAB-6386:Sample 26 age 33 broker name ArrayExpress cell type transitional stage B cell common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD+ CD27- CD10+ sample name E-MTAB-6386:Sample 26 scientific_name Homo sapiens sex female single cell quality OK ERS2544993 SAMEA4724856 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:55Z ENA-LAST-UPDATE 2018-06-12T15:13:01Z External Id SAMEA4724856 INSDC center name UNSW INSDC first public 2018-06-22T17:01:55Z INSDC last update 2018-06-12T15:13:01Z INSDC status public Submitter Id E-MTAB-6386:Sample 27 age 33 broker name ArrayExpress cell type transitional stage B cell common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD+ CD27- CD10+ sample name E-MTAB-6386:Sample 27 scientific_name Homo sapiens sex female single cell quality OK ERS2544994 SAMEA4724857 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:55Z ENA-LAST-UPDATE 2018-06-12T15:13:01Z External Id SAMEA4724857 INSDC center name UNSW INSDC first public 2018-06-22T17:01:55Z INSDC last update 2018-06-12T15:13:01Z INSDC status public Submitter Id E-MTAB-6386:Sample 28 age 33 broker name ArrayExpress cell type transitional stage B cell common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD+ CD27- CD10+ sample name E-MTAB-6386:Sample 28 scientific_name Homo sapiens sex female single cell quality OK ERS2544995 SAMEA4724858 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:55Z ENA-LAST-UPDATE 2018-06-12T15:13:01Z External Id SAMEA4724858 INSDC center name UNSW INSDC first public 2018-06-22T17:01:55Z INSDC last update 2018-06-12T15:13:01Z INSDC status public Submitter Id E-MTAB-6386:Sample 29 age 33 broker name ArrayExpress cell type transitional stage B cell common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD+ CD27- CD10+ sample name E-MTAB-6386:Sample 29 scientific_name Homo sapiens sex female single cell quality OK ERS2544996 SAMEA4724859 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:55Z ENA-LAST-UPDATE 2018-06-12T15:13:01Z External Id SAMEA4724859 INSDC center name UNSW INSDC first public 2018-06-22T17:01:55Z INSDC last update 2018-06-12T15:13:01Z INSDC status public Submitter Id E-MTAB-6386:Sample 30 age 33 broker name ArrayExpress cell type transitional stage B cell common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD+ CD27- CD10+ sample name E-MTAB-6386:Sample 30 scientific_name Homo sapiens sex female single cell quality OK ERS2544997 SAMEA4724860 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:56Z ENA-LAST-UPDATE 2018-06-12T15:13:01Z External Id SAMEA4724860 INSDC center name UNSW INSDC first public 2018-06-22T17:01:56Z INSDC last update 2018-06-12T15:13:01Z INSDC status public Submitter Id E-MTAB-6386:Sample 31 age 33 broker name ArrayExpress cell type transitional stage B cell common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD+ CD27- CD10+ sample name E-MTAB-6386:Sample 31 scientific_name Homo sapiens sex female single cell quality OK ERS2544998 SAMEA4724861 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:56Z ENA-LAST-UPDATE 2018-06-12T15:13:01Z External Id SAMEA4724861 INSDC center name UNSW INSDC first public 2018-06-22T17:01:56Z INSDC last update 2018-06-12T15:13:01Z INSDC status public Submitter Id E-MTAB-6386:Sample 32 age 33 broker name ArrayExpress cell type transitional stage B cell common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD+ CD27- CD10+ sample name E-MTAB-6386:Sample 32 scientific_name Homo sapiens sex female single cell quality OK ERS2544999 SAMEA4724862 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:56Z ENA-LAST-UPDATE 2018-06-12T15:13:01Z External Id SAMEA4724862 INSDC center name UNSW INSDC first public 2018-06-22T17:01:56Z INSDC last update 2018-06-12T15:13:01Z INSDC status public Submitter Id E-MTAB-6386:Sample 33 age 33 broker name ArrayExpress cell type transitional stage B cell common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD+ CD27- CD10+ sample name E-MTAB-6386:Sample 33 scientific_name Homo sapiens sex female single cell quality OK ERS2545000 SAMEA4724863 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:56Z ENA-LAST-UPDATE 2018-06-12T15:13:01Z External Id SAMEA4724863 INSDC center name UNSW INSDC first public 2018-06-22T17:01:56Z INSDC last update 2018-06-12T15:13:01Z INSDC status public Submitter Id E-MTAB-6386:Sample 34 age 33 broker name ArrayExpress cell type transitional stage B cell common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD+ CD27- CD10+ sample name E-MTAB-6386:Sample 34 scientific_name Homo sapiens sex female single cell quality OK ERS2545001 SAMEA4724864 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:56Z ENA-LAST-UPDATE 2018-06-12T15:13:01Z External Id SAMEA4724864 INSDC center name UNSW INSDC first public 2018-06-22T17:01:56Z INSDC last update 2018-06-12T15:13:01Z INSDC status public Submitter Id E-MTAB-6386:Sample 35 age 33 broker name ArrayExpress cell type transitional stage B cell common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD+ CD27- CD10+ sample name E-MTAB-6386:Sample 35 scientific_name Homo sapiens sex female single cell quality OK ERS2545002 SAMEA4724865 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:56Z ENA-LAST-UPDATE 2018-06-12T15:13:01Z External Id SAMEA4724865 INSDC center name UNSW INSDC first public 2018-06-22T17:01:56Z INSDC last update 2018-06-12T15:13:01Z INSDC status public Submitter Id E-MTAB-6386:Sample 36 age 33 broker name ArrayExpress cell type transitional stage B cell common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD+ CD27- CD10+ sample name E-MTAB-6386:Sample 36 scientific_name Homo sapiens sex female single cell quality OK ERS2545003 SAMEA4724866 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:56Z ENA-LAST-UPDATE 2018-06-12T15:13:01Z External Id SAMEA4724866 INSDC center name UNSW INSDC first public 2018-06-22T17:01:56Z INSDC last update 2018-06-12T15:13:01Z INSDC status public Submitter Id E-MTAB-6386:Sample 61 age 33 broker name ArrayExpress cell type transitional stage B cell common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD+ CD27- CD10+ sample name E-MTAB-6386:Sample 61 scientific_name Homo sapiens sex female single cell quality OK ERS2545004 SAMEA4724867 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:56Z ENA-LAST-UPDATE 2018-06-12T15:13:01Z External Id SAMEA4724867 INSDC center name UNSW INSDC first public 2018-06-22T17:01:56Z INSDC last update 2018-06-12T15:13:01Z INSDC status public Submitter Id E-MTAB-6386:Sample 62 age 33 broker name ArrayExpress cell type transitional stage B cell common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD+ CD27- CD10+ sample name E-MTAB-6386:Sample 62 scientific_name Homo sapiens sex female single cell quality OK ERS2545005 SAMEA4724868 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:56Z ENA-LAST-UPDATE 2018-06-12T15:13:01Z External Id SAMEA4724868 INSDC center name UNSW INSDC first public 2018-06-22T17:01:56Z INSDC last update 2018-06-12T15:13:01Z INSDC status public Submitter Id E-MTAB-6386:Sample 63 age 33 broker name ArrayExpress cell type transitional stage B cell common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD+ CD27- CD10+ sample name E-MTAB-6386:Sample 63 scientific_name Homo sapiens sex female single cell quality OK ERS2545006 SAMEA4724869 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:56Z ENA-LAST-UPDATE 2018-06-12T15:13:01Z External Id SAMEA4724869 INSDC center name UNSW INSDC first public 2018-06-22T17:01:56Z INSDC last update 2018-06-12T15:13:01Z INSDC status public Submitter Id E-MTAB-6386:Sample 94 age 33 broker name ArrayExpress cell type transitional stage B cell common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD+ CD27- CD10+ sample name E-MTAB-6386:Sample 94 scientific_name Homo sapiens sex female single cell quality OK ERS2545007 SAMEA4724870 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:56Z ENA-LAST-UPDATE 2018-06-12T15:13:01Z External Id SAMEA4724870 INSDC center name UNSW INSDC first public 2018-06-22T17:01:56Z INSDC last update 2018-06-12T15:13:01Z INSDC status public Submitter Id E-MTAB-6386:Sample 95 age 33 broker name ArrayExpress cell type transitional stage B cell common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD+ CD27- CD10+ sample name E-MTAB-6386:Sample 95 scientific_name Homo sapiens sex female single cell quality OK ERS2545008 SAMEA4724871 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:56Z ENA-LAST-UPDATE 2018-06-12T15:13:01Z External Id SAMEA4724871 INSDC center name UNSW INSDC first public 2018-06-22T17:01:56Z INSDC last update 2018-06-12T15:13:01Z INSDC status public Submitter Id E-MTAB-6386:Sample 96 age 33 broker name ArrayExpress cell type transitional stage B cell common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD+ CD27- CD10+ sample name E-MTAB-6386:Sample 96 scientific_name Homo sapiens sex female single cell quality OK ERS2545009 SAMEA4724872 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:56Z ENA-LAST-UPDATE 2018-06-12T15:13:01Z External Id SAMEA4724872 INSDC center name UNSW INSDC first public 2018-06-22T17:01:56Z INSDC last update 2018-06-12T15:13:01Z INSDC status public Submitter Id E-MTAB-6386:Sample 97 age 33 broker name ArrayExpress cell type transitional stage B cell common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD+ CD27- CD10+ sample name E-MTAB-6386:Sample 97 scientific_name Homo sapiens sex female single cell quality OK ERS2545010 SAMEA4724873 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:56Z ENA-LAST-UPDATE 2018-06-12T15:13:01Z External Id SAMEA4724873 INSDC center name UNSW INSDC first public 2018-06-22T17:01:56Z INSDC last update 2018-06-12T15:13:01Z INSDC status public Submitter Id E-MTAB-6386:Sample 98 age 33 broker name ArrayExpress cell type transitional stage B cell common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD+ CD27- CD10+ sample name E-MTAB-6386:Sample 98 scientific_name Homo sapiens sex female single cell quality OK ERS2545011 SAMEA4724874 9606 Homo sapiens Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume ENA-FIRST-PUBLIC 2018-06-22T17:01:56Z ENA-LAST-UPDATE 2018-06-12T15:13:01Z External Id SAMEA4724874 INSDC center name UNSW INSDC first public 2018-06-22T17:01:56Z INSDC last update 2018-06-12T15:13:01Z INSDC status public Submitter Id E-MTAB-6386:Sample 99 age 33 broker name ArrayExpress cell type transitional stage B cell common name human developmental stage adult disease normal individual A organism part blood phenotype CD19+ IgD+ CD27- CD10+ sample name E-MTAB-6386:Sample 99 scientific_name Homo sapiens sex female single cell quality OK